Abstract:
:Four monoclonal anti-parathyroid antibodies were generated after immunization of mice with intact cells from human parathyroid tissue. All four monoclonal antibodies reacted in immunohistochemistry with structures present on parathyroid epithelial cells and proximal-tubule cells of the kidney but were unreactive with all other human tissues investigated. Immunofluorescence microscopy on suspended human parathyroid cells showed that the antibodies reacted with structures present on the cell surface. Two of these antibodies efficiently blocked the increase in cytoplasmic calcium concentration of parathyroid cells that is normally associated with increased concentrations of extracellular calcium. The results indicate that these two antibodies interfere with a calcium-sensing mechanism of parathyroid cells--i.e., a potential calcium receptor by which extracellular calcium regulates cytoplasmic calcium and hormone release in these cells.
journal_name
Proc Natl Acad Sci U S Aauthors
Juhlin C,Holmdahl R,Johansson H,Rastad J,Akerström G,Klareskog Ldoi
10.1073/pnas.84.9.2990subject
Has Abstractpub_date
1987-05-01 00:00:00pages
2990-4issue
9eissn
0027-8424issn
1091-6490journal_volume
84pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2014-09-09 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1988-12-01 00:00:00