Definition of a physiologic aging autoantigen by using synthetic peptides of membrane protein band 3: localization of the active antigenic sites.

abstract：:Cholinergic pathways serve important functions in learning and memory processes, and deficits in cholinergic transmission occur in Alzheimer disease (AD). A subset of muscarinic cholinergic receptors are linked to G-proteins that activate phospholipase C, resulting in the liberation of inositol trisphosphate and Ca2+ ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelly JF,Furukawa K,Barger SW,Rengen MR,Mark RJ,Blanc EM,Roth GS,Mattson MP

更新日期：1996-06-25 00:00:00

abstract：:Analysis of variations in the concentrations or structures of biomolecules (e.g., mRNAs, proteins, peptides, natural products) that occur either naturally or in response to environmental or genetic perturbations can provide important insight into complex biological processes. Many biological samples are mixtures that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yao S,Anex DS,Caldwell WB,Arnold DW,Smith KB,Schultz PG

更新日期：1999-05-11 00:00:00

abstract：:Interleukin 2 (IL-2) plays a critical role in the growth and differentiation of lymphoid cells. The IL-2 signal is delivered intracellularly by the IL-2 receptor beta chain (IL-2R beta); however, the mechanism by which the signal reaches the nucleus remains unclear. In this study, we demonstrate the rapid activation o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hatakeyama M,Kawahara A,Mori H,Shibuya H,Taniguchi T

更新日期：1992-03-15 00:00:00

abstract：:A culture of mouse A9 cells yielded clones of both high and low malignancy. Confirmation of the differences in malignancy of the clones was obtained after their hybridization with subclones of YACIR a lymphoma induced by Moloney leukemia virus. Low malignant A9 clones suppressed the malignancy of the YACIR partner in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clements GB,Fenyö EM,Klein G

更新日期：1976-06-01 00:00:00

abstract：:The requirement and influence of the peripheral nervous system on tissue replacement in mammalian appendages remain largely undefined. To explore this question, we have performed genetic lineage tracing and clonal analysis of individual cells of mouse hind limb tissues devoid of nerve supply during regeneration of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rinkevich Y,Montoro DT,Muhonen E,Walmsley GG,Lo D,Hasegawa M,Januszyk M,Connolly AJ,Weissman IL,Longaker MT

更新日期：2014-07-08 00:00:00

abstract：:Semaphorin 4D (sema4D; CD100) is an integral membrane protein and the ligand for two receptors, CD72 and plexin-B1. Soluble sema4D has been shown to evoke angiogenic responses from endothelial cells and impair monocyte migration, but the origin of soluble sema4D, particularly at sites of vascular injury, has been uncl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu L,Bergmeier W,Wu J,Jiang H,Stalker TJ,Cieslak M,Fan R,Boumsell L,Kumanogoh A,Kikutani H,Tamagnone L,Wagner DD,Milla ME,Brass LF

更新日期：2007-01-30 00:00:00

abstract：:The pathways that allow quiescent disseminated cancer cells to survive during prolonged dormancy periods are unknown. Here, we identify the transcription factor ATF6alpha as a pivotal survival factor for quiescent but not proliferative squamous carcinoma cells. ATF6alpha is essential for the adaptation of dormant cell...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schewe DM,Aguirre-Ghiso JA

更新日期：2008-07-29 00:00:00

abstract：:EF-hand Ca(II) binding sites share a conserved architecture and are prevalent in Ca(II) signaling pathways. The ion binding kinetics of these sites are carefully tuned to provide the physiologically appropriate activation and inactivation time scales. Here we examine kinetic tuning by the side chain at the ninth posit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renner M,Danielson MA,Falke JJ

更新日期：1993-07-15 00:00:00

abstract：:Marine viruses constitute a major ecological and evolutionary driving force in the marine ecosystems. However, their dispersal mechanisms remain underexplored. Here we follow the dynamics of Emiliania huxleyi viruses (EhV) that infect the ubiquitous, bloom-forming phytoplankton E. huxleyi and show that EhV are emitted...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharoni S,Trainic M,Schatz D,Lehahn Y,Flores MJ,Bidle KD,Ben-Dor S,Rudich Y,Koren I,Vardi A

更新日期：2015-05-26 00:00:00

abstract：:Diversity in 39 HLA-A, -B, and -C molecules is derived from 20 amino acid positions of high variability and 71 positions of low variability. Variation in the structurally homologous alpha 1 and alpha 2 domains is distinct and may correlate with partial segregation of peptide and T-cell receptor binding functions. Comp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parham P,Lomen CE,Lawlor DA,Ways JP,Holmes N,Coppin HL,Salter RD,Wan AM,Ennis PD

更新日期：1988-06-01 00:00:00

abstract：:Conventional markets can underprovide ecosystem services. Deliberate creation of a market for ecosystem services [e.g., a payments for ecosystem services (PES) scheme] can close the gap. The new ecosystem service market alters behaviors and quantities of ecosystem service provided and reveals prices for the ecosystems...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adamowicz W,Calderon-Etter L,Entem A,Fenichel EP,Hall JS,Lloyd-Smith P,Ogden FL,Regina JA,Rouhi Rad M,Stallard RF

更新日期：2019-03-19 00:00:00

abstract：:Intracellular electrophysiological recordings were obtained from a specialized class of "starburst" amacrine cells by using an isolated superperfused retina-eyecup preparation of the rabbit. These cells were injected intracellularly with horseradish peroxidase and identified with light microscopy. A computer-controlle...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller RF,Bloomfield SA

更新日期：1983-05-01 00:00:00

abstract：:Prenatal stress exposure is associated with risk for psychiatric disorders later in life. This may be mediated in part via enhanced exposure to glucocorticoids (GCs), which are known to impact neurogenesis. We aimed to identify molecular mediators of these effects, focusing on long-lasting epigenetic changes. In a hum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Provençal N,Arloth J,Cattaneo A,Anacker C,Cattane N,Wiechmann T,Röh S,Ködel M,Klengel T,Czamara D,Müller NS,Lahti J,PREDO team.,Räikkönen K,Pariante CM,Binder EB

更新日期：2020-09-22 00:00:00

abstract：:We report on the expression of thymidine kinase (tk) activity in synchronized populations of mouse cells that have been transformed to the tk+ phenotype with purified DNA from various sources or with metaphase chromosomes from human cells. The viral (herpes) tk gene is constitutively expressed but, in all other cases ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schlosser CA,Steglich C,deWet JR,Scheffler IE

更新日期：1981-02-01 00:00:00

abstract：:With the completion of genome sequences of major model organisms, increasingly sophisticated genetic tools are necessary for investigating the complex and coordinated functions of genes. Here we describe a genetic manipulation system termed "genomic engineering" in Drosophila. Genomic engineering is a 2-step process t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang J,Zhou W,Dong W,Watson AM,Hong Y

更新日期：2009-05-19 00:00:00

abstract：:Accumulation of (45)Ca(++) was found to occur in membrane vesicles of E. coli prepared by lysis with a French pressure cell. The uptake occurs by active transport, requiring an energy source. Substrates of the electron transport chain, including D-lactate, reduced phenazine methosulfate, and NADH, stimulated accumulat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rosen BP,McClees JS

更新日期：1974-12-01 00:00:00

abstract：:The stability of an immunogen against enzymatic degradation is considered an important factor for the design of synthetic vaccines. For our studies, we have selected an epitope from the tandem-repeat unit of the high-molecular-weight MUC2 mucin glycoprotein, which can be underglycosylated in case of colon cancer. In t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tugyi R,Uray K,Iván D,Fellinger E,Perkins A,Hudecz F

更新日期：2005-01-11 00:00:00

abstract：:By commandeering cellular translation initiation factors, or destroying those dispensable for viral mRNA translation, viruses often suppress host protein synthesis. In contrast, cellular protein synthesis proceeds in human cytomegalovirus (HCMV)-infected cells, forcing viral and cellular mRNAs to compete for limiting ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McKinney C,Perez C,Mohr I

更新日期：2012-04-10 00:00:00

abstract：:Infection of mice with sporozoites of Plasmodium berghei or Plasmodium yoelii has been used extensively to evaluate liver-stage protection by candidate preerythrocytic malaria vaccines. Unfortunately, repeated success of such vaccines in mice has not translated readily to effective malaria vaccines in humans. Thus, mi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmidt NW,Podyminogin RL,Butler NS,Badovinac VP,Tucker BJ,Bahjat KS,Lauer P,Reyes-Sandoval A,Hutchings CL,Moore AC,Gilbert SC,Hill AV,Bartholomay LC,Harty JT

更新日期：2008-09-16 00:00:00

abstract：:CD31 is an Ig-like molecule expressed by leukocytes and endothelial cells with an established role in the regulation of leukocyte trafficking. Despite genetic deletion of CD31 being associated with exacerbation of T cell-mediated autoimmunity, the contribution of this molecule to T-cell responses is largely unknown. H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma L,Mauro C,Cornish GH,Chai JG,Coe D,Fu H,Patton D,Okkenhaug K,Franzoso G,Dyson J,Nourshargh S,Marelli-Berg FM

更新日期：2010-11-09 00:00:00

abstract：:A sensitive test for kinetic unfolding intermediates in ribonuclease A (EC 3.1.27.5) is performed under conditions where the enzyme unfolds slowly (10 degrees C, pH 8.0, 4.5 M guanidinium chloride). Exchange of peptide NH protons (2H-1H) is used to monitor structural opening of individual hydrogen bonds during unfoldi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kiefhaber T,Baldwin RL

更新日期：1995-03-28 00:00:00

abstract：:Purified dihydropyridine-sensitive calcium channels from rabbit skeletal muscle were reconstituted into phosphatidylcholine vesicles to evaluate the effect of phosphorylation by cyclic AMP-dependent protein kinase (PK-A) on their function. Both the rate and extent of 45Ca2+ uptake into vesicles containing reconstitute...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nunoki K,Florio V,Catterall WA

更新日期：1989-09-01 00:00:00

abstract：:A biological membrane is conceptualized as a system in which membrane proteins are naturally matched to the equilibrium thickness of the lipid bilayer. Cholesterol, in addition to lipid composition, has been suggested to be a major regulator of bilayer thickness in vivo because measurements in vitro have shown that ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitra K,Ubarretxena-Belandia I,Taguchi T,Warren G,Engelman DM

更新日期：2004-03-23 00:00:00

abstract：:Tomato (Solanum lycopersicum) fruit accumulate the red carotenoid pigment lycopene. The recessive mutation yellow-flesh (locus r) in tomato eliminates fruit carotenoids by disrupting the activity of the fruit-specific phytoene synthase (PSY1), the first committed step in the carotenoid biosynthesis pathway. Fruits of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kachanovsky DE,Filler S,Isaacson T,Hirschberg J

更新日期：2012-11-13 00:00:00

abstract：:Oxidative stress is known to cause tumorigenesis through induction of DNA and lipid damage. It also promotes cancer progression through a largely unknown mechanism. Sulfiredoxin (Srx) is a novel oxidative stress-induced antioxidant protein whose function in tumorigenesis and cancer progression has not been well studie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wei Q,Jiang H,Xiao Z,Baker A,Young MR,Veenstra TD,Colburn NH

更新日期：2011-04-26 00:00:00

abstract：:The central administration of the fatty acid synthase (FAS) inhibitor, C75, rapidly suppresses the expression of orexigenic neuropeptides [neuropeptide Y (NPY) and agouti-related protein (AgRP)] and activates expression of anorexigenic neuropeptides [proopiomelanocortin (POMC) and cocaine- and amphetamine-regulated tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu Z,Cha SH,van Haasteren G,Wang J,Lane MD

更新日期：2005-03-15 00:00:00

abstract：:Disulfide bonds have only rarely been found in intracellular proteins. That pattern is consistent with the chemically reducing environment inside the cells of well-studied organisms. However, recent experiments and new calculations based on genomic data of archaea provide striking contradictions to this pattern. Our r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mallick P,Boutz DR,Eisenberg D,Yeates TO

更新日期：2002-07-23 00:00:00

abstract：:Histone acetylation is a major epigenetic control mechanism that is tightly linked to the promotion of gene expression. Histone acetylation levels are balanced through the opposing activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). Arabidopsis HDAC genes (AtHDACs) compose a large gene fa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim YJ,Wang R,Gao L,Li D,Xu C,Mang H,Jeon J,Chen X,Zhong X,Kwak JM,Mo B,Xiao L,Chen X

更新日期：2016-12-20 00:00:00

abstract：:As new disease threats arise and existing pathogens grow resistant to conventional interventions, attention increasingly focuses on the development of vaccines to induce protective immune responses. Given their admirable safety records, protein subunit vaccines are attractive for widespread immunization, but their dis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen DJ,Osterrieder N,Metzger SM,Buckles E,Doody AM,DeLisa MP,Putnam D

更新日期：2010-02-16 00:00:00

abstract：:Molecular dynamics (MD) simulations provide essential information about the thermodynamics and dynamics of proteins. To construct the free-energy surface from equilibrium trajectories, it is necessary to group the individual snapshots in a meaningful way. The inherent structures (IS) are shown to provide an appropriat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rao F,Karplus M

更新日期：2010-05-18 00:00:00