当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis.

Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis.

Abstract：

:Conventional asymmetric PCR is inefficient and difficult to optimize because limiting the concentration of one primer lowers its melting temperature below the reaction annealing temperature. Linear-After-The-Exponential (LATE)-PCR describes a new paradigm for primer design that renders assays as efficient as symmetric PCR assays, regardless of primer ratio. LATE-PCR generates single-stranded products with predictable kinetics for many cycles beyond the exponential phase. LATE-PCR also introduces new probe design criteria that uncouple hybridization probe detection from primer annealing and extension, increase probe reliability, improve allele discrimination, and increase signal strength by 80-250% relative to symmetric PCR. These improvements in PCR are particularly useful for real-time quantitative analysis of target numbers in small samples. LATE-PCR is adaptable to high throughput applications in fields such as clinical diagnostics, biodefense, forensics, and DNA sequencing. We showcase LATE-PCR via amplification of the cystic fibrosis CFDelta508 allele and the Tay-Sachs disease TSD 1278 allele from single heterozygous cells.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Sanchez JA,Pierce KE,Rice JE,Wangh LJ

doi

10.1073/pnas.0305476101

keywords:

subject

Has Abstract

pub_date

2004-02-17 00:00:00

pages

1933-8

issue

7

eissn

0027-8424

issn

1091-6490

pii

0305476101

journal_volume

101

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Neutron-capture therapy of human cancer: in vivo results on tumor localization of boron-10-labeled antibodies to carcinoembryonic antigen in the GW-39 tumor model system.

    abstract：:Antibody against carcinoembryonic antigen (CEA) was conjugated with p-[1,2-dicarba-closo-[1-3H]do-decaboran(12)-2-yl] benzenediazonium ion by an azo-coupling reaction, resulting in 30 boron atoms per IgG molecule with no loss of antibody protein. Antibody immunoreactivity was not appreciably affected by this conjugati...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.2.560

    authors： Goldenberg DM,Sharkey RM,Primus FJ,Mizusawa E,Hawthorne MF

    更新日期：1984-01-01 00:00:00

  • Destabilization of Ca2+-free gelsolin may not be responsible for proteolysis in Familial Amyloidosis of Finnish Type.

    abstract：:Mutations at position 187 in secreted gelsolin enable aberrant proteolysis at the 172-173 and 243-244 amide bonds, affording the 71-residue amyloidogenic peptide deposited in Familial Amyloidosis of Finnish Type (FAF). Thermodynamic comparisons of two different domain 2 constructs were carried out to study possible ef...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.041452598

    authors： Ratnaswamy G,Huff ME,Su AI,Rion S,Kelly JW

    更新日期：2001-02-27 00:00:00

  • Reducing Jagged 1 and 2 levels prevents cerebral arteriovenous malformations in matrix Gla protein deficiency.

    abstract：:Cerebral arteriovenous malformations (AVMs) are common vascular malformations, which may result in hemorrhagic strokes and neurological deficits. Bone morphogenetic protein (BMP) and Notch signaling are both involved in the development of cerebral AVMs, but the cross-talk between the two signaling pathways is poorly u...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1310905110

    authors： Yao Y,Yao J,Radparvar M,Blazquez-Medela AM,Guihard PJ,Jumabay M,Boström KI

    更新日期：2013-11-19 00:00:00

  • Computation of large molecules with the hartree-fock model.

    abstract：:The usual way to compute Hartree-Fock type functions for molecules is by an expansion of the one-electron functions (molecular orbitals) in a linear combination of analytical functions (LCAO-MO-SCF, linear combination of atomic orbitals-Molecular Orbital-Self Consistent field). The expansion coefficients are obtained ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.69.10.2942

    authors： Clementi E

    更新日期：1972-10-01 00:00:00

  • Selective blockers of voltage-gated K+ channels depolarize human T lymphocytes: mechanism of the antiproliferative effect of charybdotoxin.

    abstract：:Charybdotoxin (ChTX), a K+ channel blocker, depolarizes human peripheral T lymphocytes and renders them insensitive to activation by mitogen. We observed four types of K+ channels in human T cells: one voltage-activated, and three Ca(2+)-activated. To discern the mechanism by which ChTX depolarizes T cells, we examine...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.21.10094

    authors： Leonard RJ,Garcia ML,Slaughter RS,Reuben JP

    更新日期：1992-11-01 00:00:00

  • Feasibility of long-term storage of graded information by the Ca2+/calmodulin-dependent protein kinase molecules of the postsynaptic density.

    abstract：:The feasibility of long-term information storage by brain type II Ca2+/calmodulin-dependent protein kinase molecules is explored. Recent evidence indicates that this protein has switch-like properties. Equations are derived showing that a single kinase holoenzyme could form a bistable switch having the stability neces...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.14.5320

    authors： Lisman JE,Goldring MA

    更新日期：1988-07-01 00:00:00

  • Increased interleukin 12 production in progressive multiple sclerosis: induction by activated CD4+ T cells via CD40 ligand.

    abstract：:Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system postulated to be a cell-mediated autoimmune disease in which interferon gamma (IFN-gamma) plays an important role. There is increased IFN-gamma secretion in MS, and IFN-gamma administration induces exacerbations of disease. We foun...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.2.599

    authors： Balashov KE,Smith DR,Khoury SJ,Hafler DA,Weiner HL

    更新日期：1997-01-21 00:00:00

  • Targeted mutagenesis of Lis1 disrupts cortical development and LIS1 homodimerization.

    abstract：:Lissencephaly is a severe brain malformation in humans. To study the function of the gene mutated in lissencephaly (LIS1), we deleted the first coding exon from the mouse Lis1 gene. The deletion resulted in a shorter protein (sLIS1) that initiates from the second methionine, a unique situation because most LIS1 mutati...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.101122598

    authors： Cahana A,Escamez T,Nowakowski RS,Hayes NL,Giacobini M,von Holst A,Shmueli O,Sapir T,McConnell SK,Wurst W,Martinez S,Reiner O

    更新日期：2001-05-22 00:00:00

  • Convergent evolution toward an improved growth rate and a reduced resistance range in Prochlorococcus strains resistant to phage.

    abstract：:Prochlorococcus is an abundant marine cyanobacterium that grows rapidly in the environment and contributes significantly to global primary production. This cyanobacterium coexists with many cyanophages in the oceans, likely aided by resistance to numerous co-occurring phages. Spontaneous resistance occurs frequently i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1420347112

    authors： Avrani S,Lindell D

    更新日期：2015-04-28 00:00:00

  • Rous sarcoma virus activates embryonic globin genes in chicken fibroblasts.

    abstract：:Complementary DNA (cDNA) specific for chick globin mRNA sequences fails to hybridize to total RNA extracted from chicken fibroblasts. After infection by Rous sarcoma virus, RNA complementary to globin cDNA is detectable in 100-500 copies per cell. Infection of fibroblasts with the transformation defective (td) deletio...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.11.4464

    authors： Groudine M,Weintraub H

    更新日期：1975-11-01 00:00:00

  • Unified structure theory of icosahedral quasicrystals: Evidence from neutron powder diffraction patterns that AlCrFeMnSi, AlCuLiMg, and TiNiFeSi icosahedral quasicrystals are twins of cubic crystals containing about 820 or 1012 atoms in a primitive unit c

    abstract：:A unified structure theory of icosahedral quasicrystals, combining the twinned-cubic-crystal theory and the Penrose-tiling-six-dimensional-projection theory, is described. Values of the primitive-cubic lattice constant for several quasicrystals are evaluated from x-ray and neutron diffraction data. The fact that the l...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.22.8376

    authors： Pauling L

    更新日期：1988-11-01 00:00:00

  • Intimate bacterial-fungal interaction triggers biosynthesis of archetypal polyketides in Aspergillus nidulans.

    abstract：:Fungi produce numerous low molecular weight molecules endowed with a multitude of biological activities. However, mining the full-genome sequences of fungi indicates that their potential to produce secondary metabolites is greatly underestimated. Because most of the biosynthesis gene clusters are silent under laborato...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0901870106

    authors： Schroeckh V,Scherlach K,Nützmann HW,Shelest E,Schmidt-Heck W,Schuemann J,Martin K,Hertweck C,Brakhage AA

    更新日期：2009-08-25 00:00:00

  • Supervillin associates with androgen receptor and modulates its transcriptional activity.

    abstract：:Activation of androgen receptor (AR) via androgen in muscle cells has been closely linked to their growth and differentiation. Here, we report the cloning and characterization of supervillin (SV), a 205-kDa actin-binding protein, as an AR coregulator from the skeletal muscle cDNA library. Mammalian two-hybrid and glut...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.022469899

    authors： Ting HJ,Yeh S,Nishimura K,Chang C

    更新日期：2002-01-22 00:00:00

  • Expression of the REB transcriptional activator in rice grains improves the yield of recombinant proteins whose genes are controlled by a Reb-responsive promoter.

    abstract：:The gene encoding the rice transcription factor, REB (rice endosperm bZIP) was cloned from a bacterial artificial chromosome library of rice. The cloned 6,227-bp-long Reb gene is composed of six exons and five introns and is flanked by a 1.2-kb 5' promoter and a 1.2-kb 3' terminator region. The function of the Reb gen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.201411298

    authors： Yang D,Wu L,Hwang YS,Chen L,Huang N

    更新日期：2001-09-25 00:00:00

  • Induced transparency by interference or polarization.

    abstract：:Polarization of optical fields is a crucial degree of freedom in the all-optical analogue of electromagnetically induced transparency (EIT). However, the physical origins of EIT and polarization-induced phenomena have not been well distinguished, which can lead to confusion in associated applications such as slow ligh...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2012982118

    authors： Wang C,Jiang X,Sweeney WR,Hsu CW,Liu Y,Zhao G,Peng B,Zhang M,Jiang L,Stone AD,Yang L

    更新日期：2021-01-19 00:00:00

  • An engineered innate immune defense protects grapevines from Pierce disease.

    abstract：:We postulated that a synergistic combination of two innate immune functions, pathogen surface recognition and lysis, in a protein chimera would lead to a robust class of engineered antimicrobial therapeutics for protection against pathogens. In support of our hypothesis, we have engineered such a chimera to protect ag...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1116027109

    authors： Dandekar AM,Gouran H,Ibáñez AM,Uratsu SL,Agüero CB,McFarland S,Borhani Y,Feldstein PA,Bruening G,Nascimento R,Goulart LR,Pardington PE,Chaudhary A,Norvell M,Civerolo E,Gupta G

    更新日期：2012-03-06 00:00:00

  • Similarities and differences in signal transduction by interleukin 4 and interleukin 13: analysis of Janus kinase activation.

    abstract：:The cytokines interleukin (IL) 4 and IL-13 induce many of the same biological responses, including class switching to IgE and induction of major histocompatibility complex class II antigens and CD23 on human B cells. It has recently been shown that IL-4 induces the tyrosine phosphorylation of a 170-kDa protein, a subs...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.17.7681

    authors： Keegan AD,Johnston JA,Tortolani PJ,McReynolds LJ,Kinzer C,O'Shea JJ,Paul WE

    更新日期：1995-08-15 00:00:00

  • Plant genomics: more than food for thought.

    abstract：:In all but the poorest countries of South Asia and Africa, the supply and quality of food will rise to meet the demand. Biotechnology, accelerated by genomics, will create wealth for both producers and consumers by reducing the cost and increasing the quality of food. Famine and malnutrition in the poorest countries m...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.5.1986

    authors： Briggs SP

    更新日期：1998-03-03 00:00:00

  • p21(WAF1/Cip1) functions as a suppressor of malignant skin tumor formation and a determinant of keratinocyte stem-cell potential.

    abstract：:p21(WAF1/Cip1) is one of the best characterized downstream targets of p53, and the growth suppressing function of this cyclin-dependent kinase inhibitor is well established. However, whether p21 exerts a tumor-suppressing function of its own remains to be established. We report here that, similarly to loss of p53, dis...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.16.9089

    authors： Topley GI,Okuyama R,Gonzales JG,Conti C,Dotto GP

    更新日期：1999-08-03 00:00:00

  • Cbfβ governs osteoblast-adipocyte lineage commitment through enhancing β-catenin signaling and suppressing adipogenesis gene expression.

    abstract：:The mechanism underlying how transcription factors regulate mesenchymal stem cell lineage commitment remains unclear. To determine the role of core-binding factor subunit beta (Cbfβ) in osteoblast lineage commitment, we generated three mouse models by deleting Cbfβ at different osteoblast lineage stages. We demonstrat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1619294114

    authors： Wu M,Wang Y,Shao JZ,Wang J,Chen W,Li YP

    更新日期：2017-09-19 00:00:00

  • An algebraic description of stereochemical correspondence.

    abstract：:It is shown that a stereochemical correspondence between two molecular systems can be represented by a commuting diagram of the point groups and permutation groups involved. The effect of the diagrammatic condition on the mappings of the cosets, double cosets, subgroup lattice, and double coset algebra determined for ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.6.2385

    authors： Nourse JG

    更新日期：1975-06-01 00:00:00

  • Interleukin 10 inhibits macrophage microbicidal activity by blocking the endogenous production of tumor necrosis factor alpha required as a costimulatory factor for interferon gamma-induced activation.

    abstract：:Interleukin 10 (IL-10) inhibits interferon gamma-induced macrophage activation for cytotoxicity against larvae of the human parasite Schistosoma mansoni by suppressing production of the toxic effector molecule nitric oxide (NO). In this study, the mechanism of IL-10 action was identified as inhibition of endogenous tu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.18.8676

    authors： Oswald IP,Wynn TA,Sher A,James SL

    更新日期：1992-09-15 00:00:00

  • Structural flexibility at a major conserved antibody target on hepatitis C virus E2 antigen.

    abstract：:Hepatitis C virus (HCV) is a major cause of liver disease, affecting over 2% of the world's population. The HCV envelope glycoproteins E1 and E2 mediate viral entry, with E2 being the main target of neutralizing antibody responses. Structural investigations of E2 have produced templates for vaccine design, including t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1609780113

    authors： Kong L,Lee DE,Kadam RU,Liu T,Giang E,Nieusma T,Garces F,Tzarum N,Woods VL Jr,Ward AB,Li S,Wilson IA,Law M

    更新日期：2016-11-08 00:00:00

  • Molecular structure of monomorphic peptide fibrils within a kinetically trapped hydrogel network.

    abstract：:Most, if not all, peptide- and protein-based hydrogels formed by self-assembly can be characterized as kinetically trapped 3D networks of fibrils. The propensity of disease-associated amyloid-forming peptides and proteins to assemble into polymorphic fibrils suggests that cross-β fibrils comprising hydrogels may also ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1509313112

    authors： Nagy-Smith K,Moore E,Schneider J,Tycko R

    更新日期：2015-08-11 00:00:00

  • Functional anatomy of human eyeblink conditioning determined with regional cerebral glucose metabolism and positron-emission tomography.

    abstract：:Relative cerebral glucose metabolism was examined with positron-emission tomography (PET) as a measure of neuronal activation during performance of the classically conditioned eyeblink response in 12 young adult subjects. Each subject received three sessions: (i) a control session with PET scan in which unpaired prese...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 临床试验,杂志文章

    doi：10.1073/pnas.92.16.7500

    authors： Logan CG,Grafton ST

    更新日期：1995-08-01 00:00:00

  • Two transcripts encode rat cytochrome b5 reductase.

    abstract：:A cDNA expression library in lambda gt11 was screened with affinity-purified polyclonal anti-rat cytochrome b5 reductase antibodies. One positive clone out of 450,000 clones was isolated and found to be incomplete. This clone was used to rescreen the library, and a second, overlapping clone that contained the entire c...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.19.7246

    authors： Pietrini G,Carrera P,Borgese N

    更新日期：1988-10-01 00:00:00

  • A humid corridor across the Sahara for the migration of early modern humans out of Africa 120,000 years ago.

    abstract：:It is widely accepted that modern humans originated in sub-Saharan Africa approximately 150-200 thousand years ago (ka), but their route of dispersal across the currently hyperarid Sahara remains controversial. Given that the first modern humans north of the Sahara are found in the Levant approximately 120-90 ka, nort...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 历史文章,杂志文章

    doi：10.1073/pnas.0804472105

    authors： Osborne AH,Vance D,Rohling EJ,Barton N,Rogerson M,Fello N

    更新日期：2008-10-28 00:00:00

  • Phylogenetic profiles reveal evolutionary relationships within the "twilight zone" of sequence similarity.

    abstract：:Inferring evolutionary relationships among highly divergent protein sequences is a daunting task. In particular, when pairwise sequence alignments between protein sequences fall <25% identity, the phylogenetic relationships among sequences cannot be estimated with statistical certainty. Here, we show that phylogenetic...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0803860105

    authors： Chang GS,Hong Y,Ko KD,Bhardwaj G,Holmes EC,Patterson RL,van Rossum DB

    更新日期：2008-09-09 00:00:00

  • IKKβ is an IRF5 kinase that instigates inflammation.

    abstract：:The transcription factor interferon regulatory factor 5 (IRF5) is essential for the induction of inflammatory cytokines, but the mechanism by which IRF5 is activated is not well understood. Here we present evidence that the kinase IKKβ phosphorylates and activates IRF5 in response to stimulation in several inflammator...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1418516111

    authors： Ren J,Chen X,Chen ZJ

    更新日期：2014-12-09 00:00:00

  • Telomerase can act as a template- and RNA-independent terminal transferase.

    abstract：:Telomerase is a special reverse transcriptase that extends one strand of the telomere repeat by using a template embedded in an RNA subunit. Like other polymerases, telomerase is believed to use a pair of divalent metal ions (coordinated by a triad of aspartic acid residues) for catalyzing nucleotide addition. Here we...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0502252102

    authors： Lue NF,Bosoy D,Moriarty TJ,Autexier C,Altman B,Leng S

    更新日期：2005-07-12 00:00:00