Quantitative expression profiling of identified neurons reveals cell-specific constraints on highly variable levels of gene expression.

abstract：:Serotonin (5-HT) controls a wide range of biological functions. In the brain, its implication as a neurotransmitter and in the control of behavioral traits has been largely documented. At the periphery, its modulatory role in physiological processes, such as the cardiovascular function, is still poorly understood. The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Côté F,Thévenot E,Fligny C,Fromes Y,Darmon M,Ripoche MA,Bayard E,Hanoun N,Saurini F,Lechat P,Dandolo L,Hamon M,Mallet J,Vodjdani G

更新日期：2003-11-11 00:00:00

abstract：:Olfactory neuroblastoma (ONB) is a malignant tumor of the nasal mucosa whose histogenesis is unclear. A relationship to neuroblastoma (NB), a pediatric tumor of the sympathetic nervous system, is based on morphologic similarities and the expression of similar neural antigens. However, the clinical presentation of ONB ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sorensen PH,Wu JK,Berean KW,Lim JF,Donn W,Frierson HF,Reynolds CP,López-Terrada D,Triche TJ

更新日期：1996-02-06 00:00:00

abstract：:A systematic comparison of crystal structures of nine different B-DNA dodecamers, in three different space groups, with and without A-tracts, shows that crystal packing or lattice forces are of secondary importance for helix axis bending, minor-groove width, and propeller twist. While other local helix parameters may ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dickerson RE,Goodsell DS,Neidle S

更新日期：1994-04-26 00:00:00

abstract：:CD4 can physically associate with the CD3-T-cell receptor complex as visualized in cocapping experiments. This association occurs when the T-cell receptor is cross-linked by certain anti-variable region antibodies that appear to induce a conformational change in the receptor such that it associate with CD4. Similar as...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rojo JM,Saizawa K,Janeway CA Jr

更新日期：1989-05-01 00:00:00

abstract：:Ethylenediaminetetraacetic acid and diethylenetriaminepentaacetic acid have been attached covalently to the 5' terminus of the deoxynucleotide sequence C-A-C-A-A-T-T-C-C-A-C-A-C-A-A-C (16-mer) via an ethylenediamine linker. In the presence of Fe2+ and dithiothreitol, these reagents bring about the hybridization-depend...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chu BC,Orgel LE

更新日期：1985-02-01 00:00:00

abstract：:The hypothesis that intracellular Ca2+ is an essential component of the intracellular mechanism of insulin action in the adipocyte was evaluated. Cells were loaded with the Ca2+ chelator quin-2, by preincubating them with quin-2 AM, the tetrakis(acetoxymethyl) ester of quin-2. Quin-2 loading inhibited insulin-stimulat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pershadsingh HA,Shade DL,Delfert DM,McDonald JM

更新日期：1987-02-01 00:00:00

abstract：:Oligothiophenes incorporating MM quadruple bonds have been prepared from the reactions between Mo(2)(TiPB)(4) (TiPB = 2,4,6-triisopropyl benzoate) and 3',4'-dihexyl-2,2'-:5',2''-terthiophene-5,5''-dicarboxylic acid. The oligomers of empirical formula Mo(2)(TiPB)(2)(O(2)C(Th)-C(4)(n-hexyl)(2)S-(Th)CO(2)) are soluble in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burdzinski GT,Chisholm MH,Chou PT,Chou YH,Feil F,Gallucci JC,Ghosh Y,Gustafson TL,Ho ML,Liu Y,Ramnauth R,Turro C

更新日期：2008-10-07 00:00:00

abstract：:A new type of self-assembling peptide (sapeptide) scaffolds that serve as substrates for neurite outgrowth and synapse formation is described. These peptide-based scaffolds are amenable to molecular design by using chemical or biotechnological syntheses. They can be tailored to a variety of applications. The sapeptide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holmes TC,de Lacalle S,Su X,Liu G,Rich A,Zhang S

更新日期：2000-06-06 00:00:00

abstract：:Cell membrane contact induces the de novo expression of choline O-acetyltransferase (CAT; acetyl-CoA: choline O-acetyltransferase, EC 2.3.1.6) activity in cultures of virtually pure neonatal rat dissociated sympathetic neurons. To identify molecular mechanisms underlying membrane-associated CAT induction, the responsi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adler JE,Schleifer LS,Black IB

更新日期：1989-02-01 00:00:00

abstract：:Prior incubation of quiescent cultures of Swiss 3T3 cells with vasopressin leads to loss of mitogenic stimulation on its subsequent addition in the presence of a synergistic growth factor. This desensitization is selective for vasopressin, requires prolonged incubation (half-maximal desensitization after 12 hr of trea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collins MK,Rozengurt E

更新日期：1983-04-01 00:00:00

abstract：:Platelet-derived growth factors (PDGFs) and their receptors (PDGFRs) are prototypic growth factors and receptor tyrosine kinases which have critical functions in development. We show that PDGFs share a conserved region in their prodomain sequences which can remain noncovalently associated with the mature cystine-knot ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shim AH,Liu H,Focia PJ,Chen X,Lin PC,He X

更新日期：2010-06-22 00:00:00

abstract：:Infants born prematurely risk significant life-long cognitive disability, representing a major pediatric health crisis. The neuropathology of this cohort is accurately modeled in mice subjected to sublethal postnatal hypoxia. Massively parallel transcriptome analysis using cDNA microchips (9,262 genes), combined with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Curristin SM,Cao A,Stewart WB,Zhang H,Madri JA,Morrow JS,Ment LR

更新日期：2002-11-26 00:00:00

abstract：:A census of the biomass on Earth is key for understanding the structure and dynamics of the biosphere. However, a global, quantitative view of how the biomass of different taxa compare with one another is still lacking. Here, we assemble the overall biomass composition of the biosphere, establishing a census of the ≈5...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bar-On YM,Phillips R,Milo R

更新日期：2018-06-19 00:00:00

abstract：:The trilaminate vascular architecture provides biochemical regulation and mechanical integrity. Yet regulatory control can be regained after injury without recapitulating tertiary structure. Tissue-engineered (TE) endothelium controls repair even when placed in the perivascular space of injured vessels. It remains unc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zani BG,Kojima K,Vacanti CA,Edelman ER

更新日期：2008-05-13 00:00:00

abstract：:Kinetics of growth and decay of triplet states of molecules at known orientation in a magnetic field have been measured. Amplitudes of the zero field eigenstates in each of the eigenstates at finite field were varied by choice of orientation. No interference between the amplitudes of the zero field states was found in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Felix CC,Weissman SI

更新日期：1975-11-01 00:00:00

abstract：:Saccharomyces cerevisiae translation initiation factor eIF-4A, an RNA helicase of the Asp-Glu-Ala-Asp (DEAD) box protein family, was mutated in the putative ATP binding site and expressed in Escherichia coli. Mutant proteins with alanine at position 66 replaced by glycine [eIF-4A(A66G)] or valine [eIF-4A(A66V)] were p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blum S,Schmid SR,Pause A,Buser P,Linder P,Sonenberg N,Trachsel H

更新日期：1992-08-15 00:00:00

abstract：:Human rhinovirus (HRV) and other members of the enterovirus genus bind small-molecule antiviral compounds in a cavity buried within the viral capsid protein VP1. These compounds block the release of the viral protein VP4 and RNA from inside the capsid during the uncoating process. In addition, the antiviral compounds ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roy A,Post CB

更新日期：2012-04-03 00:00:00

abstract：:A small proportion of the RNAs of mouse reticulocytes consists of beta major-globin mRNA sequences linked to sequences transcribed from the 5'-flanking region of the beta major-globin gene. These upstream RNAs are polyadenylylated and contain 700-800 nucleotides, and their 5' regions are heterogeneous. RNAs with simil...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carlson DP,Ross J

更新日期：1984-12-01 00:00:00

abstract：:We have detected a variant species of bovine growth hormone mRNA in bovine pituitary tissue and in a stably transfected bovine growth hormone-producing cell line. Analysis of this variant mRNA indicated that the last intervening sequence (intron D) had not been removed by splicing. Inspection of the sequence of intron...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hampson RK,Rottman FM

更新日期：1987-05-01 00:00:00

abstract：:Natively unfolded proteins play key roles in normal and pathological biochemical processes. Despite their importance for function, this category of proteins remains beyond the reach of classical structural biology because of their inherent conformational heterogeneity. We present a description of the intrinsic conform...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bernadó P,Blanchard L,Timmins P,Marion D,Ruigrok RW,Blackledge M

更新日期：2005-11-22 00:00:00

abstract：:When either the homologous RNA (avian myeloblastosis virus RNA) or a heterologous RNA (poliovirus RNA) was used as a template, the anticomplementary DNA synthesized in vitro by avian myeloblastosis virus reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) was primed by fragments of the original...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Myers JC,Dobkin C,Spiegelman S

更新日期：1980-03-01 00:00:00

abstract：:Pulmonary surfactant contains two hydrophobic polypeptides, SP-B and SP-C, with known amino acid sequences and with truncated subforms lacking the N-terminal residues. Treatment of SP-C with KOH releases fatty acids (palmitic acid to more than 85%) in molar ratios of 1.8-2.0 relative to the polypeptide. Furthermore, p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Curstedt T,Johansson J,Persson P,Eklund A,Robertson B,Löwenadler B,Jörnvall H

更新日期：1990-04-01 00:00:00

abstract：:The tissue-specific transcriptional coactivator OCA-B is required for antigen-dependent B cell differentiation events, including germinal center formation. However, the identity of OCA-B target genes involved in this process is unknown. This study has used large-scale cDNA arrays to monitor changes in gene expression ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim U,Siegel R,Ren X,Gunther CS,Gaasterland T,Roeder RG

更新日期：2003-07-22 00:00:00

abstract：:Sustained activity of mammalian methionine synthase (MS) requires MS reductase (MSR), but there have been few studies of the interactions between these two proteins. In this study, recombinant human MS (hMS) and MSR (hMSR) were expressed in baculovirus-infected insect cells and purified to homogeneity. hMSR maintained...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamada K,Gravel RA,Toraya T,Matthews RG

更新日期：2006-06-20 00:00:00

abstract：:Understanding the mechanisms of Salmonella virulence is an important challenge. The capacity of this intracellular bacterial pathogen to cause diseases depends on the expression of virulence factors including the second type III secretion system (TTSS-2), which is used to translocate into the eukaryotic cytosol a set ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henry T,Couillault C,Rockenfeller P,Boucrot E,Dumont A,Schroeder N,Hermant A,Knodler LA,Lecine P,Steele-Mortimer O,Borg JP,Gorvel JP,Méresse S

更新日期：2006-09-05 00:00:00

abstract：:Cyanobacteria are phototrophic prokaryotes that evolved oxygenic photosynthesis ∼2.7 billion y ago and are presently responsible for ∼10% of total global photosynthetic production. To cope with the evolutionary pressure of dropping ambient CO2 concentrations, they evolved a CO2-concentrating mechanism (CCM) to augment...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Selim KA,Haase F,Hartmann MD,Hagemann M,Forchhammer K

更新日期：2018-05-22 00:00:00

abstract：:Microorganisms have mechanisms to sense their environment and rapidly adapt to survive changes in conditions. In Streptomyces albus, various transcriptional repressors mediate the induction of heat shock genes. The RheA repressor regulates the synthesis of HSP18, a small heat shock protein, which plays a role in therm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Servant P,Grandvalet C,Mazodier P

更新日期：2000-03-28 00:00:00

abstract：:Carbohydrate-based vaccines have shown therapeutic efficacy for infectious disease and cancer. The mushroom Ganoderma lucidum (Reishi) containing complex polysaccharides has been used as antitumor supplement, but the mechanism of immune response has rarely been studied. Here, we show that the mice immunized with a l-f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liao SF,Liang CH,Ho MY,Hsu TL,Tsai TI,Hsieh YS,Tsai CM,Li ST,Cheng YY,Tsao SM,Lin TY,Lin ZY,Yang WB,Ren CT,Lin KI,Khoo KH,Lin CH,Hsu HY,Wu CY,Wong CH

更新日期：2013-08-20 00:00:00

abstract：:Paleogenetics is an emerging field that resurrects ancestral proteins from now-extinct organisms to test, in the laboratory, models of protein function based on natural history and Darwinian evolution. Here, we resurrect digestive alcohol dehydrogenases (ADH4) from our primate ancestors to explore the history of prima...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carrigan MA,Uryasev O,Frye CB,Eckman BL,Myers CR,Hurley TD,Benner SA

更新日期：2015-01-13 00:00:00

abstract：:I reject the Darwinian assumption that larvae and their adults evolved from a single common ancestor. Rather I posit that, in animals that metamorphose, the basic types of larvae originated as adults of different lineages, i.e., larvae were transferred when, through hybridization, their genomes were acquired by distan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williamson DI

更新日期：2009-11-24 00:00:00