Site-specific protein modifications through pyrroline-carboxy-lysine residues.

Abstract:

:Pyrroline-carboxy-lysine (Pcl) is a demethylated form of pyrrolysine that is generated by the pyrrolysine biosynthetic enzymes when the growth media is supplemented with D-ornithine. Pcl is readily incorporated by the unmodified pyrrolysyl-tRNA/tRNA synthetase pair into proteins expressed in Escherichia coli and in mammalian cells. Here, we describe a broadly applicable conjugation chemistry that is specific for Pcl and orthogonal to all other reactive groups on proteins. The reaction of Pcl with 2-amino-benzaldehyde or 2-amino-acetophenone reagents proceeds to near completion at neutral pH with high efficiency. We illustrate the versatility of the chemistry by conjugating Pcl proteins with poly(ethylene glycol)s, peptides, oligosaccharides, oligonucleotides, fluorescence, and biotin labels and other small molecules. Because Pcl is genetically encoded by TAG codons, this conjugation chemistry enables enhancements of the pharmacology and functionality of proteins through site-specific conjugation.

authors

Ou W,Uno T,Chiu HP,Grünewald J,Cellitti SE,Crossgrove T,Hao X,Fan Q,Quinn LL,Patterson P,Okach L,Jones DH,Lesley SA,Brock A,Geierstanger BH

doi

10.1073/pnas.1105197108

subject

Has Abstract

pub_date

2011-06-28 00:00:00

pages

10437-42

issue

26

eissn

0027-8424

issn

1091-6490

journal_volume

108

pub_type

杂志文章
  • Scaling identity connects human mobility and social interactions.

    abstract::Massive datasets that capture human movements and social interactions have catalyzed rapid advances in our quantitative understanding of human behavior during the past years. One important aspect affecting both areas is the critical role space plays. Indeed, growing evidence suggests both our movements and communicati...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1525443113

    authors: Deville P,Song C,Eagle N,Blondel VD,Barabási AL,Wang D

    更新日期:2016-06-28 00:00:00

  • Brain renin-angiotensin system blockade by systemically active aminopeptidase A inhibitors: a potential treatment of salt-dependent hypertension.

    abstract::The hyperactivity of the brain renin-angiotensin system (RAS) has been implicated in the development and maintenance of hypertension in several types of experimental and genetic hypertension animal models. We previously reported that in the murine brain, aminopeptidase A (APA) is involved in the conversion of angioten...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0402312101

    authors: Fournie-Zaluski MC,Fassot C,Valentin B,Djordjijevic D,Reaux-Le Goazigo A,Corvol P,Roques BP,Llorens-Cortes C

    更新日期:2004-05-18 00:00:00

  • Targeted knockout of a chemokine-like gene increases anxiety and fear responses.

    abstract::Emotional responses, such as fear and anxiety, are fundamentally important behavioral phenomena with strong fitness components in most animal species. Anxiety-related disorders continue to represent a major unmet medical need in our society, mostly because we still do not fully understand the mechanisms of these disea...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1707663115

    authors: Choi JH,Jeong YM,Kim S,Lee B,Ariyasiri K,Kim HT,Jung SH,Hwang KS,Choi TI,Park CO,Huh WK,Carl M,Rosenfeld JA,Raskin S,Ma A,Gecz J,Kim HG,Kim JS,Shin HC,Park DS,Gerlai R,Jamieson BB,Kim JS,Iremonger KJ,Lee S

    更新日期:2018-01-30 00:00:00

  • Encoding and storage of spatial information in the retrosplenial cortex.

    abstract::The retrosplenial cortex (RSC) is part of a network of interconnected cortical, hippocampal, and thalamic structures harboring spatially modulated neurons. The RSC contains head direction cells and connects to the parahippocampal region and anterior thalamus. Manipulations of the RSC can affect spatial and contextual ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1313222111

    authors: Czajkowski R,Jayaprakash B,Wiltgen B,Rogerson T,Guzman-Karlsson MC,Barth AL,Trachtenberg JT,Silva AJ

    更新日期:2014-06-10 00:00:00

  • Long-term reconstitution of the mouse hematopoietic system by embryonic stem cell-derived fetal liver.

    abstract::Murine embryonic stem (ES) cells are permanent blastocyst-derived cell lines capable of contributing to a wide variety of tissues, including the germ line, after injection into host blastocysts. Recently, we have shown that ES cells can produce all of the cells of the developing fetus after aggregation with developmen...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.17.7514

    authors: Forrester LM,Bernstein A,Rossant J,Nagy A

    更新日期:1991-09-01 00:00:00

  • Searching for missing heritability: designing rare variant association studies.

    abstract::Genetic studies have revealed thousands of loci predisposing to hundreds of human diseases and traits, revealing important biological pathways and defining novel therapeutic hypotheses. However, the genes discovered to date typically explain less than half of the apparent heritability. Because efforts have largely foc...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1322563111

    authors: Zuk O,Schaffner SF,Samocha K,Do R,Hechter E,Kathiresan S,Daly MJ,Neale BM,Sunyaev SR,Lander ES

    更新日期:2014-01-28 00:00:00

  • Human population dynamics in Europe over the Last Glacial Maximum.

    abstract::The severe cooling and the expansion of the ice sheets during the Last Glacial Maximum (LGM), 27,000-19,000 y ago (27-19 ky ago) had a major impact on plant and animal populations, including humans. Changes in human population size and range have affected our genetic evolution, and recent modeling efforts have reaffir...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1503784112

    authors: Tallavaara M,Luoto M,Korhonen N,Järvinen H,Seppä H

    更新日期:2015-07-07 00:00:00

  • Regulation of inflammatory responses by dynamic subcellular localization of RNA-binding protein Arid5a.

    abstract::Adenine-thymine (AT)-rich interactive domain 5a (Arid5a) is an RNA-binding protein found in the cytoplasm and nucleus of normally growing cells. Although Arid5a is known to play an important role in immune regulation, whether and how Arid5a subcellular localization impacts immune regulation has remained unclear. In th...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1719921115

    authors: Higa M,Oka M,Fujihara Y,Masuda K,Yoneda Y,Kishimoto T

    更新日期:2018-02-06 00:00:00

  • Revealing the structure of a catalytic combustion active-site ensemble combining uniform nanocrystal catalysts and theory insights.

    abstract::Supported metal catalysts are extensively used in industrial and environmental applications. To improve their performance, it is crucial to identify the most active sites. This identification is, however, made challenging by the presence of a large number of potential surface structures that complicate such an assignm...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2002342117

    authors: Yang AC,Choksi T,Streibel V,Aljama H,Wrasman CJ,Roling LT,Goodman ED,Thomas D,Bare SR,Sánchez-Carrera RS,Schäfer A,Li Y,Abild-Pedersen F,Cargnello M

    更新日期:2020-06-30 00:00:00

  • Evolution of olfactory receptor in oriental fruit fly Dacus dorsalis.

    abstract::Male oriental fruit flies (Dacus dorsalis) from colonies in Taiwan and Hawaii were evaluated for limit of response to various analogues of methyl eugenol. The results are interpreted in terms of the geometry and allosteric requirements of the antennal receptor that triggers the characteristic methyl eugenol reflex. Th...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.4.1561

    authors: Metcalf RL,Metcalf ER,Mitchell WC,Lee LW

    更新日期:1979-04-01 00:00:00

  • Crystal structure of mammalian selenocysteine-dependent iodothyronine deiodinase suggests a peroxiredoxin-like catalytic mechanism.

    abstract::Local levels of active thyroid hormone (3,3',5-triiodothyronine) are controlled by the action of activating and inactivating iodothyronine deiodinase enzymes. Deiodinases are selenocysteine-dependent membrane proteins catalyzing the reductive elimination of iodide from iodothyronines through a poorly understood mechan...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1323873111

    authors: Schweizer U,Schlicker C,Braun D,Köhrle J,Steegborn C

    更新日期:2014-07-22 00:00:00

  • NETSeq reveals heterogeneous nucleotide incorporation by RNA polymerase I.

    abstract::DNA sequence motifs that affect RNA polymerase transcription elongation are well studied in prokaryotic organisms and contribute directly to regulation of gene expression. Despite significant work on the regulation of eukaryotic transcription, the effect of DNA template sequence on RNA polymerase I (Pol I) transcripti...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1809421115

    authors: Clarke AM,Engel KL,Giles KE,Petit CM,Schneider DA

    更新日期:2018-12-11 00:00:00

  • Cyclic nucleotide metabolism in compensatory renal hypertrophy and neonatal kidney growth.

    abstract::Cyclic nucleotide metabolism was investigated in growing kidneys of rats during compensatory hypertrophy and during neonatal development. After unilateral nephrectomy a mild and short-lasting decrease in cyclic 3':5" adenosine monophosphate (cAMP) was observed in the hypertrophying kidney. In contrast, cyclic 3':5' gu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.73.2.524

    authors: Schlondorff D,Weber H

    更新日期:1976-02-01 00:00:00

  • The L box regulon: lysine sensing by leader RNAs of bacterial lysine biosynthesis genes.

    abstract::Expression of amino acid biosynthesis genes in bacteria is often repressed when abundant supplies of the cognate amino acid are available. Repression of the Bacillus subtilis lysC gene by lysine was previously shown to occur at the level of premature termination of transcription. In this study we show that lysine dire...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2133705100

    authors: Grundy FJ,Lehman SC,Henkin TM

    更新日期:2003-10-14 00:00:00

  • Population genetic structure of Plasmodium falciparum in the two main African vectors, Anopheles gambiae and Anopheles funestus.

    abstract::We investigated patterns of genetic diversity of Plasmodium falciparum associated with its two main African vectors: Anopheles gambiae and Anopheles funestus. We dissected 10,296 wild-caught mosquitoes from three tropical sites, two in Cameroon (Simbock and Tibati, separated by 320 km) and one in Kenya (Rota, >2,000 k...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0702715104

    authors: Annan Z,Durand P,Ayala FJ,Arnathau C,Awono-Ambene P,Simard F,Razakandrainibe FG,Koella JC,Fontenille D,Renaud F

    更新日期:2007-05-08 00:00:00

  • Recombinant thyroid hormone receptor and retinoid X receptor stimulate ligand-dependent transcription in vitro.

    abstract::The thyroid hormone and retinoid X receptors form a heterodimer with each other and mediate thyroid hormone (T3)-dependent transcription. Retinoid X receptor, in addition, forms a homodimer and mediates 9-cis-retinoic acid-dependent transcription. Here, recombinant thyroid hormone receptor and recombinant retinoid X r...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.5.1647

    authors: Lee IJ,Driggers PH,Medin JA,Nikodem VM,Ozato K

    更新日期:1994-03-01 00:00:00

  • Liver-targeted disruption of Apc in mice activates beta-catenin signaling and leads to hepatocellular carcinomas.

    abstract::Although inappropriate activation of the Wnt/beta-catenin pathway has been implicated in the development of hepatocellular carcinoma (HCC), the role of this signaling in liver carcinogenesis remains unclear. To investigate this issue, we constructed a mutant mouse strain, Apc(lox/lox), in which exon 14 of the tumor-su...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0404761101

    authors: Colnot S,Decaens T,Niwa-Kawakita M,Godard C,Hamard G,Kahn A,Giovannini M,Perret C

    更新日期:2004-12-07 00:00:00

  • A metabolic indicator of photoperiodic timing.

    abstract::Characteristics of CO2 output patterns of axenic cultures of Lemna perpusilla are being sought that would serve as biochemically definable indicators of the crucial photoperiodic events during inductive light/dark schedules, using cycles with skeleton main light periods and overall periodicities of 24 hr. As the lengt...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.73.2.501

    authors: Hillman WS

    更新日期:1976-02-01 00:00:00

  • Novel adeno-associated viruses from rhesus monkeys as vectors for human gene therapy.

    abstract::Tissues from rhesus monkeys were screened by PCR for the presence of sequences homologous to known adeno-associated virus (AAV) serotypes 1-6. DNA spanning entire rep-cap ORFs from two novel AAVs, called AAV7 and AAV8, were isolated. Sequence comparisons among these and previously described AAVs revealed the greatest ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.182412299

    authors: Gao GP,Alvira MR,Wang L,Calcedo R,Johnston J,Wilson JM

    更新日期:2002-09-03 00:00:00

  • Protein kinase C as a component of a nerve growth factor-sensitive phosphorylation system in PC12 cells.

    abstract::The treatment of PC12 cells with either nerve growth factor or phorbol 12-myristate 13-acetate caused a decrease in the phosphorylation of a soluble 100-kDa protein (Nsp100). After treatment with nerve growth factor, the activity of Ca2+/phospholipid-dependent protein kinase (protein kinase C) in the cytosol was incre...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.8.2353

    authors: Hama T,Huang KP,Guroff G

    更新日期:1986-04-01 00:00:00

  • Imaging Tetrahymena ribozyme splicing activity in single live mammalian cells.

    abstract::Tetrahymena ribozymes hold promise for repairing genetic disorders but are largely limited by their modest splicing efficiency and low production of final therapeutic proteins. Ribozyme evolution in intact living mammalian cells would greatly facilitate the discovery of new ribozyme variants with high in vivo activity...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2036553100

    authors: Hasegawa S,Jackson WC,Tsien RY,Rao J

    更新日期:2003-12-09 00:00:00

  • Humoral response to a viral glycan correlates with survival on PROSTVAC-VF.

    abstract::Therapeutic cancer vaccines can be effective for treating patients, but clinical responses vary considerably from patient to patient. Early indicators of a favorable response are crucial for making individualized treatment decisions and advancing vaccine design, but no validated biomarkers are currently available. In ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1073/pnas.1314722111

    authors: Campbell CT,Gulley JL,Oyelaran O,Hodge JW,Schlom J,Gildersleeve JC

    更新日期:2014-04-29 00:00:00

  • Perceptuo-motor, cognitive, and description-based decision-making seem equally good.

    abstract::Classical studies suggest that high-level cognitive decisions (e.g., choosing between financial options) are suboptimal. In contrast, low-level decisions (e.g., choosing where to put your feet on a rocky ridge) appear near-optimal: the perception-cognition gap. Moreover, in classical tasks, people appear to put too mu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1300239110

    authors: Jarvstad A,Hahn U,Rushton SK,Warren PA

    更新日期:2013-10-01 00:00:00

  • Autogenous suppression of an opal mutation in the gene encoding peptide chain release factor 2.

    abstract::The peptide chain release factor 2 (RF2) gene, prfB, was cloned from Salmonella typhimurium by DNA hybridization using the Escherichia coli prfB probe. The nucleotide and amino acid sequences of prfB are 87.0% and 95.6% homologous between E. coli and S. typhimurium, respectively, including an in-frame premature UGA st...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.21.8432

    authors: Kawakami K,Nakamura Y

    更新日期:1990-11-01 00:00:00

  • Endemic social diversity within natural kin groups of a cooperative bacterium.

    abstract::The spatial structure of genetic diversity underlying social variation is a critical determinant of how cooperation and conflict evolve. Here we investigated whether natural social groups of the cooperative soil bacterium Myxococcus xanthus harbor internal genetic and phenotypic variation and thus the potential for so...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1100307108

    authors: Kraemer SA,Velicer GJ

    更新日期:2011-06-28 00:00:00

  • Structural insights into the bactericidal mechanism of human peptidoglycan recognition proteins.

    abstract::Peptidoglycan recognition proteins (PGRPs) are highly conserved pattern-recognition molecules of the innate immune system that bind bacterial peptidoglycans (PGNs), which are polymers of alternating N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) cross-linked by short peptide stems. Human PRGPs are bactericid...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0701453104

    authors: Cho S,Wang Q,Swaminathan CP,Hesek D,Lee M,Boons GJ,Mobashery S,Mariuzza RA

    更新日期:2007-05-22 00:00:00

  • Overt and covert effects of endogenous and exogenous ecdysone in puparium formation of flies.

    abstract::Mature larvae of Sarcophaga argyrostoma fail to form puparia when kept in contact with water, but pupariate after having been subsequently exposed to dryness for 30 hr. They become increasingly more sensitive to injected ecdysone the longer the exposure to dry conditions. A second wet treatment halts the production of...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.67.1.331

    authors: Zdarek J,Fraenkel G

    更新日期:1970-09-01 00:00:00

  • The progesterone receptor gene maps to human chromosome band 11q13, the site of the mammary oncogene int-2.

    abstract::Progesterone is involved in the development and progression of breast cancers, and progesterone receptors (PR) are important markers of hormone dependence and disease prognosis. We have used a human PR cDNA probe, genomic DNA blotting of a series of Chinese hamster-human cell hybrids, and in situ hybridization to map ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.84.9.2877

    authors: Law ML,Kao FT,Wei Q,Hartz JA,Greene GL,Zarucki-Schulz T,Conneely OM,Jones C,Puck TT,O'Malley BW

    更新日期:1987-05-01 00:00:00

  • Long-term culture and functional characterization of follicular cells from adult normal human thyroids.

    abstract::We have obtained long-term cultures of differentiated proliferating follicular cells from normal adult human thyroid glands. In vitro growth of such human cells has been sustained by a modified F-12 medium, supplemented with bovine hypothalamus and pituitary extracts and no added thyrotropin. Cultures have been expand...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.19.9004

    authors: Curcio F,Ambesi-Impiombato FS,Perrella G,Coon HG

    更新日期:1994-09-13 00:00:00

  • Equilibrium and stability code for a diffuse plasma.

    abstract::A computer code to investigate the equilibrium and stability of a diffuse plasma in three dimensions is described that generalizes earlier work on a sharp free boundary model. Toroidal equilibria of a plasma are determined by considering paths of steepest descent associated with a new version of the variational princi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.73.4.984

    authors: Betancourt O,Garabedian P

    更新日期:1976-04-01 00:00:00