当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Dimerization of a viral SET protein endows its function.

Dimerization of a viral SET protein endows its function.

Abstract：

:Histone modifications are regarded as the most indispensible phenomena in epigenetics. Of these modifications, lysine methylation is of the greatest complexity and importance as site- and state-specific lysine methylation exerts a plethora of effects on chromatin structure and gene transcription. Notably, paramecium bursaria chlorella viruses encode a conserved SET domain methyltransferase, termed vSET, that functions to suppress host transcription by methylating histone H3 at lysine 27 (H3K27), a mark for eukaryotic gene silencing. Unlike mammalian lysine methyltransferases (KMTs), vSET functions only as a dimer, but the underlying mechanism has remained elusive. In this study, we demonstrate that dimeric vSET operates with negative cooperativity between the two active sites and engages in H3K27 methylation one site at a time. New atomic structures of vSET in the free form and a ternary complex with S-adenosyl homocysteine and a histone H3 peptide and biochemical analyses reveal the molecular origin for the negative cooperativity and explain the substrate specificity of H3K27 methyltransferases. Our study suggests a "walking" mechanism, by which vSET acts all by itself to globally methylate host H3K27, which is accomplished by the mammalian EZH2 KMT only in the context of the Polycomb repressive complex.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Wei H,Zhou MM

doi

10.1073/pnas.1009911107

subject

Has Abstract

pub_date

2010-10-26 00:00:00

pages

18433-8

issue

43

eissn

0027-8424

issn

1091-6490

pii

1009911107

journal_volume

107

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Global warming accelerates uptake of atmospheric mercury in regions experiencing glacier retreat.

    abstract：:As global climate continues to warm, melting of glaciers releases a large quantity of mercury (Hg) originally locked in ice into the atmosphere and downstream ecosystems. Here, we show an opposite process that captures atmospheric Hg through glacier-to-vegetation succession. Our study using stable isotope techniques a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1906930117

    authors： Wang X,Luo J,Yuan W,Lin CJ,Wang F,Liu C,Wang G,Feng X

    更新日期：2020-01-28 00:00:00

  • Growth of a pure population of mouse mast cells in vitro with conditioned medium derived from concanavalin A-stimulated splenocytes.

    abstract：:A pure population of mast cells was obtained after 14 days of culturing mouse bone marrow cells in the presence of medium derived from concanavalin A-stimulated mouse spleen cells. The cells were characterized as mast cells by their morphologic appearance and histologic staining, by their histamine content (450 ng per...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.4.2559

    authors： Razin E,Cordon-Cardo C,Good RA

    更新日期：1981-04-01 00:00:00

  • Antagonistic role of vega1 and bozozok/dharma homeobox genes in organizer formation.

    abstract：:During zebrafish development, zygotic gene expression initiated at the midblastula transition converts maternal information on embryo polarity into a transcriptional read-out. Expression of a homeobox gene, vega1, is activated at midblastula transition in all blastomeres, but is down-regulated dorsally before gastrula...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.22.12121

    authors： Kawahara A,Wilm T,Solnica-Krezel L,Dawid IB

    更新日期：2000-10-24 00:00:00

  • Localization of the zinc binding site of aspartate transcarbamoylase in the regulatory subunit.

    abstract：:Aspartate transcarbamoylase (EC 2.1.3.2) from Escherichia coli contains six zinc ions per molecule. Renaturation studies of this allosteric enzyme and its isolated subunits show that the metal binding site is in the regulatory polypeptide chain. Ultraviolet difference spectra of the cadmium and zinc derivatives have b...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.68.5.1019

    authors： Rosenbusch JP,Weber K

    更新日期：1971-05-01 00:00:00

  • Cytolytic and antibacterial activity of synthetic peptides derived from amoebapore, the pore-forming peptide of Entamoeba histolytica.

    abstract：:The pore-forming peptide amoebapore is considered part of the cytolytic armament of pathogenic Entamoeba histolytica. Amoebapore is composed of 77 amino acid residues arranged in four alpha-helical domains. For structure-function analysis, synthetic peptides were constructed corresponding to these four domains: H1 (re...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.7.2602

    authors： Leippe M,Andrä J,Müller-Eberhard HJ

    更新日期：1994-03-29 00:00:00

  • Properties of RNA transcriptase in reovirus subviral particles.

    abstract：:Subviral particles containing reovirus RNA transcriptase have been isolated from extracts of virus-infected mouse fibroblast cells. The purified particles which lacked the outer protein capsomeres of the mature virion had a buoyant density of 1.43-1.44 g/ml in CsCl and contained all of the double-stranded RNA genome o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.66.3.890

    authors： Levin DH,Mendelsohn N,Schonberg M,Klett H,Silverstein S,Kapuler AM,Acs G

    更新日期：1970-07-01 00:00:00

  • Processing of precursors of 21S ribosomal RNA from yeast mitochondria.

    abstract：:The transcription and processing of mitochondrial 21S rRNA in a petite strain of Saccharomyces cerevisiae has been examined by electron microscopic analysis of R-loop hybrids and by hybridization of labeled mitochondrial DNA probes to RNA transferred to diazobenzyloxymethyl paper. We have shown the presence of a large...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.3.1417

    authors： Merten S,Synenki RM,Locker J,Christianson T,Rabinowitz M

    更新日期：1980-03-01 00:00:00

  • Ecological and socioeconomic effects of China's policies for ecosystem services.

    abstract：:To address devastating environmental crises and to improve human well-being, China has been implementing a number of national policies on payments for ecosystem services. Two of them, the Natural Forest Conservation Program (NFCP) and the Grain to Green Program (GTGP), are among the biggest programs in the world becau...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,评审

    doi：10.1073/pnas.0706436105

    authors： Liu J,Li S,Ouyang Z,Tam C,Chen X

    更新日期：2008-07-15 00:00:00

  • Natural and synthetic prion structure from X-ray fiber diffraction.

    abstract：:A conformational isoform of the mammalian prion protein (PrP(Sc)) is the sole component of the infectious pathogen that causes the prion diseases. We have obtained X-ray fiber diffraction patterns from infectious prions that show cross-beta diffraction: meridional intensity at 4.8 A resolution, indicating the presence...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0909006106

    authors： Wille H,Bian W,McDonald M,Kendall A,Colby DW,Bloch L,Ollesch J,Borovinskiy AL,Cohen FE,Prusiner SB,Stubbs G

    更新日期：2009-10-06 00:00:00

  • Optogenetic activation of dopamine neurons in the ventral tegmental area induces reanimation from general anesthesia.

    abstract：:Dopamine (DA) promotes wakefulness, and DA transporter inhibitors such as dextroamphetamine and methylphenidate are effective for increasing arousal and inducing reanimation, or active emergence from general anesthesia. DA neurons in the ventral tegmental area (VTA) are involved in reward processing, motivation, emoti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1614340113

    authors： Taylor NE,Van Dort CJ,Kenny JD,Pei J,Guidera JA,Vlasov KY,Lee JT,Boyden ES,Brown EN,Solt K

    更新日期：2016-11-08 00:00:00

  • E1A-dependent trans-activation of the human MYC promoter is mediated by the E2F factor.

    abstract：:E2F is a cellular transcription factor that binds to two sites in the adenovirus E2 promoter. Previous experiments have implicated E2F in the E1A-dependent transactivation of the E2 gene since levels of active E2F increase markedly during adenovirus infection in parallel with the increase in E2 transcription, and an E...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.10.3594

    authors： Hiebert SW,Lipp M,Nevins JR

    更新日期：1989-05-01 00:00:00

  • Differential development of large-cell neuroendocrine or small-cell lung carcinoma upon inactivation of 4 tumor suppressor genes.

    abstract：:High-grade neuroendocrine lung malignancies (large-cell neuroendocrine cell carcinoma, LCNEC, and small-cell lung carcinoma, SCLC) are among the most deadly lung cancer conditions with no optimal clinical management. The biological relationships between SCLC and LCNEC are still largely unknown and a current matter of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1821745116

    authors： Lázaro S,Pérez-Crespo M,Lorz C,Bernardini A,Oteo M,Enguita AB,Romero E,Hernández P,Tomás L,Morcillo MÁ,Paramio JM,Santos M

    更新日期：2019-10-29 00:00:00

  • Ca2+-induced inhibition of the cardiac Ca2+ channel depends on calmodulin.

    abstract：:Ca2+-induced inhibition of alpha1C voltage-gated Ca2+ channels is a physiologically important regulatory mechanism that shortens the mean open time of these otherwise long-lasting high-voltage-activated channels. The mechanism of action of Ca2+ has been a matter of some controversy, as previous studies have proposed t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.5.2435

    authors： Qin N,Olcese R,Bransby M,Lin T,Birnbaumer L

    更新日期：1999-03-02 00:00:00

  • Direct demonstration of a correspondence between the dopamine islands and acetylcholinesterase patches in the developing striatum.

    abstract：:The distribution of dopamine-containing processes in the striatum of fetal and neonatal cats was studied by immunohistochemical and glyoxylic acid histofluorescence methods and compared to the distribution of acetylcholinesterase (acetylcholine acetylhydrolase, EC 3.1.1.7) observed by thiocholine histochemistry in the...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.9.5871

    authors： Graybiel AM,Pickel VM,Joh TH,Reis DJ,Ragsdale CW Jr

    更新日期：1981-09-01 00:00:00

  • A single amino acid difference in the host APOBEC3G protein controls the primate species specificity of HIV type 1 virion infectivity factor.

    abstract：:The HIV type 1 (HIV-1) virion infectivity factor (Vif) protein blocks the action of the host defense factor APOBEC3G in human cells, thereby allowing release of infectious virions, but fails to inhibit similar APOBEC3G proteins present in some simian cells. Conversely, the Vif protein encoded by the African green monk...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0307713101

    authors： Bogerd HP,Doehle BP,Wiegand HL,Cullen BR

    更新日期：2004-03-16 00:00:00

  • A yeast-like mRNA capping apparatus in Plasmodium falciparum.

    abstract：:Analysis of the mRNA capping apparatus of the malaria parasite Plasmodium falciparum illuminates an evolutionary connection to fungi rather than metazoans. We show that P. falciparum encodes separate RNA guanylyltransferase (Pgt1) and RNA triphosphatase (Prt1) enzymes and that the triphosphatase component is a member ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.061636198

    authors： Ho CK,Shuman S

    更新日期：2001-03-13 00:00:00

  • Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein.

    abstract：:Seasonal epidemics and periodic worldwide pandemics caused by influenza A viruses are of continuous concern. The viral nonstructural (NS1) protein is a multifunctional virulence factor that antagonizes several host innate immune defenses during infection. NS1 also directly stimulates class IA phosphoinositide 3-kinase...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0910715107

    authors： Hale BG,Kerry PS,Jackson D,Precious BL,Gray A,Killip MJ,Randall RE,Russell RJ

    更新日期：2010-02-02 00:00:00

  • Structure of the full-length Shaker potassium channel Kv1.2 by normal-mode-based X-ray crystallographic refinement.

    abstract：:Voltage-dependent potassium channels (Kv) are homotetramers composed of four voltage sensors and one pore domain. Because of high-level structural flexibility, the first mammalian Kv structure, Kv1.2 at 2.9 A, has about 37% molecular mass of the transmembrane portion not resolved. In this study, by applying a novel no...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1000142107

    authors： Chen X,Wang Q,Ni F,Ma J

    更新日期：2010-06-22 00:00:00

  • On artifacts in single-molecule force spectroscopy.

    abstract：:In typical force spectroscopy experiments, a small biomolecule is attached to a soft polymer linker that is pulled with a relatively large bead or cantilever. At constant force, the total extension stochastically changes between two (or more) values, indicating that the biomolecule undergoes transitions between two (o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1519633112

    authors： Cossio P,Hummer G,Szabo A

    更新日期：2015-11-17 00:00:00

  • Identification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein).

    abstract：:VASP (vasodilator-stimulated phosphoprotein), an established substrate of cAMP- and cGMP-dependent protein kinases in vitro and in living cells, is associated with focal adhesions, microfilaments, and membrane regions of high dynamic activity. Here, the identification of an 83-kDa protein (p83) that specifically binds...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.17.7956

    authors： Reinhard M,Jouvenal K,Tripier D,Walter U

    更新日期：1995-08-15 00:00:00

  • Poly(A) binding protein abundance regulates eukaryotic translation initiation factor 4F assembly in human cytomegalovirus-infected cells.

    abstract：:By commandeering cellular translation initiation factors, or destroying those dispensable for viral mRNA translation, viruses often suppress host protein synthesis. In contrast, cellular protein synthesis proceeds in human cytomegalovirus (HCMV)-infected cells, forcing viral and cellular mRNAs to compete for limiting ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1202829109

    authors： McKinney C,Perez C,Mohr I

    更新日期：2012-04-10 00:00:00

  • Single-molecule imaging reveals target-search mechanisms during DNA mismatch repair.

    abstract：:The ability of proteins to locate specific targets among a vast excess of nonspecific DNA is a fundamental theme in biology. Basic principles governing these search mechanisms remain poorly understood, and no study has provided direct visualization of single proteins searching for and engaging target sites. Here we us...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1211364109

    authors： Gorman J,Wang F,Redding S,Plys AJ,Fazio T,Wind S,Alani EE,Greene EC

    更新日期：2012-11-06 00:00:00

  • Cytosolic Fc receptor TRIM21 inhibits seeded tau aggregation.

    abstract：:Alzheimer's disease (AD) and other neurodegenerative disorders are associated with the cytoplasmic aggregation of microtubule-associated protein tau. Recent evidence supports transcellular transfer of tau misfolding (seeding) as the mechanism of spread within an affected brain, a process reminiscent of viral infection...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1607215114

    authors： McEwan WA,Falcon B,Vaysburd M,Clift D,Oblak AL,Ghetti B,Goedert M,James LC

    更新日期：2017-01-17 00:00:00

  • S-nitrosylation of proteins with nitric oxide: synthesis and characterization of biologically active compounds.

    abstract：:Endothelium-derived relaxing factor (EDRF) activity has been attributed to the highly labile nitric oxide radical (NO). In view of the fact that the plasma and cellular milieux contain reactive species that can rapidly inactivate NO, it has been postulated that NO is stabilized by a carrier molecule that preserves its...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.1.444

    authors： Stamler JS,Simon DI,Osborne JA,Mullins ME,Jaraki O,Michel T,Singel DJ,Loscalzo J

    更新日期：1992-01-01 00:00:00

  • Site-specific antibodies against the PrlA (secY) protein of Escherichia coli inhibit protein export by interfering with plasma membrane binding of preproteins.

    abstract：:Genetic evidence indicates that the PrlA (SecY) protein of Escherichia coli functions as a membrane integrated signal sequence receptor in protein "export"--i.e., in protein translocation across (or integration into) the plasma membrane. We have raised antibodies in rabbits against two synthetic peptides representing ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.6.1895

    authors： Watanabe M,Blobel G

    更新日期：1989-03-01 00:00:00

  • Catalytic asymmetric addition of diphenylzinc to cyclic alpha,beta-unsaturated ketones.

    abstract：:We have examined the use of our bis(sulfonamide) diol ligand (1) in the asymmetric addition of phenyl groups to cyclic alpha,beta-unsaturated ketones. Good to excellent enantioselectivities have been obtained with cyclic enones bearing alkyl substituents in the 2 position (71-97% enantiomeric excess). Furthermore, exc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0307119101

    authors： Li H,García C,Walsh PJ

    更新日期：2004-04-13 00:00:00

  • Construction of an extended three-dimensional idiotope map by electron microscopic analysis of idiotope-anti-idiotope complexes.

    abstract：:A three-dimensional map of the positions of four idiotypic determinants (idiotopes or Ids) and an isotypic determinant was derived by transmission electron microscopy of negatively stained immune complexes. Each complex was composed of a monoclonal Id-expressing IgG and one or two varieties of monoclonal anti-Id (or a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.14.4984

    authors： Roux KH,Monafo WJ,Davie JM,Greenspan NS

    更新日期：1987-07-01 00:00:00

  • Methylation of a membrane protein involved in bacterial chemotaxis.

    abstract：:A protein methylation reaction involved in chemotaxis of Escherichia coli has been identified. The involvement of this reaction in chemotaxis in indicated by four lines of evidence. (a) The methylation reaction is altered in several classes of generally nonchemotactic mutants and is coreverted with the chemotaxis defe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.10.3939

    authors： Kort EN,Goy MF,Larsen SH,Adler J

    更新日期：1975-10-01 00:00:00

  • Evidence on the impact of sustained exposure to air pollution on life expectancy from China's Huai River policy.

    abstract：:This paper's findings suggest that an arbitrary Chinese policy that greatly increases total suspended particulates (TSPs) air pollution is causing the 500 million residents of Northern China to lose more than 2.5 billion life years of life expectancy. The quasi-experimental empirical approach is based on China's Huai ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1300018110

    authors： Chen Y,Ebenstein A,Greenstone M,Li H

    更新日期：2013-08-06 00:00:00

  • Glucose sensing by MondoA:Mlx complexes: a role for hexokinases and direct regulation of thioredoxin-interacting protein expression.

    abstract：:Glucose is a fundamental metabolite, yet how cells sense and respond to changes in extracellular glucose concentration is not completely understood. We recently reported that the MondoA:Mlx dimeric transcription factor directly regulates glycolysis. In this article, we consider whether MondoA:Mlx complexes have a broa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0712199105

    authors： Stoltzman CA,Peterson CW,Breen KT,Muoio DM,Billin AN,Ayer DE

    更新日期：2008-05-13 00:00:00