Targeted overexpression of protein kinase C beta2 isoform in myocardium causes cardiomyopathy.

abstract：:We have isolated and characterized the complete human villin gene. The villin gene is located on chromosome 2q35-36 in humans and on chromosome 1 in mice. Villin belongs to a family of calcium-regulated actin-binding proteins that share structural and functional homologies. The villin gene is expressed mainly in cells...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pringault E,Robine S,Louvard D

更新日期：1991-12-01 00:00:00

abstract：:Increased intestinal permeability has been observed in numerous human autoimmune diseases, including type-1 diabetes (T1D) and its' animal model, the BB-wor diabetic prone rat. We have recently described zonulin, a protein that regulates intercellular tight junctions. The objective of this study was to establish wheth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watts T,Berti I,Sapone A,Gerarduzzi T,Not T,Zielke R,Fasano A

更新日期：2005-02-22 00:00:00

abstract：:Understanding and manipulation of the forces assembling DNA/RNA helices have broad implications for biology, medicine, and physics. One subject of significance is the attractive force between dsDNA mediated by polycations of valence ≥ 3. Despite extensive studies, the physical origin of the "like-charge attraction" re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qiu X,Parsegian VA,Rau DC

更新日期：2010-12-14 00:00:00

abstract：:The major histocompatibility complex class-I (MHC-I) peptide-loading complex (PLC) is a cornerstone of the human adaptive immune system, being responsible for processing antigens that allow killer T cells to distinguish between healthy and compromised cells. Based on a recent low-resolution cryo-electron microscopy (c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fisette O,Schröder GF,Schäfer LV

更新日期：2020-08-25 00:00:00

abstract：:A stable free-radical polymeric derivative of prostaglandin B1 (PGBx) has been synthesized that exhibits regenerative effects on oxidative phosphorylation in aged mitochondria. The molecular weights of the most active preparations fall between 2000 and 2600. PGBx is characterized by a single-line electron spin resonan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Polis BD,Polis E,Kwong S

更新日期：1979-04-01 00:00:00

abstract：:Cointegrate DNA molecules containing two copies of a transposable element appear to be intermediates in the transposition process. These structures are resolved by site-specific recombination to yield the normal end products of transposition. The transposable element gamma delta (Tn1000) synthesizes a product intercha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kitts P,Symington L,Burke M,Reed R,Sherratt D

更新日期：1982-01-01 00:00:00

abstract：:A density-shift method is described for analyzing insulin receptor synthesis and turnover in cultured cells labeled with "heavy" amino acids (2H, 13C, and 15N). Solubilized newly synthesized heavy and old "light" receptors are separated by isopycnic banding on CsCl gradients and then quantitated. Insulin receptor synt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reed BC,Lane MD

更新日期：1980-01-01 00:00:00

abstract：:Adeno-associated virus (AAV) is a small human Dependovirus whose low immunogenicity and capacity for long-term persistence have led to its widespread use as vector for gene therapy. Despite great recent successes in AAV-based gene therapy, further improvements in vector technology may be hindered by an inadequate unde...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith-Moore S,Neil SJD,Fraefel C,Linden RM,Bollen M,Rowe HM,Henckaerts E

更新日期：2018-04-10 00:00:00

abstract：:Phosphatidylinositol (3,4,5)-triphosphate (PtdInsP(3)) mediates intracellular signaling for directional sensing and pseudopod extension at the leading edge of migrating cells during chemotaxis. How this PtdInsP(3) signal is translated into remodeling of the actin cytoskeleton is poorly understood. Here, using a proteo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang P,Wang Y,Sesaki H,Iijima M

更新日期：2010-06-29 00:00:00

abstract：:Chemokines play a key role in inflammation. They are expressed not only in neuroinflammatory conditions, but also constitutively by different cell types, including neurons in the normal brain, suggesting that they may act as modulators of neuronal functions. Here, we investigated a possible neuroendocrine role of the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Callewaere C,Banisadr G,Desarménien MG,Mechighel P,Kitabgi P,Rostène WH,Mélik Parsadaniantz S

更新日期：2006-05-23 00:00:00

abstract：:The global demand for food could double in another 40 y owing to growth in the population and food consumption per capita. To meet the world's future food and sustainability needs for biofuels and renewable materials, the production of starch-rich cereals and cellulose-rich bioenergy plants must grow substantially whi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： You C,Chen H,Myung S,Sathitsuksanoh N,Ma H,Zhang XZ,Li J,Zhang YH

更新日期：2013-04-30 00:00:00

abstract：:Allostery, the coupling between ligand binding and protein conformational change, is the heart of biological network and it has often been explained by two representative models, the induced-fit and the population-shift models. Here, we clarified for what systems one model fits better than the other by performing mole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okazaki K,Takada S

更新日期：2008-08-12 00:00:00

abstract：:LMB-7 [B3(Fv)-PE38] is a single-chain immunotoxin constructed from the murine monoclonal antibody B3 and a truncated from of Pseudomonas exotoxin PE38. Antibody B3 recognizes a carbohydrate epitope found on solid tumors that frequently invade the intrathecal space and cause neoplastic meningitis. We tested the therape...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pastan IH,Archer GE,McLendon RE,Friedman HS,Fuchs HE,Wang QC,Pai LH,Herndon J,Bigner DD

更新日期：1995-03-28 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is an emerging cause of catheter-associated urinary tract infection (CAUTI), which frequently progresses to more serious invasive infections. We adapted a mouse model of CAUTI to investigate how catheterization increases an individual's susceptibility to MRSA UTI. Thi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walker JN,Flores-Mireles AL,Pinkner CL,Schreiber HL 4th,Joens MS,Park AM,Potretzke AM,Bauman TM,Pinkner JS,Fitzpatrick JAJ,Desai A,Caparon MG,Hultgren SJ

更新日期：2017-10-10 00:00:00

abstract：:We study the distributions of citations received by a single publication within several disciplines, spanning broad areas of science. We show that the probability that an article is cited c times has large variations between different disciplines, but all distributions are rescaled on a universal curve when the relati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Radicchi F,Fortunato S,Castellano C

更新日期：2008-11-11 00:00:00

abstract：:G protein-gated inwardly rectifying potassium (GIRK) channels are critical regulators of neuronal excitability and can be directly activated by ethanol. Constitutive deletion of the GIRK3 subunit has minimal phenotypic consequences, except in response to drugs of abuse. Here we investigated how the GIRK3 subunit contr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Herman MA,Sidhu H,Stouffer DG,Kreifeldt M,Le D,Cates-Gatto C,Munoz MB,Roberts AJ,Parsons LH,Roberto M,Wickman K,Slesinger PA,Contet C

更新日期：2015-06-02 00:00:00

abstract：:The complete amino acid sequence for human erythrocyte band 4.2 has been derived from the nucleotide sequence of a full-length 2.35-kilobase (kb) cDNA. The 2.35-kb cDNA was isolated from a human reticulocyte cDNA library made in the expression vector lambda gt11. Of the 2348 base pairs (bp), 2073 bp encode 691 amino a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Korsgren C,Lawler J,Lambert S,Speicher D,Cohen CM

更新日期：1990-01-01 00:00:00

abstract：:We previously demonstrated that the primary region of factor IX and IXa responsible for saturable specific binding to bovine aortic endothelial cells resides in residues 3-11 at the amino terminus of factor IX. We also demonstrated that mutations of lysine to alanine at residue 5, factor IX K5A, or valine to lysine at...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheung WF,van den Born J,Kühn K,Kjellén L,Hudson BG,Stafford DW

更新日期：1996-10-01 00:00:00

abstract：:Certain types of human papillomaviruses (HPVs) are etiologically linked to cervical cancer. Their transforming capacity is encoded by a polycistronic premRNA, where alternative splicing leads to the translation of functional distinct proteins such as E6, E6*, and E7. Here we show that splicing of HPV16 E6/E7 ORF casse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rosenberger S,De-Castro Arce J,Langbein L,Steenbergen RD,Rösl F

更新日期：2010-04-13 00:00:00

abstract：:RNase T2 enzymes are conserved in most eukaryotic genomes, and expression patterns and phylogenetic analyses suggest that they may carry out an important housekeeping role. However, the nature of this role has been elusive. Here we show that RNS2, an intracellular RNase T2 from Arabidopsis thaliana, is essential for n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hillwig MS,Contento AL,Meyer A,Ebany D,Bassham DC,Macintosh GC

更新日期：2011-01-18 00:00:00

abstract：:The role of intraspecific and interspecific interactions in structuring biotic communities at fine spatial scales is well documented, but the signature of species interactions at coarser spatial scales is unclear. We present evidence that species interactions may be a significant factor in mediating the regional assem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gotelli NJ,Graves GR,Rahbek C

更新日期：2010-03-16 00:00:00

abstract：:Primary prostate cancer is the most common malignancy in men but has highly variable outcomes, highlighting the need for biomarkers to determine which patients can be managed conservatively. Few large prostate oncogenome resources currently exist that combine the molecular and clinical outcome data necessary to discov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hieronymus H,Schultz N,Gopalan A,Carver BS,Chang MT,Xiao Y,Heguy A,Huberman K,Bernstein M,Assel M,Murali R,Vickers A,Scardino PT,Sander C,Reuter V,Taylor BS,Sawyers CL

更新日期：2014-07-29 00:00:00

abstract：:Structural differences between dynein and kinesin suggest a unique molecular mechanism of dynein motility. Measuring the mechanical properties of a single molecule of dynein is crucial for revealing the mechanisms underlying its movement. We measured the step size and force produced by single molecules of active cytop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toba S,Watanabe TM,Yamaguchi-Okimoto L,Toyoshima YY,Higuchi H

更新日期：2006-04-11 00:00:00

abstract：:Saccharomyces cerevisiae is an ideal host from which to obtain high levels of posttranslationally modified eukaryotic proteins for x-ray crystallography. However, extensive replacement of methionine by selenomethionine for anomalous dispersion phasing has proven intractable in yeast. We report a general method to inco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malkowski MG,Quartley E,Friedman AE,Babulski J,Kon Y,Wolfley J,Said M,Luft JR,Phizicky EM,DeTitta GT,Grayhack EJ

更新日期：2007-04-17 00:00:00

abstract：:Paracoccidioidomycosis, a disease caused by Paracoccidioides brasiliensis, which is endemic to Latin America, is much more common in men than women, suggesting a role for hormonal factors. We recently showed that two other yeasts possess steroid binding proteins and postulated that these receptor-like molecules repres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loose DS,Stover EP,Restrepo A,Stevens DA,Feldman D

更新日期：1983-12-01 00:00:00

abstract：:Amphiregulin (AR) and cripto are proteins that are structurally related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). AR is also functionally related to this family of growth regulatory molecules and is able to bind and activate the 170-kDa EGF receptor (EGFR). Human EGFR-3 (HER3)/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ciardiello F,Kim N,Saeki T,Dono R,Persico MG,Plowman GD,Garrigues J,Radke S,Todaro GJ,Salomon DS

更新日期：1991-09-01 00:00:00

abstract：:In higher eukaryotic cells, mitochondria are essential subcellular organelles for energy production, cell signaling, and the biosynthesis of biomolecules. The mitochondrial DNA (mtDNA) genome is indispensable for mitochondrial function because it encodes protein subunits of the electron transport chain and a full set ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu W,Boyd RM,Tree MO,Samkari F,Zhao L

更新日期：2019-09-03 00:00:00

abstract：:Zn2+ is critical for the functional and structural integrity of cells and contributes to a number of important processes including gene expression. It has been shown that NO exogenously applied via NO donors resulting in nitrosative stress leads to cytoplasmic Zn2+ release from the zinc storing protein metallothionein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spahl DU,Berendji-Grün D,Suschek CV,Kolb-Bachofen V,Kröncke KD

更新日期：2003-11-25 00:00:00

abstract：:Research on global patterns of diversity has been dominated by studies seeking explanations for the equator-to-poles decline in richness of most groups of organisms, namely the latitudinal diversity gradient. A problem with this gradient is that it conflates two key explanations, namely biome stability (age and area) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colville JF,Beale CM,Forest F,Altwegg R,Huntley B,Cowling RM

更新日期：2020-08-18 00:00:00

abstract：:The basolateral membranes of Aplysia californica foregut absorptive cells contain both Cl(-)-stimulated ATPase and ATP-dependent Cl- transport activities, and each was inhibited by orthovanadate. Both of these orthovanadate-sensitive activities were reconstituted into proteoliposomes. The reaction sequence kinetics we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerencser GA,Zelezna B

更新日期：1993-09-01 00:00:00