Selective blockers of voltage-gated K+ channels depolarize human T lymphocytes: mechanism of the antiproliferative effect of charybdotoxin.

abstract：:Immobilization antigens of stock 51 of Paramecium tetraurelia were subjected to electrophoresis in NaDodSO(4)/polyacrylamide gels. Type A is estimated to have a molecular size of 300,000 daltons; H is estimated to be 288,000, D to be 280,000, E to be 270,000, B to be 253,000, and C to be 250,000. Poly(A)(+)RNAs have b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Preer JR,Preer LB,Rudman BM

更新日期：1981-11-01 00:00:00

abstract：:Mosquitoes are major disease vectors because most species must feed on blood from a vertebrate host to produce eggs. Blood feeding by the vector mosquito Aedes aegypti triggers the release of two neurohormones, ovary ecdysteroidogenic hormone (OEH) and insulin-like peptides (ILPs), which activate multiple processes re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vogel KJ,Brown MR,Strand MR

更新日期：2015-04-21 00:00:00

abstract：:A recently described experimental system for analyzing the mode of action of a morphogen gradient involves the in situ hybridization of sectioned tissue constructs. In these constructs, a source of activin signaling induces the transcription of several mesodermal genes in blastula animal caps, according to the positio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gurdon JB,Mitchell A,Ryan K

更新日期：1996-09-03 00:00:00

abstract：:A vitamin D(3) metabolite in intestine more polar than 25-hydroxy-vitamin D(3)(25-OH D(3)) has been detected by countercurrent distribution. The intestinal metabolite is found also after administration of labeled hydroxy D(3), indicating that it arises from vitamin D(3) via the intermediate 25-hydroxy derivative. The ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haussler MR,Boyce DW,Littledike ET,Rasmussen H

更新日期：1971-01-01 00:00:00

abstract：:We recently isolated an RNA polymerase II elongation factor from rat liver nuclei and found it to be homologous to the product of the human ELL gene, a frequent target for translocations in acute myeloid leukemia. To further our understanding of the possible role(s) of ELL in transcriptional regulation and human disea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shilatifard A,Duan DR,Haque D,Florence C,Schubach WH,Conaway JW,Conaway RC

更新日期：1997-04-15 00:00:00

abstract：:Mood disorders cause much suffering and are the single greatest cause of lost productivity worldwide. Although multiple medications, along with behavioral therapies, have proven effective for some individuals, millions of people lack an effective therapeutic option. A common serotonin (5-HT) transporter (5-HTT/SERT, S...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baganz NL,Horton RE,Calderon AS,Owens WA,Munn JL,Watts LT,Koldzic-Zivanovic N,Jeske NA,Koek W,Toney GM,Daws LC

更新日期：2008-12-02 00:00:00

abstract：:We describe a functional profiling strategy to identify and characterize subtypes of neurons present in a peripheral ganglion, which should be extendable to neurons in the CNS. In this study, dissociated dorsal-root ganglion neurons from mice were exposed to various pharmacological agents (challenge compounds), while ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teichert RW,Smith NJ,Raghuraman S,Yoshikami D,Light AR,Olivera BM

更新日期：2012-01-31 00:00:00

abstract：:Reactive oxygen species (ROS) play important roles in aging, inflammation, and cancer. Mitochondria are an important source of ROS; however, the spatiotemporal ROS events underlying oxidative cellular damage from dysfunctional mitochondria remain unresolved. To this end, we have developed and validated a chemoptogenet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qian W,Kumar N,Roginskaya V,Fouquerel E,Opresko PL,Shiva S,Watkins SC,Kolodieznyi D,Bruchez MP,Van Houten B

更新日期：2019-09-10 00:00:00

abstract：:Human pluripotent stem cells have the potential to provide comprehensive model systems for the earliest stages of human ontogenesis. To serve in this capacity, these cells must undergo a targeted, stepwise differentiation process that follows a normal developmental timeline. Here we demonstrate the ability of both hum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meyer JS,Shearer RL,Capowski EE,Wright LS,Wallace KA,McMillan EL,Zhang SC,Gamm DM

更新日期：2009-09-29 00:00:00

abstract：:Understanding the ecological impacts of climate change is a crucial challenge of the twenty-first century. There is a clear lack of general rules regarding the impacts of global warming on biota. Here, we present a metaanalysis of the effect of climate change on body size of ectothermic aquatic organisms (bacteria, ph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daufresne M,Lengfellner K,Sommer U

更新日期：2009-08-04 00:00:00

abstract：:We have determined the general constraints that govern sequence divergence in proteins that retain entirely, or very largely, the same structure and function. To do this we collected data from three different groups of orthologous sequences: those found in humans and mice, in humans and chickens, and in Escherichia co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sasidharan R,Chothia C

更新日期：2007-06-12 00:00:00

abstract：:In skeletal muscle, dihydropyridine receptors are functionally coupled to ryanodine receptors of the sarcoplasmic reticulum in triadic or diadic junctional complexes. In cardiac muscle direct physical or functional couplings have not been demonstrated. We have tested the hypothesis of functional coupling of L-type Ca2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sham JS,Cleemann L,Morad M

更新日期：1995-01-03 00:00:00

abstract：:What are the forces that shape the structure of prokaryotic genomes: the order of genes, their proximity, and their orientation? Coregulation and coordinated horizontal gene transfer are believed to promote the proximity of functionally related genes and the formation of operons. However, forces that influence the str...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kolesov G,Wunderlich Z,Laikova ON,Gelfand MS,Mirny LA

更新日期：2007-08-28 00:00:00

abstract：:In many naturally occurring habitats, bacteria live in micrometer-size confined spaces. Although bacterial growth and motility in such constrictions is of great interest to fields as varied as soil microbiology, water purification, and biomedical research, quantitative studies of the effects of confinement on bacteria...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Männik J,Driessen R,Galajda P,Keymer JE,Dekker C

更新日期：2009-09-01 00:00:00

abstract：:When assembled into 13--19S particles, the reovirus nonstructural protein sigma-NS selectively binds single-stranded RNAs. Sedimentation analyses combined with binding to nitrocellulose membrane filters showed that 1--2 pmol of reovirus mRNAs from the large, medium, or small size classes saturated in vitro the binding...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stamatos NM,Gomatos PJ

更新日期：1982-06-01 00:00:00

abstract：:Senescent cell antigen (SCA), an aging antigen, is a protein that appears on old cells and marks them for removal by the immune system in mammals. It is derived from band 3, a ubiquitous membrane transport protein found in diverse cell types and tissues. We have used synthetic peptides to identify aging antigenic site...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kay MM,Marchalonis JJ,Hughes J,Watanabe K,Schluter SF

更新日期：1990-08-01 00:00:00

abstract：:Morphine produces analgesia at opiate receptors expressed in nociceptive circuits. mu, delta, and kappa opiate receptor subtypes are expressed in circuits that can modulate nociception and receive inputs from endogenous opioid neuropeptide ligands. The roles played by each receptor subtype in nociceptive processing in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sora I,Takahashi N,Funada M,Ujike H,Revay RS,Donovan DM,Miner LL,Uhl GR

更新日期：1997-02-18 00:00:00

abstract：:To use recombinant DNA technology to functionally analyze mutations introduced into cloned eukaryotic genes, a rapid procedure is necessary to assay the steps along the gene expression pathway. Since cloned rat insulin genes are not transcribed efficiently after transfection into various cell lines, I have asked wheth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lomedico PT

更新日期：1982-10-01 00:00:00

abstract：:Toll-like receptors (TLRs) are expressed on innate immune cells and trigger inflammation upon detection of pathogens and host tissue injury. TLR-mediated proinflammatory-signaling pathways are counteracted by partially characterized anti-inflammatory mechanisms that prevent exaggerated inflammation and host tissue dam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou J,Wu R,High AA,Slaughter CA,Finkelstein D,Rehg JE,Redecke V,Häcker H

更新日期：2011-11-01 00:00:00

abstract：:Increasing global temperature and sea-level rise have led to concern about expansions in the distribution and prevalence of complex-lifecycle parasites (CLPs). Indeed, numerous environmental variables can influence the infectivity and reproductive output of many pathogens. Digenean trematodes are CLPs with intermediat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huntley JW,Fürsich FT,Alberti M,Hethke M,Liu C

更新日期：2014-12-23 00:00:00

abstract：:A cardinal symptom of major depressive disorder (MDD) is the disruption of circadian patterns. However, to date, there is no direct evidence of circadian clock dysregulation in the brains of patients who have MDD. Circadian rhythmicity of gene expression has been observed in animals and peripheral human tissues, but i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li JZ,Bunney BG,Meng F,Hagenauer MH,Walsh DM,Vawter MP,Evans SJ,Choudary PV,Cartagena P,Barchas JD,Schatzberg AF,Jones EG,Myers RM,Watson SJ Jr,Akil H,Bunney WE

更新日期：2013-06-11 00:00:00

abstract：:Xenotropic and polytropic murine leukemia viruses (X-MLVs and P-MLVs) cross-interfere to various extents in non-mouse species and in wild Asian mice, suggesting that they might use a common receptor for infection. Consistent with this hypothesis, the susceptibility of some wild mice to X-MLVs has been mapped to the P-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tailor CS,Nouri A,Lee CG,Kozak C,Kabat D

更新日期：1999-02-02 00:00:00

abstract：:We use a three-dimensional, higher-order ice flow model and a realistic initial condition to simulate dynamic perturbations to the Greenland ice sheet during the last decade and to assess their contribution to sea level by 2100. Starting from our initial condition, we apply a time series of observationally constrained...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Price SF,Payne AJ,Howat IM,Smith BE

更新日期：2011-05-31 00:00:00

abstract：:The [PSI+] prion is a self-propagating amyloid of the translation termination factor, Sup35p, of Saccharomyces cerevisiae. The N-terminal 253 residues (NM) of this 685-residue protein normally function in regulating mRNA turnover but spontaneously form infectious amyloid in vitro. We converted the three Ile residues i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gorkovskiy A,Thurber KR,Tycko R,Wickner RB

更新日期：2014-10-28 00:00:00

abstract：:We described previously a mutation in maize, hcf106, caused by the insertion of a Mu1 transposon. When the Mu transposon system is in an active phase, hcf106 conditions a nonphotosynthetic, pale green phenotype. However, when the Mu system is inactive (a state correlated with hypermethylation of Mu elements), the plan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barkan A,Martienssen RA

更新日期：1991-04-15 00:00:00

abstract：:Carcinosarcomas (CSs) of the uterus and ovary are highly aggressive neoplasms containing both carcinomatous and sarcomatous elements. We analyzed the mutational landscape of 68 uterine and ovarian CSs by whole-exome sequencing. We also performed multiregion whole-exome sequencing comprising two carcinoma and sarcoma s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao S,Bellone S,Lopez S,Thakral D,Schwab C,English DP,Black J,Cocco E,Choi J,Zammataro L,Predolini F,Bonazzoli E,Bi M,Buza N,Hui P,Wong S,Abu-Khalaf M,Ravaggi A,Bignotti E,Bandiera E,Romani C,Todeschini P,Tas

更新日期：2016-10-25 00:00:00

abstract：:Here we describe two mammalian transcription factors selectively expressed in the central nervous system. Both proteins, neuronal PAS domain protein (NPAS) 1 and NPAS2, are members of the basic helix-loop-helix-PAS family of transcription factors. cDNAs encoding mouse and human forms of NPAS1 and NPAS2 have been isola...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou YD,Barnard M,Tian H,Li X,Ring HZ,Francke U,Shelton J,Richardson J,Russell DW,McKnight SL

更新日期：1997-01-21 00:00:00

abstract：:Unit electrical activity was recorded from single neurons in the isolated lumbo-sacral spinal cord of 14- to 19-day chick embryos, in situ. Spinal cord transection was combined with transection of all lumbo-sacral dorsal roots. The spontaneous discharge of cells is confined, for the most part, to the lower two thirds ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharma SC,Provine RR,Hamburger V,Sandel TT

更新日期：1970-05-01 00:00:00

abstract：:The kinetics and order of rearrangements in the gene complex encoding T-cell-receptor beta chains were studied by Southern blot hybridization in a collection of hybridomas derived from fetal thymocytes at various stages of ontogeny (day 14 to day 17). Our results show a steady increase in the frequency of rearranged b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Born W,Yagüe J,Palmer E,Kappler J,Marrack P

更新日期：1985-05-01 00:00:00

abstract：:A plasmid that consists of an 812-base-pair segment containing the replication origin of plasmid ColE1 and of a 1240-base-pair segment containing a beta-lactamase gene has been constructed. The plasmid DNA has three principal sites where transcription is initiated in vitro. One is located in the ColE1 segment 555 nucl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Itoh T,Tomizawa J

更新日期：1980-05-01 00:00:00