Positive regulation of minichromosome maintenance gene expression, DNA replication, and cell transformation by a plant retinoblastoma gene.

abstract：:Depression is a debilitating mental illness and is often comorbid with metabolic disorders such as type 2 diabetes. Adiponectin is an adipocyte-derived hormone with antidiabetic and insulin-sensitizing properties. Here we show that adiponectin levels in plasma are reduced in a chronic social-defeat stress model of dep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu J,Guo M,Zhang D,Cheng SY,Liu M,Ding J,Scherer PE,Liu F,Lu XY

更新日期：2012-07-24 00:00:00

abstract：:Horizontal cells of the carp retina possess dopamine receptors linked to adenylate cyclase. Isolated, intact horizontal cells respond to micromolar concentrations of dopamine, whereas nanomolar concentrations of haloperidol, (+)-butaclamol, and flupenthixol block the dopamine response. Preincubation in Ringer's soluti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Buskirk R,Dowling JE

更新日期：1982-05-01 00:00:00

abstract：:Children whose parents divorce tend to have worse educational outcomes than children whose parents stay married. However, not all children respond identically to their parents divorcing. We focus on how the impact of parental divorce on children's education varies by how likely or unlikely divorce was for those parent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brand JE,Moore R,Song X,Xie Y

更新日期：2019-04-09 00:00:00

abstract：:Crithidia fasciculata cells were treated with a plasmid (pDK96) containing pBR322 sequences, a Leishmania tarentolae maxicircle autonomously replicating sequence, and the bacterial gene for aminoglycoside 3' phosphotransferase I inserted between the yeast alcohol dehydrogenase 1 promotor and terminator sequences. Resi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes DE,Simpson L

更新日期：1986-08-01 00:00:00

abstract：:Inadequate target exposure is a major cause of high attrition in drug discovery. Here, we show that a label-free method for quantifying the intracellular bioavailability (Fic) of drug molecules predicts drug access to intracellular targets and hence, pharmacological effect. We determined Fic in multiple cellular assay...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mateus A,Gordon LJ,Wayne GJ,Almqvist H,Axelsson H,Seashore-Ludlow B,Treyer A,Matsson P,Lundbäck T,West A,Hann MM,Artursson P

更新日期：2017-07-25 00:00:00

abstract：:Changes in protein function can lead to changes in the selection acting on specific residues. This can often be detected as evolutionary rate changes at the sites in question. A maximum-likelihood method for detecting evolutionary rate shifts at specific protein positions is presented. The method determines significan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knudsen B,Miyamoto MM

更新日期：2001-12-04 00:00:00

abstract：:Binding of carbon monoxide and oxygen to sterically protected heme model compounds (basket-handle porphyrins) was investigated in liquid toluene at temperatures from 180 to 300 K by laser flash photolysis. Only a single exponential rebinding process from the solvent could be seen in the time range of 20 nsec to millis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tetreau C,Lavalette D,Momenteau M,Lhoste JM

更新日期：1987-04-01 00:00:00

abstract：:The ability of beta cells to endure assaults may be relevant in the development of insulin-dependent diabetes mellitus. This study examines the susceptibility of human pancreatic islets to agents that are cytotoxic for rodent beta cells--i.e., sodium nitroprusside (NP, a nitric oxide donor), streptozotocin (SZ), or al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eizirik DL,Pipeleers DG,Ling Z,Welsh N,Hellerström C,Andersson A

更新日期：1994-09-27 00:00:00

abstract：:The present study investigated whether neural structures become less functionally differentiated and specialized with age. We studied ventral visual cortex, an area of the brain that responds selectively to visual categories (faces, places, and words) in young adults, and that shows little atrophy with age. Functional...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park DC,Polk TA,Park R,Minear M,Savage A,Smith MR

更新日期：2004-08-31 00:00:00

abstract：:Subunits A and B of chloroplast glyceraldehyde-3-phosphate dehydrogenase are synthesized as higher molecular weight precursors when polyadenylylated mRNA from angiosperm seedlings is translated in vitro by wheat germ ribosomes. The in vivo levels of mRNA coding for these precursors are strongly light dependent, and th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cerff R,Kloppstech K

更新日期：1982-12-01 00:00:00

abstract：:Neurofibrillary tangles are one of two lesions found in the brain of Alzheimer disease victims. With synthetic peptide fragments of human neurofilament NF-M17 (Glu-Glu-Lys-Gly-Lys-Ser-Pro- Val-Pro-Lys-Ser-Pro-Val-Glu-Glu-Lys-Gly, phosphorylated and unphosphorylated), CD studies were done to examine the effect of sodiu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fasman GD,Moore CD

更新日期：1994-11-08 00:00:00

abstract：:Visualizing macromolecular complexes by single-particle electron microscopy (EM) entails stringent biochemical purification, specimen preparation, low-dose imaging, and 3D image reconstruction. Here, we introduce the "monolayer purification" method, which employs nickel-nitrilotriacetic acid (Ni-NTA) functionalized li...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelly DF,Dukovski D,Walz T

更新日期：2008-03-25 00:00:00

abstract：:A three-plasmid system for heterologous expression of 6-deoxyerythronolide B synthase (DEBS) has been developed to facilitate combinatorial biosynthesis of polyketides made by type I modular polyketide synthases (PKSs). The eryA PKS genes encoding the three DEBS subunits were individually cloned into three compatible ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xue Q,Ashley G,Hutchinson CR,Santi DV

更新日期：1999-10-12 00:00:00

abstract：:Chloroplast DNA of the protozoan flagellate, Euglena gracilis, exists as circular molecules, 40 mum in contour length, as shown by electron microscopy and buoyant density analyses. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manning JE,Wolstenholme DR,Ryan RS,Hunter JA,Richards OC

更新日期：1971-06-01 00:00:00

abstract：:The biological roles of estrogen-responsive finger protein (efp) in vivo were evaluated in mice carrying a loss-of-function mutation in efp by gene-targeted mutagenesis. Although efp homozygous mice were viable and fertile in both sexes, the uterus that expressed abundant estrogen receptor alpha exhibited significant ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Orimo A,Inoue S,Minowa O,Tominaga N,Tomioka Y,Sato M,Kuno J,Hiroi H,Shimizu Y,Suzuki M,Noda T,Muramatsu M

更新日期：1999-10-12 00:00:00

abstract：:The exceptional sensitivity of Mycobacterium tuberculosis to isonicotinic acid hydrazide (INH) lacks satisfactory definition. M. tuberculosis is a natural mutant in oxyR, a central regulator of peroxide stress response. The ahpC gene, which encodes a critical subunit of alkyl hydroperoxide reductase, is one of the tar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Y,Dhandayuthapani S,Deretic V

更新日期：1996-11-12 00:00:00

abstract：:T cells that have been immunized to express optimal levels of contact hypersensitivity upon adoptive transfer to normal animals can be inhibited from doing so by incubating them with an antigen-specific T suppressor factor. This factor is composed of at least two subunits which come from cells expressing different Ly ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ptak W,Rosenstein RW,Gershon RK

更新日期：1982-04-01 00:00:00

abstract：:Electron spectroscopic imaging, a new technique that permits the quantitative detection of the spatial distributions of atomic elements at high resolution, has been applied to the epiphyseal zone of hypertrophy in the mouse for the visualization of calcium, phosphorus, and sulfur. Longitudinally sectioned epiphyseal g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arsenault AL,Ottensmeyer FP

更新日期：1983-03-01 00:00:00

abstract：:The anticonvulsants phenytoin and carbamazepine interact allosterically with the batrachotoxin binding site of sodium channels. In the present study, we demonstrate an autoradiographic technique to localize the batrachotoxin binding site on sodium channels in rat brain using [3H]batrachotoxinin-A 20-alpha-benzoate (BT...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Worley PF,Baraban JM

更新日期：1987-05-01 00:00:00

abstract：:Cigarette smoke is the major cause of lung cancer, the leading cause of cancer death, and of chronic obstructive pulmonary disease, the fourth leading cause of death in the United States. Using high-density gene expression arrays, we describe genes that are normally expressed in a subset of human airway epithelial cel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spira A,Beane J,Shah V,Liu G,Schembri F,Yang X,Palma J,Brody JS

更新日期：2004-07-06 00:00:00

abstract：:We establish size limitations for assembling structures of controlled size and shape out of colloidal particles with short-ranged interactions. Through simulations we show that structures with highly variable shapes made out of dozens of particles can form with high yield, as long as each particle in the structure bin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeravcic Z,Manoharan VN,Brenner MP

更新日期：2014-11-11 00:00:00

abstract：:Although it is known that the c-myc oncogene is rearranged in a head-to-head fashion with the immunoglobulin heavy chain locus in mouse plasmacytomas, it has not been clear whether the c-myc oncogene is translocated to the heavy chain locus on mouse chromosome 12 or whether the heavy chain locus is translocated to the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Erikson J,Miller DA,Miller OJ,Abcarian PW,Skurla RM,Mushinski JF,Croce CM

更新日期：1985-06-01 00:00:00

abstract：:The HIV type 1 (HIV-1) virion infectivity factor (Vif) protein blocks the action of the host defense factor APOBEC3G in human cells, thereby allowing release of infectious virions, but fails to inhibit similar APOBEC3G proteins present in some simian cells. Conversely, the Vif protein encoded by the African green monk...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bogerd HP,Doehle BP,Wiegand HL,Cullen BR

更新日期：2004-03-16 00:00:00

abstract：:Two independently arising alpha heavy chain mutants have been found to synthesize heavy chains with CH1 deletions of approximately equal extent. Both were isolated from heavy chain-producing variants of the mouse myeloma W3129 and demonstrate that it is possible to arrive at the heavy chain disease phenotype by the pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dackowski W,Morrison SL

更新日期：1981-11-01 00:00:00

abstract：:We have constructed a hybrid E beta gene by replacing the second exon of the Ed beta gene (which encodes the majority of the beta 1 protein domain) with the corresponding exon from the Eb beta gene. The hybrid gene has been introduced into a d haplotype host, the lymphoma beta-cell line A20-2J, and an E alpha E beta d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Folsom V,Gay D,Tonegawa S

更新日期：1985-03-01 00:00:00

abstract：:The up-regulation of the 25-hydroxyvitamin D(3)-24-hydroxylase by 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] is well established and occurs at the transcriptional level through two vitamin D response elements in the promoter of the gene. However, the mechanism of down-regulation of the 24-hydroxylase by parathyroid ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zierold C,Mings JA,DeLuca HF

更新日期：2001-11-20 00:00:00

abstract：:Studies were undertaken to determine the applicability and effectiveness of immunoadsorption chromatography on anti-low density lipoprotein (LDL) columns as a plasma-cholesterol-lowering procedure. Mass production and isolation of monospecific antibodies against swine LDL (rho = 1.006-1.063 g/ml) was carried out by im...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stoffel W,Demant T

更新日期：1981-01-01 00:00:00

abstract：:We have devised a method for mapping sites on DNA within the nucleus that are protected against nuclease attack by interaction with bound protein or other factors. This "footprinting" method uses an end-labeled sequence-specific DNA probe, which is annealed to the DNA from nuclear digests under carefully controlled co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jackson PD,Felsenfeld G

更新日期：1985-04-01 00:00:00

abstract：:Myelodysplastic syndromes (MDS) are among the most frequent hematologic malignancies. Patients have a short survival and often progress to acute myeloid leukemia. The diagnosis of MDS can be difficult; there is a paucity of molecular markers, and the pathophysiology is largely unknown. Therefore, we conducted a multic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aivado M,Spentzos D,Germing U,Alterovitz G,Meng XY,Grall F,Giagounidis AA,Klement G,Steidl U,Otu HH,Czibere A,Prall WC,Iking-Konert C,Shayne M,Ramoni MF,Gattermann N,Haas R,Mitsiades CS,Fung ET,Libermann TA

更新日期：2007-01-23 00:00:00

abstract：:This work describes the rational design, synthesis, and study of a ligand that selectively complexes CUG repeats in RNA (and CTG repeats in DNA) with high nanomolar affinity. This sequence is considered a causative agent of myotonic dystrophy type 1 (DM1) because of its ability to sequester muscleblind-like (MBNL) pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arambula JF,Ramisetty SR,Baranger AM,Zimmerman SC

更新日期：2009-09-22 00:00:00