Genetic evidence that Acanthamoeba myosin I is a true myosin.

abstract：:Double-stranded oligodeoxynucleotides that represent protein binding sites in the regulatory regions of the mouse genes encoding transthyretin (TTR) and alpha 1-antitrypsin (alpha 1-AT) bound a nuclear protein factor(s) found mainly in hepatocytes. A site in the regulatory region of the gene encoding rat serum albumin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Costa RH,Grayson DR,Xanthopoulos KG,Darnell JE Jr

更新日期：1988-06-01 00:00:00

abstract：:Neural crest stem cells arise within the central nervous system but then undergo an epithelial-to-mesenchymal transition to migrate away and contribute to the peripheral nervous system and craniofacial skeleton. Here we show that DNA methyltransferase 3B (DNMT3B) is responsible for the loss of competence of dorsal neu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu N,Strobl-Mazzulla PH,Simoes-Costa M,Sánchez-Vásquez E,Bronner ME

更新日期：2014-12-16 00:00:00

abstract：:The traditional disciplines of science are grounded in the observation and measurement of object properties. Recent advances in digital computer technology have spawned numerous computer-based imaging systems that extend the range of observation and measurement into realms that would otherwise be inaccessible. More im...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beck RN

更新日期：1993-11-01 00:00:00

abstract：:Receptors for platelet-derived growth factor (PDGF) have previously only been found on cells of mesenchymal and glial origin. This study shows PDGF receptors on an anaplastic thyroid carcinoma cell line, C 643, that was found to express thyroglobulin mRNA, confirming its origin from thyroid epithelium. Northern blot a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heldin NE,Gustavsson B,Claesson-Welsh L,Hammacher A,Mark J,Heldin CH,Westermark B

更新日期：1988-12-01 00:00:00

abstract：:Cartilage collagen fibrils, which are characterized by their thin, uniform diameters, are formed of a multicomponent system of three collagen types (II, IX, and XI) and interacting proteoglycans. We have used a genetic approach to test whether the proper assembly of this multiprotein structure was altered by overexpre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garofalo S,Metsäranta M,Ellard J,Smith C,Horton W,Vuorio E,de Crombrugghe B

更新日期：1993-05-01 00:00:00

abstract：:A key functional event in eukaryotic gene activation is the formation of dynamic protein-protein interaction networks between transcriptional activators and transcriptional coactivators. Seemingly incongruent with the tight regulation of transcription, many biochemical and biophysical studies suggest that activators u...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henley MJ,Linhares BM,Morgan BS,Cierpicki T,Fierke CA,Mapp AK

更新日期：2020-11-03 00:00:00

abstract：:In Paramecium tetraurelia, cyclic nucleotides are important physiological second messengers that could regulate dynein mechanochemistry by phosphorylation. A 29-kDa polypeptide that is phosphorylated in a cAMP- and Ca(2+)-sensitive manner in permeabilized cells and isolated axonemes is the only significant phosphoryla...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hamasaki T,Barkalow K,Richmond J,Satir P

更新日期：1991-09-15 00:00:00

abstract：:For a distribution F on R(p) and a point x in R(p) the simplicial depth D(x), which is the probability that x be inside a random simplex whose vertices are p + 1 independent observations from F, is introduced. D(x) can be viewed as a measure of depth of the point x relative to F, and its empirical version gives rise t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu RY

更新日期：1988-03-01 00:00:00

abstract：:Not having enough of what one needs has long been shown to have detrimental consequences for decision making. Recent work suggests that the experience of insufficient resources can create a "scarcity" mindset; increasing attention toward the scarce resource itself, but at the cost of attention for unrelated aspects. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huijsmans I,Ma I,Micheli L,Civai C,Stallen M,Sanfey AG

更新日期：2019-06-11 00:00:00

abstract：:Developing autoreactive B cells edit their B cell antigen receptor (BCR) in the bone marrow and are clonally deleted when they fail to reexpress an innocent BCR. Here, inducible Cre-loxP-mediated gene inversion is used to change the specificity of the BCR on mature IgM+ IgD+ B cells in vivo to address the fate of lymp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lam KP,Rajewsky K

更新日期：1998-10-27 00:00:00

abstract：:RNA movements and localization pervade biology, from embryonic development to disease. To identify RNAs at specific locations, we developed a strategy in which a uridine-adding enzyme is anchored to subcellular sites, where it directly marks RNAs with 3' terminal uridines. This localized RNA recording approach yields ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Medina-Munoz HC,Lapointe CP,Porter DF,Wickens M

更新日期：2020-09-22 00:00:00

abstract：:Reactive oxygen species (ROS) have emerged as important cellular-signaling agents for cellular survival. Herein, we demonstrate that ROS-mediated oxidation of DNA to yield 8-oxo-7,8-dihydroguanine (OG) in gene promoters is a signaling agent for gene activation. Enhanced gene expression occurs when OG is formed in guan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fleming AM,Ding Y,Burrows CJ

更新日期：2017-03-07 00:00:00

abstract：:The importance of carbon in Earth's mantle greatly exceeds its modest abundance of approximately 1,000-4,000 ppm. Carbon is a constituent of key terrestrial volatiles (CO, CO(2), CH(4)), it forms diamonds, and it may also contribute to the bulk electrical properties of the silicate Earth. In contrast to that of the ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hayden LA,Watson EB

更新日期：2008-06-24 00:00:00

abstract：:Mutations in genes encoding membrane proteins have been associated with cell death of unknown cause from invertebrate development to human degenerative diseases. A point mutation in the gene for myelin proteolipid protein (PLP) underlies oligodendrocyte death and dysmyelination in jimpy mice, an accurate model for Pel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schneider AM,Griffiths IR,Readhead C,Nave KA

更新日期：1995-05-09 00:00:00

abstract：:To study the structure and function of the gene for parathyroid hormone, we obtained recombinant plasmids containing bovine parathyroid hormone cDNA. The nucleotide sequence at the 5' terminus (relative to the sense strand) of the cDNA insert in a recombinant plasmid, pPTHi4, was different from that previously reporte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weaver CA,Gordon DF,Kemper B

更新日期：1981-07-01 00:00:00

abstract：:We have analyzed the patterns of positive and negative selection of thymocytes expressing the T cell antigen receptor (TCR) from the D10.G4.1 T cell clone. This TCR confers reactivity to several non-self MHC class II alleles with a remarkably broad range of avidities. Therefore, negative selection can be studied when ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sant'Angelo DB,Janeway CA Jr

更新日期：2002-05-14 00:00:00

abstract：:The bacteriophage P1 Cre/loxP system has become a powerful tool for in vivo manipulation of the genomes of transgenic mice. Although in vitro studies have shown that Cre can catalyze recombination between cryptic "pseudo-loxP" sites in mammalian genomes, to date there have been no reports of loxP-site infidelity in tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmidt EE,Taylor DS,Prigge JR,Barnett S,Capecchi MR

更新日期：2000-12-05 00:00:00

abstract：:Bioluminescence-based imaging of living cells has become an important tool in biological and medical research. However, many bioluminescence imaging applications are limited by the requirement of an externally provided luciferin substrate and the low bioluminescence signal which restricts the sensitivity and spatiotem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gregor C,Pape JK,Gwosch KC,Gilat T,Sahl SJ,Hell SW

更新日期：2019-12-02 00:00:00

abstract：:The 26S proteasome, a molecular machine responsible for regulated protein degradation, consists of a proteolytic core particle (20S CP) associated with 19S regulatory particles (19S RPs) subdivided into base and lid subcomplexes. The assembly of 19S RP base subcomplex is mediated by multiple dedicated chaperones. Amon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barrault MB,Richet N,Godard C,Murciano B,Le Tallec B,Rousseau E,Legrand P,Charbonnier JB,Le Du MH,Guérois R,Ochsenbein F,Peyroche A

更新日期：2012-04-24 00:00:00

abstract：:We report evidence for a developmental role of acetylcholine in axon pathfinding in the Drosophila visual system. Acetylcholine was detected on photoreceptor axons during their navigation to target sites in the brain, a time well before the formation of functional synapses. The pattern of photoreceptor axon projection...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang H,Kunes S

更新日期：2004-10-19 00:00:00

abstract：:Breast cancer is a genetically and clinically heterogeneous disease, and the contributions of different target cells and different oncogenic mutations to this heterogeneity are not well understood. Here we report that mammary tumors induced by components of the Wnt signaling pathway contain heterogeneous cell types an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Y,Welm B,Podsypanina K,Huang S,Chamorro M,Zhang X,Rowlands T,Egeblad M,Cowin P,Werb Z,Tan LK,Rosen JM,Varmus HE

更新日期：2003-12-23 00:00:00

abstract：:Two approaches have been explored for the synthesis of double-stranded DNA from single-stranded DNA template complementary to rabbit 9S globin mRNA (cDNA). (i) cDNA was elongated with dCMP or dTMP homopolymeric tracts using terminal deoxynucleotidyltransferase (EC 2.7.7.31; nucleosidetriphosphate:DNA deoxynucleotidyle...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rougeon F,Mach B

更新日期：1976-10-01 00:00:00

abstract：:Sulfoquinovose (SQ; 6-deoxy-6-sulfoglucose) is the polar head group of the plant sulfolipid SQ-diacylglycerol, and SQ comprises a major proportion of the organosulfur in nature, where it is degraded by bacteria. A first degradation pathway for SQ has been demonstrated recently, a "sulfoglycolytic" pathway, in addition...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Felux AK,Spiteller D,Klebensberger J,Schleheck D

更新日期：2015-08-04 00:00:00

abstract：:The threshold behavior of the transport properties of a random metal in the critical region near a metal-insulator transition is strongly affected by the measuring electromagnetic fields. In spite of the randomness, the electrical conductivity exhibits striking phase-coherent effects due to broken symmetry, which grea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Phillips JC

更新日期：1997-09-30 00:00:00

abstract：:When human genome was fractionated by thermal elution chromatography, repetitious DNA was found in every arbitrary temperature segment. When these repetitious DNA families were used for in situ hybridization, the following conclusions were evident: (a) Unlike the case of mouse, where essentially all centromeric hetero...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu TC,Arrighi FE,Saunders GF

更新日期：1972-06-01 00:00:00

abstract：:Previous studies have established that incubation of low density lipoprotein (LDL) with cultured endothelial cells (EC) converts it to a new form (EC-modified LDL) that is now recognized by a specific receptor on macrophages (the acetyl LDL receptor) and is taken up and degraded 3-10 times more rapidly than native LDL...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parthasarathy S,Steinbrecher UP,Barnett J,Witztum JL,Steinberg D

更新日期：1985-05-01 00:00:00

abstract：:mRNA for the large subunit (LS) of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxylase (dimerizing), EC 4.1.1.39] of Zea mays is complementary to an uninterrupted 1600-base-pair-long chloroplast DNA sequence that has been mapped precisely within the 4350-base-pair-long chloroplast DNA fragment Bam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Link G,Bogorad L

更新日期：1980-04-01 00:00:00

abstract：:Classic physiology studies dating to the 1930s demonstrate that moderate or transient glucocorticoid (GC) exposure improves muscle performance. The ergogenic properties of GCs are further evidenced by their surreptitious use as doping agents by endurance athletes and poorly understood efficacy in Duchenne muscular dys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morrison-Nozik A,Anand P,Zhu H,Duan Q,Sabeh M,Prosdocimo DA,Lemieux ME,Nordsborg N,Russell AP,MacRae CA,Gerber AN,Jain MK,Haldar SM

更新日期：2015-12-08 00:00:00

abstract：:One-half of pituitary-intact or sham-operated rats survive infection with 10(9) colony-forming units of Salmonella typhimurium, whereas rats without a pituitary gland all die within a few days. When the dose of S. typhimurium is reduced 600-fold, 15-25% of the hypophysectomized rats survive, and the survival rate is s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Edwards CK 3rd,Yunger LM,Lorence RM,Dantzer R,Kelley KW

更新日期：1991-03-15 00:00:00

abstract：:The law of the iterated logarithm provides a family of bounds all of the same order such that with probability one only finitely many partial sums of a sequence of independent and identically distributed random variables exceed some members of the family, while for others infinitely many do so. In the former case, the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Slivka J

更新日期：1969-06-01 00:00:00