A functional R domain from cystic fibrosis transmembrane conductance regulator is predominantly unstructured in solution.

abstract：:Ig class-switch recombination (CSR) is a region-specific process that exchanges the constant Ig heavy-chain region and thus modifies an antibody's effector function. DNA lesions in switch (S) regions are induced by activation-induced cytidine deaminase (AID) and uracil-DNA glycosylase 2 (UNG2), subsequently processed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kracker S,Imai K,Gardès P,Ochs HD,Fischer A,Durandy AH

更新日期：2010-12-21 00:00:00

abstract：:Daphnia pulex de Geer and Daphnia magna Straus populations cannot be maintained in defined (sensu stricto) media containing less than 0.1 part per billion (ppb) of selenium. A concentration of 1 ppb is sufficient to satisfy minimal needs in otherwise sufficient media. In the first generation with no selenium added to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keating KI,Dagbusan BC

更新日期：1984-06-01 00:00:00

abstract：:The kinetics of the reduction of horseheart ferricytochrome c by sodium dithionite (phosphate buffer-sodium chloride; pH 6.5, mu = 1.0, 25 degrees ) features two reaction pathways; one with the rate constant k(3) = 1.17 x 10(4) M(-1) sec(-1), the other with the rate constant k(1)k(2)/k(-1) = 6.0 x 10(4) M(-1) sec(-1)....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Creutz C,Sutin N

更新日期：1973-06-01 00:00:00

abstract：:Preclinical studies indicate that (2R,6R)-hydroxynorketamine (HNK) is a putative fast-acting antidepressant candidate. Although inhibition of NMDA-type glutamate receptors (NMDARs) is one mechanism proposed to underlie ketamine's antidepressant and adverse effects, the potency of (2R,6R)-HNK to inhibit NMDARs has not ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lumsden EW,Troppoli TA,Myers SJ,Zanos P,Aracava Y,Kehr J,Lovett J,Kim S,Wang FH,Schmidt S,Jenne CE,Yuan P,Morris PJ,Thomas CJ,Zarate CA Jr,Moaddel R,Traynelis SF,Pereira EFR,Thompson SM,Albuquerque EX,Gould TD

更新日期：2019-03-12 00:00:00

abstract：:The effect of RNA secondary structure on rho-independent and rho-dependent termination of transcription of T3 DNA by Escherichia coli RNA polymerase has been studied by incorporating, into nascent transcripts, base analogs that lead to altered base-pairing properties. A guanine --> hypoxanthine substitution, with atte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adhya S,Sarkar P,Valenzuela D,Maitra U

更新日期：1979-04-01 00:00:00

abstract：:NaeI is a remarkable type II restriction endonuclease. It must bind two recognition sequences to cleave DNA, forms a covalent protein-DNA intermediate, and is only 1 aa change away from topoisomerase and recombinase activity. The latter activities apparently derive from reactivation of a cryptic DNA ligase active site...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colandene JD,Topal MD

更新日期：1998-03-31 00:00:00

abstract：:Myosins play countless critical roles in the cell, each requiring it to be activated at a specific location and time. To control myosin VI with this specificity, we created an optogenetic tool for activating myosin VI by fusing the light-sensitive Avena sativa phototropin1 LOV2 domain to a peptide from Dab2 (LOVDab), ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： French AR,Sosnick TR,Rock RS

更新日期：2017-02-28 00:00:00

abstract：:Stimulation of resting CD4(+) T lymphocytes leads to rapid proliferation and differentiation into effector (Teff) or inducible regulatory (Treg) subsets with specific functions to promote or suppress immunity. Importantly, Teff and Treg use distinct metabolic programs to support subset specification, survival, and fun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Michalek RD,Gerriets VA,Nichols AG,Inoue M,Kazmin D,Chang CY,Dwyer MA,Nelson ER,Pollizzi KN,Ilkayeva O,Giguere V,Zuercher WJ,Powell JD,Shinohara ML,McDonnell DP,Rathmell JC

更新日期：2011-11-08 00:00:00

abstract：:Immunoglobulin- or multiple myeloma-associated amyloidosis has been distinguished by the tissue deposition of Congophilic, fibrillar protein consisting of light chains or light-chain fragments (AL amyloidosis). We now report the isolation and characterization of another form of immunoglobulin-associated amyloid obtain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eulitz M,Weiss DT,Solomon A

更新日期：1990-09-01 00:00:00

abstract：:The SCF ubiquitin ligase complex of budding yeast triggers DNA replication by catalyzing ubiquitination of the S phase cyclin-dependent kinase inhibitor SIC1. SCF is composed of three proteins-ySKP1, CDC53 (Cullin), and the F-box protein CDC4-that are conserved from yeast to humans. As part of an effort to identify co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lyapina SA,Correll CC,Kipreos ET,Deshaies RJ

更新日期：1998-06-23 00:00:00

abstract：:Four synthetic peptides that together constitute the cell attachment domain of fibronectin [Pierschbacher, M.D., Ruoslahti, E., Sundelin, J., Lind, P. & Peterson, P. (1982) J. Biol. Chem. 257, 9593-9597] were constructed and tested for their ability to induce cell attachment and spreading. One of these peptides, consi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pierschbacher M,Hayman EG,Ruoslahti E

更新日期：1983-03-01 00:00:00

abstract：:Protein aggregation is a complex physiological process, primarily determined by stress-related factors revealing the hidden aggregation propensity of proteins that otherwise are fully soluble. Here we report a mechanism by which glycolytic glyceraldehyde-3-phosphate dehydrogenase of Arabidopsis thaliana (AtGAPC1) is p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zaffagnini M,Marchand CH,Malferrari M,Murail S,Bonacchi S,Genovese D,Montalti M,Venturoli G,Falini G,Baaden M,Lemaire SD,Fermani S,Trost P

更新日期：2019-12-17 00:00:00

abstract：:The effect of phagocytosis on lectin binding to plasma membranes of polymorphonuclear leukocytes was examined. The specific activities of binding sites of concanavalin A and Ricinus communis agglutinin (defined as mug of lectin bound per 100 mug of membrane protein) were measured on isolated membranes; they decreased ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oliver JM,Ukena TE,Berlin RD

更新日期：1974-02-01 00:00:00

abstract：:Granulocyte-colony-stimulating factor (G-CSF) stimulates the proliferation and differentiation of cells of the neutrophil lineage by interaction with a specific receptor. Early signal transduction events following G-CSF receptor activation were studied. We detected tyrosine phosphorylation of both the G-CSF receptor a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nicholson SE,Oates AC,Harpur AG,Ziemiecki A,Wilks AF,Layton JE

更新日期：1994-04-12 00:00:00

abstract：:Two anti-tumor monoclonal antibodies, L6 (anticarcinoma) and 1F5 (anti-B lymphoma), were covalently linked to alkaline phosphatase (AP), forming conjugates that could bind to the surface of antigen-positive tumor cells. The conjugates were capable of converting a relatively noncytotoxic prodrug, etoposide phosphate (E...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Senter PD,Saulnier MG,Schreiber GJ,Hirschberg DL,Brown JP,Hellström I,Hellström KE

更新日期：1988-07-01 00:00:00

abstract：:We have developed an approach for deriving and characterizing antigen-receptor (CD3/Ti) signal-transduction mutants. This strategy combines receptor-mediated growth inhibition and fluorescence-activated cell sorting with the Ca2+-indicator indo-1. Despite the expression of structurally normal CD3/Ti complexes, one suc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldsmith MA,Weiss A

更新日期：1987-10-01 00:00:00

abstract：:A direct method for the determination of N-linked glycosylation sites in highly glycosylated proteins is described. Carcinoembryonic antigen (CEA) and a nonspecific crossreacting antigen (NCA) were chemically deglycosylated, and peptide maps were prepared by reverse-phase HPLC. The peptides were sequenced on a gas-pha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paxton RJ,Mooser G,Pande H,Lee TD,Shively JE

更新日期：1987-02-01 00:00:00

abstract：:Cognitive studies of memory processes demonstrate that memory for stimuli is a function of how they are encoded; stimuli processed semantically are better remembered than those processed in a perceptual or shallow fashion. This study investigates the neural correlates of this cognitive phenomenon. Twelve subjects perf...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kapur S,Craik FI,Tulving E,Wilson AA,Houle S,Brown GM

更新日期：1994-03-15 00:00:00

abstract：:Whereas amyloid-β (Aβ) accumulates in the brain of normal animals dosed with low levels of copper (Cu), the mechanism is not completely known. Cu could contribute to Aβ accumulation by altering its clearance and/or its production. Because Cu homeostasis is altered in transgenic mice overexpressing Aβ precursor protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singh I,Sagare AP,Coma M,Perlmutter D,Gelein R,Bell RD,Deane RJ,Zhong E,Parisi M,Ciszewski J,Kasper RT,Deane R

更新日期：2013-09-03 00:00:00

abstract：:Using a proteomics screen, we have identified the methyltransferase G9a as an interacting partner of the hematopoietic activator NF-E2. We show that G9a is recruited to the beta-globin locus in a NF-E2-dependent manner and spreads over the entire locus. While G9a is often regarded as a corepressor, knocking down this ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chaturvedi CP,Hosey AM,Palii C,Perez-Iratxeta C,Nakatani Y,Ranish JA,Dilworth FJ,Brand M

更新日期：2009-10-27 00:00:00

abstract：:Seamless and minimally invasive integration of 3D electronic circuitry within host materials could enable the development of materials systems that are self-monitoring and allow for communication with external environments. Here, we report a general strategy for preparing ordered 3D interconnected and addressable macr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu J,Xie C,Dai X,Jin L,Zhou W,Lieber CM

更新日期：2013-04-23 00:00:00

abstract：:Individuals with higher educational attainment live healthier and longer lives. However, not everyone benefits equally from higher education. In particular, the black-white gap in life expectancy is greater at higher levels of educational attainment. Furthermore, recent research suggests that disadvantaged African Ame...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gaydosh L,Schorpp KM,Chen E,Miller GE,Harris KM

更新日期：2018-01-02 00:00:00

abstract：:Dimyristoyl lecithin liposomes, containing cytochrome b5 reductase (NADH:ferricytochrome b5 oxidoreductase, EC 1.6.2.2) and varying amounts of cytochrome b5, were used to measure flavoprotein catalysis alone and catalysis requiring electron transfer between the reductase and cytochrome as a function of temperature. Wh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Strittmatter P,Rogers MJ

更新日期：1975-07-01 00:00:00

abstract：:We have carried out free energy perturbation calculations on DNA double-stranded hexanucleotides. The sequence d(CGCGCG)2 has been "mutated" into d(CGTGCG).d(CGCACG) with the oligonucleotide in the A, B, and Z structural forms, both in vacuo and in aqueous solution. In addition, model free energy calculations have bee...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dang LX,Pearlman DA,Kollman PA

更新日期：1990-06-01 00:00:00

abstract：:Trigger factor (TF), the first chaperone in eubacteria to encounter the emerging nascent chain, binds to the large ribosomal subunit in the vicinity of the protein exit tunnel opening and forms a sheltered folding space. Here, we present the 3.5-A crystal structure of the physiological complex of the large ribosomal s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baram D,Pyetan E,Sittner A,Auerbach-Nevo T,Bashan A,Yonath A

更新日期：2005-08-23 00:00:00

abstract：:In vitro studies have demonstrated that glia can express functional receptors for a variety of neurotransmitters. To determine whether similar neurotransmitter receptors are also expressed by glia in vivo, we examined the glial scar in the transected optic nerve of the albino rabbit by quantitative receptor autoradiog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mantyh PW,Johnson DJ,Boehmer CG,Catton MD,Vinters HV,Maggio JE,Too HP,Vigna SR

更新日期：1989-07-01 00:00:00

abstract：:Scattering of light from dielectric particles whose size is on the order of an optical wavelength underlies a plethora of visual phenomena in nature and is a foundation for optical coatings and paints. Tailoring the internal nanoscale geometry of such "photonic particles" allows tuning their optical scattering charact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tao G,Kaufman JJ,Shabahang S,Rezvani Naraghi R,Sukhov SV,Joannopoulos JD,Fink Y,Dogariu A,Abouraddy AF

更新日期：2016-06-21 00:00:00

abstract：:The nodZ gene, which is present in various soil bacteria such as Bradyrhizobium japonicum, Azorhizobium caulinodans, and Rhizobium loti, is involved in the addition of a fucosyl residue to the reducing N-acetylglucosamine residue of lipochitin oligosaccharide (LCO) signal molecules. Using an Escherichia coli strain th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Quinto C,Wijfjes AH,Bloemberg GV,Blok-Tip L,López-Lara IM,Lugtenberg BJ,Thomas-Oates JE,Spaink HP

更新日期：1997-04-29 00:00:00

abstract：:Protein-tyrosine kinases (PTKs) are critical enzymes for receptor-mediated signaling in lymphocytes. Because natural killer (NK) cells are large granular lymphocytes with specialized effector function, we set out to identify PTKs preferentially expressed in these cells. One such PTK was identified and molecularly clon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawamura M,McVicar DW,Johnston JA,Blake TB,Chen YQ,Lal BK,Lloyd AR,Kelvin DJ,Staples JE,Ortaldo JR

更新日期：1994-07-05 00:00:00

abstract：:Weeds, which have been the bane of agriculture since the beginning of civilization, are managed manually, mechanically, and, more recently, by chemicals. However, chemical control options are rapidly shrinking due to the recent rise in the number of herbicide-resistant weeds in crop fields, with few alternatives on th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pandeya D,López-Arredondo DL,Janga MR,Campbell LM,Estrella-Hernández P,Bagavathiannan MV,Herrera-Estrella L,Rathore KS

更新日期：2018-07-17 00:00:00