Isolation of a selenium-containing thiolase from Clostridium kluyveri: identification of the selenium moiety as selenomethionine.

abstract：:Residential location is thought to influence people's well-being, but different individuals may value residential areas differently. We examined how life satisfaction and personality traits are geographically distributed within the UK London metropolitan area, and how the strength of associations between personality t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jokela M,Bleidorn W,Lamb ME,Gosling SD,Rentfrow PJ

更新日期：2015-01-20 00:00:00

abstract：:DNA methylation is an epigenetic mark that silences transposable elements (TEs) and repeats. Whereas the establishment and maintenance of DNA methylation are relatively well understood, little is known about their dynamics and biological relevance in plant and animal innate immunity. Here, we show that some TEs are de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu A,Lepère G,Jay F,Wang J,Bapaume L,Wang Y,Abraham AL,Penterman J,Fischer RL,Voinnet O,Navarro L

更新日期：2013-02-05 00:00:00

abstract：:Antibiotic resistance in bacteria has emerged as a global challenge over the past 90 years, compromising our ability to effectively treat infections. There has been a dramatic increase in antibiotic resistance-associated determinants in bacterial populations, driven by the mobility and infectious nature of such determ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klemm EJ,Wong VK,Dougan G

更新日期：2018-12-18 00:00:00

abstract：:The cAMP receptor protein (CRP) of Escherichia coli needs cAMP for an allosteric change to regulate gene expression by binding to specific DNA sites. The hinge region connecting the DNA-binding domain to the cAMP-binding domain has been proposed to participate in the cAMP-induced allosteric change necessary to adjust ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ryu S,Kim J,Adhya S,Garges S

更新日期：1993-01-01 00:00:00

abstract：:Both treatment of disease and basic studies of complex tissues can benefit from directing viral vector infection to specific cell types. We have used a unique cell targeting method to direct viral vector transduction to cerebral cortical neurons expressing the neuregulin (NRG) receptor ErbB4; both NRG and ErbB4 have b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Choi J,Young JA,Callaway EM

更新日期：2010-09-21 00:00:00

abstract：:GM54VA human cells transformed by simian virus 40 (SV40) were fused with peritoneal macrophages obtained from three different mouse strains. All 27 hybrid clones studied were positive for SV40 tumor antigen in 100% of their cells and contained human chromosome 17. Human chromosome 17 was the only human chromosome pres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Croce CM

更新日期：1977-01-01 00:00:00

abstract：:Class Ib Qa-2 molecules are expressed in tissue culture cells as approximately 40-kDa membrane-bound, glycophosphatidylinositol-linked antigens and as approximately 39-kDa soluble polypeptides. Recently, alternative splicing events which delete exon 5 from a portion of Qa-2 transcripts were demonstrated to give rise t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tabaczewski P,Shirwan H,Lewis K,Stroynowski I

更新日期：1994-03-01 00:00:00

abstract：:The c-ras family is a set of c-onc genes that are highly conserved in vertebrates. The genes in this family are homologous to the transforming genes of Harvey and Kirsten murine sarcoma viruses (v-Ha-ras and v-Ki-ras, respectively). Using an in situ molecular hybridization method, we detected three sites on the human ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jhanwar SC,Neel BG,Hayward WS,Chaganti RS

更新日期：1983-08-01 00:00:00

abstract：:Two genes have a synthetically lethal relationship when the silencing or inhibiting of 1 gene is only lethal in the context of a mutation or activation of the second gene. This situation offers an attractive therapeutic strategy, as inhibition of such a gene will only trigger cell death in tumor cells with an activate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Molenaar JJ,Ebus ME,Geerts D,Koster J,Lamers F,Valentijn LJ,Westerhout EM,Versteeg R,Caron HN

更新日期：2009-08-04 00:00:00

abstract：:The conversion of light energy into ion gradients across biological membranes is one of the most fundamental reactions in primary biological energy transduction. Recently, the structure of the first light-activated Na+ pump, Krokinobacter eikastus rhodopsin 2 (KR2), was resolved at atomic resolution [Kato HE, et al. (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suomivuori CM,Gamiz-Hernandez AP,Sundholm D,Kaila VRI

更新日期：2017-07-03 00:00:00

abstract：:A 24-amino acid residue peptide has been isolated from the Fc region of a human IgG1 myeloma protein. The peptide has associated with it the same ability to induce murine B cells to polyclonally secrete antibody as does the intact Fc fragment. Amino acid composition of the peptide indicates that on a mole/mole basis t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morgan EL,Hugli TE,Weigle WO

更新日期：1982-09-01 00:00:00

abstract：:To elucidate cellular diversity and clonal evolution in tissues and tumors, one must resolve genomic heterogeneity in single cells. To this end, we have developed low-cost, mass-producible micro-/nanofluidic chips for DNA extraction from individual cells. These chips have modules that collect genomic DNA for sequencin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marie R,Pedersen JN,Bærlocher L,Koprowska K,Pødenphant M,Sabatel C,Zalkovskij M,Mironov A,Bilenberg B,Ashley N,Flyvbjerg H,Bodmer WF,Kristensen A,Mir KU

更新日期：2018-10-30 00:00:00

abstract：:Despite the increasing number of regulatory proteins identified in clathrin-independent endocytic (CIE) pathways, our understanding of the exact functions of these proteins and the sequential manner in which they function remains limited. In this study, using the Caenorhabditis elegans intestine as a model, we observe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen S,Li L,Li J,Liu B,Zhu X,Zheng L,Zhang R,Xu T

更新日期：2014-10-28 00:00:00

abstract：:We evaluated the formation and removal of (+-)-3 alpha,4 beta-dihydroxy-1 alpha,2 alpha-epoxy-1,2,3,4- tetrahydrobenzo[c]phenanthrene (BcPHDE)-DNA adducts in two Chinese hamster ovary (CHO) cell lines. One line of repair-proficient cells (MK42) carries a stable 150-fold amplification of the dihydrofolate reductase (DH...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carothers AM,Zhen W,Mucha J,Zhang YJ,Santella RM,Grunberger D,Bohr VA

更新日期：1992-12-15 00:00:00

abstract：:Acanthamoeba castellanii contains two enzymes, myosins IA and IB, that exhibit the catalytic properties of a myosin but possess very unusual physical properties, the most striking of which are their single, low molecular weight heavy chain, their globular shape, and their inability to form bipolar filaments. We have n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hammer JA 3rd,Jung G,Korn ED

更新日期：1986-07-01 00:00:00

abstract：:The interference of microtubular disruptors with the uptake of amino acids and other low molecular weight substrates has been studied in Morris hepatomas, host liver, and regenerating liver. Colchicine inhibits amino acid transport (alpha-aminoisobutyric acid, L-methionine, and L-leucine) in hepatomas by 59-98% wherea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tauber R,Reutter W

更新日期：1980-09-01 00:00:00

abstract：:Portable, single-sided NMR sensors can operate under conditions inaccessible to conventional NMR while featuring lower cost, portability, and the ability to analyze arbitrary-sized objects. Such sensors can nondestructively probe the interior of samples by collecting images and measuring relaxation and diffusion const...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paulsen JL,Bouchard LS,Graziani D,Blümich B,Pines A

更新日期：2008-12-30 00:00:00

abstract：:Activation of the syk tyrosine kinase occurs almost immediately following engagement of many types of antigen receptors, including Fc receptors, but the mechanism through which syk is activated is currently unclear. Here we demonstrate that Fc receptor-induced syk activation occurs as the result of phosphorylation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： El-Hillal O,Kurosaki T,Yamamura H,Kinet JP,Scharenberg AM

更新日期：1997-03-04 00:00:00

abstract：:Transcript accumulation and translation of psbA as well as processing of the D1 precursor protein were investigated in relation to chlorophyll availability in vivo in cyanobacterial strains lacking photosystem I (PS I). The psbA transcript level was almost independent of chlorophyll availability and was approximately ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He Q,Vermaas W

更新日期：1998-05-12 00:00:00

abstract：:Mutations in the human DYNC1H1 gene are associated with neurological diseases. DYNC1H1 encodes the heavy chain of cytoplasmic dynein-1, a 1.4-MDa motor complex that traffics organelles, vesicles, and macromolecules toward microtubule minus ends. The effects of the DYNC1H1 mutations on dynein motility, and consequently...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoang HT,Schlager MA,Carter AP,Bullock SL

更新日期：2017-02-28 00:00:00

abstract：:A mechanism of ion transport across membranes is reported. Microbial transport of Fe(3+) generally delivers iron, a growth-limiting nutrient, to cells via highly specific siderophore-mediated transport systems. In contrast, iron transport in the fresh water bacterium Aeromonas hydrophila is found to occur by means of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stintzi A,Barnes C,Xu J,Raymond KN

更新日期：2000-09-26 00:00:00

abstract：:Sleep is essential for basic survival, and insufficient sleep leads to a variety of dysfunctions. In humans, one of the most profound consequences of sleep deprivation is imprecise or irrational communication, demonstrated by degradation in signaling as well as in receiving information. Communication in nonhuman anima...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klein BA,Klein A,Wray MK,Mueller UG,Seeley TD

更新日期：2010-12-28 00:00:00

abstract：:Particular major histocompatibility complex (MHC) class II alleles clearly contribute to T cell-mediated autoimmune type 1 diabetes (T1D) in both humans and nonobese diabetic (NOD) mice. However, studies in NOD mice indicate MHC class I-restricted T cell responses are also essential to T1D development. In humans, epid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marron MP,Graser RT,Chapman HD,Serreze DV

更新日期：2002-10-15 00:00:00

abstract：:When expressed in Escherichia coli, the human beta 1- and beta 2-adrenergic receptors retain their ligand binding specificity. Their functional integrity was investigated by analyzing receptor-guanine nucleotide-binding regulatory (G) protein coupling by using two splice variants of the alpha subunit of the stimulator...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Freissmuth M,Selzer E,Marullo S,Schütz W,Strosberg AD

更新日期：1991-10-01 00:00:00

abstract：:d-alanine-d-lactate ligase from Enterococcus faecium BM4147 is directly responsible for the biosynthesis of alternate cell-wall precursors in bacteria, which are resistant to the glycopeptide antibiotic vancomycin. The crystal structure has been determined with data extending to 2.5-A resolution. This structure shows ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roper DI,Huyton T,Vagin A,Dodson G

更新日期：2000-08-01 00:00:00

abstract：:Ubiquitylation of proteins can be a signal for a variety of cellular processes beyond the classical role in proteolysis. The different signaling functions of ubiquitylation are thought to rely on ubiquitin-binding domains (UBDs). Several distinct UBD families are known, but their functions are not understood in detail...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tyrrell A,Flick K,Kleiger G,Zhang H,Deshaies RJ,Kaiser P

更新日期：2010-11-16 00:00:00

abstract：:The results of previous preclinical and clinical studies have identified angiogenin (ANG) as a potentially important target for anticancer therapy. Here we report the design and implementation of a high-throughput screening assay to identify small molecules that bind to the ribonucleolytic active site of ANG, which is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kao RY,Jenkins JL,Olson KA,Key ME,Fett JW,Shapiro R

更新日期：2002-07-23 00:00:00

abstract：:In epithelial cells, sorting of membrane proteins to the basolateral surface depends on the presence of a basolateral sorting signal (BaSS) in their cytoplasmic domain. Amyloid precursor protein (APP), a basolateral protein implicated in the pathogenesis of Alzheimer's disease, contains a tyrosine-based BaSS, and muta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zheng P,Eastman J,Vande Pol S,Pimplikar SW

更新日期：1998-12-08 00:00:00

abstract：:Contingency models of feeding compare the energy per unit time gained from utilizing a resource unit of a particular kind (food types, habitat patches, time periods) against that energy/time expected if the unit is skipped. Optimally, an animal should reject the particular unit if and only if the former energy/time is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schoener TW

更新日期：1974-10-01 00:00:00

abstract：:Simulations with a regional chemical transport model show that anthropogenic emissions of volatile organic compounds and nitrogen oxides (NO(x) = NO + NO(2)) lead to a dramatic diurnal variation of surface ozone (O(3)) in Houston, Texas. During the daytime, photochemical oxidation of volatile organic compounds catalyz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang R,Lei W,Tie X,Hess P

更新日期：2004-04-27 00:00:00