Hydrogen burst associated with nitrogenase-catalyzed reactions.

abstract：:The nusB5 mutant of Escherichia coli was originally selected for reduced ability to support the antitermination of transcription that is mediated by the gene N product of bacteriophage lambda. By analyzing pulse-labeled RNA with an RNA.DNA filter hybridization technique, we have shown that, in the nusB5 mutant, the ra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharrock RA,Gourse RL,Nomura M

更新日期：1985-08-01 00:00:00

abstract：:Synapsin I is a neuron-specific phosphoprotein associated with the membranes of small synaptic vesicles. Its function is not entirely clear, but evidence points to a possible role in the regulation of neurotransmitter release. Its biosynthesis is under developmental control. Assignment of the human synapsin I gene to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang-Feng TL,DeGennaro LJ,Francke U

更新日期：1986-11-01 00:00:00

abstract：:Polyamides containing N-methylimidazole (Im) and N-methylpyrrole (Py) amino acids can be combined in antiparallel side-by-side dimeric complexes for sequence-specific recognition in the minor groove of DNA. Six polyamides containing three to eight rings bind DNA sites 5-10 bp in length, respectively. Quantitative DNas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelly JJ,Baird EE,Dervan PB

更新日期：1996-07-09 00:00:00

abstract：:Many marine bacteria have evolved to grow optimally at either high (copiotrophic) or low (oligotrophic) nutrient concentrations, enabling different species to colonize distinct trophic habitats in the oceans. Here, we compare the genome sequences of two bacteria, Photobacterium angustum S14 and Sphingopyxis alaskensis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lauro FM,McDougald D,Thomas T,Williams TJ,Egan S,Rice S,DeMaere MZ,Ting L,Ertan H,Johnson J,Ferriera S,Lapidus A,Anderson I,Kyrpides N,Munk AC,Detter C,Han CS,Brown MV,Robb FT,Kjelleberg S,Cavicchioli R

更新日期：2009-09-15 00:00:00

abstract：:Barth, Larsen, and others showed that complex submanifolds of complex projective space, CP(N), of small codimension strongly resemble CP(N) both homotopically and cohomologically. These results are generalized to yield analogous results for complex subspaces of arbitrary homogeneous complex manifolds. One very special...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sommese AJ

更新日期：1977-04-01 00:00:00

abstract：:Somatic mutations can now be quantified in almost any cell type in mice carrying bacterial genes in a lambda phage shuttle vector. Mutations induced in vivo are detectable ex vivo, after packaging host-cell DNA into phage that are grown on suitable bacteria. However, the transgenic DNA differs from many host loci in s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tao KS,Urlando C,Heddle JA

更新日期：1993-11-15 00:00:00

abstract：:In oxygenic photosynthetic eukaryotes, the hydroxylated carotenoid zeaxanthin is produced from preexisting violaxanthin upon exposure to excess light conditions. Zeaxanthin binding to components of the photosystem II (PSII) antenna system has been investigated thoroughly and shown to help in the dissipation of excess ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ballottari M,Alcocer MJ,D'Andrea C,Viola D,Ahn TK,Petrozza A,Polli D,Fleming GR,Cerullo G,Bassi R

更新日期：2014-06-10 00:00:00

abstract：:Bone morphogenetic protein 2 (BMP-2) is a potent inducer of differentiation of osteoblasts both in vivo and in vitro. We examined the action of BMP-2 on expression of a helix-loop-helix-type transcription factor, Id (inhibitor of differentiation), in osteoblast-like cells, as well as in osteoblast-enriched cells and p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ogata T,Wozney JM,Benezra R,Noda M

更新日期：1993-10-01 00:00:00

abstract：:Our purpose is to investigate the feasibility of imaging tumor metabolism in breast cancer patients using 13C magnetic resonance spectroscopic imaging (MRSI) of hyperpolarized 13C label exchange between injected [1-13C]pyruvate and the endogenous tumor lactate pool. Treatment-naïve breast cancer patients were recruite...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gallagher FA,Woitek R,McLean MA,Gill AB,Manzano Garcia R,Provenzano E,Riemer F,Kaggie J,Chhabra A,Ursprung S,Grist JT,Daniels CJ,Zaccagna F,Laurent MC,Locke M,Hilborne S,Frary A,Torheim T,Boursnell C,Schiller A,Pa

更新日期：2020-01-28 00:00:00

abstract：:A central tenet of evolutionary social science holds that behaviors, such as those associated with social dominance, produce fitness effects that are subject to cultural selection. However, evidence for such selection is inconclusive because it is based on short-term statistical associations between behavior and ferti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lansing JS,Watkins JC,Hallmark B,Cox MP,Karafet TM,Sudoyo H,Hammer MF

更新日期：2008-08-19 00:00:00

abstract：:Cellular communities in living tissues act in concert to establish intricate microenvironments, with complexity difficult to recapitulate in vitro. We report a method for docking numerous cellularized hydrogel shapes (100-1,000 µm in size) into hydrogel templates to construct 3D cellular microenvironments. Each shape ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eng G,Lee BW,Parsa H,Chin CD,Schneider J,Linkov G,Sia SK,Vunjak-Novakovic G

更新日期：2013-03-19 00:00:00

abstract：:Kabuki syndrome is a Mendelian intellectual disability syndrome caused by mutations in either of two genes (KMT2D and KDM6A) involved in chromatin accessibility. We previously showed that an agent that promotes chromatin opening, the histone deacetylase inhibitor (HDACi) AR-42, ameliorates the deficiency of adult neur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benjamin JS,Pilarowski GO,Carosso GA,Zhang L,Huso DL,Goff LA,Vernon HJ,Hansen KD,Bjornsson HT

更新日期：2017-01-03 00:00:00

abstract：:Efforts to increase the potency of transcriptional activators are generally unsuccessful because poor expression of activators in mammalian cells limits their delivery to target promoters. Here we report that the effectiveness of chimeric activators can be dramatically improved by expressing them as noncovalent tetram...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Natesan S,Molinari E,Rivera VM,Rickles RJ,Gilman M

更新日期：1999-11-23 00:00:00

abstract：:The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47-->methionine mutant proteins with x-ray diffraction data phased to 2.5 A resolution. The structure of the wild-type protein h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skinner MM,Zhang H,Leschnitzer DH,Guan Y,Bellamy H,Sweet RM,Gray CW,Konings RN,Wang AH,Terwilliger TC

更新日期：1994-03-15 00:00:00

abstract：:The use of computers to simulate the functions of complex biological macromolecules is essential to achieve a microscopic description of biological processes and to model and interpret experimental data. Here we apply theoretical computational approaches to investigate the fidelity of T7 DNA polymerase, divided into d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Florián J,Goodman MF,Warshel A

更新日期：2005-05-10 00:00:00

abstract：:Preaxial polydactyly (PPD) is a common limb malformation in human. A number of polydactylous mouse mutants indicate that misexpression of Shh is a common requirement for generating extra digits. Here we identify a translocation breakpoint in a PPD patient and a transgenic insertion site in the polydactylous mouse muta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lettice LA,Horikoshi T,Heaney SJ,van Baren MJ,van der Linde HC,Breedveld GJ,Joosse M,Akarsu N,Oostra BA,Endo N,Shibata M,Suzuki M,Takahashi E,Shinka T,Nakahori Y,Ayusawa D,Nakabayashi K,Scherer SW,Heutink P,Hill RE

更新日期：2002-05-28 00:00:00

abstract：:To determine the importance of mitochondrial reactive oxygen species toxicity in aging and senescence, we analyzed changes in mitochondrial function with age in mice with partial or complete deficiencies in the mitochondrial antioxidant enzyme manganese superoxide dismutase (MnSOD). Liver mitochondria from homozygous ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kokoszka JE,Coskun P,Esposito LA,Wallace DC

更新日期：2001-02-27 00:00:00

abstract：:The coat protein of bacteriophage MS-2 was cleaved with cyanogen bromide to yield three fragments, possessing the sequence 1-88 (P1), 89-108 (P2), and 109-129 (P3), respectively. The mixture of peptides P2 and P3, which could not be separated, was found capable of inhibiting the neutralization of the phage by antiseru...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Langbeheim H,Arnon R,Sela M

更新日期：1976-12-01 00:00:00

abstract：:X chromosome-linked severe combined immunodeficiency disease (SCIDX1) is characterized by the absence of T-cell and natural killer cell development and results from molecular mutations of the interleukin 2 receptor (IL-2R) gamma chain. The IL-2R gamma chain is a common component of the IL-2, IL-4, and IL-7 receptor sy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DiSanto JP,Rieux-Laucat F,Dautry-Varsat A,Fischer A,de Saint Basile G

更新日期：1994-09-27 00:00:00

abstract：:We investigate the dynamics and spatial distribution of land use fragmentation in a rapidly urbanizing region of the United States to test key propositions regarding the evolution of sprawl. Using selected pattern metrics and data from 1973 and 2000 for the state of Maryland, we find significant increases in developed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Irwin EG,Bockstael NE

更新日期：2007-12-26 00:00:00

abstract：:Several cDNA clones encoding the human immunoglobulin G receptor CD16 were isolated from human lung or peripheral blood leukocyte cDNA libraries. Nucleotide sequence comparisons revealed that the cDNAs could be divided into two groups. cDNA clones in one group encode a protein that terminates 4 amino acids after the p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scallon BJ,Scigliano E,Freedman VH,Miedel MC,Pan YC,Unkeless JC,Kochan JP

更新日期：1989-07-01 00:00:00

abstract：:The role of the cerebellar cortex in motor learning was investigated by comparing the paramedian lobule of adult rats given difficult acrobatic training to that of rats that had been given extensive physical exercise or had been inactive. The paramedian lobule is activated during limb movements used in both acrobatic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Black JE,Isaacs KR,Anderson BJ,Alcantara AA,Greenough WT

更新日期：1990-07-01 00:00:00

abstract：:A sensitive target binding assay has recently been shown to detect natural killer (NK) cells in the mouse. Preincubation of NK cells with detergent-solubilized cell-surface proteins of YAC lymphoma cells prevented subsequent binding to intact YAC targets. The NK target structures (NK-TS) consisted of three molecular s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roder JC,Rosén A,Fenyö EM,Troy FA

更新日期：1979-03-01 00:00:00

abstract：:Multiple paleoclimate proxies are required for robust assessment of past hydroclimatic conditions. Currently, estimates of drought variability over the past several thousand years are based largely on tree-ring records. We produced a 1,500-y record of winter precipitation in the Pacific Northwest using a physical mode...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Steinman BA,Abbott MB,Mann ME,Stansell ND,Finney BP

更新日期：2012-07-17 00:00:00

abstract：:DNA polymerases (DNAPs) responsible for genome replication are highly faithful enzymes that nonetheless cannot deal with damaged DNA. In contrast, translesion synthesis (TLS) DNAPs are suitable for replicating modified template bases, although resulting in very low-fidelity products. Here we report the biochemical cha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berjón-Otero M,Villar L,de Vega M,Salas M,Redrejo-Rodríguez M

更新日期：2015-07-07 00:00:00

abstract：:The Per-Arnt-Sim (PAS) domain is a ubiquitous protein module with a common three-dimensional fold involved in a wide range of regulatory and sensory functions in all domains of life. The activation of these functions is thought to involve partial unfolding of N- or C-terminal helices attached to the PAS domain. Here w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao JM,Lee H,Nome RA,Majid S,Scherer NF,Hoff WD

更新日期：2006-08-01 00:00:00

abstract：:Understanding modulation of memory, as well as the mechanisms underlying memory formation, has become a key issue in neuroscience research. Previously, we found that the formation of long-term, but not short-term, memory for a nonassociative form of learning, sensitization, was modulated by the circadian clock in the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lyons LC,Rawashdeh O,Katzoff A,Susswein AJ,Eskin A

更新日期：2005-08-30 00:00:00

abstract：:Biologically active (125)I-labeled bovine parathyroid hormone (prepared by electrolytic iodination) and its synthetic NH(2)-terminal (1-34) biologically active fragment bound rapidly and specifically to a purified plasma membrane preparation from bovine renal cortex. Binding of labeled intact hormone or labeled NH(2)-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Di Bella FP,Dousa TP,Miller SS,Arnaud CD

更新日期：1974-03-01 00:00:00

abstract：:The estrogen receptor (E2R) from rabbit uterus has an enhanced affinity for BrdUrd-substituted DNA compared to unsubstituted DNA. Increasing levels of BrdUrd substitution (from 0 to 100%) in a DNA sample are associated with increasingly tighter binding of E2R to that DNA as measured by equilibrium competition experime...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kallos J,Fasy TM,Hollander VP,Bick MD

更新日期：1978-10-01 00:00:00

abstract：:A five-helix secondary structural model is proposed for eukaryotic cytoplasmic 5S rRNA. All available sequence data are consistent with this model including those from Chlorella 5S rRNA whose sequence is revised by data included here. Various architectural features of eukaryotic 5S rRNA are summarized in terms of this...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luehrsen KR,Fox GE

更新日期：1981-04-01 00:00:00