The aged monkey basal forebrain: rescue and sprouting of axotomized basal forebrain neurons after grafts of encapsulated cells secreting human nerve growth factor.

abstract：:Freshwater planarian flatworms are capable of regenerating complete organisms from tiny fragments of their bodies; the basis for this regenerative prowess is an experimentally accessible stem cell population that is present in the adult planarian. The study of these organisms, classic experimental models for investiga...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Newmark PA,Reddien PW,Cebrià F,Sánchez Alvarado A

更新日期：2003-09-30 00:00:00

abstract：:Mutations in the presenilin 1 (PS1) and presenilin 2 (PS2) genes increase the production of the highly amyloidogenic 42-residue form of amyloid beta-protein (Abeta42) in a variety of cell lines and transgenic mice. To elucidate the molecular mechanism of this effect, wild-type (wt) or mutant PS1 and PS2 genes were sta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xia W,Zhang J,Perez R,Koo EH,Selkoe DJ

更新日期：1997-07-22 00:00:00

abstract：:Nuclear import of classical nuclear localization sequence-bearing proteins is mediated by karyopherin alpha/beta1 heterodimers. A second nuclear import pathway, mediated by karyopherin beta2 (transportin), recently was described for mRNA-binding proteins. Here we report the cloning and characterization of human karyop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yaseen NR,Blobel G

更新日期：1997-04-29 00:00:00

abstract：:We described previously a mutation in maize, hcf106, caused by the insertion of a Mu1 transposon. When the Mu transposon system is in an active phase, hcf106 conditions a nonphotosynthetic, pale green phenotype. However, when the Mu system is inactive (a state correlated with hypermethylation of Mu elements), the plan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barkan A,Martienssen RA

更新日期：1991-04-15 00:00:00

abstract：:Glucagon and insulin are first detectable at the onset of rat pancreas organogenesis. Initially, the specific activity of glucagon is approximately 100-fold higher than that of insulin. At this early stage, endocrine storage granules, similar to alpha granules, are identifiable in electron micrographs. The granule cha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rall LB,Pictet RL,Williams RH,Rutter WJ

更新日期：1973-12-01 00:00:00

abstract：:A sensitive assay using biotinylated ubiquitin revealed extensive ubiquitination of the large subunit of RNA polymerase II during incubations of transcription reactions in vitro. Phosphorylation of the repetitive carboxyl-terminal domain of the large subunit was a signal for ubiquitination. Specific inhibitors of cycl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitsui A,Sharp PA

更新日期：1999-05-25 00:00:00

abstract：:Gene amplification may be visualized within a chromosome as a homogeneously stained region (HSR) and HSRs have rarely been reported in human tumor cells with identification of the amplified gene. A parental line and seven clones derived from KB cells resistant to methotrexate (MTX) contain dihydrofolate reductase (DHF...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolman SR,Craven ML,Grill SP,Domin BA,Cheng YC

更新日期：1983-02-01 00:00:00

abstract：:Analysis of the nucleotide and deduced amino acid sequences of the biologically active mouse mdr1 cDNA clone indicates that the protein is formed by two highly homologous halves, each containing six putative transmembrane domains and a nucleotide-binding site. The duplicated unit shows high sequence homology to the pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raymond M,Gros P

更新日期：1989-09-01 00:00:00

abstract：:We report light-induced electron paramagnetic resonance triplet spectra from samples of chloroplasts or digitonin photosystem I particles that depend upon the dark redox state of the bound acceptors of photosystem I. If the reaction centers are prepared in the redox state P-700 A X- FdB-FdA-, then upon illumination at...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frank HA,McLean MB,Sauer K

更新日期：1979-10-01 00:00:00

abstract：:We have developed a defective herpes simplex virus (HSV) vector system that permits the introduction of virtually any gene into mammalian central nervous system neurons. The prototype vector, pHSVlac, contains a transcription unit that places the Escherichia coli lacZ gene under the control of the HSV-1 immediate earl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geller AI,Freese A

更新日期：1990-02-01 00:00:00

abstract：:Transgenic sorghum plants have been obtained after microprojectile bombardment of immature zygotic embryos of a drought-resistant sorghum cultivar, P898012. DNA delivery parameters were optimized based on transient expression of R and C1 maize anthocyanin regulatory elements in scutellar cells. The protocol for obtain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Casas AM,Kononowicz AK,Zehr UB,Tomes DT,Axtell JD,Butler LG,Bressan RA,Hasegawa PM

更新日期：1993-12-01 00:00:00

abstract：:Three lines of evidence for abrupt tropical climate change, both past and present, are presented. First, annually and decadally averaged delta(18)O and net mass-balance histories for the last 400 and 2,000 yr, respectively, demonstrate that the current warming at high elevations in the mid- to low latitudes is unprece...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thompson LG,Mosley-Thompson E,Brecher H,Davis M,León B,Les D,Lin PN,Mashiotta T,Mountain K

更新日期：2006-07-11 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopy provides a unique molecular fingerprint of tissue from endogenous sources of light absorption; however, specific molecular components of the overall FTIR signature of precancer have not been characterized. In attenuated total reflectance mode, infrared light penetrates on...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang TD,Triadafilopoulos G,Crawford JM,Dixon LR,Bhandari T,Sahbaie P,Friedland S,Soetikno R,Contag CH

更新日期：2007-10-02 00:00:00

abstract：:A power-law distribution of the length of perfectly conserved sequence from mouse/human whole-genome intersection and alignment is exhibited. Spatial correlations of these elements within the mouse genome are studied. It is argued that these power-law distributions and correlations are comprised in part by functional ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salerno W,Havlak P,Miller J

更新日期：2006-08-29 00:00:00

abstract：:Tissue- and cell-specific deletion of the Aqp4 gene is required to differentiate between the numerous pools of aquaporin-4 (AQP4) water channels. A glial-conditional Aqp4 knockout mouse line was generated to resolve whether astroglial AQP4 controls water exchange across the blood-brain interface. The conditional knock...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haj-Yasein NN,Vindedal GF,Eilert-Olsen M,Gundersen GA,Skare Ø,Laake P,Klungland A,Thorén AE,Burkhardt JM,Ottersen OP,Nagelhus EA

更新日期：2011-10-25 00:00:00

abstract：:Oncogenic mutations in the small GTPase KRAS are frequently found in human cancers, and, currently, there are no effective targeted therapies for these tumors. Using a combinatorial siRNA approach, we analyzed a panel of KRAS mutant colorectal and pancreatic cancer cell lines for their dependency on 28 gene nodes that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee CS,Lee LC,Yuan TL,Chakka S,Fellmann C,Lowe SW,Caplen NJ,McCormick F,Luo J

更新日期：2019-03-05 00:00:00

abstract：:Growth hormone (GH) has previously been implicated in T-cell development, and here we test its efficacy in promoting T-lymphocyte engraftment in mice with severe combined immune deficiency (SCID). SCID mice receiving syngeneic thymocytes and treated with recombinant human GH (rhGH) had significantly better T-cell engr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murphy WJ,Durum SK,Longo DL

更新日期：1992-05-15 00:00:00

abstract：:Rare high-3He/4He signatures in ocean island basalts (OIB) erupted at volcanic hotspots derive from deep-seated domains preserved in Earth's interior. Only high-3He/4He OIB exhibit anomalous 182W-an isotopic signature inherited during the earliest history of Earth-supporting an ancient origin of high 3He/4He. However,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jackson MG,Blichert-Toft J,Halldórsson SA,Mundl-Petermeier A,Bizimis M,Kurz MD,Price AA,Harðardóttir S,Willhite LN,Breddam K,Becker TW,Fischer RA

更新日期：2020-12-08 00:00:00

abstract：:The primate gastrointestinal tract is home to trillions of bacteria, whose composition is associated with numerous metabolic, autoimmune, and infectious human diseases. Although there is increasing evidence that modern and Westernized societies are associated with dramatic loss of natural human gut microbiome diversit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clayton JB,Vangay P,Huang H,Ward T,Hillmann BM,Al-Ghalith GA,Travis DA,Long HT,Tuan BV,Minh VV,Cabana F,Nadler T,Toddes B,Murphy T,Glander KE,Johnson TJ,Knights D

更新日期：2016-09-13 00:00:00

abstract：:We have compared the efficiency of transformation by linear DNA fragments in yeast strains carrying different numbers of homologous targets for recombination. In strains carrying dispersed copies of a target and in strains carrying tandem arrays, the frequency of transformation is proportional to the number of targets...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wilson JH,Leung WY,Bosco G,Dieu D,Haber JE

更新日期：1994-01-04 00:00:00

abstract：:Microinjection of progesterone dissolved in paraffin oil induces the reinitiation of meiotic maturation in the Xenopus oocyte; 50% maturation is obtained when 50 nl of a 50 microM solution is microinjected into the oocyte. The kinetics of the response to microinjected progesterone are similar to the kinetics of respon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tso J,Thibier C,Mulner O,Ozon R

更新日期：1982-09-01 00:00:00

abstract：:The enhancer-binding factor NF-kappa B, which is found only in cells that transcribe immunoglobulin light chain genes, has been purified from nuclear extracts of Namalwa cells (human Burkitt lymphoma cells) by sequence-specific DNA affinity chromatography. The purified NF-kappa B has been identified as a 51-kDa polype...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawakami K,Scheidereit C,Roeder RG

更新日期：1988-07-01 00:00:00

abstract：:Phospholipase Cε (PLCε) has been characterized as a direct effector of Ras in vitro and in cellular systems; however, the role of PLCε in tumorigenesis and its link to Ras in this context remain unclear. To assess the role of PLCε in Ras-driven cancers, we generated two new mouse strains: one carrying a targeted delet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Martins M,McCarthy A,Baxendale R,Guichard S,Magno L,Kessaris N,El-Bahrawy M,Yu P,Katan M

更新日期：2014-03-18 00:00:00

abstract：:Monovalent antibodies (Fab) directed to two classes of transformation-sensitive cell surface components, ganglioside and galactoprotein a (Gap a), inhibit the process of oncogenic viral transformation of cells. Mouse 3T3 cells infected with murine sarcoma virus were not transformed in terms of morphology change and en...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lingwood CA,Ng A,Hakomori S

更新日期：1978-12-01 00:00:00

abstract：:We studied regional cerebral blood flow using the H2(15)O method while normal subjects performed four similar tasks involving three-letter word beginnings (stems). Prior to each task, subjects studied a list of words. Local blood flow was then monitored during a 40-sec period while subjects (i) silently viewed word st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Squire LR,Ojemann JG,Miezin FM,Petersen SE,Videen TO,Raichle ME

更新日期：1992-03-01 00:00:00

abstract：:The late Pleistocene witnessed the extinction of 35 genera of North American mammals. The last appearance dates of 16 of these genera securely fall between 12,000 and 10,000 radiocarbon years ago (approximately 13,800-11,400 calendar years B.P.), although whether the absence of fossil occurrences for the remaining 19 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Faith JT,Surovell TA

更新日期：2009-12-08 00:00:00

abstract：:We have used a membrane-leak mass spectrometer to follow the time courses of H2 evolution and substrate reduction by nitrogenase [reduced ferredoxin:dinitrogen oxidoreductase (ATP-hydrolyzing), EC 1.18.6.1]. In the absence of added substrates, dinitrogenase passes all of its electrons to protons to form H2, but when a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang J,Burris RH

更新日期：1988-12-01 00:00:00

abstract：:Lactobacillus acidophilus NCFM is a probiotic bacterium that has been produced commercially since 1972. The complete genome is 1,993,564 nt and devoid of plasmids. The average GC content is 34.71% with 1,864 predicted ORFs, of which 72.5% were functionally classified. Nine phage-related integrases were predicted, but ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Altermann E,Russell WM,Azcarate-Peril MA,Barrangou R,Buck BL,McAuliffe O,Souther N,Dobson A,Duong T,Callanan M,Lick S,Hamrick A,Cano R,Klaenhammer TR

更新日期：2005-03-15 00:00:00

abstract：:We have studied the effects of recombinant (r) interleukin 7 (IL-7) on growth and differentiation of marrow pro-B-lymphocyte clones (CB/Bm7, LyD9, LyB9), marrow pro-T-lymphocyte clones (C4-77/3, C4-86/18, C4-95/16), and fetal thymocyte clones (FTH5, FTA2, FTD5) in the presence or absence of the bone marrow stroma clon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takeda S,Gillis S,Palacios R

更新日期：1989-03-01 00:00:00

abstract：:Iodine is an important nutrient and a significant sink of tropospheric ozone, a climate-forcing gas and air pollutant. Ozone interacts with seawater iodide, leading to volatile inorganic iodine release that likely represents the largest source of atmospheric iodine. Increasing ozone concentrations since the preindustr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Legrand M,McConnell JR,Preunkert S,Arienzo M,Chellman N,Gleason K,Sherwen T,Evans MJ,Carpenter LJ

更新日期：2018-11-27 00:00:00