Use of site-directed mutagenesis to elucidate the role of arginine-166 in the catalytic mechanism of alkaline phosphatase.

abstract：:Recombinational repair of double-stranded DNA gaps was investigated in Ustilago maydis. The experimental system was designed for analysis of repair of an autonomously replicating plasmid containing a cloned gene disabled by an internal deletion. It was discovered that crossing over rarely accompanied gap repair. The s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferguson DO,Holloman WK

更新日期：1996-05-28 00:00:00

abstract：:Long-term plasticity can differ from short-term in recruiting the growth of new synaptic connections, a process that requires the participation of both the presynaptic and postsynaptic components of the synapse. How does information about synaptic plasticity spread from its site of origin to recruit the other componen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin I,Puthanveettil S,Udo H,Karl K,Kandel ER,Hawkins RD

更新日期：2012-06-05 00:00:00

abstract：:Down syndrome (DS) caused by trisomy of chromosome 21 is the most common genetic cause of intellectual disability. Although the prenatal diagnosis of DS has become feasible, there are no therapies available for the rescue of DS-related neurocognitive impairment. A growth inducer newly identified in our screen of neura...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakano-Kobayashi A,Awaya T,Kii I,Sumida Y,Okuno Y,Yoshida S,Sumida T,Inoue H,Hosoya T,Hagiwara M

更新日期：2017-09-19 00:00:00

abstract：:Niemann-Pick type C (NPC) disease is predominantly caused by mutations in the NPC1 protein that affect intracellular cholesterol trafficking and cause accumulation of unesterified cholesterol and other lipids in lysosomal storage organelles. We report the use of a series of small molecule histone deacetylase (HDAC) in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pipalia NH,Cosner CC,Huang A,Chatterjee A,Bourbon P,Farley N,Helquist P,Wiest O,Maxfield FR

更新日期：2011-04-05 00:00:00

abstract：:Ubiquitylation of proteins can be a signal for a variety of cellular processes beyond the classical role in proteolysis. The different signaling functions of ubiquitylation are thought to rely on ubiquitin-binding domains (UBDs). Several distinct UBD families are known, but their functions are not understood in detail...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tyrrell A,Flick K,Kleiger G,Zhang H,Deshaies RJ,Kaiser P

更新日期：2010-11-16 00:00:00

abstract：:Bacterial virulence factors are typically surface-associated or secreted molecules that in Gram-negative bacteria must cross the outer membrane (OM). Protein translocation across the bacterial OM is not well understood. To elucidate this process we studied P pilus biogenesis in Escherichia coli. We present high-resolu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thanassi DG,Saulino ET,Lombardo MJ,Roth R,Heuser J,Hultgren SJ

更新日期：1998-03-17 00:00:00

abstract：:We induced an essential fatty acid deficiency (EFAD) in human umbilical vein endothelial cells by culture in medium with 20% (vol/vol) delipidated fetal calf serum. EFAD, reflected by decreased cellular linoleic acid (18:2 omega 6) and arachidonic acid (20:4 omega 6) and emergence of the oleic acid derivative 5,8,11-e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lerner R,Lindström P,Berg A,Johansson E,Rosendahl K,Palmblad J

更新日期：1995-02-14 00:00:00

abstract：:In the northern hemisphere, there is compelling evidence for climate-related advances of spring events, but no such long-term biological time series exist for the southern hemisphere. We have studied a unique data set of dates of first arrival and laying of first eggs over a 55-year period for the entire community of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barbraud C,Weimerskirch H

更新日期：2006-04-18 00:00:00

abstract：:Genetic and biochemical studies have revealed that the 5' noncoding region of poliovirus mediates translation of the viral mRNA by an unusual mechanism involving entry of ribosomes in internal sequences of mRNA molecules. We have found that mRNAs bearing the 5' noncoding region of poliovirus were translated at an enha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hambidge SJ,Sarnow P

更新日期：1992-11-01 00:00:00

abstract：:This paper describes experiments in which cytosolic Ca2+ concentrations ([Ca2+]i) and catecholamine release were measured in two populations of chromaffin cells stimulated with a solution enriched in K+ (100 mM). Once depolarized, external Ca2+ or Ba2+ ions were offered to cells either as a single 2.5 mM step or as a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Michelena P,García-Pérez LE,Artalejo AR,García AG

更新日期：1993-04-15 00:00:00

abstract：:Antibody raised against the human erythrocyte glucose transporter identified a recombinant lambda gt11 bacteriophage in a cDNA library prepared from immunoselected polysomal RNA from adult rat brain. The cDNA predicts a 492-amino acid protein that demonstrates 97.6% identity to the human hepatoma hexose carrier. The t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Birnbaum MJ,Haspel HC,Rosen OM

更新日期：1986-08-01 00:00:00

abstract：:Poly(ADP ribose) polymerase inhibitors (PARPi) have efficacy in triple negative breast (TNBC) and ovarian cancers (OCs) harboring BRCA mutations, generating homologous recombination deficiencies (HRDs). DNA methyltransferase inhibitors (DNMTi) increase PARP trapping and reprogram the DNA damage response to generate HR...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McLaughlin LJ,Stojanovic L,Kogan AA,Rutherford JL,Choi EY,Yen RC,Xia L,Zou Y,Lapidus RG,Baylin SB,Topper MJ,Rassool FV

更新日期：2020-07-28 00:00:00

abstract：:Mechanisms underlying age-related obesity and insulin resistance are generally unknown. Here, we report age-related adipose vascular changes markedly modulated fat mass, adipocyte functions, blood lipid composition, and insulin sensitivity. Notably, VEGF expression levels in various white adipose tissues (WATs) underw...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Honek J,Seki T,Iwamoto H,Fischer C,Li J,Lim S,Samani NJ,Zang J,Cao Y

更新日期：2014-10-14 00:00:00

abstract：:The completion of the human genome project and the development of high-throughput approaches herald a dramatic acceleration in the pace of biological research. One of the most compelling next steps will be learning the functional roles of all proteins. Achievement of this goal depends in part on the rapid expression a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Braun P,Hu Y,Shen B,Halleck A,Koundinya M,Harlow E,LaBaer J

更新日期：2002-03-05 00:00:00

abstract：:Myocytes in the atria contain a prohormone that gives rise to atrial natriuretic peptides (ANP), which have intrinsic hemodynamic regulatory activity. Little is known about mechanisms regulating ANP release. In rats with indwelling catheters, acute blood volume expansion with 5% (wt/vol) dextrose increases the amount ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zamir N,Haass M,Dave JR,Zukowska-Grojec Z

更新日期：1987-01-01 00:00:00

abstract：:Phycobilisomes isolated from eight different species of cyanobacteria contain in addition to the light-harvesting phycobiliproteins, a small number of colorless polypeptides with molecular weights higher than those of the chromopolypeptide subunits of the phycobiliproteins. In the phycobilisomes of the species examin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Marsac NT,Cohen-bazire G

更新日期：1977-04-01 00:00:00

abstract：:Heparin is the most widely prescribed biopharmaceutical in production globally. Its potent anticoagulant activity and safety makes it the drug of choice for preventing deep vein thrombosis and pulmonary embolism. In 2008, adulterated material was introduced into the heparin supply chain, resulting in several hundred d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weiss RJ,Spahn PN,Toledo AG,Chiang AWT,Kellman BP,Li J,Benner C,Glass CK,Gordts PLSM,Lewis NE,Esko JD

更新日期：2020-04-28 00:00:00

abstract：:The CD8 dimer interacts with the alpha 3 domain of major histocompatibility complex class I molecules through two immunoglobulin variable-like domains. In this study a crystal structure-informed mutational analysis has been performed to identify amino acids in the CD8 alpha/alpha homodimer that are likely to be involv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giblin PA,Leahy DJ,Mennone J,Kavathas PB

更新日期：1994-03-01 00:00:00

abstract：:Disruption of neurotransmitter vesicle dynamics (transport, capacity, release) has been implicated in a variety of neurodegenerative and neuropsychiatric conditions. Here, we report a novel mouse model of enhanced vesicular function via bacterial artificial chromosome (BAC)-mediated overexpression of the vesicular mon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lohr KM,Bernstein AI,Stout KA,Dunn AR,Lazo CR,Alter SP,Wang M,Li Y,Fan X,Hess EJ,Yi H,Vecchio LM,Goldstein DS,Guillot TS,Salahpour A,Miller GW

更新日期：2014-07-08 00:00:00

abstract：:Escherichia coli cells treated with toluene replicate DNA when they are provided with deoxyribonucleoside 5'-triphosphates, ATP, Mg(++), and K(+). However, when deoxycytidine 5'-triphosphate is replaced by hydroxymethyl deoxycytidine 5'-triphosphate, incorporation of nucleotides into acid-precipitable material by tolu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fleischman RA,Richardson CC

更新日期：1971-10-01 00:00:00

abstract：:gp130 is a common signal-transducing receptor component for the interleukin 6 family of cytokines functioning in, for example, immune, hematopoietic, and nervous systems. In this study, to investigate the physiological functions of gp130 and to determine the pathological consequences of impaired gp130 signals, we have...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kumanogoh A,Marukawa S,Kumanogoh T,Hirota H,Yoshida K,Lee IS,Yasui T,Yoshida K,Taga T,Kishimoto T

更新日期：1997-03-18 00:00:00

abstract：:Biological soil crusts (biocrusts)—communities of mosses, lichens, cyanobacteria, and heterotrophs living at the soil surface—are fundamental components of drylands worldwide, and destruction of biocrusts dramatically alters biogeochemical processes, hydrology, surface energy balance, and vegetation cover. Although th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferrenberg S,Reed SC,Belnap J

更新日期：2015-09-29 00:00:00

abstract：:The binding of thyroid hormone to the thyroid hormone receptor (TR) mediates important physiological effects. However, the transcriptional effects of TR mediated by the thyroid response element (TRE) cannot explain many actions of thyroid hormone. We postulate that TR can initiate rapid, non-TRE-mediated effects in th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hiroi Y,Kim HH,Ying H,Furuya F,Huang Z,Simoncini T,Noma K,Ueki K,Nguyen NH,Scanlan TS,Moskowitz MA,Cheng SY,Liao JK

更新日期：2006-09-19 00:00:00

abstract：:Recent results suggested that a large subset of heat shock protein HSP-60 reactive peripheral lymphoid gamma delta T cells preexists in normal adult mice, all members of which respond to a single segment of this common HSP. However, the experimental evidence supporting this idea involved in vitro peptide responses of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fu YX,Cranfill R,Vollmer M,Van Der Zee R,O'Brien RL,Born W

更新日期：1993-01-01 00:00:00

abstract：:A discrepancy is explored regarding the spin exchange integral estimated from magnetic field effects on recombination of the bacteriochlorophyll dimer cation (Bchl)(2)+ and the bacteriopheophytin anion Bph(-) and the measured electron transfer rate between Bph and electronically excited (Bchl)(2). In one explanation c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haberkorn R,Michel-Beyerle ME,Marcus RA

更新日期：1979-09-01 00:00:00

abstract：:The reactivities of N-iodosuccinimide and iodine with various goitrogenic compounds were studied with dimethylsulfoxide through which nitrogen had been bubbled as a solvent. Although iodine does not react with all of the compounds, N-iodosuccinimide does react with all of them. N-Iodosuccinimide may, therefore, serve ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jirousek L,Soodak M

更新日期：1973-05-01 00:00:00

abstract：:In 1950, John Nash contributed a remarkable one-page PNAS article that defined and characterized a notion of equilibrium for n- person games. This notion, now called the "Nash equilibrium," has been widely applied and adapted in economics and other behavioral sciences. Indeed, game theory, with the Nash equilibrium as...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holt CA,Roth AE

更新日期：2004-03-23 00:00:00

abstract：:Increases in cytoplasmic free calcium ([Ca2+]i) can be induced in resting B cells either by a low molecular weight (12-kDa) B-cell growth factor (LMW-BCGF) or by crosslinking the B-cell antigen CD19 with monoclonal antibody (mAb). LMW-BCGF causes a slow [Ca2+]i increase in peripheral blood and tonsillar B cells but ha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ledbetter JA,Rabinovitch PS,June CH,Song CW,Clark EA,Uckun FM

更新日期：1988-03-01 00:00:00

abstract：:alpha-Isopropylmalate synthase [3-hydroxy-4-methyl-3-carboxyvalerate 2-oxo-3-methylbutyrate-lyase (CoA-acetylating); EC 4.1.3.12], the enzyme catalyzing the first committed step in leucine biosynthesis, and homocitrate synthase [3-hydroxy-3-carboxyadipate 2-oxoglutarate-lyase (CoA-acetylating); EC 4.1.3.21], the first...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tracy JW,Kohlhaw GB

更新日期：1975-05-01 00:00:00

abstract：:An investigation by paramagnetic resonance spectroscopy of the photoreduction of ferredoxin, oxygenically by water and anoxygenically by a direct electron donor to photosystem I, led to the unexpected findings that different reductive mechanisms may be involved. Ferredoxin photoreduced by water was not reoxidized in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arnon DI,Tsujimoto HY,Tang GM

更新日期：1980-05-01 00:00:00