Selective viral vector transduction of ErbB4 expressing cortical interneurons in vivo with a viral receptor-ligand bridge protein.

abstract：:Cells are nonequilibrium systems that exchange matter and energy with the environment to sustain their metabolic needs. The nonequilibrium nature of this system presents considerable challenges to developing a general theory describing its behavior; however, when studied at appropriate spatiotemporal scales, the behav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang AY,Marshall WF

更新日期：2019-10-22 00:00:00

abstract：:All decisions, whether they are personal, public, or business-related, are based on the decision maker's beliefs and values. Science can and should help decision makers by shaping their beliefs. Unfortunately, science is not easily accessible to decision makers, and scientists often do not understand decision makers' ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： von Winterfeldt D

更新日期：2013-08-20 00:00:00

abstract：:Infection with avian influenza A virus of the H5N1 subtype (isolates A/HK/212/03 and A/HK/213/03) was fatal to one of two members of a family in southern China in 2003. This incident was preceded by lethal outbreaks of H5N1 influenza in waterfowl, which are the natural hosts of these viruses and, therefore, normally h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guan Y,Poon LL,Cheung CY,Ellis TM,Lim W,Lipatov AS,Chan KH,Sturm-Ramirez KM,Cheung CL,Leung YH,Yuen KY,Webster RG,Peiris JS

更新日期：2004-05-25 00:00:00

abstract：:Suppressor of variegation 3-9 homolog 1 (SUV39H1), a histone methyltransferase, catalyzes histone 3 lysine 9 trimethylation and is involved in heterochromatin organization and genome stability. However, the mechanism for regulation of the enzymatic activity of SUV39H1 in cancer cells is not yet well known. In this stu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang D,Zhou J,Liu X,Lu D,Shen C,Du Y,Wei FZ,Song B,Lu X,Yu Y,Wang L,Zhao Y,Wang H,Yang Y,Akiyama Y,Zhang H,Zhu WG

更新日期：2013-04-02 00:00:00

abstract：:Here, we describe the identification and characterization of a nuclear body (matrix-associated deacetylase body) whose formation and integrity depend on deacetylase activity. Typically, there are 20-40 0.5-microM bodies per nucleus, although the size and number can vary substantially. The structure appears to contain ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Downes M,Ordentlich P,Kao HY,Alvarez JG,Evans RM

更新日期：2000-09-12 00:00:00

abstract：:Four polyimidazoles were used in the binding and cleavage studies with poly(U). The two polydisperse polyvinylimidazoles were previously described by others, while the other two new polymers of polyethyleneimines were prepared by cationic polymerization of oxazolines. The latter had imidazole units attached to each ni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng L,Abhilash KG,Breslow R

更新日期：2012-08-07 00:00:00

abstract：:The primary photochemistry of Fe-depleted and Zn-reconstituted reaction centers from Rhodopseudomonas sphaeroides R-26.1 was studied by transient absorption spectroscopy and compared with native, Fe(2+)-containing reaction centers. Excitation of metal-free reaction centers with 30-ps flashes produced the initial charg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirmaier C,Holten D,Debus RJ,Feher G,Okamura MY

更新日期：1986-09-01 00:00:00

abstract：:It recently has been shown that epithelial Na(+) channels are controlled by a receptor for intracellular Na(+), a G protein (G(o)), and a ubiquitin-protein ligase (Nedd4). Furthermore, mutations in the epithelial Na(+) channel that underlie the autosomal dominant form of hypertension known as Liddle's syndrome inhibit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishibashi H,Dinudom A,Harvey KF,Kumar S,Young JA,Cook DI

更新日期：1999-08-17 00:00:00

abstract：:Ubc13 is a ubiquitin-conjugating enzyme responsible for noncanonical ubiquitination of TNF receptor-associated factor (TRAF)-family adapter proteins involved in Toll-like receptor and TNF-family cytokine receptor signaling, which are regulators of innate immunity. Gene ablation was used to study the function of Ubc13 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fukushima T,Matsuzawa S,Kress CL,Bruey JM,Krajewska M,Lefebvre S,Zapata JM,Ronai Z,Reed JC

更新日期：2007-04-10 00:00:00

abstract：:The hereditary hearing-vision loss disease Usher syndrome (USH) is caused by defects in several proteins, most of which form an integrated protein network called Usher interactome. Harmonin/Ush1C is a master scaffold in the assembly of the Usher protein complexes, because harmonin is known to bind to every protein in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pan L,Yan J,Wu L,Zhang M

更新日期：2009-04-07 00:00:00

abstract：:Modern practice for training classification deepnets involves a terminal phase of training (TPT), which begins at the epoch where training error first vanishes. During TPT, the training error stays effectively zero, while training loss is pushed toward zero. Direct measurements of TPT, for three prototypical deepnet a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Papyan V,Han XY,Donoho DL

更新日期：2020-10-06 00:00:00

abstract：:Using defined oligomers of IgE, our group previously studied the quantitative relationship between the aggregation of the high affinity receptors for IgE (Fc epsilonRI) and the earliest signals initiated by such aggregation: the phosphorylation of tyrosines on the receptor. Notably, at certain doses of the oligomers s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Torigoe C,Goldstein B,Wofsy C,Metzger H

更新日期：1997-02-18 00:00:00

abstract：:Mosquitoes transmit devastating human diseases because they need vertebrate blood for egg development. Metabolism in female mosquitoes is tightly coupled with blood meal-mediated reproduction, which requires an extremely high level of energy consumption. Functional analysis has shown that major genes encoding for enzy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang X,Hou Y,Saha TT,Pei G,Raikhel AS,Zou Z

更新日期：2017-03-28 00:00:00

abstract：:Improper function of voltage-gated sodium channels (NaVs), obligatory membrane proteins for bioelectrical signaling, has been linked to a number of human pathologies. Small-molecule agents that target NaVs hold considerable promise for treatment of chronic disease. Absent a comprehensive understanding of channel struc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomas-Tran R,Du Bois J

更新日期：2016-05-24 00:00:00

abstract：:Cryopreservation is widely used to maintain backups of cells as it enables the semipermanent storage of cells. During the freezing process, ice crystals that are generated inside and outside the cells can lethally damage the cells. All conventional cryopreservation methods use at least one cryoprotective agent (CPA) t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akiyama Y,Shinose M,Watanabe H,Yamada S,Kanda Y

更新日期：2019-04-16 00:00:00

abstract：:Oncogenic mutations in the small GTPase KRAS are frequently found in human cancers, and, currently, there are no effective targeted therapies for these tumors. Using a combinatorial siRNA approach, we analyzed a panel of KRAS mutant colorectal and pancreatic cancer cell lines for their dependency on 28 gene nodes that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee CS,Lee LC,Yuan TL,Chakka S,Fellmann C,Lowe SW,Caplen NJ,McCormick F,Luo J

更新日期：2019-03-05 00:00:00

abstract：:Generating human single-nucleotide polymorphisms (SNPs) is no longer a rate-limiting step for genetic studies of disease. The number of SNPs in public databases already exceeds 200,000, and the total is expected to exceed 1,000,000 within a year. Rather, progress is limited by the inability to genotype large numbers o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirschhorn JN,Sklar P,Lindblad-Toh K,Lim YM,Ruiz-Gutierrez M,Bolk S,Langhorst B,Schaffner S,Winchester E,Lander ES

更新日期：2000-10-24 00:00:00

abstract：:Although they lie at the conceptual core of a wide range of scientific questions, the notions of irregular or "random" arrangement and the process of randomization itself have never been unambiguously defined. Algorithmic implementation of these concepts requires a combinatorial, rather than a probability-theoretic, f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singer BH,Pincus S

更新日期：1998-02-17 00:00:00

abstract：:Poliovirus type 1 cDNA sequences coding for viral capsid polypeptide VP1 were inserted into the beta-lactamase sequence of Escherichia coli plasmid pBR322. Resulting recombinant plasmid pSW119 expressed in Escherichia coli a VP1-beta-lactamase fusion protein that reacted with antibodies raised against poliovirus capsi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van der Werf S,Wychowski C,Bruneau P,Blondel B,Crainic R,Horodniceanu F,Girard M

更新日期：1983-08-01 00:00:00

abstract：:Ribozyme activity in vivo depends on achieving high-level expression, intracellular stability, target colocalization, and cleavage site access. At present, target site selection is problematic because of unforeseeable secondary and tertiary RNA structures that prevent cleavage. To overcome this design obstacle, we wis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warashina M,Kuwabara T,Kato Y,Sano M,Taira K

更新日期：2001-05-08 00:00:00

abstract：:Duchenne muscular dystrophy (DMD) is the most common and lethal genetic muscle disorder, caused by recessive mutations in the dystrophin gene. One of every 3,500 males suffers from DMD, yet no treatment is currently available. Genetic therapeutic approaches, using primarily myoblast transplantation and adenovirus-medi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang B,Li J,Xiao X

更新日期：2000-12-05 00:00:00

abstract：:Ammonium sulfate fractionation of crude extracts of E. coli yields a soluble enzyme fraction (about 25-fold purification) that catalyzes the conversion of phiX174 single-stranded DNA to duplex DNA. The reaction is rifampicin-resistant, requires single-stranded DNA, Mg++, deoxynucleoside triphosphates, and ATP, and is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schuster H,Mikolajczyk M,Rohrschneider J,Geschke B

更新日期：1975-10-01 00:00:00

abstract：:Despite extensive efforts to understand the monogenic contributions to perturbed glucose homeostasis, the complexity of genetic events that fractionally contribute to the spectrum of this pathology remain poorly understood. Proper maintenance of glucose homeostasis is the central feature of a constellation of comorbid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fleet T,Zhang B,Lin F,Zhu B,Dasgupta S,Stashi E,Tackett B,Thevananther S,Rajapakshe KI,Gonzales N,Dean A,Mao J,Timchenko N,Malovannaya A,Qin J,Coarfa C,DeMayo F,Dacso CC,Foulds CE,O'Malley BW,York B

更新日期：2015-11-03 00:00:00

abstract：:The core structures of many viruses move within cells by association with host cytoskeletal motor proteins; however, the mechanisms by which intracellular viral particles are transported toward sites of replication or the cell periphery at distinct stages of infection remain to be understood. The regulation of herpesv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith GA,Pomeranz L,Gross SP,Enquist LW

更新日期：2004-11-09 00:00:00

abstract：:The live cold-adapted (ca) A/AA/6/60 influenza vaccine is being commercially developed for worldwide use in children and is being used as a model for other live vaccines. Although it has been proven safe and immunogenic, the molecular basis of cold adaptation has never been determined. To identify sequence changes res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Herlocher ML,Maassab HF,Webster RG

更新日期：1993-07-01 00:00:00

abstract：:Cysteine site-directed mutagenesis was used to create variants of Escherichia coli ribosomal protein L7/L12 that have single cysteine substitutions, at residues 63 or 89, located in different exposed loops in the structure of the globular C-terminal domain indicated by the crystallographic structure. That structure sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oleinikov AV,Jokhadze GG,Traut RR

更新日期：1993-11-01 00:00:00

abstract：:How individuals make decisions has been a matter of long-standing debate among economists and researchers in the life sciences. In economics, subjects are viewed as optimal decision makers who maximize their overall reward income. This framework has been widely influential, but requires a complete knowledge of the rew...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kubanek J

更新日期：2017-08-08 00:00:00

abstract：:Despite early speculation to the contrary, all tropical forests studied to date display seasonal variations in the presence of new leaves, flowers, and fruits. Past studies were focused on the timing of phenological events and their cues but not on the accompanying changes in leaf area that regulate vegetation-atmosph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Myneni RB,Yang W,Nemani RR,Huete AR,Dickinson RE,Knyazikhin Y,Didan K,Fu R,Negrón Juárez RI,Saatchi SS,Hashimoto H,Ichii K,Shabanov NV,Tan B,Ratana P,Privette JL,Morisette JT,Vermote EF,Roy DP,Wolfe RE,Friedl MA,

更新日期：2007-03-20 00:00:00

abstract：:A structural gene for the alpha-subunit of RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6) has been identified and mapped between spcA and trkA, near 64 min on the E. coli chromosome. It appears to be coordinately expressed and possibly cotranscribed with the genes for ribosomal proteins...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jaskunas SR,Burgess RR,Nomura M

更新日期：1975-12-01 00:00:00

abstract：:The cDNA sequences encoding the adult alpha- and beta-globin polypeptides of a marsupial, the native cat Dasyurus viverrinus, have been cloned and their nucleotide sequences have been determined. Using these cDNA clones we have determined the chromosomal location of the native cat alpha- and beta-globin genes by in si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wainwright B,Hope R

更新日期：1985-12-01 00:00:00