Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein.

abstract：:Hereditary deficiency of factor IXa (fIXa), a key enzyme in blood coagulation, causes hemophilia B, a severe X chromosome-linked bleeding disorder afflicting 1 in 30,000 males; clinical studies have identified nearly 500 deleterious variants. The x-ray structure of porcine fIXa described here shows the atomic origins ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brandstetter H,Bauer M,Huber R,Lollar P,Bode W

更新日期：1995-10-10 00:00:00

abstract：:How genome divergence eventually leads to speciation is a topic of prime evolutionary interest. Genomic islands of elevated divergence are frequently reported between diverging lineages, and their size is expected to increase with time and gene flow under the speciation-with-gene-flow model. However, such islands can ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma T,Wang K,Hu Q,Xi Z,Wan D,Wang Q,Feng J,Jiang D,Ahani H,Abbott RJ,Lascoux M,Nevo E,Liu J

更新日期：2018-01-09 00:00:00

abstract：:Telomerase is an RNA-directed DNA polymerase, composed of RNA and protein subunits, that replicates the telomere ends of linear eukaryotic chromosomes. Using a genetic strategy described here, we identify the product of the EST2 gene, Est2p, as a subunit of telomerase in the yeast Saccharomyces cerevisiae. Est2p is re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Counter CM,Meyerson M,Eaton EN,Weinberg RA

更新日期：1997-08-19 00:00:00

abstract：:This paper describes a variety of statistical methods for obtaining precise quantitative estimates of the similarities and differences in the structures of semantic domains in different languages. The methods include comparing mean correlations within and between groups, principal components analysis of interspeaker c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Romney AK,Moore CC,Batchelder WH,Hsia TL

更新日期：2000-01-04 00:00:00

abstract：:The SOS-inducible ruvA and ruvB gene products of Escherichia coli are required for normal levels of genetic recombination and DNA repair. In vitro, RuvA protein interacts specifically with Holliday junctions and, together with RuvB (an ATPase), promotes their movement along DNA. This process, known as branch migration...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsaneva IR,Müller B,West SC

更新日期：1993-02-15 00:00:00

abstract：:This study uses a multiisotope (carbon, nitrogen, oxygen, and strontium) approach to examine early animal management in the Maya region. An analysis of faunal specimens across almost 2,000 years (1000 BC to AD 950) at the site of Ceibal, Guatemala, reveals the earliest evidence for live-traded dogs and possible captiv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharpe AE,Emery KF,Inomata T,Triadan D,Kamenov GD,Krigbaum J

更新日期：2018-04-03 00:00:00

abstract：:A well-known indication that a nucleotide has bound to myosin is the enhancement of the fluorescence of a specific tryptophan in the "subfragment 1" segment of the protein. Empirically the effect has been enormously useful in myosin enzymology. But beyond an early suggestion that it arises from a purine-tryptophan cha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bivin DB,Kubota S,Pearlstein R,Morales MF

更新日期：1993-07-15 00:00:00

abstract：:Genetic and biochemical studies on enzymes known to be involved in regulating DNA supercoiling yield a complex spectrum of effects on the Escherichia coli SOS system. Previous studies indicated that only inhibition of DNA gyrase by antibiotics that act on the DNA gyrase A subunit results in turning on the E. coli SOS ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith CL

更新日期：1983-05-01 00:00:00

abstract：:Although the future often seems intangible, we can make it more concrete by imagining prospective events. Here, using functional MRI, we demonstrate a mechanism by which the ventromedial prefrontal cortex supports such episodic simulations, and thereby contributes to affective foresight: This region supports processes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benoit RG,Szpunar KK,Schacter DL

更新日期：2014-11-18 00:00:00

abstract：:We have isolated, by molecular cloning and genetic complementation of a listeriolysin-negative mutant, a gene required for the expression of this virulence factor in Listeria monocytogenes. The mutant strain SLCC53, which was nonhemolytic and avirulent, harbored a deletion of 450 base pairs located approximately 1500 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leimeister-Wächter M,Haffner C,Domann E,Goebel W,Chakraborty T

更新日期：1990-11-01 00:00:00

abstract：:The assembly of heterogeneous nuclear RNA (hnRNA) into ribonucleoprotein (RNP) particles has been investigated during in vitro transcription in isolated nuclei. Approximately 80% of the in vitro transcription observed in mouse Friend erythroleukemia cell nuclei is attributable to the activity of RNA polymerase II. In ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Economidis IV,Pederson T

更新日期：1982-03-01 00:00:00

abstract：:Some of the most important impacts of global climate change will be felt among the populations, predominantly in developing countries, referred to as "subsistence" or "smallholder" farmers. Their vulnerability to climate change comes both from being predominantly located in the tropics, and from various socioeconomic,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morton JF

更新日期：2007-12-11 00:00:00

abstract：:Natural ecosystems contain many individuals and species interacting with each other and with their abiotic environment. Such systems can be expected to exhibit complex dynamics in which small perturbations can be amplified to cause large changes. Here, we document the reorganization of an arid ecosystem that has occur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown JH,Valone TJ,Curtin CG

更新日期：1997-09-02 00:00:00

abstract：:We report the synthesis of a probe that permits the visualization by electron microscopy of acidic organelles in intact cells. This probe, 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine (DAMP), is a basic congener of dinitrophenol that readily diffuses into intact cells. Its primary and tertiary amino groups (a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson RG,Falck JR,Goldstein JL,Brown MS

更新日期：1984-08-01 00:00:00

abstract：:Crithidia fasciculata cells were treated with a plasmid (pDK96) containing pBR322 sequences, a Leishmania tarentolae maxicircle autonomously replicating sequence, and the bacterial gene for aminoglycoside 3' phosphotransferase I inserted between the yeast alcohol dehydrogenase 1 promotor and terminator sequences. Resi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes DE,Simpson L

更新日期：1986-08-01 00:00:00

abstract：:The Arabidopsis thaliana AtHKT1 protein, a Na(+)/K(+) transporter, is capable of mediating inward Na(+) currents in Xenopus laevis oocytes and K(+) uptake in Escherichia coli. HKT1 proteins are members of a superfamily of K(+) transporters. These proteins have been proposed to contain eight transmembrane segments and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kato Y,Sakaguchi M,Mori Y,Saito K,Nakamura T,Bakker EP,Sato Y,Goshima S,Uozumi N

更新日期：2001-05-22 00:00:00

abstract：:The properties of the virus synthesized by each of three morphologically different cell lines originating from DBA/2J fetal liver cells transformed by the anemic strain of Friend leukemia virus in vitro were analyzed. The cells of line G-1 are malignant in syngeneic DBA/2 mice, grow in suspension, and are erythroid in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsuei D,Pogo BG,Friend C

更新日期：1980-10-01 00:00:00

abstract：:B-1a cells reside in both the peritoneal cavity and the spleen. LPS stimulates splenic B-1a to differentiate to plasma cells producing natural IgM specific for microbial and self antigens. However, there are conflicting views as to whether the B-1a cells divide before this differentiation occurs, and hence how the res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Y,Tung JW,Ghosn EE,Herzenberg LA,Herzenberg LA

更新日期：2007-03-13 00:00:00

abstract：:The anticancer peptide PNC-27, which contains an HDM-2-binding domain corresponding to residues 12-26 of p53 and a transmembrane-penetrating domain, has been found to kill cancer cells (but not normal cells) by inducing membranolysis. We find that our previously determined 3D structure of the p53 residues of PNC-27 is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sarafraz-Yazdi E,Bowne WB,Adler V,Sookraj KA,Wu V,Shteyler V,Patel H,Oxbury W,Brandt-Rauf P,Zenilman ME,Michl J,Pincus MR

更新日期：2010-02-02 00:00:00

abstract：:Studies of natural killer (NK) cell function in vivo have been challenging primarily due to the lack of animal models in which NK cells are genetically and selectively deficient. Here, we describe a transgenic mouse with defective natural killing and selective deficiency in NK1.1(+) CD3(-) cells. Despite functionally ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim S,Iizuka K,Aguila HL,Weissman IL,Yokoyama WM

更新日期：2000-03-14 00:00:00

abstract：:Serum from bacillus Calmette-Guerin-infected mice injected with endotoxin induces the appearance of surface immunoglobulin, Ia antigen, and complement receptor on the surface of precursor bone-marrow-derived (B) cells. While endotoxin itself causes phenotypic conversion of both thymus-derived (T) cells and B cells in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoffmann MK,Oettgen HF,Old LJ,Chin AF,Hammerling U

更新日期：1977-03-01 00:00:00

abstract：:Ventilator-induced diaphragmatic dysfunction (VIDD) refers to the diaphragm muscle weakness that occurs following prolonged controlled mechanical ventilation (MV). The presence of VIDD impedes recovery from respiratory failure. However, the pathophysiological mechanisms accounting for VIDD are still not fully understo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matecki S,Dridi H,Jung B,Saint N,Reiken SR,Scheuermann V,Mrozek S,Santulli G,Umanskaya A,Petrof BJ,Jaber S,Marks AR,Lacampagne A

更新日期：2016-08-09 00:00:00

abstract：:To understand the pathogenesis that may be induced by human adenovirus type 12 (Ad12), we have generated transgenic mice carrying the Ad12 early region 1 under control of the mouse mammary tumor virus long terminal repeat. Eleven of 11 male founder mice, but only 2 of 12 females, died between 3 to 4 mo of age. Death w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koike K,Hinrichs SH,Isselbacher KJ,Jay G

更新日期：1989-07-01 00:00:00

abstract：:Hydrocarbon films confined between smooth mica surfaces have long provided an experimental playground for model studies of structure and dynamics of confined liquids. However, fundamental questions regarding the phase behavior and shear properties in this simple system remain unsolved. With ultrasensitive resolution i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith AM,Hallett JE,Perkin S

更新日期：2019-12-17 00:00:00

abstract：:Five patients with advanced breast cancer were treated with pooled dialyzable transfer factor from healthy adult donors. The period of treatment ranged from 21 to 310 days, the total dose from 20 to 257 ml. Transfer factor did not elicit inflammatory or hypersensitivity reactions or detectable formation of antibody to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oettgen HF,Old LJ,Farrow JH,Valentine FT,Lawrence HS,Thomas L

更新日期：1974-06-01 00:00:00

abstract：:B-1 B cells have been proposed to be preferentially generated from fetal progenitors, but this view is challenged by studies concluding that B-1 production is sustained throughout adult life. To address this controversy, we compared the efficiency with which hematopoietic stem cells (HSCs) and common lymphoid progenit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barber CL,Montecino-Rodriguez E,Dorshkind K

更新日期：2011-08-16 00:00:00

abstract：:Selective harvest of large individuals should alter natural adaptive landscapes and drive evolution toward reduced somatic growth and increased reproductive investment. However, few studies have simultaneously considered the relative importance of artificial and natural selection in driving trait changes in wild popul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Edeline E,Carlson SM,Stige LC,Winfield IJ,Fletcher JM,James JB,Haugen TO,Vøllestad LA,Stenseth NC

更新日期：2007-10-02 00:00:00

abstract：:The complete coding region of maize transposable element Ac and truncated but active derivatives of it were placed under the control of promoters of different strength and tested for the ability to excise transposable element Ds from a beta-glucuronidase reporter gene in a cotransfection assay in Petunia protoplasts. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Becker D,Lütticke R,Li M,Starlinger P

更新日期：1992-06-15 00:00:00

abstract：:The distribution of oriented, polymerized sickle cell hemoglobin (hemoglobin S) in erythrocytes is visualized with a microscope that produces an image proportional to linear dichroism. Monochromatic light alternately polarized along two perpendicular directions is incident on the sample. The image is focused on a diod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mickols W,Maestre MF,Tinoco I Jr,Embury SH

更新日期：1985-10-01 00:00:00

abstract：:Contemporary climate change is characterized both by increasing mean temperature and increasing climate variability such as heat waves, storms, and floods. How populations and communities cope with such climatic extremes is a question central to contemporary ecology and biodiversity conservation. Previous work has sho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reusch TB,Ehlers A,Hämmerli A,Worm B

更新日期：2005-02-22 00:00:00