Abstract:
:The gene networks underlying closure of the optic fissure during vertebrate eye development are poorly understood. Here, we profile global gene expression during optic fissure closure using laser capture microdissected (LCM) tissue from the margins of the fissure. From these data, we identify a unique role for the C(2)H(2) zinc finger proteins Nlz1 and Nlz2 in normal fissure closure. Gene knockdown of nlz1 and/or nlz2 in zebrafish leads to a failure of the optic fissure to close, a phenotype which closely resembles that seen in human uveal coloboma. We also identify misregulation of pax2 in the developing eye of morphant fish, suggesting that Nlz1 and Nlz2 act upstream of the Pax2 pathway in directing proper closure of the optic fissure.
journal_name
Proc Natl Acad Sci U S Aauthors
Brown JD,Dutta S,Bharti K,Bonner RF,Munson PJ,Dawid IB,Akhtar AL,Onojafe IF,Alur RP,Gross JM,Hejtmancik JF,Jiao X,Chan WY,Brooks BPdoi
10.1073/pnas.0812017106subject
Has Abstractpub_date
2009-02-03 00:00:00pages
1462-7issue
5eissn
0027-8424issn
1091-6490pii
0812017106journal_volume
106pub_type
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