Abstract:
:The enhancer-binding factor NF-kappa B, which is found only in cells that transcribe immunoglobulin light chain genes, has been purified from nuclear extracts of Namalwa cells (human Burkitt lymphoma cells) by sequence-specific DNA affinity chromatography. The purified NF-kappa B has been identified as a 51-kDa polypeptide by UV-crosslinking analysis. "Footprint" and methylation-interference analyses have shown that purified NF-kappa B has a binding activity specific for the kappa light chain enhancer sequence. The purified factor activated in vitro transcription of the human immunodeficiency virus type I promoter by binding to an upstream NF-kappa B-binding site.
journal_name
Proc Natl Acad Sci U S Aauthors
Kawakami K,Scheidereit C,Roeder RGdoi
10.1073/pnas.85.13.4700subject
Has Abstractpub_date
1988-07-01 00:00:00pages
4700-4issue
13eissn
0027-8424issn
1091-6490journal_volume
85pub_type
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