Site-specifically modified oligodeoxyribonucleotides as templates for Escherichia coli DNA polymerase I.

abstract：:Glioblastoma multiforme (GBM; grade IV astrocytoma) is the most prevalent and aggressive form of primary brain cancer. A subpopulation of multipotent cells termed GBM cancer stem cells (CSCs) play a critical role in tumor initiation, tumor maintenance, metastasis, drug resistance, and recurrence following surgery. Her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lucki NC,Villa GR,Vergani N,Bollong MJ,Beyer BA,Lee JW,Anglin JL,Spangenberg SH,Chin EN,Sharma A,Johnson K,Sander PN,Gordon P,Skirboll SL,Wurdak H,Schultz PG,Mischel PS,Lairson LL

更新日期：2019-03-26 00:00:00

abstract：:Homozygous lymphoblastoid cell lines representing Dw subtypes of the DR2 serotype were studied for structural polymorphism at DR beta. These subtypes included Dw2, Dw12, and non-Dw2/non-Dw12. Analysis by two-dimensional gel electrophoresis showed that two DR beta genes were expressed in each cell line studied. One of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee BS,Rust NA,McMichael AJ,McDevitt HO

更新日期：1987-07-01 00:00:00

abstract：:We are only just beginning to catalog the vast diversity of cell types in the cerebral cortex. Such categorization is a first step toward understanding how diversification relates to function. All cortical projection neurons arise from a uniform pool of progenitor cells that lines the ventricles of the forebrain. It i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heavner WE,Ji S,Notwell JH,Dyer ES,Tseng AM,Birgmeier J,Yoo B,Bejerano G,McConnell SK

更新日期：2020-10-06 00:00:00

abstract：:A method to identify 5-methylcytosine (m5Cyt) in DNA immobilized on nitrocellulose paper by using antibody against m5Cyt raised in rabbits is described. Immobilized restriction fragments of DNA are incubated first with purified antibody against m5Cyt and then with goat anti-rabbit IgG labeled with 125I. Restriction fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sano H,Royer HD,Sager R

更新日期：1980-06-01 00:00:00

abstract：:NifEN is a key player in the biosynthesis of nitrogenase MoFe protein. It not only shares a considerable degree of sequence homology with the MoFe protein, but also contains clusters that are homologous to those found in the MoFe protein. Here we present an investigation of the catalytic activities of NifEN. Our data ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu Y,Yoshizawa JM,Fay AW,Lee CC,Wiig JA,Ribbe MW

更新日期：2009-10-06 00:00:00

abstract：:Recently, we established that satellite III (TGGAA)n tandem repeats, which occur at the centromeres of human chromosomes, pair with themselves to form an unusual "self-complementary" antiparallel duplex containing (GGA)2 motifs in which two unpaired guanines from opposite strands intercalate between sheared G.A base p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu L,Chou SH,Reid BR

更新日期：1996-10-29 00:00:00

abstract：:In a primitive chordate model of natural chimerism, one chimeric partner is often eliminated in a process of allogeneic resorption. Here, we identify the cellular framework underlying loss of tolerance to one partner within a natural Botryllus schlosseri chimera. We show that the principal cell type mediating chimeric...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Corey DM,Rosental B,Kowarsky M,Sinha R,Ishizuka KJ,Palmeri KJ,Quake SR,Voskoboynik A,Weissman IL

更新日期：2016-06-07 00:00:00

abstract：:USF is a family of transcription factors characterized by a highly conserved basic-helix-loop-helix-leucine zipper (bHLH-zip) DNA-binding domain. Two different USF genes, termed USF1 and USF2, are ubiquitously expressed in both humans and mice. The USF1 and USF2 proteins contain highly divergent transcriptional activa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luo X,Sawadogo M

更新日期：1996-02-06 00:00:00

abstract：:Electromyographic recordings from individual identified ankle muscles of the 7-day chick embryo (stage 31) were used to determine the organization of motor output at a developmental stage shortly after the onset of spontaneous motility in the leg. During spontaneous motility of the embryo, the electromyographic record...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bekoff A,Stein PS,Hamburger V

更新日期：1975-04-01 00:00:00

abstract：:Loss of heterozygosity on chromosome 16 is a common genetic alteration in human hepatocellular carcinoma (HCC). To clarify the pathogenetic significance of allele loss on chromosome 16, we performed restriction fragment length polymorphism analysis of 70 surgically resected tumors by using 15 polymorphic DNA markers f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsuda H,Zhang WD,Shimosato Y,Yokota J,Terada M,Sugimura T,Miyamura T,Hirohashi S

更新日期：1990-09-01 00:00:00

abstract：:Adenovirus (Ad) vectors have been extensively used to deliver recombinant genes to a great variety of cell types in vitro and in vivo. Ad-based vectors are available that replace the Ad early region 1 (E1) with recombinant foreign genes. The resultant E1-deleted vectors can then be propagated on 293 cells, a human emb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amalfitano A,Begy CR,Chamberlain JS

更新日期：1996-04-16 00:00:00

abstract：:Mathematical models have been developed to explore the population dynamics of viral diseases among wildlife. However, assessing the predictions stemming from these models with wildlife databases adequate in size and temporal duration is uncommon. An epizootic of raccoon rabies that began in the mid-Atlantic region of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Childs JE,Curns AT,Dey ME,Real LA,Feinstein L,Bjørnstad ON,Krebs JW

更新日期：2000-12-05 00:00:00

abstract：:We have used DNA transfection to identify several regions of the adenovirus genome needed to induce replication of the defective parvovirus, adenovirus-associated virus (AAV). Previous studies have indicated that only early adenovirus functions are needed to aid the replication of AAV. In this report, we demonstrate t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Janik JE,Huston MM,Rose JA

更新日期：1981-03-01 00:00:00

abstract：:The recent characterization of the prokaryotic Cys(2)His(2) zinc-finger domain, identified in Ros protein from Agrobacterium tumefaciens, has demonstrated that, although possessing a similar zinc coordination sphere, this domain is structurally very different from its eukaryotic counterpart. A search in the databases ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baglivo I,Russo L,Esposito S,Malgieri G,Renda M,Salluzzo A,Di Blasio B,Isernia C,Fattorusso R,Pedone PV

更新日期：2009-04-28 00:00:00

abstract：:Antigen.antibody complexes (Ag.Ab) prepared from (125)I-labeled bovine serum albumin and guinea pig anti-albumin were incubated at 37 degrees C for 30 min with normal human serum diluted optimally for binding (1:16) and then with autologous erythrocytes (RBC). After washing, RBC-bearing antigen.antibody.complement com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Medof ME,Prince GM,Mold C

更新日期：1982-08-01 00:00:00

abstract：:The plasma concentration of the major vitamin D metabolites; 25-hydroxyvitamin D, 1,25-dihydroxyvitamin D, and 24,25-dihydroxyvitamin D were measured during pregnancy and lactation in the adult female rat. The concentrations of these metabolites were also measured in rat pups during lactation and after weaning. The pl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Halloran BP,Barthell EN,DeLuca HF

更新日期：1979-11-01 00:00:00

abstract：:Genomic analyses of patients with congenital heart disease (CHD) have identified significant contribution from mutations affecting cilia genes and chromatin remodeling genes; however, the mechanism(s) connecting chromatin remodeling to CHD is unknown. Histone H2B monoubiquitination (H2Bub1) is catalyzed by the RNF20 c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robson A,Makova SZ,Barish S,Zaidi S,Mehta S,Drozd J,Jin SC,Gelb BD,Seidman CE,Chung WK,Lifton RP,Khokha MK,Brueckner M

更新日期：2019-07-09 00:00:00

abstract：:It has been reported that expression of familial amyotrophic lateral sclerosis (FALS)-associated mutant Cu/Zn superoxide dismutase-1 (SOD) induces apoptosis of neuronal cells in culture associated with an increase in reactive oxygen species. SOD recently has been shown to prevent calcineurin inactivation, initiating t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee JP,Palfrey HC,Bindokas VP,Ghadge GD,Ma L,Miller RJ,Roos RP

更新日期：1999-03-16 00:00:00

abstract：:There is a high mortality in patients with diabetes and severe pressure ulcers. For example, chronic pressure sores of the heels often lead to limb loss in diabetic patients. A major factor underlying this is reduced neovascularization caused by impaired activity of the transcription factor hypoxia inducible factor-1 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duscher D,Neofytou E,Wong VW,Maan ZN,Rennert RC,Inayathullah M,Januszyk M,Rodrigues M,Malkovskiy AV,Whitmore AJ,Walmsley GG,Galvez MG,Whittam AJ,Brownlee M,Rajadas J,Gurtner GC

更新日期：2015-01-06 00:00:00

abstract：:Bacteriophytochromes sense light in the near-infrared window, the spectral region where absorption by mammalian tissues is minimal, and their chromophore, biliverdin IXα, is naturally present in animal cells. These properties make bacteriophytochromes particularly attractive for optogenetic applications. However, the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ryu MH,Kang IH,Nelson MD,Jensen TM,Lyuksyutova AI,Siltberg-Liberles J,Raizen DM,Gomelsky M

更新日期：2014-07-15 00:00:00

abstract：:Tiazofurin (2-beta-D-ribofuranosyl-4-thiazole-carboxamide; NSC 286193), an antitumor carbon-linked nucleoside that inhibits IMP dehydrogenase (IMP:NAD+ oxidoreductase; EC 1.1.1.205) and depletes guanylate levels, can activate the erythroid differentiation program of K-562 human leukemia cells. Tiazofurin-mediated cell...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olah E,Natsumeda Y,Ikegami T,Kote Z,Horanyi M,Szelenyi J,Paulik E,Kremmer T,Hollan SR,Sugar J

更新日期：1988-09-01 00:00:00

abstract：:Recombination is unknown in natural populations of Rivulus marmoratus, a selfing hermaphrodite, and genetic variation is likely due to mutation alone. DNA fingerprinting with an array of microsatellite [e.g., (CT)9] and minisatellite (e.g., the 33.15 core sequence) probes reveals very high clonal diversity within samp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Turner BJ,Elder JF Jr,Laughlin TF,Davis WP,Taylor DS

更新日期：1992-11-15 00:00:00

abstract：:Exposure of murine hepatoma (Hepa 1c1c7) cells to a variety of chemical agents known to protect animals against the neoplastic, mutagenic, and other toxic effects of chemical carcinogens results in dose- and time-dependent inductions of NAD(P)H:quinone reductase (EC 1.6.99.2). This enzyme protects against quinone toxi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Long MJ,Prochaska HJ,Talalay P

更新日期：1986-02-01 00:00:00

abstract：:Nucleotide excision repair (NER) prevents skin cancer by eliminating highly genotoxic cyclobutane pyrimidine dimers (CPDs) induced in DNA by the UVB component of sunlight. NER consists of two distinct but overlapping subpathways, i.e., global NER, which removes CPD from the genome overall, and transcription-coupled NE...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mathonnet G,Leger C,Desnoyers J,Drouin R,Therrien JP,Drobetsky EA

更新日期：2003-06-10 00:00:00

abstract：:Phosphorylation of the regulatory (R) domain initiates cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel activity. To discover how the function of this domain is determined by its structure, we produced an R domain protein (R8) that spanned residues 708-831 of CFTR. Phosphorylated, but not unpho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ostedgaard LS,Baldursson O,Vermeer DW,Welsh MJ,Robertson AD

更新日期：2000-05-09 00:00:00

abstract：:The caspases are cysteine proteases that have been implicated in the execution of programmed cell death in organisms ranging from nematodes to humans. Many members of the Bcl-2 family, including Bcl-XL, are potent inhibitors of programmed cell death and inhibit activation of caspases in cells. Here, we report a direct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clem RJ,Cheng EH,Karp CL,Kirsch DG,Ueno K,Takahashi A,Kastan MB,Griffin DE,Earnshaw WC,Veliuona MA,Hardwick JM

更新日期：1998-01-20 00:00:00

abstract：:Measurements of auditory sensitivity in terms of the cochlear potentials were made in young specimens of three species of crocodilians, Caiman crocodilus and Alligator mississipiensis of the subfamily Alligatorinae and Crocodylus acutus of the subfamily Crocodylinae. These species show considerable similarity in their...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wever EG

更新日期：1971-07-01 00:00:00

abstract：:To test the hypothesis that the pubertal increase in luteinizing hormone-releasing hormone (LHRH) release is withheld by a dominant inhibitory neuronal system, the role of gamma-aminobutyric acid (GABA), a known inhibitory neurotransmitter, in the control of LHRH release was examined in conscious female monkeys at the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitsushima D,Hei DL,Terasawa E

更新日期：1994-01-04 00:00:00

abstract：:Prolyl hydroxylase and other enzymes of collagen synthesis have been found in cloned cells, including epithelium, melanoma, liver, HeLa, pituitary, kidney, and neuroblastoma. The significance of collagen biosynthesis in nonfibroblastic cells lines is discussed. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Langness U,Udenfriend S

更新日期：1974-01-01 00:00:00

abstract：:Solid-state cross-polarization/magic-angle sample-spinning (13)C NMR spectra have been recorded on chlorophyll a-water aggregates, methyl pyrochlorophyllide a, and methyl pyropheophorbide a (derivatives that lack a phytyl chain). Spectra have also been collected under a decoupling regime in which resonances of certain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown CE,Spencer RB,Burger VT,Katz JJ

更新日期：1984-01-01 00:00:00