Estrogen differentially regulates neuropeptide gene expression in a sexually dimorphic olfactory pathway.

abstract：:8-Azido[2-3H]adenine was used as a photoaffinity label for the purine-cytosine transport system. After irradiation in the presence of the photoaffinity label, the cells were converted into protoplasts, their plasma membranes were purified, and the membrane proteins were extracted and separated by NaDodSO4/PAGE. The ra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmidt R,Manolson MF,Chevallier MR

更新日期：1984-10-01 00:00:00

abstract：:Current evidence favors the theory that, when the globular motor domain of myosin attaches to actin, the light chain binding domain or "lever arm" rotates, and thereby generates movement of actin filaments. Myosin is uniquely designed for such a role in that a long alpha-helix (approximately 9 nm) extending from the C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sherwood JJ,Waller GS,Warshaw DM,Lowey S

更新日期：2004-07-27 00:00:00

abstract：:A water-soluble component of membranes of cells from a Burkitt's lymphoma cultured cell line was used to develop an antiserum that detects an antigen(s) on acute leukemia cells. This antiserum did not react with nonlymphoid cultured cell lines except for human embryonic kidney cells infected with the Rauscher murine l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mann DL,Halterman R,Leventhal BG

更新日期：1973-02-01 00:00:00

abstract：:Much experimental work has been devoted in comparing the folding behavior of proteins sharing the same fold but different sequence. The recent design of proteins displaying very high sequence identities but different 3D structure allows the unique opportunity to address the protein-folding problem from a complementary...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giri R,Morrone A,Travaglini-Allocatelli C,Jemth P,Brunori M,Gianni S

更新日期：2012-10-30 00:00:00

abstract：:OpcA is an integral outer membrane protein from Neisseria meningitidis, the causative agent of meningococcal meningitis and septicemia. It mediates the adhesion of N. meningitidis to epithelial and endothelial cells by binding to vitronectin and proteoglycan cell-surface receptors. Here, we report the determination of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prince SM,Achtman M,Derrick JP

更新日期：2002-03-19 00:00:00

abstract：:T cells interacting with antigen-presenting cells (APCs) form an "immunological synapse" (IS), a bull's-eye pattern composed of a central supramolecular activation cluster enriched with T cell receptors (TCRs) surrounded by a ring of adhesion molecules (a peripheral supramolecular activation cluster). The mechanism re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaizuka Y,Douglass AD,Varma R,Dustin ML,Vale RD

更新日期：2007-12-18 00:00:00

abstract：:Several disulfide benzamides have been shown to possess wide-spectrum antiretroviral activity in cell culture at low micromolar to submicromolar concentrations, inhibiting human immunodeficiency virus (HIV) type 1 (HIV-1) clinical and drug-resistant strains along with HIV-2 and simian immunodeficiency virus [Rice, W. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tummino PJ,Scholten JD,Harvey PJ,Holler TP,Maloney L,Gogliotti R,Domagala J,Hupe D

更新日期：1996-02-06 00:00:00

abstract：:The S(N)2 displacement of Cl(-) from 1,2-dichloroethane by acetate (CH(3)CO(2)(-)) in water and by the carboxylate of the active site aspartate in the haloalkane dehalogenase of Xanthobacter autothropicus have been compared by using molecular dynamics simulations. In aqueous solution, six families of contact-pair stru...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hur S,Kahn K,Bruice TC

更新日期：2003-03-04 00:00:00

abstract：:Positional cloning has already produced the sequences of more than 70 human genes associated with specific diseases. In addition to their medical importance, these genes are of interest as a set of human genes isolated solely on the basis of the phenotypic effect of the respective mutations. We analyzed the protein se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mushegian AR,Bassett DE Jr,Boguski MS,Bork P,Koonin EV

更新日期：1997-05-27 00:00:00

abstract：:The gene networks underlying closure of the optic fissure during vertebrate eye development are poorly understood. Here, we profile global gene expression during optic fissure closure using laser capture microdissected (LCM) tissue from the margins of the fissure. From these data, we identify a unique role for the C(2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown JD,Dutta S,Bharti K,Bonner RF,Munson PJ,Dawid IB,Akhtar AL,Onojafe IF,Alur RP,Gross JM,Hejtmancik JF,Jiao X,Chan WY,Brooks BP

更新日期：2009-02-03 00:00:00

abstract：:The N-methyl-d-aspartate (NMDA) glutamate receptor (NMDAR), long implicated in developmental plasticity, shows decay time kinetics that shorten postnatally as NR2A subunits are added to the receptor. Neither the mechanism nor immediate effect of this change is known. We studied developing NMDAR currents by using visua...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Townsend M,Yoshii A,Mishina M,Constantine-Paton M

更新日期：2003-02-04 00:00:00

abstract：:Phylogenetic differences in acetylcholine receptors from skeletal neuromuscular synapses of various species of snakes and lizards have been investigated, using the snake venom alpha-neurotoxins alpha-atratoxin (cobrotoxin) and alpha-bungarotoxin. The acetylcholine receptors of the phylogenetically primitive lizards, l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burden SJ,Hartzell HC,Yoshikami D

更新日期：1975-08-01 00:00:00

abstract：:Several next-generation (universal) influenza vaccines and broadly neutralizing antibodies (bNAbs) are in clinical development. Some of these mediate inhibitions of virus replication at the postentry stage or use Fc-dependent mechanisms. Nonneutralizing antibodies have the potential to mediate enhancement of viral inf...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Winarski KL,Tang J,Klenow L,Lee J,Coyle EM,Manischewitz J,Turner HL,Takeda K,Ward AB,Golding H,Khurana S

更新日期：2019-07-23 00:00:00

abstract：:Burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease in the tropics and subtropics with high morbidity and mortality. The facultative intracellular bacterium induces host cell fusion through its type VI secretion system 5 (T6SS5) as an important part of its pathogenesis in mammalian h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ku JWK,Chen Y,Lim BJW,Gasser S,Crasta KC,Gan YH

更新日期：2020-07-07 00:00:00

abstract：:The active conformation of native peroxisomal 3-ketoacyl-CoA thiolases (EC 2.3.1.16) is homodimeric. We have previously shown that a truncated Saccharomyces cerevisiae thiolase lacking its first 16 N-terminal amino acids fails to be translocated into peroxisomes but assembles into an enzymatically active form in the c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glover JR,Andrews DW,Rachubinski RA

更新日期：1994-10-25 00:00:00

abstract：:In most human follicular B cell lymphomas the bcl-2 gene is up-regulated as a result of the t(14;18) chromosomal translocation generating a hybrid bcl-2-IgH mRNA. Recently, we have identified in t(14;18)-positive cells a bcl-2-IgH mRNA in the antisense orientation, putatively responsible for the overexpression of bcl-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morelli S,Delia D,Capaccioli S,Quattrone A,Schiavone N,Bevilacqua A,Tomasini S,Nicolin A

更新日期：1997-07-22 00:00:00

abstract：:The tips of the digits of some mammals, including human infants and mice, are capable of complete regeneration after injury. This process is reliant on the presence of the overlaying nail organ and is mediated by a proliferative blastema. Epithelial Wnt/β-catenin signaling has been shown to be necessary for mouse digi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehoczky JA,Tabin CJ

更新日期：2015-10-27 00:00:00

abstract：:In this paper, we introduce a method to account for the shape of the potential energy curve in the evaluation of conformational free energies. The method is based on a procedure that generates a set of conformations, each with its own force-field energy, but adds a term to this energy that favors conformations that ar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiang Z,Soto CS,Honig B

更新日期：2002-05-28 00:00:00

abstract：:The molecular basis for the absence of anthocyanins and proanthocyanidins in four independent sodium azide-induced ant18 mutants of barley was examined by sequencing the gene encoding dihydroflavonol 4-reductase in these mutants. Sodium azide generated 21 base substitutions, which corresponds to 0.17% of the 12,704 nu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olsen O,Wang X,von Wettstein D

更新日期：1993-09-01 00:00:00

abstract：:An intense investigation of possible non-Fermi liquid states of matter has been inspired by two of the most intriguing phenomena discovered in the past quarter century, namely, high-temperature superconductivity and the fractional quantum Hall effect. Despite enormous conceptual strides, these two fields have develope...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jain JK,Anderson PW

更新日期：2009-06-09 00:00:00

abstract：:The key enzyme for transcription of protein-encoding genes in eukaryotes is RNA polymerase II (RNAPII). The recruitment of this enzyme during transcription initiation and its passage along the template during transcription elongation is regulated through the association and dissociation of several complexes. Elongator...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nelissen H,Fleury D,Bruno L,Robles P,De Veylder L,Traas J,Micol JL,Van Montagu M,Inzé D,Van Lijsebettens M

更新日期：2005-05-24 00:00:00

abstract：:The Na,K-ATPase contains a binding site for cardiac glycosides, such as ouabain, digoxin, and digitoxin, which is highly conserved among species ranging from Drosophila to humans. Although advantage has been taken of this site to treat congestive heart failure with drugs such as digoxin, it is unknown whether this sit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dostanic-Larson I,Van Huysse JW,Lorenz JN,Lingrel JB

更新日期：2005-11-01 00:00:00

abstract：:Signal transducer and activator of transcription 3 (STAT3) is a multifunctional protein that participates in signaling pathways initiated by various growth factors and cytokines. It exists in multiple forms including those phosphorylated on Tyr(705) (pYSTAT3) or Ser(727) (pSSTAT3) as well as the unphosphorylated prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koo MY,Park J,Lim JM,Joo SY,Shin SP,Shim HB,Chung J,Kang D,Woo HA,Rhee SG

更新日期：2014-04-29 00:00:00

abstract：:Toward understanding their redundancies and interactions in hemostasis and thrombosis, we examined the roles of thrombin receptors (protease-activated receptors, PARs) and the ADP receptor P2RY12 (purinergic receptor P2Y G protein-coupled 12) in human and mouse platelets ex vivo and in mouse models. Par3(-/-) and Par4...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cornelissen I,Palmer D,David T,Wilsbacher L,Concengco C,Conley P,Pandey A,Coughlin SR

更新日期：2010-10-26 00:00:00

abstract：:Unconventional myosin 15 is a molecular motor expressed in inner ear hair cells that transports protein cargos within developing mechanosensory stereocilia. Mutations of myosin 15 cause profound hearing loss in humans and mice; however, the properties of this motor and its regulation within the stereocilia organelle a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bird JE,Takagi Y,Billington N,Strub MP,Sellers JR,Friedman TB

更新日期：2014-08-26 00:00:00

abstract：:To study C(0)a priori estimates for solutions to certain complex Monge-Ampère equations, I introduce a coherent sheaf of ideals and show that it satisfies various global algebrogeometric conditions, including a cohomology vanishing theorem. This technique is used to establish the existence of Kähler-Einstein metrics o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nadel AM

更新日期：1989-10-01 00:00:00

abstract：:Mouse teratocarcinoma cells induced to differentiate in vitro undergo a massive (30%) demethylation of DNA. A similar undermethylation is also observed in the mouse extraembryonic membranes, the yolk sac and placenta. In both cases, the decrease in methyl moieties occurs at a large number of CpG sites spread out over ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Razin A,Webb C,Szyf M,Yisraeli J,Rosenthal A,Naveh-Many T,Sciaky-Gallili N,Cedar H

更新日期：1984-04-01 00:00:00

abstract：:Virus-specific or group-specific antibody probes to potyviruses can be produced by targeting the immune response to the virus-specific, N-terminal region of the capsid protein (29-95 amino acids depending on the virus) or to the conserved core region (216 amino acids) of the capsid protein, respectively. Immunochemica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shukla DD,Tribbick G,Mason TJ,Hewish DR,Geysen HM,Ward CW

更新日期：1989-11-01 00:00:00

abstract：:We have established a transformation system for Dictyostelium discoideum. The transformation vector contains the protein coding region of the Tn5 neomycin resistance gene fused to the proposed promoter of the Dictyostelium actin 8 gene; the vector also contains a sequence that acts as an autonomously replicating seque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirth KP,Edwards CA,Firtel RA

更新日期：1982-12-01 00:00:00

abstract：:Neonates are highly susceptible to infectious diseases and, in general, respond poorly to conventional vaccines due to immaturity of the immune system. In the present study, we hypothesized that the anti-tetanus toxoid (TT) vaccine response of neonatal mice could be enhanced by retinoic acid (RA), a bioactive retinoid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma Y,Ross AC

更新日期：2005-09-20 00:00:00