当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Significance analysis of xMap cytokine bead arrays.

Significance analysis of xMap cytokine bead arrays.

Abstract：

:Highly multiplexed assays using antibody coated, fluorescent (xMap) beads are widely used to measure quantities of soluble analytes, such as cytokines and antibodies in clinical and other studies. Current analyses of these assays use methods based on standard curves that have limitations in detecting low or high abundance analytes. Here we describe SAxCyB (Significance Analysis of xMap Cytokine Beads), a method that uses fluorescence measurements of individual beads to find significant differences between experimental conditions. We show that SAxCyB outperforms conventional analysis schemes in both sensitivity (low fluorescence) and robustness (high variability) and has enabled us to find many new differentially expressed cytokines in published studies.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Won JH,Goldberger O,Shen-Orr SS,Davis MM,Olshen RA

doi

10.1073/pnas.1112599109

subject

Has Abstract

pub_date

2012-02-21 00:00:00

pages

2848-53

issue

8

eissn

0027-8424

issn

1091-6490

pii

1112599109

journal_volume

109

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Insufficiently dehydrated hydrogen bonds as determinants of protein interactions.

    abstract：:The prediction of binding sites and the understanding of interfaces associated with protein complexation remains an open problem in molecular biophysics. This work shows that a crucial factor in predicting and rationalizing protein-protein interfaces can be inferred by assessing the extent of intramolecular desolvatio...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0136888100

    authors： Fernández A,Scheraga HA

    更新日期：2003-01-07 00:00:00

  • Glucocorticoids locally disrupt an array of positioned nucleosomes on the rat tyrosine aminotransferase promoter in hepatoma cells.

    abstract：:Transcriptional activation by steroid hormones is often associated with the appearance of a DNase I hypersensitive site resulting from a local alteration of the nucleoprotein structure of the promoter. For the mouse mammary tumor virus long terminal repeat, a viral promoter under glucocorticoid control, a model has be...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.23.9300

    authors： Carr KD,Richard-Foy H

    更新日期：1990-12-01 00:00:00

  • The active site structure of Na+/K+-transporting ATPase: location of the 5'-(p-fluorosulfonyl)benzoyladenosine binding site and soluble peptides released by trypsin.

    abstract：:When the dog kidney Na+/K+-transporting ATPase (EC 3.6.1.37, formerly EC 3.6.1.3) was labeled with an ATP analogue, 5'-(p-fluorosulfonyl)benzoyladenosine (FSBA), there was a concomitant loss of ATPase activity. The presence of ATP protected the enzyme from both labeling and inactivation. The ATP-sensitive incorporatio...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.7.2071

    authors： Ohta T,Nagano K,Yoshida M

    更新日期：1986-04-01 00:00:00

  • Biliproteins of cyanobacteria and Rhodophyta: Homologous family of photosynthetic accessory pigments.

    abstract：:Amino-terminal sequence determinations are reported of the subunits of biliproteins of prokaryotic unicellular and filamentous cyanobacteria and of eukaryotic unicellular red algae. The biliproteins examined, allophycocyanin, C-phycocyanin, R-phycocyanin, b-phycoerythrin, and phycoerythrocyanin, vary with respect to t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.73.2.428

    authors： Glazer AN,Apell GS,Hixson CS,Bryant DA,Rimon S,Brown DM

    更新日期：1976-02-01 00:00:00

  • Evidence for circularization of the avian oncornavirus RNA genome during proviral DNA synthesis from studies of reverse transcription in vitro.

    abstract：:The RNA-directed DNA polymerase (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase EC 2.7.7.7) of avian oncornavirus requires a tryptophan tRNA (tRNATrp) primer molecule located close to the 5' end of the viral RNA genome for the initiation of DNA synthesis in vitro. In this communication we demonstrate that...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.73.4.1329

    authors： Collett MS,Faras AJ

    更新日期：1976-04-01 00:00:00

  • Activated Cdc42/Rac reconstitutes Fcepsilon RI-mediated Ca2+ mobilization and degranulation in mutant RBL mast cells.

    abstract：:Antigen stimulation of mast cells via FcepsilonRI, the high-affinity receptor for IgE, triggers a signaling cascade that requires Ca(2+) mobilization for exocytosis of secretory granules during an allergic response. This study investigates critical signaling components by using mutant RBL mast cells that are defective...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.98.3.1154

    authors： Hong-Geller E,Holowka D,Siraganian RP,Baird B,Cerione RA

    更新日期：2001-01-30 00:00:00

  • Nuclear repartitioning of galectin-1 by an extracellular glycan switch regulates mammary morphogenesis.

    abstract：:Branching morphogenesis in the mammary gland is achieved by the migration of epithelial cells through a microenvironment consisting of stromal cells and extracellular matrix (ECM). Here we show that galectin-1 (Gal-1), an endogenous lectin that recognizes glycans bearing N-acetyllactosamine (LacNAc) epitopes, induces ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1609135113

    authors： Bhat R,Belardi B,Mori H,Kuo P,Tam A,Hines WC,Le QT,Bertozzi CR,Bissell MJ

    更新日期：2016-08-16 00:00:00

  • Compact steep-spectrum sources and the unified scheme.

    abstract：:The compact steep-spectrum sources (CSSs) are an interesting class of objects which are of subgalactic dimensions; they occur more frequently in high-frequency surveys because their spectra often turn over at lower frequencies. We have estimated the symmetry parameters of a well-defined sample of CSSs and compared the...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.25.11417

    authors： Saikia DJ

    更新日期：1995-12-05 00:00:00

  • MAPK-directed activation of the whitefly transcription factor CREB leads to P450-mediated imidacloprid resistance.

    abstract：:The evolution of insect resistance to pesticides poses a continuing threat to agriculture and human health. While much is known about the proximate molecular and biochemical mechanisms that confer resistance, far less is known about the regulation of the specific genes/gene families involved, particularly by trans-act...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1913603117

    authors： Yang X,Deng S,Wei X,Yang J,Zhao Q,Yin C,Du T,Guo Z,Xia J,Yang Z,Xie W,Wang S,Wu Q,Yang F,Zhou X,Nauen R,Bass C,Zhang Y

    更新日期：2020-05-12 00:00:00

  • Cell-type-specific receptors for alpha-fetoprotein in a mouse T-lymphoma cell line.

    abstract：:Binding and uptake of alpha-fetoprotein (AFP) by mouse T-lymphoma YAC-1 cells exhibited the characteristics of receptor-mediated endocytosis. The binding saturation curve obtained by incubating YAC-1 cells at 4 degrees C with 125I-labeled AFP at different concentrations (50 ng/ml to 2.5 mg/ml) showed three saturation ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.10.3301

    authors： Naval J,Villacampa MJ,Goguel AF,Uriel J

    更新日期：1985-05-01 00:00:00

  • Imaging of a glioma using peripheral benzodiazepine receptor ligands.

    abstract：:Two types of benzodiazepine receptors have been demonstrated in mammalian tissues, one which is localized on neuronal elements in brain and the other, on glial cells and in peripheral tissues such as kidney. In vivo administration of 3H-labeled PK 11195 [1-(2-chlorophenyl-N-methyl-N-(1-methylpropyl)-3-isoquinoline car...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.3.891

    authors： Starosta-Rubinstein S,Ciliax BJ,Penney JB,McKeever P,Young AB

    更新日期：1987-02-01 00:00:00

  • Antarctic birds breed later in response to climate change.

    abstract：:In the northern hemisphere, there is compelling evidence for climate-related advances of spring events, but no such long-term biological time series exist for the southern hemisphere. We have studied a unique data set of dates of first arrival and laying of first eggs over a 55-year period for the entire community of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0510397103

    authors： Barbraud C,Weimerskirch H

    更新日期：2006-04-18 00:00:00

  • Structural analysis of a carcinogen-induced genomic rearrangement event.

    abstract：:We have explored the mechanism of genomic rearrangement in a hamster fibroblast cell culture system in which rearrangements are induced 5' to the endogenous thymidine kinase gene by chemical carcinogen treatment. The wild-type region around one rearrangement breakpoint was cloned and sequenced. With this sequence info...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.3.942

    authors： Barr FG,Davis RJ,Eichenfield L,Emanuel BS

    更新日期：1992-02-01 00:00:00

  • Time-evolving genetic networks reveal a NAC troika that negatively regulates leaf senescence in Arabidopsis.

    abstract：:Senescence is controlled by time-evolving networks that describe the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks for NAM/ATAF/CUC (NAC) transcription factors in Arabidopsis during leaf aging. The most evident characteristic of these time-dependent networks w...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1721523115

    authors： Kim HJ,Park JH,Kim J,Kim JJ,Hong S,Kim J,Kim JH,Woo HR,Hyeon C,Lim PO,Nam HG,Hwang D

    更新日期：2018-05-22 00:00:00

  • RNA from an immediate early region of the type 1 herpes simplex virus genome is present in the trigeminal ganglia of latently infected mice.

    abstract：:Transcription of the type 1 herpes simplex virus (HSV-1) genome in trigeminal ganglia of latently infected mice was studied using in situ hybridization. Probes representative of each temporal gene class were used to determine the regions of the genome that encode the transcripts present in latently infected cells. Pro...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.10.3204

    authors： Deatly AM,Spivack JG,Lavi E,Fraser NW

    更新日期：1987-05-01 00:00:00

  • Modulation of T-lymphocyte differentiation antigens: potential relevance for multiple sclerosis.

    abstract：:Effects of the anti-T-cell monoclonal antibodies OKT3, OKT5, and OKT8 on T-cell surface properties and cell functions were evaluated. Incubation of mononuclear cells isolated from peripheral blood for 48 hr with each monoclonal antibody in the absence of complement resulted in modulation of their respective surface an...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.10.3330

    authors： Antel J,Oger JJ,Jackevicius S,Kuo HH,Arnason BG

    更新日期：1982-05-01 00:00:00

  • Localization of Niemann-Pick C1 protein in astrocytes: implications for neuronal degeneration in Niemann- Pick type C disease.

    abstract：:Niemann-Pick type C disease (NP-C) is an inherited neurovisceral lipid storage disorder characterized by progressive neurodegeneration. Most cases of NP-C result from inactivating mutations of NPC1, a recently identified member of a family of genes encoding membrane-bound proteins containing putative sterol sensing do...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.4.1657

    authors： Patel SC,Suresh S,Kumar U,Hu CY,Cooney A,Blanchette-Mackie EJ,Neufeld EB,Patel RC,Brady RO,Patel YC,Pentchev PG,Ong WY

    更新日期：1999-02-16 00:00:00

  • A shared cis-regulatory module activates transcription in the suspensor of plant embryos.

    abstract：:The mechanisms controlling the transcription of gene sets in specific regions of a plant embryo shortly after fertilization remain unknown. Previously, we showed that G564 mRNA, encoding a protein of unknown function, accumulates to high levels in the giant suspensor of both Scarlet Runner Bean (SRB) and Common Bean e...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1805802115

    authors： Henry KF,Bui AQ,Kawashima T,Goldberg RB

    更新日期：2018-06-19 00:00:00

  • Developmentally regulated mannose 6-phosphate receptor-mediated transport of a lysosomal enzyme across the blood-brain barrier.

    abstract：:Mucopolysaccharidosis type VII is a lysosomal storage disorder resulting from inherited deficiency of beta-glucuronidase (GUS). Mucopolysaccharidosis type VII is characterized by glycosaminoglycan storage in most tissues, including brain. In these disorders, enzyme delivery across the blood-brain barrier (BBB) is the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0405042101

    authors： Urayama A,Grubb JH,Sly WS,Banks WA

    更新日期：2004-08-24 00:00:00

  • Inactivation of CREBBP expands the germinal center B cell compartment, down-regulates MHCII expression and promotes DLBCL growth.

    abstract：:The genes encoding the histone acetyl-transferases (HATs) CREB binding protein (CREBBP) and EP300 are recurrently mutated in the activated B cell-like and germinal center (GC) B cell-like subtypes of diffuse large B cell lymphoma (DLBCL). Here, we introduced a patient mutation into a human DLBCL cell line using CRISPR...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1619555114

    authors： Hashwah H,Schmid CA,Kasser S,Bertram K,Stelling A,Manz MG,Müller A

    更新日期：2017-09-05 00:00:00

  • Metachromatic leukodystrophy: arylsulfatase-A deficiency in skin fibroblast cultures.

    abstract：:Fibroblasts cultured from the skin of a patient with metachromatic leukodystrophy have been found to manifest the biochemical defect of this inborn error of metabolism, a deficiency of arylsulfatase A. Diseased cells had less than five per cent of normal arylsulfatase-A activity, while activities of other lysosomal en...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.62.3.887

    authors： Porter MT,Fluharty AL,Kihara H

    更新日期：1969-03-01 00:00:00

  • The rise of COVID-19 cases is associated with support for world leaders.

    abstract：:COVID-19 has emerged as one of the deadliest and most disruptive events in recent human history. Drawing from political science and psychological theories, we examine the effects of daily confirmed cases in a country on citizens' support for the political leader through the first 120 d of 2020. Using three unique data...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2009252117

    authors： Yam KC,Jackson JC,Barnes CM,Lau J,Qin X,Lee HY

    更新日期：2020-10-13 00:00:00

  • Cell water dynamics on multiple time scales.

    abstract：:Water-biomolecule interactions have been extensively studied in dilute solutions, crystals, and rehydrated powders, but none of these model systems may capture the behavior of water in the highly organized intracellular milieu. Because of the experimental difficulty of selectively probing the structure and dynamics of...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0709585105

    authors： Persson E,Halle B

    更新日期：2008-04-29 00:00:00

  • On the molecular mechanisms of transposition.

    abstract：:We present a model for transposition that allows a choice between cointegrate formation (replicon fusion) and direct transposition. We propose that initiation of the process occurs by invasion of the target DNA by a single-stranded end of the transposable element. This leads to nicking of one of the DNA strands of the...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.8.4858

    authors： Galas DJ,Chandler M

    更新日期：1981-08-01 00:00:00

  • Polyhedra and packings from hyperbolic honeycombs.

    abstract：:We derive more than 80 embeddings of 2D hyperbolic honeycombs in Euclidean 3 space, forming 3-periodic infinite polyhedra with cubic symmetry. All embeddings are "minimally frustrated," formed by removing just enough isometries of the (regular, but unphysical) 2D hyperbolic honeycombs [Formula: see text], [Formula: se...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1720307115

    authors： Pedersen MC,Hyde ST

    更新日期：2018-07-03 00:00:00

  • Topological impact of noncanonical DNA structures on Klenow fragment of DNA polymerase.

    abstract：:Noncanonical DNA structures that stall DNA replication can cause errors in genomic DNA. Here, we investigated how the noncanonical structures formed by sequences in genes associated with a number of diseases impacted DNA polymerization by the Klenow fragment of DNA polymerase. Replication of a DNA sequence forming an ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1704258114

    authors： Takahashi S,Brazier JA,Sugimoto N

    更新日期：2017-09-05 00:00:00

  • Primarily chronic progressive and relapsing/remitting multiple sclerosis: two immunogenetically distinct disease entities.

    abstract：:HLA class II gene polymorphism was investigated in 100 patients with clinically definite multiple sclerosis (MS) by restriction fragment length polymorphism analysis of Taq I-digested DNA using DRB, DQA, and DQB cDNA probes. Twenty-six patients had primarily chronic progressive MS and 74 had relapsing/remitting MS. Th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.18.7113

    authors： Olerup O,Hillert J,Fredrikson S,Olsson T,Kam-Hansen S,Möller E,Carlsson B,Wallin J

    更新日期：1989-09-01 00:00:00

  • Alpha-lactalbumin affects the acceptor specificity of Lymnaea stagnalis albumen gland UDP-GalNAc:GlcNAc beta-R beta 1-->4-N-acetylgalactosaminyltransferase: synthesis of GalNAc beta 1-->4Glc.

    abstract：:The N,N'-diacetyllactosediamine (lacdiNAc) pathway of complex-type oligosaccharide synthesis is controlled by a UDP-GalNAc:GlcNAc beta-R beta 1-->4-N-acetylgalac-tesaminyltransferase (beta 4-GalNAcT) that acts analogously to the common UDP-Gal:GlcNAc beta-R beta 1-->4-galactosyltransferase (beta 4-GalT). LacdiNAc-base...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.19.10111

    authors： Neeleman AP,van de Eijnden DH

    更新日期：1996-09-17 00:00:00

  • CYCLOPS reveals human transcriptional rhythms in health and disease.

    abstract：:Circadian rhythms modulate many aspects of physiology. Knowledge of the molecular basis of these rhythms has exploded in the last 20 years. However, most of these data are from model organisms, and translation to clinical practice has been limited. Here, we present an approach to identify molecular rhythms in humans f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1619320114

    authors： Anafi RC,Francey LJ,Hogenesch JB,Kim J

    更新日期：2017-05-16 00:00:00

  • Parental divorce is not uniformly disruptive to children's educational attainment.

    abstract：:Children whose parents divorce tend to have worse educational outcomes than children whose parents stay married. However, not all children respond identically to their parents divorcing. We focus on how the impact of parental divorce on children's education varies by how likely or unlikely divorce was for those parent...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1813049116

    authors： Brand JE,Moore R,Song X,Xie Y

    更新日期：2019-04-09 00:00:00