Abstract:
:We have purified a protein from Necturus maculosus gallbladder cells that forms chloride channels in an artificial membrane. The same protein apparently can form channels that are highly selective for chloride but can have conductances varying from 9 to about 150 pS. The high-conductance channels are blocked by the monoclonal antibody used to purify the protein, but this antibody has no effect on the 9-pS channels. The observation that gating of the low- and high-conductance states is independent and that the antibody affects only the latter has implications regarding the control of chloride conductance in cell membranes and the different types of channels described in those cells.
journal_name
Proc Natl Acad Sci U S Aauthors
Finn AL,Dillard M,Gaido Mdoi
10.1073/pnas.90.12.5691subject
Has Abstractpub_date
1993-06-15 00:00:00pages
5691-4issue
12eissn
0027-8424issn
1091-6490journal_volume
90pub_type
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