Gi- and Gs-coupled GPCRs show different modes of G-protein binding.

abstract：:The unique ability of bovine papillomavirus (BPV-1) DNA to replicate as a stable, multicopy plasmid in transformed mouse cells has led to its utilization as a eukaryotic cloning vector. One limitation of the system has been the marked reduction in transformation efficiency when BPV-1 DNA or the subgenomic transforming...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sarver N,Byrne JC,Howley PM

更新日期：1982-12-01 00:00:00

abstract：:A solid-phase immunoadsorbent capable of binding mouse interferon has been prepared. Starting from crude tissue-culture material, interferon could be purified 1990 times in a single step of affinity chromatography. Overall recovery ranged from 55 to 103% with tissue culture and mouse-brain interferon; however, only 5%...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sipe JD,de Maeyer-Guignard J,Fauconnier B,de Maeyer E

更新日期：1973-04-01 00:00:00

abstract：:The GATA factor Serpent (Srp) is required for hemocyte precursor formation during Drosophila hematopoiesis. These blood cell progenitors give rise to two distinct lineages within the developing embryo. Lozenge, a Runx protein homologue, and Glial cells missing-1 and -2 are essential for crystal cell and plasmatocyte p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fossett N,Hyman K,Gajewski K,Orkin SH,Schulz RA

更新日期：2003-09-30 00:00:00

abstract：:For brain functions such as working memory and motor planning, neuronal circuits are able to sustain persistent activity after transient inputs. Theoretical studies have suggested that persistent activity can exist in recurrently connected networks as active reverberation. However, the actual cellular processes underl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lau PM,Bi GQ

更新日期：2005-07-19 00:00:00

abstract：:The use of computers to simulate the functions of complex biological macromolecules is essential to achieve a microscopic description of biological processes and to model and interpret experimental data. Here we apply theoretical computational approaches to investigate the fidelity of T7 DNA polymerase, divided into d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Florián J,Goodman MF,Warshel A

更新日期：2005-05-10 00:00:00

abstract：:Identifying targets is critical for understanding the biological effects of microRNA (miRNA) expression. The challenge lies in characterizing the cohort of targets for a specific miRNA, especially when targets are being actively down-regulated in miRNA- RNA-induced silencing complex (RISC)-messengerRNA (mRNA) complexe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cambronne XA,Shen R,Auer PL,Goodman RH

更新日期：2012-12-11 00:00:00

abstract：:Here, we identified and characterized a Ly49 family member, designated as Ly49Q. The Ly49q gene encodes a 273-aa protein with an immunoreceptor tyrosine-based inhibitory motif (ITIM) at the N terminus of its cytoplasmic domain. We show that the ITIM of Ly49Q can recruit SHP-2 and SHP-1 in a tyrosine phosphorylation-de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toyama-Sorimachi N,Tsujimura Y,Maruya M,Onoda A,Kubota T,Koyasu S,Inaba K,Karasuyama H

更新日期：2004-01-27 00:00:00

abstract：:The origins of the five groups of living seed plants, including the single relictual species Ginkgo biloba, are poorly understood, in large part because of very imperfect knowledge of extinct seed plant diversity. Here we describe well-preserved material from the Early Cretaceous of Mongolia of the previously enigmati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Herrera F,Shi G,Ichinnorov N,Takahashi M,Bugdaeva EV,Herendeen PS,Crane PR

更新日期：2017-03-21 00:00:00

abstract：:We have purified a protein from Necturus maculosus gallbladder cells that forms chloride channels in an artificial membrane. The same protein apparently can form channels that are highly selective for chloride but can have conductances varying from 9 to about 150 pS. The high-conductance channels are blocked by the mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Finn AL,Dillard M,Gaido M

更新日期：1993-06-15 00:00:00

abstract：:Monoclonal antibodies were used to isolate nonneutralizable antigenic variants in the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus. It had been found that a large percentage of virus retains infectivity despite binding neutralizing antibody. This high persistent fraction of nonneutralized virus ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iorio RM,Bratt MA

更新日期：1985-10-01 00:00:00

abstract：:Cereal agriculture originated with the domestication of barley and early forms of wheat in the Fertile Crescent. There has long been speculation that barley was domesticated more than once. We use differences in haplotype frequency among geographic regions at multiple loci to infer at least two domestications of barle...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morrell PL,Clegg MT

更新日期：2007-02-27 00:00:00

abstract：:Metallo-β-lactamases (MBLs) hydrolyze almost all β-lactam antibiotics and are unaffected by clinically available β-lactamase inhibitors (βLIs). Active-site architecture divides MBLs into three classes (B1, B2, and B3), complicating development of βLIs effective against all enzymes. Bisthiazolidines (BTZs) are carboxyl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hinchliffe P,González MM,Mojica MF,González JM,Castillo V,Saiz C,Kosmopoulou M,Tooke CL,Llarrull LI,Mahler G,Bonomo RA,Vila AJ,Spencer J

更新日期：2016-06-28 00:00:00

abstract：:Heterogeneous nucleation is vital to a wide range of areas as diverse as ice nucleation on atmospheric aerosols and the fabrication of high-performance thin films. There is excellent evidence that surface topography is a key factor in directing crystallization in real systems; however, the mechanisms by which nanoscal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Campbell JM,Meldrum FC,Christenson HK

更新日期：2017-01-31 00:00:00

abstract：:The ability of antibodies to extravasate out of blood vessels is critical for therapeutic activity, because molecular targets for most diseases are located outside of the endothelial lining. By performing detailed biodistribution studies with a novel IL9-armed cancer-specific antibody, we identified a clear correlatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Venetz D,Hess C,Lin CW,Aebi M,Neri D

更新日期：2015-02-17 00:00:00

abstract：:Calcitonin gene-related peptide (CGRP) is a neuropeptide that coexists with acetylcholine in spinal cord motoneurons. The effects of CGRP on the functional properties of the nicotinic acetylcholine receptor (AcChoR) were examined by electrophysiological methods. Using the whole-cell patch-clamp technique and a mouse c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mulle C,Benoit P,Pinset C,Roa M,Changeux JP

更新日期：1988-08-01 00:00:00

abstract：:Various concentrations of L-dopa (levodopa, L-3,4-dihydroxyphenylalanine) were incorporated in the diets of young male Swiss albino mice, and the highest concentrations to which these mice became adapted within 3 weeks were determined. One such concentration administered indefinitely eventually induced a youthful appe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cotzias GC,Miller ST,Nicholson AR Jr,Maston WH,Tang LC

更新日期：1974-06-01 00:00:00

abstract：:Vesicles were constituted with glycophorin, the Sendai virus receptor of human erythrocytes, and loaded with calcein, a polar derivative of fluorescein, at self-quenching concentrations. On exposure to Sendai virus and mild hypo-osmotic stress, vesicles of the appropriate composition released a significant portion of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kundrot CE,Spangler EA,Kendall DA,MacDonald RC,MacDonald RI

更新日期：1983-03-01 00:00:00

abstract：:The study of development has relied primarily on the isolation of mutations in genes with specific functions in development and on the comparison of their expression patterns in normal and mutant phenotypes. Comparative evolutionary analyses can complement these approaches. Phylogenetic analyses of Sonic hedgehog (Shh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zardoya R,Abouheif E,Meyer A

更新日期：1996-11-12 00:00:00

abstract：:Six Rhesus monkeys between 24 and 29 years of age received unilateral transections of the fornix. Three monkeys then received intraventricular transplants of polymer-encapsulated baby hamster kidney (BHK) fibroblasts that had been genetically modified to secrete human nerve growth factor (hNGF). The remaining three mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kordower JH,Winn SR,Liu YT,Mufson EJ,Sladek JR Jr,Hammang JP,Baetge EE,Emerich DF

更新日期：1994-11-08 00:00:00

abstract：:Supercritical fluids are a unique class of nonaqueous media in which biocatalytic reactions can occur. The physical properties of supercritical fluids, which include gas-like diffusivities and liquid-like densities, can be predictably controlled with changing pressure. This paper describes how adjustment of pressure, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kamat SV,Iwaskewycz B,Beckman EJ,Russell AJ

更新日期：1993-04-01 00:00:00

abstract：:Collectivistic cultures have been characterized as having harmonious, cooperative ingroup relationships. However, we find evidence that people in collectivistic cultures are more vigilant toward ingroup members, mindful of their possible unethical intentions. Study 1 found that Chinese participants were more vigilant ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu SS,Morris MW,Talhelm T,Yang Q

更新日期：2019-07-16 00:00:00

abstract：:An intermolecular intercalation of base pairs was found at the CA step in the I222 crystal structure of the RNA.DNA hybrid, r(CAAAGAAAAG).d(CTTTTCTTTG), which contains two-thirds of the polypurine tract sequence of HIV-1 with a substitution of cytosine for the initial adenine. This sequence crystallized in both P21212...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han GW,Kopka ML,Langs D,Sawaya MR,Dickerson RE

更新日期：2003-08-05 00:00:00

abstract：:Compartmentalization of proteins into subcellular organelles in eukaryotic cells is a fundamental mechanism of regulating complex cellular functions. Many proteins of Plasmodium falciparum merozoites involved in invasion are compartmentalized into apical organelles. We have identified a new merozoite organelle that co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singh S,Plassmeyer M,Gaur D,Miller LH

更新日期：2007-12-11 00:00:00

abstract：:Transfection of cells with the H-2Kk gene lacking the transmembrane and cytoplasmic segments resulted in secretion of the H-2Kk protein, as determined by immunoprecipitation with monoclonal anti-H-2Kk antibodies. Transgenic (H-2b X H-2d)F1 mice were established carrying integrated copies of the modified H-2Kk gene. Ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arnold B,Dill O,Küblbeck G,Jatsch L,Simon MM,Tucker J,Hämmerling GJ

更新日期：1988-04-01 00:00:00

abstract：:Previous studies have identified human breast tumor particles possessing many of the features characteristic of RNA tumor viruses. In addition to the expected size (600 S) and density (1.16 g/ml) these include possession of an outer membrane and an inner one surrounding a "core" containing a DNA polymerase and a large...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohno T,Sweet RW,Dejak D,Spiegelman S

更新日期：1977-02-01 00:00:00

abstract：:Aerobic glycolysis (AG), that is, the nonoxidative metabolism of glucose, contributes significantly to anabolic pathways, rapid energy generation, task-induced activity, and neuroprotection; yet high AG is also associated with pathological hallmarks such as amyloid-β deposition. An important yet unresolved question is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen Y,Lin Q,Liao X,Zhou C,He Y

更新日期：2021-01-05 00:00:00

abstract：:Vanadyl sulfate and sodium orthovanadate in the concentration range between 5 and 50 microM are shown to be mitogenic for quiescent cultures of Swiss mouse 3T3 and 3T6 cells. The compounds caused a striking shift in the dose-response for the effect of serum on [3H]thymidine incorporation and DNA synthesis. In the abse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith JB

更新日期：1983-10-01 00:00:00

abstract：:The plant hormones abscisic acid (ABA) and gibberellic acid (GA) are important regulators of the dormancy and germination of seeds. In cereals, GA enhances the synthesis and secretion of enzymes (principally alpha-amylases) in the aleurone cells of the endosperm, which then mobilize the storage reserves that fuel germ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ritchie S,Gilroy S

更新日期：1998-03-03 00:00:00

abstract：:The postdevelopmental basis of cellular identity and the unique cellular output of a particular neuron type are of particular interest in the nervous system because a detailed understanding of circuits responsible for complex processes in the brain is impeded by the often ambiguous classification of neurons in these c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz DJ,Goaillard JM,Marder EE

更新日期：2007-08-07 00:00:00

abstract：:Cerebellar development occurs mainly postnatally and implies cell proliferation and migration. Hepatocyte growth factor (HGF) and Met are involved in mediating these responses in other tissues and are coexpressed in the cerebellum. Here we show that Met is localized in granule cell precursors and that cultures of thes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ieraci A,Forni PE,Ponzetto C

更新日期：2002-11-12 00:00:00