In vivo natural killer cell activities revealed by natural killer cell-deficient mice.

abstract：:Fluorescein isothiocyanate (FITC) was used to label the rabbit intestinal brush border Na+-glucose carrier, identify the carrier protein on sodium dodecyl sulfate/polyacrylamide gel electrophoresis, and monitor the effect of ions and substrates on fluorescence quenching. Enriched brush border preparations were employe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peerce BE,Wright EM

更新日期：1984-04-01 00:00:00

abstract：:The bacterial superantigen staphylococcal enterotoxin A (SEA) is an extremely potent activator of T lymphocytes when presented on major histocompatibility complex (MHC) class II molecules. To develop a tumor-specific superantigen for cancer therapy, we have made a recombinant fusion protein of SEA and the Fab region o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dohlsten M,Abrahmsén L,Björk P,Lando PA,Hedlund G,Forsberg G,Brodin T,Gascoigne NR,Förberg C,Lind P

更新日期：1994-09-13 00:00:00

abstract：:N-Acetylaspartylglutamate, an endogenous brain peptide that binds with high affinity to a subpopulation of glutamate-binding sites in rat brain, is excitatory on rat piriform cortex pyramidal cells studied in a perfused brain slice. Both the monosynaptic excitation of the pyramidal cells elicited by stimulation of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ffrench-Mullen JM,Koller K,Zaczek R,Coyle JT,Hori N,Carpenter DO

更新日期：1985-06-01 00:00:00

abstract：:Asian rice, Oryza sativa, is one of world's oldest and most important crop species. Rice is believed to have been domesticated ∼9,000 y ago, although debate on its origin remains contentious. A single-origin model suggests that two main subspecies of Asian rice, indica and japonica, were domesticated from the wild ric...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Molina J,Sikora M,Garud N,Flowers JM,Rubinstein S,Reynolds A,Huang P,Jackson S,Schaal BA,Bustamante CD,Boyko AR,Purugganan MD

更新日期：2011-05-17 00:00:00

abstract：:An 125I-labeled synthetic analog of bovine parathyroid hormone, [8-norleucine,18-norleucine,34-tyrosine]PTH-(1-34) amide ([Nle]PTH-(1-34)-NH2), purified by high-pressure liquid chromatography (HPLC), was employed to label the parathyroid hormone (PTH) receptor in cell lines derived from PTH target tissues: the ROS 17/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wright BS,Tyler GA,O'Brien R,Caporale LH,Rosenblatt M

更新日期：1987-01-01 00:00:00

abstract：:Trigger factor (TF), the first chaperone in eubacteria to encounter the emerging nascent chain, binds to the large ribosomal subunit in the vicinity of the protein exit tunnel opening and forms a sheltered folding space. Here, we present the 3.5-A crystal structure of the physiological complex of the large ribosomal s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baram D,Pyetan E,Sittner A,Auerbach-Nevo T,Bashan A,Yonath A

更新日期：2005-08-23 00:00:00

abstract：:Both treatment of disease and basic studies of complex tissues can benefit from directing viral vector infection to specific cell types. We have used a unique cell targeting method to direct viral vector transduction to cerebral cortical neurons expressing the neuregulin (NRG) receptor ErbB4; both NRG and ErbB4 have b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Choi J,Young JA,Callaway EM

更新日期：2010-09-21 00:00:00

abstract：:The homogeneous exchange process whereby IgG molecules adsorbed onto latex particles are replaced by IgG molecules from the bulk solution was studied by means of 125I radiolabeling. The exchange mechanism was investigated on surfaces saturated with either labeled or unlabeled proteins in the presence of a solution of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ball V,Huetz P,Elaissari A,Cazenave JP,Voegel JC,Schaaf P

更新日期：1994-07-19 00:00:00

abstract：:Our laboratory has previously developed cell lines derived from mouse NIH 3T3 fibroblasts and C127 mammary tumor cells that stably express mouse mammary tumor virus (MMTV) long terminal repeat fusion genes in bovine papillomavirus-based episomes. Glucocorticoid hormone strongly activates transcription from episomes an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bresnick EH,John S,Berard DS,LeFebvre P,Hager GL

更新日期：1990-05-01 00:00:00

abstract：:The transcription factor Krüppel-like factor 2 (KLF2) is critical for normal trafficking of T lymphocytes, but its role in B cells is unclear. We report that B cell-specific KLF2 deficiency leads to decreased expression of the trafficking molecules CD62L and β7-integrin, yet expression of sphingosine-1 phosphate recep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hart GT,Wang X,Hogquist KA,Jameson SC

更新日期：2011-01-11 00:00:00

abstract：:These studies sought to determine if neurons in the estrogen receptor-alpha knockout (ERalphaKO) mouse brain concentrated 16alpha-[125I]iodo-11beta-methoxy-17beta-estradiol (125I-estrogen), and if so, whether estrogen binding augmented the expression of progesterone receptor (PR) mRNA. Mice were injected with 125I-est...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shughrue PJ,Lubahn DB,Negro-Vilar A,Korach KS,Merchenthaler I

更新日期：1997-09-30 00:00:00

abstract：:In early stages of various pulmonary diseases, such as emphysema and fibrosis, the change in X-ray attenuation is not detectable with absorption-based radiography. To monitor the morphological changes that the alveoli network undergoes in the progression of these diseases, we propose using the dark-field signal, which...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schleede S,Meinel FG,Bech M,Herzen J,Achterhold K,Potdevin G,Malecki A,Adam-Neumair S,Thieme SF,Bamberg F,Nikolaou K,Bohla A,Yildirim AÖ,Loewen R,Gifford M,Ruth R,Eickelberg O,Reiser M,Pfeiffer F

更新日期：2012-10-30 00:00:00

abstract：:Despite their importance for biological activity, slower molecular motions beyond the nanosecond range remain poorly understood. We have assembled an unprecedented set of experimental NMR data, comprising up to 27 residual dipolar couplings per amino acid, to define the nature and amplitude of backbone motion in prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bouvignies G,Bernadó P,Meier S,Cho K,Grzesiek S,Brüschweiler R,Blackledge M

更新日期：2005-09-27 00:00:00

abstract：:After uptake by the endosome of an antigen-presenting cell (APC), exogenous proteins are known to be degraded into peptides by protease digestion. Here, we report the mechanism by which pure carbohydrates can be depolymerized within APC endosomes/lysosomes by nitric oxide (NO)-derived reactive nitrogen species (RNSs) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duan J,Avci FY,Kasper DL

更新日期：2008-04-01 00:00:00

abstract：:We have investigated whether interleukin 3 (IL-3) supports the growth of cells of different lineages by the same mechanism(s). The experiments were carried out with Ea3 cells, a mouse pre-B cell line, and S-480-3 cells, a mouse basophil cell line, both of which are totally IL-3 dependent. We found that Ea3 lymphocytes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Palacios R,Garland J

更新日期：1984-02-01 00:00:00

abstract：:Whereas the laws of thermodynamics prohibit extraction of useful work from the Brownian motion of particles in equilibrium, these motions can be "rectified" under nonequilibrium conditions, for example, in the presence of asymmetric geometrical obstacles. Here, we describe a class of systems in which aerobic bacteria ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sokolov A,Apodaca MM,Grzybowski BA,Aranson IS

更新日期：2010-01-19 00:00:00

abstract：:A mutant factor IX, designated factor IXCambridge, was isolated from a patient with hemophilia B. This protein includes an 18-residue propeptide attached to the NH2 terminus of factor IX. A point mutation at residue -1, from an arginine to a serine, precludes cleavage of the propeptide by a processing protease and int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Diuguid DL,Rabiet MJ,Furie BC,Liebman HA,Furie B

更新日期：1986-08-01 00:00:00

abstract：:Polarization of optical fields is a crucial degree of freedom in the all-optical analogue of electromagnetically induced transparency (EIT). However, the physical origins of EIT and polarization-induced phenomena have not been well distinguished, which can lead to confusion in associated applications such as slow ligh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang C,Jiang X,Sweeney WR,Hsu CW,Liu Y,Zhao G,Peng B,Zhang M,Jiang L,Stone AD,Yang L

更新日期：2021-01-19 00:00:00

abstract：:FRET between the zinc porphyrin (ZnP) chromophore in zinc-substituted cytochrome c (Zn-cyt c) and an Alexa Fluor dye attached to specific surface sites was used to characterize Zn-cyt c unfolding. The use of ZnP as a fluorescent acceptor eliminates the need to doubly label the protein with exogenous dyes to perform FR...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ensign AA,Jo I,Yildirim I,Krauss TD,Bren KL

更新日期：2008-08-05 00:00:00

abstract：:A modified differential screening procedure was applied to analyze cDNA libraries of cloned helper T lymphocytes (Th) and cytolytic T lymphocytes (CTL). Negative and positive differential screening and RNA blot analysis were used to identify cDNA clones that were expressed preferentially in Th or CTL. Seven clones cor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kwon BS,Kim GS,Prystowsky MB,Lancki DW,Sabath DE,Pan JL,Weissman SM

更新日期：1987-05-01 00:00:00

abstract：:General optimization results from physics indicate that maximum efficiency of a process, in the sense of minimum overall entropy production, is achieved when the rate of entropy production is constant over time, however not in ordinary clock time but on an, in general varying, "eigen time" scale, intrinsic to the syst...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Andresen B,Shiner JS,Uehlinger DE

更新日期：2002-04-30 00:00:00

abstract：:The all-cis hexose D-allose, fed to hamster fibroblast cultures over 14-20 hr, brings about a striking down-regulation of hexose transport. This down-regulation by allose is inhibited by 2,4-dinitrophenol. By using D-[14C]allose and thin-layer chromatography, two types of products accumulated that have been identified...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ullrey DB,Kalckar HM

更新日期：1991-02-15 00:00:00

abstract：:Upon stimulation with anti-CD3, suppressor T-cell (Ts) hybridomas and homologous transfectants of T-cell receptor a (TCRalpha) cDNA in the T-cell hybridoma formed a 55-kDa TCRalpha chain derivative that bound both the monoclonal anti-TCRalpha chain and polyclonal antibodies against glycosylation inhibiting factor (GIF...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishii Y,Nakano T,Ishizaka K

更新日期：1996-07-09 00:00:00

abstract：:We have established a large panel of T-cell clones (TCCs) specific for Dermatophagoides pteronyssinus and Lolium perenne group I grass pollen allergens (total, 61) and for tetanus toxoid and protein purified derivative bacterial antigens (total, 38) from the peripheral blood of two atopic individuals and then analyzed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parronchi P,Macchia D,Piccinni MP,Biswas P,Simonelli C,Maggi E,Ricci M,Ansari AA,Romagnani S

更新日期：1991-05-15 00:00:00

abstract：:Two-dimensional gel electrophoresis of immunoprecipitated human HLA-DR antigens from cells expressing the HLA-DR4 haplotype shows distinct clustering of beta-chain patterns. Six unique electrophoretic variants were observed among 17 HLA-DR4 homozygous cell lines (HCL) analyzed. These patterns correlate precisely with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nepom BS,Nepom GT,Mickelson E,Antonelli P,Hansen JA

更新日期：1983-11-01 00:00:00

abstract：:Voltage-dependent anion channels (VDACs) are pore-forming proteins (porins) that form the major pathway for movement of adenine nucleotides through the outer mitochondrial membrane. Electrophysiological studies indicate that VDAC-like channel activity is also prevalent in the cell membranes of many mammalian cells. Ho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Buettner R,Papoutsoglou G,Scemes E,Spray DC,Dermietzel R

更新日期：2000-03-28 00:00:00

abstract：:Cerebral organization during sentence processing in English and in American Sign Language (ASL) was characterized by employing functional magnetic resonance imaging (fMRI) at 4 T. Effects of deafness, age of language acquisition, and bilingualism were assessed by comparing results from (i) normally hearing, monolingua...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neville HJ,Bavelier D,Corina D,Rauschecker J,Karni A,Lalwani A,Braun A,Clark V,Jezzard P,Turner R

更新日期：1998-02-03 00:00:00

abstract：:One approach to understanding common human diseases is to determine the genetic defects responsible for similar diseases in animal models and place those defective genes in their corresponding biochemical pathways. Our laboratory is working with an animal model for human rheumatoid arthritis called collagen-induced ar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McIndoe RA,Bohlman B,Chi E,Schuster E,Lindhardt M,Hood L

更新日期：1999-03-02 00:00:00

abstract：:Hematologic malignancies are frequently associated with cardiac pathologies. Mutations of isocitrate dehydrogenase 1 and 2 (IDH1/2) occur in a subset of acute myeloid leukemia patients, causing metabolic and epigenetic derangements. We have now discovered that altered metabolism in leukemic cells has a profound effect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karlstaedt A,Zhang X,Vitrac H,Harmancey R,Vasquez H,Wang JH,Goodell MA,Taegtmeyer H

更新日期：2016-09-13 00:00:00

abstract：:In Salmonella typhimurium, the CobU, CobS, CobT, and CobC proteins have been proposed to catalyze the late steps in adenosylcobalamin biosynthesis, which define the nucleotide loop assembly pathway. This paper reports the in vitro assembly of the nucleotide loop of adenosylcobalamin from its precursors adenosylcobinam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maggio-Hall LA,Escalante-Semerena JC

更新日期：1999-10-12 00:00:00