Mammalian multidrug-resistance gene: correlation of exon organization with structural domains and duplication of an ancestral gene.

abstract：:The Paleocene-Eocene Thermal Maximum (PETM) (∼56 Ma) was a ∼170,000-y (∼170-kyr) period of global warming associated with rapid and massive injections of 13C-depleted carbon into the ocean-atmosphere system, reflected in sedimentary components as a negative carbon isotope excursion (CIE). Carbon cycle modeling has ind...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frieling J,Svensen HH,Planke S,Cramwinckel MJ,Selnes H,Sluijs A

更新日期：2016-10-25 00:00:00

abstract：:Cell fate determination depends in part on the establishment of specific transcriptional programs of gene expression. These programs result from the interpretation of the genomic cis-regulatory information by sequence-specific factors. Decoding this information in sequenced genomes is an important issue. Here, we deve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rouault H,Mazouni K,Couturier L,Hakim V,Schweisguth F

更新日期：2010-08-17 00:00:00

abstract：:The DNA polymerase enzymes from avian, murine, and feline RNA tumor viruses can be distinguished by their ability to read specific, synthetic primertemplates. The copying of templates containing adenylic and thymidylic acids by all these DNA polymerases is inhibited by ethidium bromide, though this compound affects th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fridlender B,Weissbach A

更新日期：1971-12-01 00:00:00

abstract：:The nature of integrated viral DNA in normal and leukemic chicken cells has been studied by sequential nucleic acid hybridization procedures that localize the viral specific DNA in cellular DNA regions differing in reiteration frequency. First, DNA.DNA reassociation was employed to fractionate cellular DNA sequences a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Evans RM,Baluda MA,Shoyab M

更新日期：1974-08-01 00:00:00

abstract：:High-affinity ligands of the reverse transcriptase of human immunodeficiency virus type 1 (HIV-1) were isolated by the SELEX procedure (systematic evolution of ligands by exponential enrichment) from RNA populations randomized at 32 positions. Analysis of these ligands revealed a pseudoknot consensus with primary sequ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tuerk C,MacDougal S,Gold L

更新日期：1992-08-01 00:00:00

abstract：:Understanding the function of high-density lipoprotein (HDL) requires detailed knowledge of the structure of its primary protein, apolipoprotein A-I (APOA1). However, APOA1 flexibility and HDL heterogeneity have confounded decades of efforts to determine high-resolution structures and consistent models. Here, molecula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pourmousa M,Song HD,He Y,Heinecke JW,Segrest JP,Pastor RW

更新日期：2018-05-15 00:00:00

abstract：:Mechanical injury to the adult mammalian spinal cord results in permanent loss of structural integrity at the lesion site and of the brain-controlled function distal to the lesion. Some of these consequences were permanently averted by altering the cellular constituents at the lesion site with x-irradiation delivered ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kalderon N,Fuks Z

更新日期：1996-10-01 00:00:00

abstract：:Thyroid hormone (TH) metabolism, mediated by deiodinase types 1, 2, and 3 (D1, D2, and D3) is profoundly affected by acute illness. We examined the role of TH metabolism during ventilator-induced lung injury (VILI) in mice. Mice exposed to VILI recapitulated the serum TH findings of acute illness, namely a decrease in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barca-Mayo O,Liao XH,DiCosmo C,Dumitrescu A,Moreno-Vinasco L,Wade MS,Sammani S,Mirzapoiazova T,Garcia JG,Refetoff S,Weiss RE

更新日期：2011-12-06 00:00:00

abstract：:The gene for cytosolic phosphoenolpyruvate carboxykinase (GTP) [GTP:oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.32] from the rat was isolated from a recombinant library containing the rat genome in phage lambda Charon 4A. The isolated clone, lambda PCK1, contains the complete gene for phosphoenolpyruva...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yoo-Warren H,Monahan JE,Short J,Short H,Bruzel A,Wynshaw-Boris A,Meisner HM,Samols D,Hanson RW

更新日期：1983-06-01 00:00:00

abstract：:It is widely accepted that modern humans originated in sub-Saharan Africa approximately 150-200 thousand years ago (ka), but their route of dispersal across the currently hyperarid Sahara remains controversial. Given that the first modern humans north of the Sahara are found in the Levant approximately 120-90 ka, nort...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Osborne AH,Vance D,Rohling EJ,Barton N,Rogerson M,Fello N

更新日期：2008-10-28 00:00:00

abstract：:Optical waveguides simultaneously transport light at different colors, forming the basis of fiber-optic telecommunication networks that shuttle data in dozens of spectrally separated channels. Here, we reimagine this wavelength division multiplexing (WDM) paradigm in a novel context--the differentiated detection and i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ozcelik D,Parks JW,Wall TA,Stott MA,Cai H,Parks JW,Hawkins AR,Schmidt H

更新日期：2015-10-20 00:00:00

abstract：:A temperature-sensitive ras allele has an effective lethal phase at 29 degrees C about 12 hr after pupation and a temperature-sensitive period for lethality beginning midway through the third larval instar and ending around pupation. The mutation also alters the quantity of pteridines present in the eyes, testes, and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grigliatti T,Suzuki DT

更新日期：1970-11-01 00:00:00

abstract：:An antigen histochemically localized in the nuclei and cytoplasmic granules of normal and leukemic human myeloid cells has been identified as myeloperoxidase (MPO; EC 1.11.1.7). The localization and amount of the enzyme was determined by using a murine monoclonal antibody designated H-43-5 raised against nuclear prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murao S,Stevens FJ,Ito A,Huberman E

更新日期：1988-02-01 00:00:00

abstract：:The effect of tumor promoters on electrical coupling between human FL cells was investigated with a microelectrode technique. When a low concentration (100 ng/ml) of 12-O-tetradecanoylphorbol 13-acetate (TPA) was added to culture medium, only 6% of the cells showed electrical coupling after 5 hr, whereas in control me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Enomoto T,Sasaki Y,Shiba Y,Kanno Y,Yamasaki H

更新日期：1981-09-01 00:00:00

abstract：:The actin cytoskeleton is a critical regulator of cytoplasmic architecture and mechanics, essential in a myriad of physiological processes. Here we demonstrate a liquid phase of actin filaments in the presence of the physiological cross-linker, filamin. Filamin condenses short actin filaments into spindle-shaped dropl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weirich KL,Banerjee S,Dasbiswas K,Witten TA,Vaikuntanathan S,Gardel ML

更新日期：2017-02-28 00:00:00

abstract：:Flowering time (FT) is the developmental transition coupling an internal genetic program with external local and seasonal climate cues. The genetic loci sensitive to predictable environmental signals underlie local adaptation. We dissected natural variation in FT across a new global diversity set of 473 unique accessi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Y,Huang Y,Bergelson J,Nordborg M,Borevitz JO

更新日期：2010-12-07 00:00:00

abstract：:Profiling the dynamic interaction of p300 with proximal promoters of human T cells identified a class of genes that rapidly coassemble p300 and RNA polymerase II (pol II) following mitogen stimulation. Several of these p300 targets are immediate early genes, including FOS, implicating a prominent role for p300 in the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Byun JS,Wong MM,Cui W,Idelman G,Li Q,De Siervi A,Bilke S,Haggerty CM,Player A,Wang YH,Thirman MJ,Kaberlein JJ,Petrovas C,Koup RA,Longo D,Ozato K,Gardner K

更新日期：2009-11-17 00:00:00

abstract：:Adult rats were trained to detect the occurrence of a two-element sound sequence in a background of nine other nontarget sound pairs. Training resulted in a modest, enduring, static expansion of the cortical areas of representation of both target stimulus sounds. More importantly, once the initial stimulus A in the ta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou X,de Villers-Sidani E,Panizzutti R,Merzenich MM

更新日期：2010-08-17 00:00:00

abstract：:Six Rhesus monkeys between 24 and 29 years of age received unilateral transections of the fornix. Three monkeys then received intraventricular transplants of polymer-encapsulated baby hamster kidney (BHK) fibroblasts that had been genetically modified to secrete human nerve growth factor (hNGF). The remaining three mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kordower JH,Winn SR,Liu YT,Mufson EJ,Sladek JR Jr,Hammang JP,Baetge EE,Emerich DF

更新日期：1994-11-08 00:00:00

abstract：:Chronic microbial infections are associated with fibrotic and inflammatory reactions known as granulomas showing similarities to wound-healing and tissue repair processes. We have previously mapped three leishmaniasis susceptibility loci, designated lmr1, -2, and -3, which exert their effect independently of T cell im...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sakthianandeswaren A,Elso CM,Simpson K,Curtis JM,Kumar B,Speed TP,Handman E,Foote SJ

更新日期：2005-10-25 00:00:00

abstract：:Translation elongation factor P (EF-P) stimulates ribosomal peptidyltransferase activity. EF-P is conserved in bacteria and is essential for cell viability. Eukarya and Archaea have an EF-P homologue, eukaryotic initiation factor 5A (eIF-5A). In the present study, we determined the crystal structure of EF-P from Therm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hanawa-Suetsugu K,Sekine S,Sakai H,Hori-Takemoto C,Terada T,Unzai S,Tame JR,Kuramitsu S,Shirouzu M,Yokoyama S

更新日期：2004-06-29 00:00:00

abstract：:Spontaneous cross-linking in vitro, during incubation of purified collagen in nonstriated and native type fibrils, was studied to elucidate the relationship between intra and intermolecular cross-links. In nonbanded fibrils, the aldol condensation products of two allysyl residues, previously shown to constitute the in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang AH,Gross J

更新日期：1970-11-01 00:00:00

abstract：:Endocytic protein trafficking is directed by sorting signals on cargo molecules that are recognized by cytosolic adaptor proteins. However, the steps necessary to segregate the variety of cargoes during endocytosis remain poorly defined. Using Caenorhabditis elegans, we demonstrate that multiple plasma membrane endocy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mayers JR,Wang L,Pramanik J,Johnson A,Sarkeshik A,Wang Y,Saengsawang W,Yates JR 3rd,Audhya A

更新日期：2013-07-16 00:00:00

abstract：:U2 snRNP auxiliary factor 65 kDa (U2AF(65)) is a general splicing factor that contacts polypyrimidine (Py) tract and promotes prespliceosome assembly. In this report, we show that U2AF(65) stimulates alternative exon skipping in spinal muscular atrophy (SMA)-related survival motor neuron (SMN) pre-mRNA. A stronger 5' ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cho S,Moon H,Loh TJ,Jang HN,Liu Y,Zhou J,Ohn T,Zheng X,Shen H

更新日期：2015-08-11 00:00:00

abstract：:We have asked here how the remarkable variation in maize haplotype structure affects recombination. We compared recombination across a genetic interval of 9S in 2 highly dissimilar heterozygotes that shared 1 parent. The genetic interval in the common haplotype is approximately 100 kb long and contains 6 genes intersp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He L,Dooner HK

更新日期：2009-05-26 00:00:00

abstract：:A method is presented for variational calculation of the energy and the spin densities derived from a single-determinant wavefunction. Sum and difference coordinates [unk]R = (1/2)([unk]r(1) + [unk]r(1)) and [unk]r = [unk]r(1) - [unk]r(1) are introduced, and the density matrix P([unk]r(1),[unk]r(1)) is expanded in par...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Payne PW

更新日期：1980-11-01 00:00:00

abstract：:It is possible, by using Fourier-transform infrared (FTIR) difference spectroscopy, to detect the conformational changes occurring in both the protein and the chromophore of bacteriorhodopsin during the photocycle. In contrast to Raman spectroscopy, a laser is unnecessary and hence the problem of a perturbing probe be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rothschild KJ,Marrero H

更新日期：1982-07-01 00:00:00

abstract：:Infectious RNA transcripts were generated from full-length cDNA clones of the tobacco etch potyvirus genome containing an insertion of the bacterial beta-glucuronidase (GUS) gene between the polyprotein-coding sequences for the N-terminal 35-kDa proteinase and the helper component-proteinase. The recombinant virus was...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dolja VV,McBride HJ,Carrington JC

更新日期：1992-11-01 00:00:00

abstract：:Using [3H]quinuclidinyl benzilate as a specific cholinergic muscarinic ligand, it has been demonstrated that lymphocytes have muscarinic binding sites. There are approximately 200 sites per cell and the dissociation constant for quinuclidinyl benzilate is approximately 1 X 10(-9) M. Quinuclidinyl benzilate receptor bi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon MA,Cohen JJ,Wilson IB

更新日期：1978-06-01 00:00:00

abstract：:Dyskeratosis congenita is a premature aging syndrome characterized by muco-cutaneous features and a range of other abnormalities, including early greying, dental loss, osteoporosis, and malignancy. Dyskeratosis congenita cells age prematurely and have very short telomeres. Patients have mutations in genes that encode ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vulliamy T,Beswick R,Kirwan M,Marrone A,Digweed M,Walne A,Dokal I

更新日期：2008-06-10 00:00:00