Abstract:
:The immunocytochemical demonstration of (pro)insulin on intracellular membrane compartments of the pancreatic B cell reveals that the immunolabeling detected by the protein A/gold method is associated, at the level of the Golgi apparatus, with the inner aspect of the cisternal membranes; on the secretory granules, by contrast, insulin immunoreactive sites predominate over the granule core, and very little immunoreactivity is associated with the granule membrane. The localization of (pro)insulin immunoreactivity on Golgi membranes is compatible with the presence, at this level, of specific binding sites for (pro)insulin, which could be related to the proteolytic processing or sorting out (or both) of these peptides on their way from the rough endoplasmic reticulum to the storage secretory granules.
journal_name
Proc Natl Acad Sci U S Aauthors
Orci L,Ravazzola M,Perrelet Adoi
10.1073/pnas.81.21.6743subject
Has Abstractpub_date
1984-11-01 00:00:00pages
6743-6issue
21eissn
0027-8424issn
1091-6490journal_volume
81pub_type
杂志文章abstract::The C-C chemokine receptor 5 (CCR5) plays a crucial role in facilitating the entry of macrophage-tropic strains of the HIV-1 into cells, but the mechanism of this phenomenon is completely unknown. To explore the role of CCR5-derived signal transduction in viral entry, we introduced mutations into two cytoplasmic domai...
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