Relation between native ensembles and experimental structures of proteins.

abstract：:The plant kingdom contains vastly untapped natural product chemistry, which has been traditionally explored through the activity-guided approach. Here, we describe a gene-guided approach to discover and engineer a class of plant ribosomal peptides, the branched cyclic lyciumins. Initially isolated from the Chinese wol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kersten RD,Weng JK

更新日期：2018-11-13 00:00:00

abstract：:Diphosphoinositol pentakisphosphate (PP-IP5) and bis(diphospho)inositol tetrakisphosphate (bis-PP-IP4) are recently identified inositol phosphates that possess pyrophosphate bonds. We have purified an inositol hexakisphosphate (IP6) kinase from rat brain supernatants. The pure protein, a monomer of 54 kDa, displays hi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Voglmaier SM,Bembenek ME,Kaplin AI,Dormán G,Olszewski JD,Prestwich GD,Snyder SH

更新日期：1996-04-30 00:00:00

abstract：:The binding of killer cell Ig-like Receptors (KIR) to their Class I MHC ligands was shown previously to be characterized by extremely rapid association and dissociation rate constants. During experiments to investigate the biochemistry of receptor-ligand binding in more detail, the kinetic parameters of the interactio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Valés-Gómez M,Erskine RA,Deacon MP,Strominger JL,Reyburn HT

更新日期：2001-02-13 00:00:00

abstract：:We argue that folding of the compact domains of proteins can occur with adequate rapidity in the absence of a unique directed mechanism, provided that native-like local structure dominates the folding process. We further suggest that the evolution of amino acid sequences should favor multiple paths to the folded state...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harrison SC,Durbin R

更新日期：1985-06-01 00:00:00

abstract：:Metabolic, infectious, and tumor cell-intrinsic noxae can all evoke the endoplasmic reticulum (ER) stress response in tumor cells, which is critical for tumor cell growth and cancer progression. Evidence exists that the ER stress response can drive a proinflammatory program in tumor cells and macrophages but, to our k...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mahadevan NR,Rodvold J,Sepulveda H,Rossi S,Drew AF,Zanetti M

更新日期：2011-04-19 00:00:00

abstract：:cDNA clones for the (S)-tetrahydroberberine (H4Ber) oxidase of cultured berberine-producing Coptis japonica cells were isolated by screening a C. japonica cDNA library with synthetic nucleotides that can encode the NH2-terminal sequence of this enzyme. Analyses of the nucleotide sequences of the cloned cDNA inserts re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okada N,Koizumi N,Tanaka T,Ohkubo H,Nakanishi S,Yamada Y

更新日期：1989-01-01 00:00:00

abstract：:Pollen tubes are highly polarized plant cells specialized in delivering sperm for fertilization. Pollen tube growth is rapid, occurs exclusively at the tip, and can reach distances thousands of times the diameter of the pollen grain without cell division, thus representing an excellent model system for studying asymme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheung AY,Niroomand S,Zou Y,Wu HM

更新日期：2010-09-14 00:00:00

abstract：:Inherited deficiencies of the enzymes adenosine deaminase (adenosine aminohydrolase; EC 3.5.4.4) and purine nucleoside phosphorylase (purine-nucleoside:orthophosphate ribosyltransferase; EC 2.4.2.1) preferentially interfere with lymphocyte development while sparing most other organ systems. Previous experiments have s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carson DA,Kaye J,Seegmiller JE

更新日期：1977-12-01 00:00:00

abstract：:What are the neural bases of semantic memory? Traditional beliefs that the temporal lobes subserve the retrieval of semantic knowledge, arising from lesion studies, have been recently called into question by functional neuroimaging studies finding correlations between semantic retrieval and activity in left prefrontal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thompson-Schill SL,Swick D,Farah MJ,D'Esposito M,Kan IP,Knight RT

更新日期：1998-12-22 00:00:00

abstract：:Integrins contain two structurally homologous but distantly related domains: an I-like domain that is present in all beta-subunits and an I domain that is present in some alpha-subunits. Atomic resolution and mutagenesis studies of alpha I domains demonstrate a C-terminal, axial displacement of the alpha7-helix that a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang W,Shimaoka M,Chen J,Springer TA

更新日期：2004-02-24 00:00:00

abstract：:The development of Drosophila requires the function of the CREB-binding protein, dCBP. In flies, dCBP serves as a coactivator for the transcription factors Cubitus interruptus, Dorsal, and Mad, and as a cosuppressor of Drosophila T cell factor. Current models propose that CBP, through its intrinsic and associated hist...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bantignies F,Goodman RH,Smolik SM

更新日期：2002-03-05 00:00:00

abstract：:A cloned complementary DNA sequence has been isolated from a human placental cDNA library in the bacteriophage expression vector lambda gt11 after screening with polyclonal antibodies against human placental aromatase-system cytochrome P-450 (P-450Arom). A single recombinant clone, lambda hAROM1, was characterized by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Evans CT,Ledesma DB,Schulz TZ,Simpson ER,Mendelson CR

更新日期：1986-09-01 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) viral DNA synthesis in quiescent and activated peripheral blood lymphocytes (PBLs) was studied. Incomplete viral DNA (previously demonstrated to be associated with HIV-1 virions) is carried by HIV-1 virions into quiescent and activated PBLs, contributing to the formation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao WY,Cara A,Gallo RC,Lori F

更新日期：1993-10-01 00:00:00

abstract：:In this paper we demonstrate that near infrared Fourier transform Raman spectroscopy provides unprecedented biochemical information about the extent of atherosclerosis in human aorta. In particular, elastin, collagen, cholesterol, cholesterol esters, lipids, carotenoids, and calcium apatite deposits all can be discern...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baraga JJ,Feld MS,Rava RP

更新日期：1992-04-15 00:00:00

abstract：:Previous work showed that cultured human pancreatic cancer cells overexpress the epidermal growth factor (EGF) receptor. In the present study, we sought to determine whether some of these cell lines produce transforming growth factor alpha (TGF-alpha). Utilizing a radiolabeled TGF-alpha cDNA in hybridization experimen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith JJ,Derynck R,Korc M

更新日期：1987-11-01 00:00:00

abstract：:Phage phi 29 DNA cannot be phosphorylated with polynucleotide kinase and [gamma-32P]ATP because of the presence of a viral protein covalently linked to the 5' termini. The 5' ends can, however, be made susceptible to phosphorylation by treatment with alkali and alkaline phosphatase. Restriction fragments Hpa II C and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Escarmís C,Salas M

更新日期：1981-03-01 00:00:00

abstract：:Event-related brain potentials (ERPs) to colored slides contained a late positive component that was significantly enhanced when adults recognized the person, place, or painting in the photograph. Additionally, two late components change in amplitude, corresponding to the amount of surprise reported. Because subjects ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neville H,Snyder E,Woods D,Galambos R

更新日期：1982-03-01 00:00:00

abstract：:The Toll/IL-1 receptor (TIR) domains are crucial signaling modules during innate immune responses involving the Toll-like receptors (TLRs) and IL-1 receptor (IL-1R). Myeloid differential factor 88 (MyD88) is a central TIR domain-containing adapter molecule responsible for nearly all TLR-mediated signaling and is targe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Snyder GA,Cirl C,Jiang J,Chen K,Waldhuber A,Smith P,Römmler F,Snyder N,Fresquez T,Dürr S,Tjandra N,Miethke T,Xiao TS

更新日期：2013-04-23 00:00:00

abstract：:Twice each year, millions of night-migratory songbirds migrate thousands of kilometers. To find their way, they must process and integrate spatiotemporal information from a variety of cues including the Earth's magnetic field and the night-time starry sky. By using sensory-driven gene expression, we discovered that ni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mouritsen H,Feenders G,Liedvogel M,Wada K,Jarvis ED

更新日期：2005-06-07 00:00:00

abstract：:Tentoxin, a natural cyclic tetrapeptide produced by phytopathogenic fungi from the Alternaria species affects the catalytic function of the chloroplast F(1)-ATPase in certain sensitive species of plants. In this study, we show that the uncompetitive inhibitor tentoxin binds to the alphabeta-interface of the chloroplas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Groth G

更新日期：2002-03-19 00:00:00

abstract：:The activation of additional promoter sites by production of an alternative sigma subunit for RNA polymerase is a common strategy for the coordinate regulation of gene expression. Many alternative sigma factors control genes for specialized, and often narrowly distributed, functions. For example, most of the alternati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen YF,Helmann JD

更新日期：1992-06-01 00:00:00

abstract：:Glial cells are traditionally regarded as elements for structural support and ionic homeostasis, but have recently attracted attention as putative integral elements of the machinery involved in synaptic transmission and plasticity. Here, we demonstrate that calcium-binding protein S100B, which is synthesized in consid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishiyama H,Knopfel T,Endo S,Itohara S

更新日期：2002-03-19 00:00:00

abstract：:In the usual Darwinian case in which struggle for existence leads to density limitations on the environment's carrying capacity, R. A. Fisher's reproductive-value concept reduces to zero for every initial age group. To salvage some meaning for Fisher's notion, two variant reproductive-value concepts are defined here: ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Samuelson PA

更新日期：1978-12-01 00:00:00

abstract：:We have analyzed the level of intraindividual sequence variability (heteroplasmy) of mtDNA in human brain by denaturing gradient gel electrophoresis and sequencing. Single base substitutions, as well as insertions or deletions of single bases, were numerous in the noncoding control region (D-loop), and 35-45% of the m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jazin EE,Cavelier L,Eriksson I,Oreland L,Gyllensten U

更新日期：1996-10-29 00:00:00

abstract：:We have evaluated two synthetic epothilone analogues lacking the 12,13-epoxide functionality, 12,13-desoxyepothilone B (dEpoB), and 12,13-desoxyepothilone F (dEpoF). The concentrations required for 50% growth inhibition (IC(50)) for a variety of anticancer agents were measured in CCRF-CEM/VBL1000 cells (2,048-fold res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chou TC,O'Connor OA,Tong WP,Guan Y,Zhang ZG,Stachel SJ,Lee C,Danishefsky SJ

更新日期：2001-07-03 00:00:00

abstract：:H-NS and Lsr2 are nucleoid-associated proteins from Gram-negative bacteria and Mycobacteria, respectively, that play an important role in the silencing of horizontally acquired foreign DNA that is more AT-rich than the resident genome. Despite the fact that Lsr2 and H-NS proteins are dissimilar in sequence and structu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon BR,Li Y,Cote A,Weirauch MT,Ding P,Hughes TR,Navarre WW,Xia B,Liu J

更新日期：2011-06-28 00:00:00

abstract：:Palmitoylation is a common lipid modification known to regulate the functional properties of various proteins and is a vital step in the biosynthesis of voltage-activated sodium (Nav) channels. We discovered a mutation in an intracellular loop of rNav1.2a (G1079C), which results in a higher apparent affinity for exter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bosmans F,Milescu M,Swartz KJ

更新日期：2011-12-13 00:00:00

abstract：:Rat serine dehydratase cDNA clones were isolated from a lambda gt11 cDNA library on the basis of their reactivity with monospecific immunoglobulin to the purified enzyme. Using the cDNA insert from a clone that encoded the serine dehydratase subunit as a probe, additional clones were isolated from the same library by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ogawa H,Miller DA,Dunn T,Su Y,Burcham JM,Peraino C,Fujioka M,Babcock K,Pitot HC

更新日期：1988-08-01 00:00:00

abstract：:Early abstract reasoning has typically been characterized by a "relational shift," in which children initially focus on object features but increasingly come to interpret similarity in terms of structured relations. An alternative possibility is that this shift reflects a learned bias, rather than a typical waypoint a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carstensen A,Zhang J,Heyman GD,Fu G,Lee K,Walker CM

更新日期：2019-07-09 00:00:00

abstract：:A tandemly repeated DNA sequence organized predominantly, if not entirely, in a specific manner on the human X chromosome has been cloned in pBR322 and characterized. The sequence was detected as a 2-kilobase band in ethidium bromide-stained agarose gels of BamHI-digested total human nuclear DNA. Although in situ hybr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang TP,Hansen SK,Oishi KK,Ryder OA,Hamkalo BA

更新日期：1982-11-01 00:00:00