当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Purification and characterization of the DNA polymerase of human breast cancer particles.

Purification and characterization of the DNA polymerase of human breast cancer particles.

Abstract：

:Previous studies have identified human breast tumor particles possessing many of the features characteristic of RNA tumor viruses. In addition to the expected size (600 S) and density (1.16 g/ml) these include possession of an outer membrane and an inner one surrounding a "core" containing a DNA polymerase and a large-molecular-weight (70S) RNA possessing detectable homology to the RNAs of the mouse mammary tumor virus (MMTV) and of the Mason-Pfizer monkey virus (MPMV). We report here the purification and characterization of the DNA polymerase from the human breast cancer particles. Its key properties are very similar to those ofthe RNA-dependent DNA nucleotidyltransferase (reverse transcriptase) found in MMTV and MPMV. Thus like these viral enzymes, the purified human breast cancer DNA polymerase exhibits the following three features that together distinguish the known viral reverse transcriptases from normal cellular DNA polymerases: (i) a strong preference for oligo(dT)-poly(rA) over oligo(dT)-poly(dA) as a template for the synthesis of poly(dT); (ii) the acceptance of the highly specific oligo(dG)-poly(rCm) as a template for the formation of poly(dG); (iii) the ability to use a viral RNA (AMV) as a template to fashion a faithful DNA complementary copy; and (iv) its preference for Mg++ over Mn++. In summary, the data described here on the enzyme of the human breast cancer particles add further evidence of similarities to the viral agents associated with the corresponding malignancies in the mouse and monkey models. To date, an enzyme with these properties has not been detected in normal breast tissues or in benign tumors of the breast.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Ohno T,Sweet RW,Dejak D,Spiegelman S

doi

10.1073/pnas.74.2.764

subject

Has Abstract

pub_date

1977-02-01 00:00:00

pages

764-8

issue

2

eissn

0027-8424

issn

1091-6490

journal_volume

74

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Transcriptional repression of euchromatic genes by Drosophila heterochromatin protein 1 and histone modifiers.

    abstract：:In Drosophila, heterochromatin protein 1 (HP1) suppresses the expression of euchromatic genes that are artificially translocated adjacent to heterochromatin by expanding heterochromatin structure into neighboring euchromatin. The purpose of this study was to determine whether HP1 functions as a transcriptional repress...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.211303598

    authors： Hwang KK,Eissenberg JC,Worman HJ

    更新日期：2001-09-25 00:00:00

  • Effect of glycosylation on protein folding: a close look at thermodynamic stabilization.

    abstract：:Glycosylation is one of the most common posttranslational modifications to occur in protein biosynthesis, yet its effect on the thermodynamics and kinetics of proteins is poorly understood. A minimalist model based on the native protein topology, in which each amino acid and sugar ring was represented by a single bead...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0801340105

    authors： Shental-Bechor D,Levy Y

    更新日期：2008-06-17 00:00:00

  • Phosphate metabolism in intact human erythrocytes: determination by phosphorus-31 nuclear magnetic resonance spectroscopy.

    abstract：:Whole human blood was examined by (31)P nuclear magnetic resonance spectroscopy. Individual phosphates (alpha,beta,gamma) of ATP were identifiable, and two microenvironments appeared to be present for this molecule. When sequential recordings of freshly collected blood were made, 2,3-diphosphoglycerate was observed to...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.6.2487

    authors： Henderson TO,Costello AJ,Omachi A

    更新日期：1974-06-01 00:00:00

  • Design of a "green" one-step catalytic production of epsilon-caprolactam (precursor of nylon-6).

    abstract：:The ever-increasing industrial demand for nylon-6 (polycaprolactam) necessitates the development of environmentally benign methods of producing its precursor, epsilon-caprolactam, from cyclohexanone. It is currently manufactured in two popular double-step processes, each of which uses highly aggressive reagents, and e...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0506907102

    authors： Thomas JM,Raja R

    更新日期：2005-09-27 00:00:00

  • DNase I-hypersensitive sites in the chromatin of immunoglobulin kappa light chain genes.

    abstract：:We have used DNase I as a probe to examine the chromatin structure of mouse immunoglobulin kappa light chain genes in rearranged and unrearranged chromosomes--i.e., in nuclei from myeloma cells and from brain and liver cells. Tissue-specific DNase I-hypersensitive sites are observed 0.7 and 1.7 kilobases upstream from...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.9.2427

    authors： Chung SY,Folsom V,Wooley J

    更新日期：1983-05-01 00:00:00

  • Loss of microRNA cluster miR-29a/b-1 in sporadic Alzheimer's disease correlates with increased BACE1/beta-secretase expression.

    abstract：:Although the role of APP and PSEN genes in genetic Alzheimer's disease (AD) cases is well established, fairly little is known about the molecular mechanisms affecting Abeta generation in sporadic AD. Deficiency in Abeta clearance is certainly a possibility, but increased expression of proteins like APP or BACE1/beta-s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0710263105

    authors： Hébert SS,Horré K,Nicolaï L,Papadopoulou AS,Mandemakers W,Silahtaroglu AN,Kauppinen S,Delacourte A,De Strooper B

    更新日期：2008-04-29 00:00:00

  • Nitrogen regulator GlnR controls uptake and utilization of non-phosphotransferase-system carbon sources in actinomycetes.

    abstract：:The regulatory mechanisms underlying the uptake and utilization of multiple types of carbohydrates in actinomycetes remain poorly understood. In this study, we show that GlnR (central regulator of nitrogen metabolism) serves as a universal regulator of nitrogen metabolism and plays an important, previously unknown rol...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1508465112

    authors： Liao CH,Yao L,Xu Y,Liu WB,Zhou Y,Ye BC

    更新日期：2015-12-22 00:00:00

  • UV-endonuclease from calf thymus with specificity toward pyrimidine dimers in DNA.

    abstract：:We describe the partial purification of an endonuclease from calf thymus that nicks phage PM2 DNA irradiated with UV doses producing only a few pyrimidine dimers per molecule. It has much less activity on DNA that has been subjected to enzymatic photoreactivation after UV irradiation. The calf thymus endonuclease is d...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.8.3746

    authors： Waldstein EA,Peller S,Setlow RB

    更新日期：1979-08-01 00:00:00

  • Apoptosis of nur77/N10-transgenic thymocytes involves the Fas/Fas ligand pathway.

    abstract：:The orphan nuclear receptor Nur77/N10 has recently been demonstrated to be involved in apoptosis of T cell hybridomas. We report here that chronic expression of Nur77/N10 in thymocytes of transgenic mice results in a dramatic reduction of CD4+CD8+ double-positive as well as CD4+CD8- and CD4-CD8+ single-positive cell p...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.11.5533

    authors： Weih F,Ryseck RP,Chen L,Bravo R

    更新日期：1996-05-28 00:00:00

  • Isolation of supercoiled colicinogenic factor E 1 DNA sensitive to ribonuclease and alkali.

    abstract：:The synthesis of the covalently-closed, circular DNA form of colicinogenic factor E(1) (ColE(1)) continues in Escherichia coli cells after the addition of chloramphenicol. A large portion of the purified supercoiled ColE(1) DNA molecules made in the presence of chloramphenicol are converted to the open circular DNA fo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.69.9.2518

    authors： Blair DG,Sherratt DJ,Clewell DB,Helinski DR

    更新日期：1972-09-01 00:00:00

  • Attenuation of thermal nociception and hyperalgesia by VR1 blockers.

    abstract：:Vanilloid receptor subunit 1 (VR1) appears to play a critical role in the transduction of noxious chemical and thermal stimuli by sensory nerve endings in peripheral tissues. Thus, VR1 antagonists are useful compounds to unravel the contribution of this receptor to pain perception, as well as to induce analgesia. We h...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.022285899

    authors： García-Martinez C,Humet M,Planells-Cases R,Gomis A,Caprini M,Viana F,De La Pena E,Sanchez-Baeza F,Carbonell T,De Felipe C,Pérez-Paya E,Belmonte C,Messeguer A,Ferrer-Montiel A

    更新日期：2002-02-19 00:00:00

  • Altered pharmacology and distinct coactivator usage for estrogen receptor-dependent transcription through activating protein-1.

    abstract：:Estrogen signaling occurs through at least two distinct molecular pathways: (i) direct binding of liganded estrogen receptors (ERs) to estrogen-responsive DNA elements (EREs) (the "ER/ERE pathway") and (ii) indirect recruitment of liganded ERs to activating protein-1 (AP-1)-responsive DNA elements via heterodimers of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0407113102

    authors： Cheung E,Acevedo ML,Cole PA,Kraus WL

    更新日期：2005-01-18 00:00:00

  • BK potassium channel modulation by leucine-rich repeat-containing proteins.

    abstract：:Molecular diversity of ion channel structure and function underlies variability in electrical signaling in nerve, muscle, and nonexcitable cells. Regulation by variable auxiliary subunits is a major mechanism to generate tissue- or cell-specific diversity of ion channel function. Mammalian large-conductance, voltage- ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1205435109

    authors： Yan J,Aldrich RW

    更新日期：2012-05-15 00:00:00

  • gamma-Aminobutyric acid exerts a local inhibitory action on the axon terminal of bipolar cells: evidence for negative feedback from amacrine cells.

    abstract：:It is well-established morphologically that bipolar cells, the second-order neurons in the vertebrate retina, make reciprocal synapses with amacrine cells in the inner plexiform layer. However, neither the property nor the physiological function of the feedback synapse is understood. Autoradiographic and immunohistoch...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.10.3501

    authors： Tachibana M,Kaneko A

    更新日期：1987-05-01 00:00:00

  • Ataxia telangiectasia-mutated protein and DNA-dependent protein kinase have complementary V(D)J recombination functions.

    abstract：:Antigen receptor variable region exons are assembled during lymphocyte development from variable (V), diversity (D), and joining (J) gene segments. Each germ-line gene segment is flanked by recombination signal sequences (RSs). Recombination-activating gene endonuclease initiates V(D)J recombination by cleaving a pair...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1019293108

    authors： Zha S,Jiang W,Fujiwara Y,Patel H,Goff PH,Brush JW,Dubois RL,Alt FW

    更新日期：2011-02-01 00:00:00

  • CD1d-Restricted pathways in hepatocytes control local natural killer T cell homeostasis and hepatic inflammation.

    abstract：:Invariant natural killer T (iNKT) cells recognize lipid antigens presented by CD1d and play a central role in regulating immunity and inflammation in peripheral tissues. However, the mechanisms which govern iNKT cell homeostasis after thymic emigration are incompletely understood. Here we demonstrate that microsomal t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1701428114

    authors： Zeissig S,Peuker K,Iyer S,Gensollen T,Dougan SK,Olszak T,Kaser A,Blumberg RS

    更新日期：2017-09-26 00:00:00

  • Neisseria gonorrhoeae-derived heptose elicits an innate immune response and drives HIV-1 expression.

    abstract：:Clinical and epidemiological synergy exists between the globally important sexually transmitted infections, gonorrhea and HIV. Neisseria gonorrhoeae, which causes gonorrhea, is particularly adept at driving HIV-1 expression, but the molecular determinants of this relationship remain undefined. N. gonorrhoeae liberates...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1303738110

    authors： Malott RJ,Keller BO,Gaudet RG,McCaw SE,Lai CC,Dobson-Belaire WN,Hobbs JL,St Michael F,Cox AD,Moraes TF,Gray-Owen SD

    更新日期：2013-06-18 00:00:00

  • Identification of a heritable deficiency of the folate-dependent enzyme 10-formyltetrahydrofolate dehydrogenase in mice.

    abstract：:During the analysis of liver protein expression in the offspring of male mice irradiated with fission-spectrum neutrons, one offspring displayed a heritable 50% decrease in the abundance of two proteins. Homozygous mice lacking detectable quantities of these proteins were obtained through breeding. Characterization of...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.24.11338

    authors： Champion KM,Cook RJ,Tollaksen SL,Giometti CS

    更新日期：1994-11-22 00:00:00

  • DNA methylation in the gene body influences MeCP2-mediated gene repression.

    abstract：:Rett syndrome is a severe neurodevelopmental disorder caused by mutations in the methyl-CpG binding protein gene (MECP2). MeCP2 is a methyl-cytosine binding protein that is proposed to function as a transcriptional repressor. However, multiple gene expression studies comparing wild-type and MeCP2-deficient neurons hav...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1618737114

    authors： Kinde B,Wu DY,Greenberg ME,Gabel HW

    更新日期：2016-12-27 00:00:00

  • Chronic inflammation that facilitates tumor progression creates local immune suppression by inducing indoleamine 2,3 dioxygenase.

    abstract：:Topical application of phorbol myristate acetate (PMA) elicits intense local inflammation that facilitates outgrowth of premalignant lesions in skin after carcinogen exposure. The inflammatory response to PMA treatment activates immune stimulatory mechanisms. However, we show here that PMA exposure also induces plasma...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0806173105

    authors： Muller AJ,Sharma MD,Chandler PR,Duhadaway JB,Everhart ME,Johnson BA 3rd,Kahler DJ,Pihkala J,Soler AP,Munn DH,Prendergast GC,Mellor AL

    更新日期：2008-11-04 00:00:00

  • Somatostatin: an endogenous peptide in the toad urinary bladder inhibits vasopressin-stimulated water flow.

    abstract：:Somatostatin (somatotropin release-inhibiting factor; SRIF) is a tetradecapeptide present in brain, pancreas, gastrointestinal tract, and thyroid that inhibits the secretion or action of several hormones in these tissues. We observed that the toad urinary bladder contains concentrations of endogenous somatostatin (8.0...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.8.4984

    authors： Forrest JN Jr,Reichlin S,Goodman DB

    更新日期：1980-08-01 00:00:00

  • Congruences between modular forms: raising the level and dropping Euler factors.

    abstract：:We discuss the relationship among certain generalizations of results of Hida, Ribet, and Wiles on congruences between modular forms. Hida's result accounts for congruences in terms of the value of an L-function, and Ribet's result is related to the behavior of the period that appears there. Wiles' theory leads to a cl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.21.11143

    authors： Diamond F

    更新日期：1997-10-14 00:00:00

  • Assembly and subunit stoichiometry of the functional helicase-primase (primosome) complex of bacteriophage T4.

    abstract：:Physical biochemical techniques are used to establish the structure, subunit stoichiometry, and assembly pathway of the primosome complex of the bacteriophage T4 DNA replication system. Analytical ultracentrifugation and fluorescence anisotropy methods show that the functional T4 primosome consists of six gp41 helicas...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1210040109

    authors： Jose D,Weitzel SE,Jing D,von Hippel PH

    更新日期：2012-08-21 00:00:00

  • Left-handed Z-DNA in bands of acid-fixed polytene chromosomes.

    abstract：:Antibodies to DNA in the left-handed (Z) conformation bind to acid-fixed polytene chromosomes of both Chironomus thummi and Drosophila melanogaster, as shown by direct and indirect immunofluorescence. Comparison of the phase-contrast, immunofluorescence, and DNA staining patterns shows a predominant localization of th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.14.4344

    authors： Arndt-Jovin DJ,Robert-Nicoud M,Zarling DA,Greider C,Weimer E,Jovin TM

    更新日期：1983-07-01 00:00:00

  • Reach and speed of judgment propagation in the laboratory.

    abstract：:In recent years, a large body of research has demonstrated that judgments and behaviors can propagate from person to person. Phenomena as diverse as political mobilization, health practices, altruism, and emotional states exhibit similar dynamics of social contagion. The precise mechanisms of judgment propagation are ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1611998114

    authors： Moussaïd M,Herzog SM,Kämmer JE,Hertwig R

    更新日期：2017-04-18 00:00:00

  • Low-cost high-efficiency system for solar-driven conversion of CO2 to hydrocarbons.

    abstract：:Conversion of carbon dioxide into hydrocarbons using solar energy is an attractive strategy for storing such a renewable source of energy into the form of chemical energy (a fuel). This can be achieved in a system coupling a photovoltaic (PV) cell to an electrochemical cell (EC) for CO2 reduction. To be beneficial and...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1815412116

    authors： Huan TN,Dalla Corte DA,Lamaison S,Karapinar D,Lutz L,Menguy N,Foldyna M,Turren-Cruz SH,Hagfeldt A,Bella F,Fontecave M,Mougel V

    更新日期：2019-05-14 00:00:00

  • Systemic administration of beta-endorphin: potent hypotensive effect involving a serotonergic pathway.

    abstract：:In normal adult rats anesthetized with urethane, intravenous injections of beta-endorphin (30--150 micrograms kg-1) induced a transient fall of blood pressure followed by a small hypertension and a prolonged hypotension. Prior administration of naloxone completely blocked these effects, whereas naloxone, given 1 hr af...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.12.6240

    authors： Lemaire I,Tseng R,Lemaire S

    更新日期：1978-12-01 00:00:00

  • Maternal and zygotic Zfp57 modulate NOTCH signaling in cardiac development.

    abstract：:Zfp57 is a maternal-zygotic effect gene that maintains genomic imprinting. Here we report that Zfp57 mutants exhibited a variety of cardiac defects including atrial septal defect (ASD), ventricular septal defect (VSD), thin myocardium, and reduced trabeculation. Zfp57 maternal-zygotic mutant embryos displayed more sev...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1415541112

    authors： Shamis Y,Cullen DE,Liu L,Yang G,Ng SF,Xiao L,Bell FT,Ray C,Takikawa S,Moskowitz IP,Cai CL,Yang X,Li X

    更新日期：2015-04-21 00:00:00

  • PTEN signaling through RAF1 proto-oncogene serine/threonine kinase (RAF1)/ERK in the epididymis is essential for male fertility.

    abstract：:Without a fully developed initial segment, the most proximal region of the epididymis, male infertility results. Therefore, it is important to understand the development and regulation of this crucial region. In addition to distinctively high activity levels of the components of the ERK pathway, which are essential fo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1413186112

    authors： Xu B,Washington AM,Hinton BT

    更新日期：2014-12-30 00:00:00

  • DNA conformation on surfaces measured by fluorescence self-interference.

    abstract：:The conformation of DNA molecules tethered to the surface of a microarray may significantly affect the efficiency of hybridization. Although a number of methods have been applied to determine the structure of the DNA layer, they are not very sensitive to variations in the shape of DNA molecules. Here we describe the a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0511214103

    authors： Moiseev L,Unlü MS,Swan AK,Goldberg BB,Cantor CR

    更新日期：2006-02-21 00:00:00