Negative selection of thymocytes expressing the D10 TCR.

Abstract:

:We have analyzed the patterns of positive and negative selection of thymocytes expressing the T cell antigen receptor (TCR) from the D10.G4.1 T cell clone. This TCR confers reactivity to several non-self MHC class II alleles with a remarkably broad range of avidities. Therefore, negative selection can be studied when induced by high-, intermediate-, or low-avidity interactions with endogenous peptide-MHC complexes, all within the same TCR transgenic system. These data directly demonstrate that MHC class II-peptide ligands that fail to activate mature T cells can promote negative selection of immature thymocytes. Additionally, we show that negative selection of thymocytes can occur at two distinct "time points" during development depending on the avidity of the TCR for the MHC-peptide complex. Finally, we show that the self-peptide repertoire plays a significant role in selection because alteration of the self-peptide repertoire by disruption of the H2-Ma gene drastically alters selection of D10 TCR-expressing thymocytes.

authors

Sant'Angelo DB,Janeway CA Jr

doi

10.1073/pnas.102182499

keywords:

subject

Has Abstract

pub_date

2002-05-14 00:00:00

pages

6931-6

issue

10

eissn

0027-8424

issn

1091-6490

pii

99/10/6931

journal_volume

99

pub_type

杂志文章
  • Growth inhibition and induction of differentiation of t(8;21) acute myeloid leukemia cells by the DNA-binding domain of PEBP2 and the AML1/MTG8(ETO)-specific antisense oligonucleotide.

    abstract::The translocation from chromosome 8 to chromosome 21, t(8;21), associated with acute myeloid leukemia results in production of an AML1/MTG8(ETO) fusion transcript. The product of the AML1 gene contains an evolutionarily conserved 128-amino acid region referred to as the "Runt domain," which is necessary for binding to...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.24.11723

    authors: Sakakura C,Yamaguchi-Iwai Y,Satake M,Bae SC,Takahashi A,Ogawa E,Hagiwara A,Takahashi T,Murakami A,Makino K

    更新日期:1994-11-22 00:00:00

  • Plasma radioiron kinetics in man: explanation for the effect of plasma iron concentration.

    abstract::The plasma iron turnover was measured in 19 normal subjects. A correlation was found between plasma iron concentration and plasma iron turnover. In addition to the turnover of 55Fe at normal plasma iron concentration (predominantly monoferric transferrin), a second turnover in which the labeled plasma was saturated wi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.75.3.1559

    authors: Skarberg K,Eng M,Huebers H,Marsaglia G,Finch C

    更新日期:1978-03-01 00:00:00

  • Structural basis for high-affinity peptide inhibition of p53 interactions with MDM2 and MDMX.

    abstract::The oncoproteins MDM2 and MDMX negatively regulate the activity and stability of the tumor suppressor protein p53--a cellular process initiated by MDM2 and/or MDMX binding to the N-terminal transactivation domain of p53. MDM2 and MDMX in many tumors confer p53 inactivation and tumor survival, and are important molecul...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0900947106

    authors: Pazgier M,Liu M,Zou G,Yuan W,Li C,Li C,Li J,Monbo J,Zella D,Tarasov SG,Lu W

    更新日期:2009-03-24 00:00:00

  • Endosomal signaling of delta opioid receptors is an endogenous mechanism and therapeutic target for relief from inflammatory pain.

    abstract::Whether G protein-coupled receptors signal from endosomes to control important pathophysiological processes and are therapeutic targets is uncertain. We report that opioids from the inflamed colon activate δ-opioid receptors (DOPr) in endosomes of nociceptors. Biopsy samples of inflamed colonic mucosa from patients an...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2000500117

    authors: Jimenez-Vargas NN,Gong J,Wisdom MJ,Jensen DD,Latorre R,Hegron A,Teng S,DiCello JJ,Rajasekhar P,Veldhuis NA,Carbone SE,Yu Y,Lopez-Lopez C,Jaramillo-Polanco J,Canals M,Reed DE,Lomax AE,Schmidt BL,Leong KW,Vanner SJ,

    更新日期:2020-06-30 00:00:00

  • Optical control of an ion channel gate.

    abstract::The powerful optogenetic pharmacology method allows the optical control of neuronal activity by photoswitchable ligands tethered to channels and receptors. However, this approach is technically demanding, as it requires the design of pharmacologically active ligands. The development of versatile technologies therefore...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1318715110

    authors: Lemoine D,Habermacher C,Martz A,Méry PF,Bouquier N,Diverchy F,Taly A,Rassendren F,Specht A,Grutter T

    更新日期:2013-12-17 00:00:00

  • Microbial phyllosphere populations are more complex than previously realized.

    abstract::Phyllosphere microbial communities were evaluated on leaves of field-grown plant species by culture-dependent and -independent methods. Denaturing gradient gel electrophoresis (DGGE) with 16S rDNA primers generally indicated that microbial community structures were similar on different individuals of the same plant sp...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.051633898

    authors: Yang CH,Crowley DE,Borneman J,Keen NT

    更新日期:2001-03-27 00:00:00

  • Proteomic identification of phosphatidylinositol (3,4,5) triphosphate-binding proteins in Dictyostelium discoideum.

    abstract::Phosphatidylinositol (3,4,5)-triphosphate (PtdInsP(3)) mediates intracellular signaling for directional sensing and pseudopod extension at the leading edge of migrating cells during chemotaxis. How this PtdInsP(3) signal is translated into remodeling of the actin cytoskeleton is poorly understood. Here, using a proteo...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1006153107

    authors: Zhang P,Wang Y,Sesaki H,Iijima M

    更新日期:2010-06-29 00:00:00

  • Theory of muscular contraction extended to groups of actin sites.

    abstract::It was shown in an earlier paper how to connect, in principle, the biochemical states of a cross-bridge with the mechanics of muscular contraction, by the methods of statistical mechanics. The treatment applies to cross-bridges that are able to interact with only one actin site at a time. The present paper shows that ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.70.10.2732

    authors: Hill TL

    更新日期:1973-10-01 00:00:00

  • Serum amyloid A forms stable oligomers that disrupt vesicles at lysosomal pH and contribute to the pathogenesis of reactive amyloidosis.

    abstract::Serum amyloid A (SAA) is an acute-phase plasma protein that functions in innate immunity and lipid homeostasis. SAA is a protein precursor of reactive AA amyloidosis, the major complication of chronic inflammation and one of the most common human systemic amyloid diseases worldwide. Most circulating SAA is protected f...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1707120114

    authors: Jayaraman S,Gantz DL,Haupt C,Gursky O

    更新日期:2017-08-08 00:00:00

  • Efficient replacement of plasma membrane outer leaflet phospholipids and sphingolipids in cells with exogenous lipids.

    abstract::Our understanding of membranes and membrane lipid function has lagged far behind that of nucleic acids and proteins, largely because it is difficult to manipulate cellular membrane lipid composition. To help solve this problem, we show that methyl-α-cyclodextrin (MαCD)-catalyzed lipid exchange can be used to maximally...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1610705113

    authors: Li G,Kim J,Huang Z,St Clair JR,Brown DA,London E

    更新日期:2016-12-06 00:00:00

  • Centromere-encoded RNAs are integral components of the maize kinetochore.

    abstract::RNA is involved in a variety of chromatin modification events, ranging from large-scale structural rearrangements to subtle local affects. Here, we extend the evidence for RNA-chromatin interactions to the centromere core. The data indicate that maize centromeric retrotransposons (CRMs) and satellite repeats (CentC) a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0407154101

    authors: Topp CN,Zhong CX,Dawe RK

    更新日期:2004-11-09 00:00:00

  • Molecular composition of cyanobacterial phycobilisomes.

    abstract::Phycobilisomes isolated from eight different species of cyanobacteria contain in addition to the light-harvesting phycobiliproteins, a small number of colorless polypeptides with molecular weights higher than those of the chromopolypeptide subunits of the phycobiliproteins. In the phycobilisomes of the species examin...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.74.4.1635

    authors: de Marsac NT,Cohen-bazire G

    更新日期:1977-04-01 00:00:00

  • Cloning and sequence analysis of cDNA for the precursor of human growth hormone-releasing factor, somatocrinin.

    abstract::Molecular cloning has established the primary structures of two precursors of the human pancreas growth hormone-releasing factor (hpGRF-44), somatocrinin. Both polypeptides contain the sequence of hpGRF-44 flanked by basic processing sites. Furthermore, the precursors include a putative signal sequence and a carboxyl-...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.80.14.4311

    authors: Gubler U,Monahan JJ,Lomedico PT,Bhatt RS,Collier KJ,Hoffman BJ,Böhlen P,Esch F,Ling N,Zeytin F,Brazeau P,Poonian MS,Gage LP

    更新日期:1983-07-01 00:00:00

  • Characterization of cultured rat oligodendrocytes proliferating in a serum-free, chemically defined medium.

    abstract::A serumless, chemically defined medium has been developed for the culture of oligodendrocytes isolated from primary neonatal rat cerebral cultures. Combined together, insulin, transferrin, and fibroblast growth factor synergistically induced an essentially homogenous population (95-98%) of cells expressing glycerol-3-...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.10.3509

    authors: Saneto RP,de Vellis J

    更新日期:1985-05-01 00:00:00

  • Selective loss of antigen-specific Ir gene function in IA mutant B6.C-H-2bm12 is an antigen presenting cell defect.

    abstract::Immune responses to several soluble antigens were compared between B6.C-H-2bm12 mutant and wild-type B6 mice by using a lymph node T-cell proliferation assay. B6.C-H-2bm12 mice failed to respond to beef insulin whereas other IA gene-controlled responses, such as response to poly(L-Tyr, L-Glu)--poly(DL-Ala, L-Lys) and ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.10.6406

    authors: Lin CC,Rosenthal AS,Passmore HC,Hansen TH

    更新日期:1981-10-01 00:00:00

  • An early role for sonic hedgehog from foregut endoderm in jaw development: ensuring neural crest cell survival.

    abstract::We have investigated the role of Sonic hedgehog (Shh) in the development of facial structures by depriving chicken embryos of the most anterior sources of this morphogen, including the prechordal plate and the anterior ventral endoderm of the foregut, before the onset of neural crest cell (NCC) migration to the first ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0604751103

    authors: Brito JM,Teillet MA,Le Douarin NM

    更新日期:2006-08-01 00:00:00

  • Attraction of specific thalamic input by cerebral grafts depends on the molecular identity of the implant.

    abstract::The cerebral cortex of mammals differentiates into functionally distinct areas that exhibit unique cytoarchitecture, connectivity, and molecular characteristics. Molecular specification of cells fated for limbic cortical areas, based on the expression of the limbic system-associated membrane protein (LAMP), occurs dur...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.9.3706

    authors: Barbe MF,Levitt P

    更新日期:1992-05-01 00:00:00

  • Hitchhiking and epistasis give rise to cohort dynamics in adapting populations.

    abstract::Beneficial mutations are the driving force of adaptive evolution. In asexual populations, the identification of beneficial alleles is confounded by the presence of genetically linked hitchhiker mutations. Parallel evolution experiments enable the recognition of common targets of selection; yet these targets are inhere...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1702314114

    authors: Buskirk SW,Peace RE,Lang GI

    更新日期:2017-08-01 00:00:00

  • Toward a microscopic model of bidirectional synaptic plasticity.

    abstract::We show that a 2-step phospho/dephosphorylation cycle for the alpha-amino-3-hydroxy-5-methyl-4-isoxazole proprionic acid receptor (AMPAR), as used in in vivo learning experiments to assess long-term potentiation (LTP) induction and establishment, exhibits bistability for a wide range of parameters, consistent with val...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0905988106

    authors: Castellani GC,Bazzani A,Cooper LN

    更新日期:2009-08-18 00:00:00

  • Early-life stress has persistent effects on amygdala function and development in mice and humans.

    abstract::Relatively little is known about neurobiological changes attributable to early-life stressors (e.g., orphanage rearing), even though they have been associated with a heightened risk for later psychopathology. Human neuroimaging and animal studies provide complementary insights into the neural basis of problem behavior...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1310163110

    authors: Malter Cohen M,Jing D,Yang RR,Tottenham N,Lee FS,Casey BJ

    更新日期:2013-11-05 00:00:00

  • Correlated dynamics of consecutive residues reveal transient and cooperative unfolding of secondary structure in proteins.

    abstract::Nuclear spin relaxation is a powerful method for studying molecular dynamics at atomic resolution. Recent methods development in biomolecular NMR spectroscopy has enabled detailed investigations of molecular dynamics that are critical for biological function, with prominent examples addressing allostery, enzyme cataly...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0504361102

    authors: Lundström P,Mulder FA,Akke M

    更新日期:2005-11-22 00:00:00

  • Cell line-specific differences in the control of cell cycle progression in the absence of mitosis.

    abstract::This paper reports that there are major differences between mammalian cell lines in the propensity to progress into subsequent cell cycles when mitosis is inhibited with agents that disrupt the assembly of the mitotic spindle apparatus (Colcemid, nocodazole, and taxol). Human HeLa S3 cells, which represent one extreme...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.24.9553

    authors: Kung AL,Sherwood SW,Schimke RT

    更新日期:1990-12-01 00:00:00

  • An archaeal antioxidant: characterization of a Dps-like protein from Sulfolobus solfataricus.

    abstract::Evolution of an oxygenic atmosphere required primordial life to accommodate the toxicity associated with reactive oxygen species. We have characterized an archaeal antioxidant from the hyperthermophilic acidophile Sulfolobus solfataricus. The amino acid sequence of this approximately 22-kDa protein shares little seque...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0501497102

    authors: Wiedenheft B,Mosolf J,Willits D,Yeager M,Dryden KA,Young M,Douglas T

    更新日期:2005-07-26 00:00:00

  • Monoclonal antibodies against trophectoderm-specific markers during mouse blastocyst formation.

    abstract::Two-dimensional gel electrophoresis has allowed the detection of proteins characteristic of inner cell mass and trophectoderm in mouse blastocyst. Certain of the proteins characterizing trophectoderm copurify with intermediate filaments from trophectoderm and a trophoblastoma cell line. A monoclonal antibody prepared ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.77.7.4113

    authors: Brûlet P,Babinet C,Kemler R,Jacob F

    更新日期:1980-07-01 00:00:00

  • Comprehensive copy number and gene expression profiling of the 17q23 amplicon in human breast cancer.

    abstract::The biological significance of DNA amplification in cancer is thought to be due to the selection of increased expression of a single or few important genes. However, systematic surveys of the copy number and expression of all genes within an amplified region of the genome have not been performed. Here we have used a c...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.091582298

    authors: Monni O,Barlund M,Mousses S,Kononen J,Sauter G,Heiskanen M,Paavola P,Avela K,Chen Y,Bittner ML,Kallioniemi A

    更新日期:2001-05-08 00:00:00

  • Calorie restriction prevents the occlusive coronary vascular disease of autoimmune (NZW x BXSB)F1 mice.

    abstract::Male (NZW x BXSB)F1 (W/BF1) mice develop systemic autoimmunity involving autoantibodies, thrombocytopenia, lupus nephritis, and coronary vascular disease (CVD) with myocardial infarction. To determine whether this murine lupus-associated CVD can be prevented by the reduction of dietary calories, male W/BF1 mice were s...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.10.4402

    authors: Mizutani H,Engelman RW,Kinjoh K,Kurata Y,Ikehara S,Matsuzawa Y,Good RA

    更新日期:1994-05-10 00:00:00

  • Messenger RNA for hepatic tryptophan oxygenase: its partial purification, its translation in a heterologous cell-free system, and its control by glucocorticoid hormones.

    abstract::Messenger RNA from rat liver was partially purified by chromatography on cellulose on the basis of its poly(A) content. Microgram amounts of this RNA stimulate protein synthesis manyfold in a heterologous cell-free system, derived from Krebs ascites cells supplemented with reticulocyte initiation factors. The messenge...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.70.4.1218

    authors: Schutz G,Beato M,Feigelson P

    更新日期:1973-04-01 00:00:00

  • Immuno-Navigator, a batch-corrected coexpression database, reveals cell type-specific gene networks in the immune system.

    abstract::High-throughput gene expression data are one of the primary resources for exploring complex intracellular dynamics in modern biology. The integration of large amounts of public data may allow us to examine general dynamical relationships between regulators and target genes. However, obstacles for such analyses are stu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1604351113

    authors: Vandenbon A,Dinh VH,Mikami N,Kitagawa Y,Teraguchi S,Ohkura N,Sakaguchi S

    更新日期:2016-04-26 00:00:00

  • Single amino acid substitution in Plasmodium yoelii erythrocyte ligand determines its localization and controls parasite virulence.

    abstract::The major virulence determinant of the rodent malaria parasite, Plasmodium yoelii, has remained unresolved since the discovery of the lethal line in the 1970s. Because virulence in this parasite correlates with the ability to invade different types of erythrocytes, we evaluated the potential role of the parasite eryth...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0811313106

    authors: Otsuki H,Kaneko O,Thongkukiatkul A,Tachibana M,Iriko H,Takeo S,Tsuboi T,Torii M

    更新日期:2009-04-28 00:00:00

  • New Integral Representations for Solutions of Cauchy's Problem for Abstract Parabolic Equations.

    abstract::A study has been made of Cauchy's problem for a class of abstract parabolic differential equations. Our study is based upon a transformation that maps solutions of second-order abstract Cauchy problems into solutions of first-order. This is a preliminary report on some of the results obtained. These results include (1...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.68.9.2025

    authors: Donaldson JA

    更新日期:1971-09-01 00:00:00