Abstract:
:Lufenuron is an insect growth regulator insecticide mainly used for the control of the cat flea. To understand mechanisms of resistance to lufenuron, we have characterized lufenuron resistance in a natural population of Drosophila melanogaster. In this study we have used precise genetic mapping to identify a mechanism of lufenuron resistance: the overexpression of the cytochrome P450 gene Cyp12a4. Cyp12a4 is predicted to encode a mitochondrial cytochrome P450 enzyme. Expression of Cyp12a4 in D. melanogaster third-instar larvae was detected in the midgut and Malpighian tubules of both lufenuron-resistant and wild-type strains. The level of Cyp12a4 expression in the midgut is higher in the lufenuron-resistant strain than in wild-type strains. Driving the expression of Cyp12a4 in the midgut and Malpighian tubules by using the GAL4/UAS gene expression system results in lufenuron resistance, but it does not result in resistance to three other insecticide classes. Transgenic expression of Cyp12a4 in a ubiquitous expression pattern results in late embryonic lethality, suggesting that high-level ectopic expression of Cyp12a4 is detrimental to development.
journal_name
Proc Natl Acad Sci U S Aauthors
Bogwitz MR,Chung H,Magoc L,Rigby S,Wong W,O'Keefe M,McKenzie JA,Batterham P,Daborn PJdoi
10.1073/pnas.0503709102keywords:
subject
Has Abstractpub_date
2005-09-06 00:00:00pages
12807-12issue
36eissn
0027-8424issn
1091-6490pii
0503709102journal_volume
102pub_type
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