Abstract:
:Inadequate target exposure is a major cause of high attrition in drug discovery. Here, we show that a label-free method for quantifying the intracellular bioavailability (Fic) of drug molecules predicts drug access to intracellular targets and hence, pharmacological effect. We determined Fic in multiple cellular assays and cell types representing different targets from a number of therapeutic areas, including cancer, inflammation, and dementia. Both cytosolic targets and targets localized in subcellular compartments were investigated. Fic gives insights on membrane-permeable compounds in terms of cellular potency and intracellular target engagement, compared with biochemical potency measurements alone. Knowledge of the amount of drug that is locally available to bind intracellular targets provides a powerful tool for compound selection in early drug discovery.
journal_name
Proc Natl Acad Sci U S Aauthors
Mateus A,Gordon LJ,Wayne GJ,Almqvist H,Axelsson H,Seashore-Ludlow B,Treyer A,Matsson P,Lundbäck T,West A,Hann MM,Artursson Pdoi
10.1073/pnas.1701848114subject
Has Abstractpub_date
2017-07-25 00:00:00pages
E6231-E6239issue
30eissn
0027-8424issn
1091-6490pii
1701848114journal_volume
114pub_type
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