Abstract:
:Structural differences between dynein and kinesin suggest a unique molecular mechanism of dynein motility. Measuring the mechanical properties of a single molecule of dynein is crucial for revealing the mechanisms underlying its movement. We measured the step size and force produced by single molecules of active cytoplasmic dynein by using an optical trap and fluorescence imaging with a high temporal resolution. The velocity of dynein movement, 800 nm/s, is consistent with that reported in cells. The maximum force of 7-8 pN was independent of the ATP concentration and similar to that of kinesin. Dynein exhibited forward and occasional backwards steps of approximately 8 nm, independent of load. It is suggested that the large dynein heads take 16-nm steps by using an overlapping hand-over-hand mechanism.
journal_name
Proc Natl Acad Sci U S Aauthors
Toba S,Watanabe TM,Yamaguchi-Okimoto L,Toyoshima YY,Higuchi Hdoi
10.1073/pnas.0508511103keywords:
subject
Has Abstractpub_date
2006-04-11 00:00:00pages
5741-5issue
15eissn
0027-8424issn
1091-6490pii
0508511103journal_volume
103pub_type
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