DNA relaxation dynamics as a probe for the intracellular environment.

abstract：:Protein kinases play key roles in the induction by human interferon alpha (IFN-alpha) of specific gene expression and biological activity in various human cell lines. We now report that IFN-alpha increased the 7-kb transcript for the epsilon isotype of protein kinase C (PKC-epsilon) and the cellular content of PKC-eps...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang C,Constantinescu SN,MacEwan DJ,Strulovici B,Dekker LV,Parker PJ,Pfeffer LM

更新日期：1993-08-01 00:00:00

abstract：:Peanut or groundnut (Arachis hypogaea L.), a legume of South American origin, has high seed oil content (45-56%) and is a staple crop in semiarid tropical and subtropical regions, partially because of drought tolerance conferred by its geocarpic reproductive strategy. We present a draft genome of the peanut A-genome p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Li H,Pandey MK,Yang Q,Wang X,Garg V,Li H,Chi X,Doddamani D,Hong Y,Upadhyaya H,Guo H,Khan AW,Zhu F,Zhang X,Pan L,Pierce GJ,Zhou G,Krishnamohan KA,Chen M,Zhong N,Agarwal G,Li S,Chitikineni A,Zhang GQ

更新日期：2016-06-14 00:00:00

abstract：:Selective autophagy of damaged mitochondria requires autophagy receptors optineurin (OPTN), NDP52 (CALCOCO2), TAX1BP1, and p62 (SQSTM1) linking ubiquitinated cargo to autophagic membranes. By using quantitative proteomics, we show that Tank-binding kinase 1 (TBK1) phosphorylates all four receptors on several autophagy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Richter B,Sliter DA,Herhaus L,Stolz A,Wang C,Beli P,Zaffagnini G,Wild P,Martens S,Wagner SA,Youle RJ,Dikic I

更新日期：2016-04-12 00:00:00

abstract：:To visualize histone acetylation in living cells, we developed a genetically encoded fluorescent resonance energy transfer (FRET)-based indicator. Response of the indicator reflects changes in the acetylation state of both K5 and K8 in histone H4. Using this acetylation indicator, we were able to monitor the dynamic f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sasaki K,Ito T,Nishino N,Khochbin S,Yoshida M

更新日期：2009-09-22 00:00:00

abstract：:Neonates are highly susceptible to infectious diseases and, in general, respond poorly to conventional vaccines due to immaturity of the immune system. In the present study, we hypothesized that the anti-tetanus toxoid (TT) vaccine response of neonatal mice could be enhanced by retinoic acid (RA), a bioactive retinoid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma Y,Ross AC

更新日期：2005-09-20 00:00:00

abstract：:Despite the recognized role of tumor necrosis factor (TNF) in inflammation and neuronal degeneration, anti-TNF therapeutics failed to treat neurodegenerative diseases. Animal disease models had revealed the antithetic effects of the two TNF receptors (TNFR) in the central nervous system, whereby TNFR1 has been associa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong Y,Fischer R,Naudé PJ,Maier O,Nyakas C,Duffey M,Van der Zee EA,Dekens D,Douwenga W,Herrmann A,Guenzi E,Kontermann RE,Pfizenmaier K,Eisel UL

更新日期：2016-10-25 00:00:00

abstract：:The phagocyte NADPH oxidase, dormant in resting cells, is activated during phagocytosis to produce superoxide, a precursor of microbicidal oxidants. The activated oxidase is a complex of membrane-integrated cytochrome b558, composed of 91-kDa (gp91phox) and 22-kDa (p22phox) subunits, and two cytosolic factors (p47phox...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sumimoto H,Kage Y,Nunoi H,Sasaki H,Nose T,Fukumaki Y,Ohno M,Minakami S,Takeshige K

更新日期：1994-06-07 00:00:00

abstract：:Two out of seven meningiomas tested in early cell cultures by indirect immunofluorescence staining showed simian virus 40 (SV40)-related tumor (T) antigen. In one tumor 90% of the cells were positive. An additional SV40-related antigen (U) was found in 10% of cells of a third tumor. These findings indicate that the me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weiss AF,Portmann R,Fischer H,Simon J,Zang KD

更新日期：1975-02-01 00:00:00

abstract：:The formation and migration of disconnections (line defects constrained to the grain boundary [GB] plane with both dislocation and step character) control many of the kinetic and dynamical properties of GBs and the polycrystalline materials of which they are central constituents. We demonstrate that GBs undergo a fini...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen K,Srolovitz DJ,Han J

更新日期：2020-12-29 00:00:00

abstract：:The activation of cyclin-dependent kinases (cdks) has been implicated in apoptosis induced by various stimuli. We find that the Fas-induced activation of cdc2 and cdk2 in Jurkat cells is not dependent on protein synthesis, which is shut down very early during apoptosis before caspase-3 activation. Instead, activation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou BB,Li H,Yuan J,Kirschner MW

更新日期：1998-06-09 00:00:00

abstract：:The versatility of CRISPR-Cas endonucleases as a tool for biomedical research has led to diverse applications in gene editing, programmable transcriptional control, and nucleic acid detection. Most CRISPR-Cas systems, however, suffer from off-target effects and unpredictable nonspecific binding that negatively impact ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Specht DA,Xu Y,Lambert G

更新日期：2020-05-26 00:00:00

abstract：:Cold-water conditions have excluded durophagous (skeleton-breaking) predators from the Antarctic seafloor for millions of years. Rapidly warming seas off the western Antarctic Peninsula could now facilitate their return to the continental shelf, with profound consequences for the endemic fauna. Among the likely first ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aronson RB,Smith KE,Vos SC,McClintock JB,Amsler MO,Moksnes PO,Ellis DS,Kaeli J,Singh H,Bailey JW,Schiferl JC,van Woesik R,Martin MA,Steffel BV,Deal ME,Lazarus SM,Havenhand JN,Swalethorp R,Kjellerup S,Thatje S

更新日期：2015-10-20 00:00:00

abstract：:Cisplatin is a chemotherapeutic drug used to treat a variety of cancers. Both intrinsic and acquired resistance to cisplatin, as well as toxicity, limit its effectiveness. Molecular mechanisms that underlie cisplatin resistance are poorly understood. Here we demonstrate that deletion of the yeast CTR1 gene, which enco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishida S,Lee J,Thiele DJ,Herskowitz I

更新日期：2002-10-29 00:00:00

abstract：:Morphological and genetic characters from cyprinid fishes of the genus Gila were examined to assess a hypothesized hybrid origin of Gila seminuda from the Virgin River, Arizona-Nevada-Utah. The presumed parents, Gila robusta robusta and Gila elegans, are clearly differentiated from one another based on morphology, all...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeMarais BD,Dowling TE,Douglas ME,Minckley WL,Marsh PC

更新日期：1992-04-01 00:00:00

abstract：:Mechanical stability in many biological materials is provided by the crosslinking of large structural proteins with gamma-glutamyl-epsilon-lysyl amide bonds. The three-dimensional structure of human recombinant factor XIII (EC 2.3.2.13 zymogen; protein-glutamine:amine gamma-glutamyltransferase a chain), a transglutami...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yee VC,Pedersen LC,Le Trong I,Bishop PD,Stenkamp RE,Teller DC

更新日期：1994-07-19 00:00:00

abstract：:In a previous paper we described the induction by x-irradiation or radiation-induced leukemia virus-inoculation of two classes of lymphoid T-cell neoplasms: The first class, designated T-cell lymphoblastoma (TCLB), consists of growth-factor-dependent eudiploid cells that home to the spleen and give rise to splenic tum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haas M,Altman A,Rothenberg E,Bogart MH,Jones OW

更新日期：1984-03-01 00:00:00

abstract：:It is generally thought that an effective vaccine to prevent HIV-1 infection should elicit both strong neutralizing antibody and cytotoxic T lymphocyte responses. We recently demonstrated that potent, boostable, long-lived HIV-1 envelope (Env)-specific cytotoxic T lymphocyte responses can be elicited in rhesus monkeys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Letvin NL,Montefiori DC,Yasutomi Y,Perry HC,Davies ME,Lekutis C,Alroy M,Freed DC,Lord CI,Handt LK,Liu MA,Shiver JW

更新日期：1997-08-19 00:00:00

abstract：:Phenylglyoxal is an arginine-specific reagent that inactivates creatine kinase (CK). Previous results suggest that modification of the dimeric enzyme at a single arginine residue per subunit causes complete inactivation accompanied by the loss of nucleotide binding; the actual site of modification was not identified. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wood TD,Guan Z,Borders CL Jr,Chen LH,Kenyon GL,McLafferty FW

更新日期：1998-03-31 00:00:00

abstract：:The mechanism by which antifreeze proteins (AFPs) irreversibly bind to ice has not yet been resolved. The ice-binding site of an AFP is relatively hydrophobic, but also contains many potential hydrogen bond donors/acceptors. The extent to which hydrogen bonding and the hydrophobic effect contribute to ice binding has ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garnham CP,Campbell RL,Davies PL

更新日期：2011-05-03 00:00:00

abstract：:A patient with type II diabetes associated with hyperproinsulinemia has been shown to have a point mutation in one insulin gene allele, resulting in replacement of histidine with aspartic acid at position 10 of the B-chain. To investigate the basis of the proinsulin processing defect, we introduced an identical mutati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gross DJ,Halban PA,Kahn CR,Weir GC,Villa-Komaroff L

更新日期：1989-06-01 00:00:00

abstract：:Neurofibromin gene (NF1) mutation causes neurofibromatosis type 1 (NF1), a disorder in which brain white matter deficits identified by neuroimaging are common, yet of unknown cellular etiology. In mice, Nf1 loss in adult oligodendrocytes causes myelin decompaction and increases oligodendrocyte nitric oxide (NO) levels...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Asleh J,Shofty B,Cohen N,Kavushansky A,López-Juárez A,Constantini S,Ratner N,Kahn I

更新日期：2020-09-08 00:00:00

abstract：:It is well-established morphologically that bipolar cells, the second-order neurons in the vertebrate retina, make reciprocal synapses with amacrine cells in the inner plexiform layer. However, neither the property nor the physiological function of the feedback synapse is understood. Autoradiographic and immunohistoch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tachibana M,Kaneko A

更新日期：1987-05-01 00:00:00

abstract：:Superresolution fluorescence microscopy overcomes the diffraction resolution barrier and allows the molecular intricacies of life to be revealed with greatly enhanced detail. However, many current superresolution techniques still face limitations and their implementation is typically associated with a steep learning c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dedecker P,Mo GC,Dertinger T,Zhang J

更新日期：2012-07-03 00:00:00

abstract：:Although the binding change mechanism of rotary catalysis by which F1-ATPase hydrolyzes ATP has been supported by equilibrium, kinetic, and structural observations, many questions concerning the function remain unanswered. Because of the importance of this enzyme, the search for a full understanding of its mechanism i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao YQ,Yang W,Marcus RA,Karplus M

更新日期：2003-09-30 00:00:00

abstract：:This study demonstrates the presence of an N-acetyl-D-galactosaminyltransferase in human serum and in erythrocyte membranes. This enzyme catalyzes the transfer of N-acetyl-D-galactosamine from UDP-N-acetyl-D-galactosamine to a mucin receptor and 2'-fucosyllactose that have blood group H activity and may be responsible...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim YS,Perdomo J,Bella A Jr,Nordberg J

更新日期：1971-08-01 00:00:00

abstract：:The paradigmatic example of a continuous quantum phase transition is the transverse field Ising ferromagnet. In contrast to classical critical systems, whose properties depend only on symmetry and the dimension of space, the nature of a quantum phase transition also depends on the dynamics. In the transverse field Isi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maharaj AV,Rosenberg EW,Hristov AT,Berg E,Fernandes RM,Fisher IR,Kivelson SA

更新日期：2017-12-19 00:00:00

abstract：:Human genetic studies have given evidence of familial, disease-causing mutations in the analogous amino acid residue shared by three related RNA binding proteins causative of three neurological diseases. Alteration of aspartic acid residue 290 of hnRNPA2 to valine is believed to predispose patients to multisystem prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murray DT,Zhou X,Kato M,Xiang S,Tycko R,McKnight SL

更新日期：2018-10-16 00:00:00

abstract：:Escherichia coli lipopolysaccharide (LPS)-induced nitrate biosynthesis was studied in LPS-sensitive C3H/He and LPS-resistant C3H/HeJ mice. Intraperitoneal injection of 15 micrograms of LPS led to a temporary 5- to 6-fold increase in blood nitrate concentration in the C3H/He strain. Levels of nitrate excreted in the ur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stuehr DJ,Marletta MA

更新日期：1985-11-01 00:00:00

abstract：:H-NS and Lsr2 are nucleoid-associated proteins from Gram-negative bacteria and Mycobacteria, respectively, that play an important role in the silencing of horizontally acquired foreign DNA that is more AT-rich than the resident genome. Despite the fact that Lsr2 and H-NS proteins are dissimilar in sequence and structu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon BR,Li Y,Cote A,Weirauch MT,Ding P,Hughes TR,Navarre WW,Xia B,Liu J

更新日期：2011-06-28 00:00:00

abstract：:Rapid electrical conduction in the His-Purkinje system tightly controls spatiotemporal activation of the ventricles. Although recent work has shed much light on the regulation of early specification and morphogenesis of the His-Purkinje system, less is known about how transcriptional regulation establishes impulse con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang SS,Kim KH,Rosen A,Smyth JW,Sakuma R,Delgado-Olguín P,Davis M,Chi NC,Puviindran V,Gaborit N,Sukonnik T,Wylie JN,Brand-Arzamendi K,Farman GP,Kim J,Rose RA,Marsden PA,Zhu Y,Zhou YQ,Miquerol L,Henkelman RM,Sta

更新日期：2011-08-16 00:00:00