Abstract:
:The primary structure of proteins S1, the largest protein component of the Escherichia coli ribosome, has been elucidated by determining the amino acid sequence of the protein (from E. coli MRE600) and the nucleotide sequence of the S1 gene (rpsA, of a K-12 strain). The two methods gave results in perfect agreement except of two positions where possible strain specific differences were found. Protein S1 (MRE600) is composed of 557 amino acid residues (no modified amino acids were detected) and has Mr 61,159. The DNA sequence for protein S1 (K-12) suggests 556 amino acid residues. A computer survey of the sequence revealed three regions in S1 with a high degree of internal homology. The ribosome binding domain of S1 (NH2 terminus) does not show any preponderance of basic amino acids. The two cysteine and the majority of tryptophan residues of S1 as well as two od the three homologous regions were located in its middle region which contains the nucleic acid binding domain. The pattern of degenerate codon usage in the S1 gene is nonrandom and similar to that reported for other ribosomal protein genes.
journal_name
Proc Natl Acad Sci U S Aauthors
Schnier J,Kimura M,Foulaki K,Subramanian AR,Isono K,Wittmann-Liebold Bdoi
10.1073/pnas.79.4.1008subject
Has Abstractpub_date
1982-02-01 00:00:00pages
1008-11issue
4eissn
0027-8424issn
1091-6490journal_volume
79pub_type
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