Mild mitochondrial uncoupling impacts cellular aging in human muscles in vivo.

abstract：:Natural selection does not necessarily favor maximal reproduction because reproduction imposes fitness costs, reducing parental survival, and offspring quality. Here, we show that parents in a preindustrial population in North America incurred fitness costs from reproduction, and women incurred greater costs than men....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Penn DJ,Smith KR

更新日期：2007-01-09 00:00:00

abstract：:Transcript accumulation and translation of psbA as well as processing of the D1 precursor protein were investigated in relation to chlorophyll availability in vivo in cyanobacterial strains lacking photosystem I (PS I). The psbA transcript level was almost independent of chlorophyll availability and was approximately ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He Q,Vermaas W

更新日期：1998-05-12 00:00:00

abstract：:The 3' flanking region of the apolipoprotein B (apoB) gene contains a hypervariable region consisting of a variable number of tandemly repeated short A + T-rich DNA sequences (VNTRs). We present a general method that utilizes the polymerase chain reaction to rapidly and accurately type this and other VNTR loci. We use...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boerwinkle E,Xiong WJ,Fourest E,Chan L

更新日期：1989-01-01 00:00:00

abstract：:The c-myc protooncogene has been implicated in control of growth and differentiation of mammalian cells. For instance, growth arrest is often preceded by reduction in c-myc mRNA and gene transcription. To elucidate the mechanisms of control of c-myc gene transcription, we have begun to characterize the interaction of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duyao MP,Buckler AJ,Sonenshein GE

更新日期：1990-06-01 00:00:00

abstract：:Prophenoloxidases (PPOs) are key enzymes of the melanization reaction, which is a prominent defense mechanism of arthropods. The mosquito Aedes aegypti has ten PPO genes in the genome, four of which (PPO1, PPO3, PPO5, and PPO8) were expressed in response to microbial infection. Cactus depletion resulted in transcripti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zou Z,Shin SW,Alvarez KS,Bian G,Kokoza V,Raikhel AS

更新日期：2008-11-25 00:00:00

abstract：:Atherosclerosis is associated with alterations in nitric oxide (NO)/cGMP signaling. In early stages of the disease, inflammatory and possibly other cells produce reactive oxygen species that scavenge vasoprotective NO. In addition to the oxidative stress, expression and activity of enzymes downstream to NO formation m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Melichar VO,Behr-Roussel D,Zabel U,Uttenthal LO,Rodrigo J,Rupin A,Verbeuren TJ,Kumar H S A,Schmidt HH

更新日期：2004-11-23 00:00:00

abstract：:Deregulated production of nitric oxide (NO) has been implicated in the development of certain human diseases, including cancer. We sought to assess the damaging potential of NO produced under long-term conditions through the development of a suitable model cell culture system. In this study, we report that when murine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhuang JC,Wogan GN

更新日期：1997-10-28 00:00:00

abstract：:We have recently characterized a transposable element, ISH1, which inactivates the bacterio-opsin (BO) gene in two purple membrane-deficient (Pum-) mutants of Halobacterium halobium. Examination of nine additional Pum- mutants now shows that in all of these the BO gene has been inactivated by insertion of one of two t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DasSarma S,RajBhandary UL,Khorana HG

更新日期：1983-04-01 00:00:00

abstract：:Spleen cells derived from a rabbit hyperimmunized with a Type III pneumococcal vaccine were exposed to simian virus 40 in vitro. After 114 days, transformed cells growing on the surface of one culture dish were observed and a cell line was established. The transformed cells had a morphology characteristic of cells tra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collins JJ,Black PH,Strosberg AD,Haber E,Bloch KJ

更新日期：1974-02-01 00:00:00

abstract：:Control of iron homeostasis is essential for healthy central nervous system function: iron deficiency is associated with cognitive impairment, yet iron overload is thought to promote neurodegenerative diseases. Specific genetic markers have been previously identified that influence levels of transferrin, the protein t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jahanshad N,Kohannim O,Hibar DP,Stein JL,McMahon KL,de Zubicaray GI,Medland SE,Montgomery GW,Whitfield JB,Martin NG,Wright MJ,Toga AW,Thompson PM

更新日期：2012-04-03 00:00:00

abstract：:Inducer-mediated murine erythroleukemia cell (MELC) differentiation provides a model for examining factors determining terminal cell differentiation. The nuclear protein, p53, has been implicated as a potential determinant of cell cycle progression and cell differentiation. In this study p53 content and synthesis, dur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen DW,Real FX,DeLeo AB,Old LJ,Marks PA,Rifkind RA

更新日期：1983-10-01 00:00:00

abstract：:In vitro and in vivo triplet state electron paramagnetic resonance (epr) spectra of bacteriochlorophylls (Bchls) show important differences in (a) electron spin polarization (esp), and (b) zero field splitting (ZFS) parameters. The unusual esp and ZFS properties of the observed in vivo triplet state are best interpret...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thurnauer MC,Katz JJ,Norris JR

更新日期：1975-09-01 00:00:00

abstract：:Dictyostelium myosin II is activated by phosphorylation of its regulatory light chain by myosin light chain kinase A (MLCK-A), an unconventional MLCK that is not regulated by Ca2+/calmodulin. MLCK-A is activated by autophosphorylation of threonine-289 outside of the catalytic domain and by phosphorylation of threonine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Silveira LA,Smith JL,Tan JL,Spudich JA

更新日期：1998-10-27 00:00:00

abstract：:Reducing monosaccharides and oligosaccharides, after reductive amination, were separated and detected as their 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde (CBQCA) derivatives by capillary electrophoresis/laser-induced fluorescence. Under optimized conditions, the minimum detectable quantities for monosaccharide sol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu JP,Shirota O,Wiesler D,Novotny M

更新日期：1991-03-15 00:00:00

abstract：:Activation of phospholipase Cgamma1 (PLCgamma1) by vascular endothelial growth factor receptor-2 (VEGFR-2) in endothelial cells in part is responsible for angiogenesis in vivo. The cellular mechanisms exerting negative control over PLCgamma1 activation, however, remain unaddressed. Here by using in vitro and in vivo b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singh AJ,Meyer RD,Navruzbekov G,Shelke R,Duan L,Band H,Leeman SE,Rahimi N

更新日期：2007-03-27 00:00:00

abstract：:Receptor tyrosine kinases (RTKs) are a class of cell surface receptors that, upon ligand binding, stimulate a variety of critical cellular functions. The orphan receptor anaplastic lymphoma kinase (ALK) is one of very few RTKs that remain without a firmly established protein ligand. Here we present a novel cytokine, F...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reshetnyak AV,Murray PB,Shi X,Mo ES,Mohanty J,Tome F,Bai H,Gunel M,Lax I,Schlessinger J

更新日期：2015-12-29 00:00:00

abstract：:Although it has long been recognized that the enteric community of bacteria that inhabit the human distal intestinal track broadly impacts human health, the biochemical details that underlie these effects remain largely undefined. Here, we report a broad MS-based metabolomics study that demonstrates a surprisingly lar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wikoff WR,Anfora AT,Liu J,Schultz PG,Lesley SA,Peters EC,Siuzdak G

更新日期：2009-03-10 00:00:00

abstract：:H-2M3 encodes HMT, the major histocompatibility complex (MHC) class I heavy chain of the maternally transmitted antigen (Mta). Like classical MHC class I genes, the expression of M3 can be stimulated by gamma-interferon and its message can be detected from mid-gestational embryos (day 8) through adulthood. HMTb, a non...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang CR,Lindahl KF

更新日期：1993-04-01 00:00:00

abstract：:Several studies have suggested that introgressed Neandertal DNA was subjected to negative selection in modern humans. A striking observation in support of this is an apparent monotonic decline in Neandertal ancestry observed in modern humans in Europe over the past 45,000 years. Here, we show that this decline is an a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petr M,Pääbo S,Kelso J,Vernot B

更新日期：2019-01-29 00:00:00

abstract：:The transformation of new experiences into lasting memories is thought to be mediated by postencoding reactivation or the reexpression of activity patterns that characterize prior encoding experiences during subsequent offline periods. Although hippocampal reactivation has been well-described in the rodent, evidence f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tambini A,Davachi L

更新日期：2013-11-26 00:00:00

abstract：:Expression of the Arabidopsis TRANSPARENT TESTA 2 (TT2) MYB family transcription factor leads to massive accumulation of proanthocyanidins (PAs) in hairy roots of Medicago truncatula. Microarray analysis showed that TT2 induces genes for flavonoid/PA biosynthesis, transcription factors, and a large number of genes of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pang Y,Peel GJ,Sharma SB,Tang Y,Dixon RA

更新日期：2008-09-16 00:00:00

abstract：:The androgen receptor (AR) binds to androgen response elements and regulates target genes via a mechanism involving coregulators. Here we demonstrate that the AR can interact with the testicular orphan receptor-4 (TR4) and function as a repressor to down-regulate the TR4 target genes by preventing the TR4 binding to i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee YF,Shyr CR,Thin TH,Lin WJ,Chang C

更新日期：1999-12-21 00:00:00

abstract：:Chronic inflammation results in increased nitrogen monoxide (.NO) formation and the accumulation of nitrite (NO(2-)). Neutrophils stimulated by various inflammatory mediators release myeloperoxidase to produce the cytotoxic agent hypochlorous acid (HOCl). Exposure of chondrocytic SW1353 cells to HOCl resulted in a con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Whiteman M,Hooper DC,Scott GS,Koprowski H,Halliwell B

更新日期：2002-09-17 00:00:00

abstract：:The discovery of arbuscules in Aglaophyton major, an Early Devonian land plant, provides unequivocal evidence that mycorrhizae were established >400 million years ago. Nonseptate hyphac and arbuscules occur in a specialized meristematic region of the cortex that continually provided new cells for fungal infection. Arb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Remy W,Taylor TN,Hass H,Kerp H

更新日期：1994-12-06 00:00:00

abstract：:The epidermal growth factor (EGF) receptor gene is the cellular homolog of the avian erythroblastosis virus erbB oncogene. Control of EGF receptor expression determines cellular responsiveness to EGF and might play an important role in neoplastic development. Using RNA blot hybridization, we have found that exposure o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clark AJ,Ishii S,Richert N,Merlino GT,Pastan I

更新日期：1985-12-01 00:00:00

abstract：:Light emission produced by the reversible collapse of a cavity in a dielectric medium is given an initial, simplified treatment. The agreement between planar and spherical shapes indicates the volume nature of the effect. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwinger J

更新日期：1993-02-01 00:00:00

abstract：:Mechanisms and signals that regulate transcriptional coactivators are still largely unknown. Here we provide genetic evidence for a repressor that interacts with and regulates the nuclear receptor coactivator PGC-1. Association with the repressor requires a PGC-1 protein interface that is similar to the one used by nu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knutti D,Kressler D,Kralli A

更新日期：2001-08-14 00:00:00

abstract：:From endonuclease EcoRI partial libraries of DNAs from mouse embryo and MOPC 141, a gamma 2b-producing myeloma, clones were isolated by using a DNA fragment carrying the gamma 2b constant (C) region gene as a hybridization probe. One clone from MOPC 141 contained a heavy chain variable (V) gene and the C gamma 2b gene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maki R,Traunecker A,Sakano H,Roeder W,Tonegawa S

更新日期：1980-04-01 00:00:00

abstract：:Lakes are considered the second largest natural source of atmospheric methane (CH4). However, current estimates are still uncertain and do not account for diel variability of CH4 emissions. In this study, we performed high-resolution measurements of CH4 flux from several lakes, using an automated and sensor-based flux...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sieczko AK,Duc NT,Schenk J,Pajala G,Rudberg D,Sawakuchi HO,Bastviken D

更新日期：2020-09-01 00:00:00

abstract：:Although the first observations of the inhibitory effect of methylation on gene activity were made almost a decade ago, the mechanism by which methyl groups affect transcription is still obscure. Here we use engineered promoters methylated in vitro in transient transfections to study the mechanism by which methylation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levine A,Cantoni GL,Razin A

更新日期：1992-11-01 00:00:00