Abstract:
:Disulfide bonds have only rarely been found in intracellular proteins. That pattern is consistent with the chemically reducing environment inside the cells of well-studied organisms. However, recent experiments and new calculations based on genomic data of archaea provide striking contradictions to this pattern. Our results indicate that the intracellular proteins of certain hyperthermophilic archaea, especially the crenarchaea Pyrobaculum aerophilum and Aeropyrum pernix, are rich in disulfide bonds. This finding implicates disulfide bonding in stabilizing many thermostable proteins and points to novel chemical environments inside these microbes. These unexpected results illustrate the wealth of biochemical insights available from the growing reservoir of genomic data.
journal_name
Proc Natl Acad Sci U S Aauthors
Mallick P,Boutz DR,Eisenberg D,Yeates TOdoi
10.1073/pnas.142310499keywords:
subject
Has Abstractpub_date
2002-07-23 00:00:00pages
9679-84issue
15eissn
0027-8424issn
1091-6490pii
142310499journal_volume
99pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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