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Thymic nurse cells provide microenvironment for secondary T cell receptor α rearrangement in cortical thymocytes.

Abstract：

:Distinct subsets of thymic epithelial cells (TECs) support T-cell development and selection. Isolated TECs contain multicellular complexes that enclose many viable thymocytes. However, the functions of those TECs, termed thymic nurse cells (TNCs), are unclear and the idea that TNCs are present in vivo is questioned. Here, we show that TNCs represent a fraction of cortical (c)TECs that are defined by the expression of thymoproteasomes. Intravital imaging revealed TNCs in the thymic cortex in situ, whereas TNCs were detected neither during embryogenesis nor in the postnatal thymuses of various "positive-selector" T-cell receptor (TCR)-transgenic mice, indicating that TNCs are not essential for T-cell differentiation, including positive selection. Rather, cells within TNCs were enriched for long-lived CD4(+)CD8(+) thymocytes that underwent secondary TCR-Vα rearrangement. Thus, TNC complexes are formed in vivo by persistent cTEC-thymocyte interactions that then provide a microenvironment that optimizes T-cell selection through secondary TCR rearrangement.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Nakagawa Y,Ohigashi I,Nitta T,Sakata M,Tanaka K,Murata S,Kanagawa O,Takahama Y

doi

10.1073/pnas.1213069109

subject

Has Abstract

pub_date

2012-12-11 00:00:00

pages

20572-7

issue

50

eissn

0027-8424

issn

1091-6490

pii

1213069109

journal_volume

109

pub_type

杂志文章

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