Immunocytochemical localization of prosomatostatin fragments in maturing and mature secretory granules of pancreatic and gastrointestinal D cells.

abstract：:Xenopus oocytes express a gene encoding bovine rhodopsin as well as its SP6 RNA polymerase-derived transcripts and total retinal mRNA. The opsin produced is in unglycosylated (30 kDa) and two glycosylated (35 kDa and 41 kDa) forms. Incubation of the cells expressing the above proteins with 11-cis-retinal generates rho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khorana HG,Knox BE,Nasi E,Swanson R,Thompson DA

更新日期：1988-11-01 00:00:00

abstract：:Information concerning the dynamics of river meandering is embedded in their planforms. Here, we focus on how bend skewing varies with increasing sinuosity, and how flow direction is embedded in bend skewing. It has often been thought that upstream-skewed bends are dominant within a sufficiently long reach. These bend...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo X,Chen D,Parker G

更新日期：2019-11-19 00:00:00

abstract：:G protein-coupled receptors (GPCRs) are seven-transmembrane proteins that transmit diverse extracellular signals across a membrane. Herpesvirus genomes encode multiple GPCRs implicated in viral pathogenesis. Kaposi sarcoma-associated herpesvirus GPCR (kGPCR) activates proliferative pathways and, when expressed in endo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Lu X,Zhu L,Shen Y,Chengedza S,Feng H,Wang L,Jung JU,Gutkind JS,Feng P

更新日期：2013-07-02 00:00:00

abstract：:Some realistic galaxy models have been found to support discrete unstable spiral modes. Here, through the study of the relevant physical mechanisms and an extensive numerical investigation of the properties of the dominant modes in a wide class of galactic equilibria, we show how spiral structures are excited with dif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haass J,Bertin G,Lin CC

更新日期：1982-06-01 00:00:00

abstract：:High-pressure methods are increasingly used to produce new dense materials with unusual properties. Increasing efforts to understand the reaction mechanisms at the microscopic level, to set up and optimize synthetic approaches, are currently directed at carbon-based solids. A fundamental, but still unsolved, question ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Citroni M,Bini R,Foggi P,Schettino V

更新日期：2008-06-03 00:00:00

abstract：:The difficulty of achieving robust functional expression of insect nicotinic acetylcholine receptors (nAChRs) has hampered our understanding of these important molecular targets of globally deployed neonicotinoid insecticides at a time when concerns have grown regarding the toxicity of this chemotype to insect pollina...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ihara M,Furutani S,Shigetou S,Shimada S,Niki K,Komori Y,Kamiya M,Koizumi W,Magara L,Hikida M,Noguchi A,Okuhara D,Yoshinari Y,Kondo S,Tanimoto H,Niwa R,Sattelle DB,Matsuda K

更新日期：2020-07-14 00:00:00

abstract：:As chips further shrink toward smaller scales, fabrication processes based on the self-assembly of individual particles into patterns or structures are often sought. One of the most popular techniques for two-dimensional assembly (self-assembled monolayers) is based on capillary forces acting on particles placed at a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aubry N,Singh P,Janjua M,Nudurupati S

更新日期：2008-03-11 00:00:00

abstract：:Peroxisome proliferators (PP) induce a highly predictable pleiotropic response in rat and mouse liver that is characterized by hepatomegaly, increase in peroxisome number in hepatocytes, and induction of certain peroxisomal enzymes. The PP-binding protein (PPbP) was purified from rat liver cytosol by a two-step proced...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lalwani ND,Alvares K,Reddy MK,Reddy MN,Parikh I,Reddy JK

更新日期：1987-08-01 00:00:00

abstract：:Competition for mates can be a major source of selection, not just on secondary sexual traits but across the genome. Mate competition strengthens selection on males via sexual selection, which typically favors healthy, vigorous individuals and, thus, all genetic variants that increase overall quality. However, recent ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yun L,Chen PJ,Kwok KE,Angell CS,Rundle HD,Agrawal AF

更新日期：2018-06-26 00:00:00

abstract：:The 26S proteasome, a molecular machine responsible for regulated protein degradation, consists of a proteolytic core particle (20S CP) associated with 19S regulatory particles (19S RPs) subdivided into base and lid subcomplexes. The assembly of 19S RP base subcomplex is mediated by multiple dedicated chaperones. Amon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barrault MB,Richet N,Godard C,Murciano B,Le Tallec B,Rousseau E,Legrand P,Charbonnier JB,Le Du MH,Guérois R,Ochsenbein F,Peyroche A

更新日期：2012-04-24 00:00:00

abstract：:Adults and older children are more likely to punish a wrongdoer for a moral transgression when the victim belongs to their group. Building on these results, in violation-of-expectation experiments (n = 198), we examined whether 2.5-year-old toddlers (Exps. 1 and 2) and 1-year-old infants (Exps. 3 and 4) would selectiv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ting F,He Z,Baillargeon R

更新日期：2019-03-26 00:00:00

abstract：:Human declarative memory involves a systematic organization of information that supports generalizations and inferences from acquired knowledge. This kind of memory depends on the hippocampal region in humans, but the extent to which animals also have declarative memory, and whether inferential expression of memory de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dusek JA,Eichenbaum H

更新日期：1997-06-24 00:00:00

abstract：:In Saccharomyces cerevisiae cells harboring a GAL1 promoter-linked beta-galactosidase gene, the simultaneous expression of Escherichia coli DNA topoisomerase I and inactivation of yeast DNA topoisomerases I and II reduces the cellular level of beta-galactosidase to an undetectable level. Analysis of intracellular mRNA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gartenberg MR,Wang JC

更新日期：1992-12-01 00:00:00

abstract：:Swarming and mass migration are spectacular and sometimes devastating features of the biology of various animal species. These phenomena are typically associated with actual or anticipated depletion of food resources after an increase in population density, but the mechanisms driving such collective movements are poor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simpson SJ,Sword GA,Lorch PD,Couzin ID

更新日期：2006-03-14 00:00:00

abstract：:The rate of derepressed synthesis of a membrane protein required for lactose transport (M protein) by Escherichia coli is increased in response to increased gene dosage to the same extent as the rates of synthesis of beta-galactosidase and galactoside acetylase. However, elevated gene dosage does not increase beta-gal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fox CF

更新日期：1969-07-01 00:00:00

abstract：:Previous work has established stargazin and its related family of transmembrane AMPA receptor regulatory proteins (TARPs) as auxiliary subunits of AMPA receptors (AMPARs) that control synaptic strength both by targeting AMPARs to synapses through an intracellular PDZ-binding motif and by modulating their gating throug...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Milstein AD,Nicoll RA

更新日期：2009-07-07 00:00:00

abstract：:Signaling in the nervous system requires matching of neurotransmitter receptors with cognate neurotransmitters at synapses. The vertebrate neuromuscular junction is the best studied cholinergic synapse, but the mechanisms by which acetylcholine is matched with acetylcholine receptors are not fully understood. Because ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Borodinsky LN,Spitzer NC

更新日期：2007-01-02 00:00:00

abstract：:The regulation of protein function through ligand-induced conformational changes is crucial for many signal transduction processes. The binding of a ligand alters the delicate energy balance within the protein structure, eventually leading to such conformational changes. In this study, we elucidate the energetic and m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bauer D,Merz DR,Pelz B,Theisen KE,Yacyshyn G,Mokranjac D,Dima RI,Rief M,Žoldák G

更新日期：2015-08-18 00:00:00

abstract：:A new nondefective hybrid virus has been plaque-isolated from the Ad.2-SV40 hybrid population. This virus replicates efficiently with one-hit kinetics in both human embryonic kidney and African green monkey kidney cells, induces an SV40 specific antigen which is detectable by immunofluorescence and complement-fixation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lewis AM Jr,Levin MJ,Wiese WH,Crumpacker CS,Henry PH

更新日期：1969-08-01 00:00:00

abstract：:Transport of sodium, chloride, and taurocholate was studied in primary cultures of adult rat hepatocytes incubated in a balanced electrolyte solution containing 150 mM NaCl, various concentrations of taurocholate, and (22)Na, (36)Cl, [(3)H]taurocholate, and 3-O-[(3)H]methyl-D-glucose. Lithium chloride, choline chlorid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scharschmidt BF,Stephens JE

更新日期：1981-02-01 00:00:00

abstract：:Recent studies on proteins whose N and C termini are in close proximity have demonstrated that folding of polypeptide chains and assembly of oligomers can be accomplished with circularly permuted chains. As yet no methodical study has been conducted to determine how extensively new termini can be introduced and where ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Graf R,Schachman HK

更新日期：1996-10-15 00:00:00

abstract：:Insects, as a group, have been remarkably successful in adapting to a great range of physical and biological environments, in large part because of their ability to fly. The evolution of flight in small insects was accompanied by striking adaptations of the thoracic musculature that enabled very high wing beat frequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swank DM,Vishnudas VK,Maughan DW

更新日期：2006-11-14 00:00:00

abstract：:The molecular mechanism of tolerance to opiate drugs is poorly understood. We have used single-channel patch-clamp recordings to study opiate receptor effects on dissociated neurons from rat amygdala, a limbic region implicated in addiction processes. A 130-pS inwardly rectifying K(+)-preferring cation channel was act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Marrero HG,Murphy R,Lin YJ,Freedman JE

更新日期：2000-12-19 00:00:00

abstract：:Operating rooms (ORs) are inhabited by hierarchical, mixed-gender clinical teams that are often prone to conflict. In evolutionary terms, one expects more within- than between-gender rivalries, especially since the OR is a place where all sorts of social interactions occur, not merely technical communications. To docu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jones LK,Jennings BM,Higgins MK,de Waal FBM

更新日期：2018-07-17 00:00:00

abstract：:Complex social behavior in Microtus voles and other mammals has been postulated to be under the direct genetic control of a single locus: the arginine vasopressin 1a receptor (avpr1a) gene. Using a phylogenetic approach, we show that a repetitive element in the promoter region of avpr1a, which reportedly causes social...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fink S,Excoffier L,Heckel G

更新日期：2006-07-18 00:00:00

abstract：:The brain contains an astonishing diversity of neurons, each expressing only one set of ion channels out of the billions of potential channel combinations. Simple organizing principles are required for us to make sense of this abundance of possibilities and wealth of related data. We suggest that energy minimization s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hasenstaub A,Otte S,Callaway E,Sejnowski TJ

更新日期：2010-07-06 00:00:00

abstract：:The assembly and composition of human excision nuclease were investigated by electrophoretic mobility shift assay and DNase I footprinting. Individual repair factors or any combination of up to four repair factors failed to form DNA-protein complexes of high specificity and stability. A stable complex of high specific...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wakasugi M,Sancar A

更新日期：1998-06-09 00:00:00

abstract：:A cyclophilin (CyP) purified to homogeneity from the polycentric anaerobic rumen fungus Orpinomyces sp. strain PC-2 had a molecular mass of 20.5 kDa and a pI of 8.1. The protein catalyzed the isomerization of the prolyl peptide bond of N-succinyl-Ala-Ala-(cis,trans)-Pro-Phe p-nitroanilide with a kcat/Km value of 9.3 x...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen H,Li XL,Ljungdahl LG

更新日期：1995-03-28 00:00:00

abstract：:Comparative genomics of closely related bacterial isolates is a powerful method for uncovering virulence and other important genome elements. We determined draft sequences (8-fold coverage) of the genomes of strains JD1 and N40 of Borrelia burgdorferi sensu stricto, the causative agent of Lyme disease, and we compared...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qiu WG,Schutzer SE,Bruno JF,Attie O,Xu Y,Dunn JJ,Fraser CM,Casjens SR,Luft BJ

更新日期：2004-09-28 00:00:00

abstract：:Clostridium kluyveri grown in the presence of 1 muM Na2(75)SeO3 produces a thiolase that copurifies with 75Se. Based on several criteria, the selenium moiety in this protein is selenomethionine. The 75Se-labeled amino acid in acid hydrolysates of the radioactive protein cochromatographed with authentic selenomethionin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hartmanis MG,Stadtman TC

更新日期：1982-08-01 00:00:00