cAMP-stimulated phosphorylation of an axonemal polypeptide that copurifies with the 22S dynein arm regulates microtubule translocation velocity and swimming speed in Paramecium.

abstract：:We visualized synchronous dynamic brain networks by using prewhitened (stationary) magnetoencephalography signals. Data were acquired from 248 axial gradiometers while 10 subjects fixated on a spot of light for 45 s. After fitting an autoregressive integrative moving average model and taking the residuals, all pairwis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Langheim FJ,Leuthold AC,Georgopoulos AP

更新日期：2006-01-10 00:00:00

abstract：:Genome duplication followed by massive gene loss has permanently shaped the genomes of many higher eukaryotes, particularly angiosperms. It has long been believed that a primary advantage of genome duplication is the opportunity for the evolution of genes with new functions by modification of duplicated genes. If so, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chapman BA,Bowers JE,Feltus FA,Paterson AH

更新日期：2006-02-21 00:00:00

abstract：:Type 2 diabetes mellitus (T2DM) is strongly associated with obesity in most, but not all, ethnic groups, suggesting important ethnic differences in disease susceptibility. Although it is clear that insulin resistance plays a major role in the pathogenesis of T2DM and that insulin resistance is strongly associated with...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petersen KF,Dufour S,Feng J,Befroy D,Dziura J,Dalla Man C,Cobelli C,Shulman GI

更新日期：2006-11-28 00:00:00

abstract：:The amyloid beta-protein precursor (AbetaPP) is best known as the parent molecule to the amyloid beta-peptide that accumulates in the brains of patients with Alzheimer's disease. Secreted isoforms of AbetaPP that contain the Kunitz proteinase inhibitor domain are analogous to the previously identified cell-secreted pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu F,Davis J,Miao J,Previti ML,Romanov G,Ziegler K,Van Nostrand WE

更新日期：2005-12-13 00:00:00

abstract：:Apoptosis has been shown to be a significant form of cell loss in many diseases. Detachment of photoreceptors from the retinal pigment epithelium, as seen in various retinal disorders, causes photoreceptor loss and subsequent vision decline. Although caspase-dependent apoptotic pathways are activated after retinal det...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Trichonas G,Murakami Y,Thanos A,Morizane Y,Kayama M,Debouck CM,Hisatomi T,Miller JW,Vavvas DG

更新日期：2010-12-14 00:00:00

abstract：:Cubic cage compounds composed of Co-CN-Ru linkages have been prepared which illustrate the following features: (i) new motifs for alkali metal ion complexation (i.e., cationic receptors for cations), (ii) a new family of triaza-metalloligands, and (iii) a double box-like cage. The cages are synthesized by the condensa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Contakes SM,Kuhlman ML,Ramesh M,Wilson SR,Rauchfuss TB

更新日期：2002-04-16 00:00:00

abstract：:Surface proteins of mouse thymus and spleen cells were radioiodinated with lactoperoxidase. After solubilization, the labeled proteins were precipitated by antibodies directed against mouse immunoglobulin chains; the precipitates were analyzed by radioautography after Na dodecyl sulfate-gel electrophoresis. Radioactiv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lisowska-Bernstein B,Rinuy A,Vassalli P

更新日期：1973-10-01 00:00:00

abstract：:Targeting neuroendocrine tumors expressing somatostatin receptor subtypes (sst) with radiolabeled somatostatin agonists is an established diagnostic and therapeutic approach in oncology. While agonists readily internalize into tumor cells, permitting accumulation of radioactivity, radiolabeled antagonists do not, and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ginj M,Zhang H,Waser B,Cescato R,Wild D,Wang X,Erchegyi J,Rivier J,Mäcke HR,Reubi JC

更新日期：2006-10-31 00:00:00

abstract：:Sixteen hybridomas have been identified that secrete antibodies specific to horizontal cells in the carp retina. The hybridomas have been classified into three groups based on their antibody staining patterns: group I, staining associated with all horizontal cells; group II, staining associated with the most abundant ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Young LH,Dowling JE

更新日期：1984-10-01 00:00:00

abstract：:A dinuclear nickel complex with methyl and thiolate ligands, Ni(dadt(Et))Ni(Me)(SDmp) (2), has been synthesized as a dinuclear Ni(d)-Ni(p)-site model of acetyl-CoA synthase (ACS) (dadt(Et) is N,N'-diethyl-3,7-diazanonane-1,9-dithiolate; Dmp is 2,6-dimesitylphenyl). Complex 2 was prepared via 2 methods: (i) ligand subs...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ito M,Kotera M,Matsumoto T,Tatsumi K

更新日期：2009-07-21 00:00:00

abstract：:The lacY gene of Escherichia coli was cut into two approximately equal-size fragments with Afl II and subcloned individually or together under separate lac operator/promoters in plasmid pT7-5. Under these conditions, lac permease is expressed in two portions: (i) the N-terminal portion (the N terminus, the first six p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bibi E,Kaback HR

更新日期：1990-06-01 00:00:00

abstract：:The NMR spectra at 220 MHz of lysinevasopressin and its precursors were measured in dimethyl sulfoxide, and the peaks were assigned to specific protons. Information about hydrogen bonding was obtained from the temperature coefficients of the chemical shifts. With these data, and with several chemical-shift positions a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Von Dreele PH,Brewster AI,Scheraga HA,Ferger MF,Du Vigneaud V

更新日期：1971-05-01 00:00:00

abstract：:Promoters whose temporal activity can be directly manipulated in transgenic animals provide a tool for the study of gene functions in vivo. We have evaluated a tetracycline-responsive binary system for its ability to temporally control gene expression in transgenic mice. In this system, a tetracycline-controlled trans...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Furth PA,St Onge L,Böger H,Gruss P,Gossen M,Kistner A,Bujard H,Hennighausen L

更新日期：1994-09-27 00:00:00

abstract：:Human peripheral blood lymphocytes (PBLs) were transduced with a number of recombinant retroviruses including RRz2, an LNL6-based virus with a ribozyme targeted to the human immunodeficiency virus (HIV) tat gene transcript inserted within the 3' region of the neomycin-resistance gene; RASH5, and LNHL-based virus conta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun LQ,Pyati J,Smythe J,Wang L,Macpherson J,Gerlach W,Symonds G

更新日期：1995-08-01 00:00:00

abstract：:Elucidating the mechanism of folding of polynucleotides depends on accurate estimates of free energy surfaces and a quantitative description of the kinetics of structure formation. Here, the kinetics of hairpin formation in single-stranded DNA are measured after a laser temperature jump. The kinetics are modeled as co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ansari A,Kuznetsov SV,Shen Y

更新日期：2001-07-03 00:00:00

abstract：:Human tyrosyl-DNA phosphodiesterase (TDP1) hydrolyzes the phosphodiester bond at a DNA 3'-end linked to a tyrosyl moiety and has been implicated in the repair of topoisomerase I (Top1)-DNA covalent complexes. TDP1 can also hydrolyze other 3'-end DNA alterations including 3'-phosphoglycolate and 3'-abasic sites, and ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Das BB,Dexheimer TS,Maddali K,Pommier Y

更新日期：2010-11-16 00:00:00

abstract：:Paired oxygen-isotopic analyses of abiotic carbonate and benthic-ostracode shells from lake sediments provide a continuous quantitative record of growing-season temperature for the past 2000 years in the northwestern foothills of the Alaska Range. This record reveals three time intervals of comparable warmth: anno Dom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu FS,Ito E,Brown TA,Curry BB,Engstrom DR

更新日期：2001-09-11 00:00:00

abstract：:Compared with the U.S., Japan is believed to have a collectivist culture that nurtures high trust. Results from laboratory and survey research, however, show that Americans are more likely to trust strangers than are Japanese. Why would trust be lower in a collectivist culture? We use an agent-based computational mode...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Macy MW,Sato Y

更新日期：2002-05-14 00:00:00

abstract：:Intracellular calcium-binding proteins are abundantly expressed in many neuronal populations. Previous evidence suggests that calcium-binding proteins can modulate various neuronal properties, presumably by their action as calcium buffers. The importance of calcium-binding proteins for nervous system function in an in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Airaksinen MS,Eilers J,Garaschuk O,Thoenen H,Konnerth A,Meyer M

更新日期：1997-02-18 00:00:00

abstract：:Proteins from eight eukaryotic families in the cytochrome P-450 superfamily share one region of sequence similarity. This region begins 275-310 amino acids from the amino terminus of each P-450, continues for approximately 170 residues, and ends 35-50 amino acids before the carboxyl terminus. The region can be divided...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kalb VF,Loper JC

更新日期：1988-10-01 00:00:00

abstract：:Sialic acid-containing glycosphingolipids, i.e., gangliosides, constitute a major component of neuronal cells and are thought to be essential for brain function. UDP-glucose:ceramide glucosyltransferase (Ugcg) catalyzes the initial step of glycosphingolipid (GSL) biosynthesis. To gain insight into the role of GSLs in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jennemann R,Sandhoff R,Wang S,Kiss E,Gretz N,Zuliani C,Martin-Villalba A,Jäger R,Schorle H,Kenzelmann M,Bonrouhi M,Wiegandt H,Gröne HJ

更新日期：2005-08-30 00:00:00

abstract：:Purified NADPH:cytochrome c (P-450) reductase (FpT; NADPH-ferrihemoprotein oxidoreductase, EC 1.6.2.4) can reductively activate mitomycin antibiotics through a one-electron reduction to species that alkylate DNA. To assess the involvement of FpT in the intracellular activation of the mitomycins, transfectants overexpr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Belcourt MF,Hodnick WF,Rockwell S,Sartorelli AC

更新日期：1996-01-09 00:00:00

abstract：:Ribozyme activity in vivo depends on achieving high-level expression, intracellular stability, target colocalization, and cleavage site access. At present, target site selection is problematic because of unforeseeable secondary and tertiary RNA structures that prevent cleavage. To overcome this design obstacle, we wis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warashina M,Kuwabara T,Kato Y,Sano M,Taira K

更新日期：2001-05-08 00:00:00

abstract：:In the hippocampus, a CA3 pyramidal cell forms excitatory synapses with thousands of other pyramidal cells and inhibitory interneurons. By using sequential paired recordings from three connected cells, we show that the presynaptic properties of CA3 pyramidal cell terminals, belonging to the same axon, differ according...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scanziani M,Gähwiler BH,Charpak S

更新日期：1998-09-29 00:00:00

abstract：:Using electron microscopy and fitting of crystal structures, we present the 3D reconstruction of ligand-induced dimers of intact receptor tyrosine kinase, KIT. We observe that KIT protomers form close contacts throughout the entire structure of ligand-bound receptor dimers, and that the dimeric receptors adopt multipl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Opatowsky Y,Lax I,Tomé F,Bleichert F,Unger VM,Schlessinger J

更新日期：2014-02-04 00:00:00

abstract：:Mating elicits two major changes in the reproductive behavior of many insect females. The egg-laying rate increases and the readiness to accept males (receptivity) is reduced. These postmating responses last approximately 1 week in Drosophila melanogaster. Males that do not transfer sperm but transfer seminal fluid du...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu H,Kubli E

更新日期：2003-08-19 00:00:00

abstract：:We have reported that normal human salivary gland-derived epithelial cells exclusively express keratinocyte growth factor receptor (KGFR). In the process of malignant transformation of human salivary gland tumors, KGFR gene expression disappeared concomitantly with the de novo expression of the fibroblast growth facto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Y,Wang H,Toratani S,Sato JD,Kan M,McKeehan WL,Okamoto T

更新日期：2001-09-25 00:00:00

abstract：:Previously metal-ion sites have been used as structural and functional probes in seven transmembrane receptors (7TM), but as yet all the engineered sites have been inactivating. Based on presumed agonist interaction points in transmembrane III (TM-III) and -VII of the beta(2)-adrenergic receptor, in this paper we cons...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elling CE,Thirstrup K,Holst B,Schwartz TW

更新日期：1999-10-26 00:00:00

abstract：:When infants and adults communicate, they exchange social signals of availability and communicative intention such as eye gaze. Previous research indicates that when communication is successful, close temporal dependencies arise between adult speakers' and listeners' neural activity. However, it is not known whether s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leong V,Byrne E,Clackson K,Georgieva S,Lam S,Wass S

更新日期：2017-12-12 00:00:00

abstract：:Society makes substantial investments in biomedical research, searching for ways to better human health. The product of this research is principally information published in scientific journals. Continued investment in science relies on society's confidence in the accuracy, honesty, and utility of research results. A ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bowen A,Casadevall A

更新日期：2015-09-08 00:00:00