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Insulin receptor synthesis and turnover in differentiating 3T3-L1 preadipocytes.

Abstract：

:A density-shift method is described for analyzing insulin receptor synthesis and turnover in cultured cells labeled with "heavy" amino acids (2H, 13C, and 15N). Solubilized newly synthesized heavy and old "light" receptors are separated by isopycnic banding on CsCl gradients and then quantitated. Insulin receptor synthesis and turnover were studied by this technique in 3T3-L1 preadipocytes which undergo an increase in insulin binding capacity during differentiation. The results indicate that the increase in insulin binding capacity is a consequence of new receptor synthesis, that the insulin receptor has a relatively short half-life (6.7 hr), and that an increased rate of receptor synthesis contributes to the increase of insulin receptor level during differentiation.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Reed BC,Lane MD

doi

10.1073/pnas.77.1.285

subject

Has Abstract

pub_date

1980-01-01 00:00:00

pages

285-9

issue

1

eissn

0027-8424

issn

1091-6490

journal_volume

77

pub_type

杂志文章

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