Isolation of cDNA clones specific for collagen IV and laminin from mouse teratocarcinoma cells.

abstract：:Embryos carrying null mutations of both retinoid X receptors alpha and beta (RXRalpha-/-/RXRbeta-/- mutants) were generated. These mutant embryos die between 9.5 and 10.5 days of gestation and display a wide range of abnormalities. The cause of the lethality appears to be the lack of formation of the labyrinthine zone...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wendling O,Chambon P,Mark M

更新日期：1999-01-19 00:00:00

abstract：:Treatment of small resting B cells with soluble F(ab')2 fragments of anti-IgM, an analogue of T-independent type 2 antigens, induced activation characterized by proliferation and the expression of surface CD5. In contrast, B cells induced to proliferate in response to thymus-dependent inductive signals provided by eit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wortis HH,Teutsch M,Higer M,Zheng J,Parker DC

更新日期：1995-04-11 00:00:00

abstract：:The gene that codes for Drosophila alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase EC 1.1.1.1) was identified in a bacteriophage lambda library of genomic Drosophila DNA by using ADH cDNA cloned DNA as a probe. The DNA sequence of the protein encoding region was shown to be in agreement with the amino acid seq...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benyajati C,Place AR,Powers DA,Sofer W

更新日期：1981-05-01 00:00:00

abstract：:The ten-eleven translocation (TET) family of methylcytosine dioxygenases initiates demethylation of DNA and is associated with tumorigenesis in many cancers; however, the mechanism is mostly unknown. Here we identify upstream activators and downstream effectors of TET1 in breast cancer using human breast cancer cells ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun M,Song CX,Huang H,Frankenberger CA,Sankarasharma D,Gomes S,Chen P,Chen J,Chada KK,He C,Rosner MR

更新日期：2013-06-11 00:00:00

abstract：:Biologists regularly search databases of DNA or protein sequences for evolutionary or functional relationships to a given query sequence. We describe a ranking algorithm that exploits the entire network structure of similarity relationships among proteins in a sequence database by performing a diffusion operation on a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weston J,Elisseeff A,Zhou D,Leslie CS,Noble WS

更新日期：2004-04-27 00:00:00

abstract：:When Drosophila melanogaster embryos were ligated at very early stages of nuclear multiplication (45-60 min after egg deposition) for the entire period of embryogenesis, egg fragments of variable sizes were able to continue development up to the hatching stage. These ligated embryos differentiated larval structures; a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schubiger G,Moseley RC,Wood WJ

更新日期：1977-05-01 00:00:00

abstract：:After more than a decade of experiments generating and studying the physics of quantized vortices in atomic gas Bose-Einstein condensates, research is beginning to focus on the roles of vortices in quantum turbulence, as well as other measures of quantum turbulence in atomic condensates. Such research directions have ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： White AC,Anderson BP,Bagnato VS

更新日期：2014-03-25 00:00:00

abstract：:Fluorescence photobleaching recovery and immunofluorescence methods have been used to study the lateral mobility and topographical distribution of a major cell surface glycoprotein (CSP). Both endogenous CSP and fluorescent-labeled exogenous CSP bind to the cell surface in a fibrillar pattern and are immobile on the e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schlessinger J,Barak LS,Hammes GG,Yamada KM,Pastan I,Webb WW,Elson EL

更新日期：1977-07-01 00:00:00

abstract：:Oligosaccharyltransferase transfers an oligosaccharide chain to the asparagine residues in proteins. The archaeal and eubacterial oligosaccharyltransferases are single subunit membrane enzymes, referred to as "AglB" (archaeal glycosylation B) and "PglB" (protein glycosylation B), respectively. Only one crystal structu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matsumoto S,Shimada A,Nyirenda J,Igura M,Kawano Y,Kohda D

更新日期：2013-10-29 00:00:00

abstract：:Previous studies have shown that the immunosuppressive and carcinogenic polycyclic aromatic hydrocarbon 7,12-dimethylbenz(a)anthracene (DMBA) impairs Ca(2+)-dependent transmembrane signaling in human and murine lymphocytes. The purpose of the present studies was to analyze potential mechanisms of immunosuppression by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Archuleta MM,Schieven GL,Ledbetter JA,Deanin GG,Burchiel SW

更新日期：1993-07-01 00:00:00

abstract：:The serological properties of the gag gene products p15 and p12 of N- and B-tropic viruses of C57BL mice have been examined. Although these viruses were serologically identical by competition assays for proteins gp71 and p30, they were readily distinguishable in competition assays for proteins p15 and p12. Two isolate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benade LE,Ihle JN,Declève A

更新日期：1978-09-01 00:00:00

abstract：:Painful peripheral neuropathies are precipitated by nerve injury from disease or trauma. All such injuries will be accompanied by an inflammatory reaction, a neuritis, that will mobilize the immune system. The role of the inflammation itself is difficult to determine in the presence of structural damage to the nerve. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bennett GJ

更新日期：1999-07-06 00:00:00

abstract：:In a recent article, Hunter uses the late George Varley and George Gradwell's long-term data on the winter moth (Operophtera brumata) and green tortrix (Tortrix viridana) populations to propose a method of quantifying the relative importance of top-down effects (because of natural enemies) and bottom-up effects (becau...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hassell MP,Crawley MJ,Godfray HC,Lawton JH

更新日期：1998-09-01 00:00:00

abstract：:Malignant spreading of cancer cells requires cell surface proteases that cleave the crosslinked collagenous matrix of connective tissues. From correlating the morphologically defined invasiveness of tumor cells with the presence of specific membrane-associated proteases, we have identified a malignant human melanoma c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aoyama A,Chen WT

更新日期：1990-11-01 00:00:00

abstract：:In vitro and in vivo triplet state electron paramagnetic resonance (epr) spectra of bacteriochlorophylls (Bchls) show important differences in (a) electron spin polarization (esp), and (b) zero field splitting (ZFS) parameters. The unusual esp and ZFS properties of the observed in vivo triplet state are best interpret...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thurnauer MC,Katz JJ,Norris JR

更新日期：1975-09-01 00:00:00

abstract：:The human fungal pathogen Candida albicans shows a significant lag in growth when diluted into fresh minimal medium. This lag is abolished by the addition of conditioned medium from a high-density culture. The active component of conditioned medium is tyrosol, which is released into the medium continuously during grow...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen H,Fujita M,Feng Q,Clardy J,Fink GR

更新日期：2004-04-06 00:00:00

abstract：:Rat hepatoma cells infected with mouse mammary tumor virus contain multiple forms of unintegrated viral DNA when grown in the presence of glucocorticoids. Using the DNA transfer procedure of Southern, we have prepared restriction endonuclease fragment maps of these forms of viral DNA. The maps indicate that: (i) the m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shank PR,Cohen JC,Varmus HE,Yamamoto KR,Ringold GM

更新日期：1978-05-01 00:00:00

abstract：:Previously, recombinant human respiratory syncytial virus (RSV) monoclonal antibody Fabs were generated by antigen selection from random combinatorial libraries displayed at the tip of filamentous phage. Two such Fabs, which exhibited high binding affinity for RSV F glycoprotein (a major protective antigen), were eval...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Crowe JE Jr,Murphy BR,Chanock RM,Williamson RA,Barbas CF 3rd,Burton DR

更新日期：1994-02-15 00:00:00

abstract：:RNA polymerase II lacking a C-terminal domain (CTD) was active in transcription with purified proteins from yeast but failed to support transcription in a yeast extract. CTD dependence could be reconstituted in the purified system by addition of two fractions from the extract. An inhibitory fraction abolished transcri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Y,Kornberg RD

更新日期：1994-03-15 00:00:00

abstract：:Usual human livers contain two major aldehyde dehydrogenase [(ALDH) aldehyde:NAD+ oxidoreductase] isozymes--i.e., a cytosolic ALDH1 component and a mitochondrial ALDH2 component--whereas approximately equal to 50% of Orientals are "atypical" and have only the ALDH1 isozyme and are missing the ALDH2 isozyme. We previou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yoshida A,Huang IY,Ikawa M

更新日期：1984-01-01 00:00:00

abstract：:Most natural populations are affected by seasonal changes in temperature, rainfall, or resource availability. Seasonally fluctuating selection could potentially make a large contribution to maintaining genetic polymorphism in populations. However, previous theory suggests that the conditions for multilocus polymorphis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wittmann MJ,Bergland AO,Feldman MW,Schmidt PS,Petrov DA

更新日期：2017-11-14 00:00:00

abstract：:We simulated the behavior of suspended carbon nanotube resonators over a broad range of temperatures to explore the physics of semiflexible polymers in underdamped environments. We find that thermal fluctuations induce strong coupling between resonance modes. This effect leads to spectral fluctuations that readily acc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barnard AW,Sazonova V,van der Zande AM,McEuen PL

更新日期：2012-11-20 00:00:00

abstract：:Myelinated fibers can be demonstrated in thin unstained brain sections of freshly killed uninjected animals. With this method myelinated fibers stand out as clearly as in stained Weigert sections from formalin-fixed brains. Unstained unfixed sections can be viewed under ordinary light within minutes after death of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Richter CP,Warner CL

更新日期：1974-03-01 00:00:00

abstract：:The Skp1-Cullin1 F-box protein-Fbw7 ubiquitin ligase regulates phosphorylation-dependent cyclin E degradation, and disruption of this pathway is associated with genetic instability and tumorigenesis. Fbw7 is a human tumor suppressor that is targeted for mutation in primary cancers. However, mechanisms other than mutat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Minella AC,Welcker M,Clurman BE

更新日期：2005-07-05 00:00:00

abstract：:We have isolated, cloned, and characterized two cDNAs corresponding to the mRNAs for cationic peroxidases synthesized by cultured peanut cells. The first clone was obtained from a phage lambda gt11 library screened with antibodies directed against the major secreted isozyme. Its predicted amino acid sequence, deduced ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Buffard D,Breda C,van Huystee RB,Asemota O,Pierre M,Ha DB,Esnault R

更新日期：1990-11-01 00:00:00

abstract：:Coincidence cloning allows the isolation of sequences held in common by two genomic DNA populations. Human DNA from two human-hamster hybrid cell lines was amplified by Alu-repeat primers (Alu PCR) and the products originating from the shared human chromosomal region were cloned. To achieve this, human sequences were ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aslanidis C,de Jong PJ

更新日期：1991-08-01 00:00:00

abstract：:Recombinant plasmids containing mutant or wild-type adenovirus serotype 2 EIa genes that produce the 12S mRNA alone, the 13S mRNA alone, or both mRNAs were cotransfected into HeLa cells with plasmids containing the viral EIIa or EIII transcription units. The amount of RNA produced from the EIIa and EIII promoters was ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leff T,Elkaim R,Goding CR,Jalinot P,Sassone-Corsi P,Perricaudet M,Kédinger C,Chambon P

更新日期：1984-07-01 00:00:00

abstract：:By the combined use of DNase I footprinting, electrophoretic mobility-shift assay, and methylation interference analysis, we have identified a series of sequence-specific protein-DNA interactions in the 5' flanking region of the rat osteocalcin gene. Stimulation of osteocalcin gene expression by 1,25-dihydroxyvitamin ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Markose ER,Stein JL,Stein GS,Lian JB

更新日期：1990-03-01 00:00:00

abstract：:The AAA-ATPase thyroid hormone receptor interacting protein 13 (TRIP13), jointly with the Mad2-binding protein p31(comet), promotes the inactivation of the mitotic (spindle assembly) checkpoint by disassembling the mitotic checkpoint complex (MCC). This checkpoint system ensures the accuracy of chromosome segregation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miniowitz-Shemtov S,Eytan E,Kaisari S,Sitry-Shevah D,Hershko A

更新日期：2015-09-15 00:00:00

abstract：:Two genes encoding steroid 21-hydroxylase [21-OHase; steroid 21-monooxygenase; steroid, hydrogen-donor: oxygen oxidoreductase (21-hydroxylating); EC 1.14.99.10], a cytochrome P-450 enzyme, have been located within the HLA major histocompatibility complex. Congenital adrenal hyperplasia due to 21-OHase deficiency is a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： White PC,Grossberger D,Onufer BJ,Chaplin DD,New MI,Dupont B,Strominger JL

更新日期：1985-02-01 00:00:00