Abstract:
:Optical waveguides simultaneously transport light at different colors, forming the basis of fiber-optic telecommunication networks that shuttle data in dozens of spectrally separated channels. Here, we reimagine this wavelength division multiplexing (WDM) paradigm in a novel context--the differentiated detection and identification of single influenza viruses on a chip. We use a single multimode interference (MMI) waveguide to create wavelength-dependent spot patterns across the entire visible spectrum and enable multiplexed single biomolecule detection on an optofluidic chip. Each target is identified by its time-dependent fluorescence signal without the need for spectral demultiplexing upon detection. We demonstrate detection of individual fluorescently labeled virus particles of three influenza A subtypes in two implementations: labeling of each virus using three different colors and two-color combinatorial labeling. By extending combinatorial multiplexing to three or more colors, MMI-based WDM provides the multiplexing power required for differentiated clinical tests and the growing field of personalized medicine.
journal_name
Proc Natl Acad Sci U S Aauthors
Ozcelik D,Parks JW,Wall TA,Stott MA,Cai H,Parks JW,Hawkins AR,Schmidt Hdoi
10.1073/pnas.1511921112subject
Has Abstractpub_date
2015-10-20 00:00:00pages
12933-7issue
42eissn
0027-8424issn
1091-6490pii
1511921112journal_volume
112pub_type
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