Abstract:
:Mismatch repair (MMR) is a major DNA repair pathway in cells from all branches of life that removes replication errors in a strand-specific manner, such that mismatched nucleotides are preferentially removed from the newly replicated strand of DNA. Here we demonstrate a role for MMR in helping create new phenotypes in nondividing cells. We show that mispairs in yeast that escape MMR during replication can later be subject to MMR activity in a replication strand-independent manner in nondividing cells, resulting in either fully wild-type or mutant DNA sequence. In one case, this activity is responsible for what appears to be adaptive mutation. This replication strand-independent MMR activity could contribute to the formation of tumors arising in nondividing cells and could also contribute to mutagenesis observed during somatic hypermutation of Ig genes.
journal_name
Proc Natl Acad Sci U S Aauthors
Rodriguez GP,Romanova NV,Bao G,Rouf NC,Kow YW,Crouse GFdoi
10.1073/pnas.1115361109subject
Has Abstractpub_date
2012-04-17 00:00:00pages
6153-8issue
16eissn
0027-8424issn
1091-6490pii
1115361109journal_volume
109pub_type
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