Abstract:
:Several yeast activators are phosphorylated by SRB10, a cyclin-dependent kinase associated with the transcriptional machinery. Sites of phosphorylation are found outside the activating region in each case, and the modification has different physiological consequences in different cases. We show here that certain acidic transcriptional activating regions contact SRB10 as assayed both in vivo and in vitro. The interaction evidently positions each activator, as it activates transcription, so that it gets phosphorylated by SRB10, and thus a common mechanism targets disparate substrates to the kinase.
journal_name
Proc Natl Acad Sci U S Aauthors
Ansari AZ,Koh SS,Zaman Z,Bongards C,Lehming N,Young RA,Ptashne Mdoi
10.1073/pnas.232573899keywords:
subject
Has Abstractpub_date
2002-11-12 00:00:00pages
14706-9issue
23eissn
0027-8424issn
1091-6490pii
232573899journal_volume
99pub_type
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