Vacuolar H(+)-pumping ATPase variable transport coupling ratio controlled by pH.

abstract：:Band 3, the major intrinsic protein of the human erythrocyte membrane, was specifically labeled with the covalent fluorescent probe eosin isothiocyanate. The lateral mobility of labeled band 3 in the plane of the membrane under various conditions of ionic strength and temperature was examined by using the fluorescence...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Golan DE,Veatch W

更新日期：1980-05-01 00:00:00

abstract：:While the concept of intercellular mechanical communication has been revealed, the mechanistic insights have been poorly evidenced in the context of myofibroblast-fibroblast interaction during fibrosis expansion. Here we report and systematically investigate the mechanical force-mediated myofibroblast-fibroblast cross...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu L,Yu H,Zhao H,Wu Z,Long Y,Zhang J,Yan X,You Z,Zhou L,Xia T,Shi Y,Xiao B,Wang Y,Huang C,Du Y

更新日期：2020-05-19 00:00:00

abstract：:Human tuberculosis is caused by members of the Mycobacterium tuberculosis complex (MTBC) that vary in virulence and transmissibility. While genome-wide association studies have uncovered several mutations conferring drug resistance, much less is known about the factors underlying other bacterial phenotypes. Variation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Øyås O,Borrell S,Trauner A,Zimmermann M,Feldmann J,Liphardt T,Gagneux S,Stelling J,Sauer U,Zampieri M

更新日期：2020-04-14 00:00:00

abstract：:Cisplatin is a chemotherapeutic drug used to treat a variety of cancers. Both intrinsic and acquired resistance to cisplatin, as well as toxicity, limit its effectiveness. Molecular mechanisms that underlie cisplatin resistance are poorly understood. Here we demonstrate that deletion of the yeast CTR1 gene, which enco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishida S,Lee J,Thiele DJ,Herskowitz I

更新日期：2002-10-29 00:00:00

abstract：:Impaired magnesium reabsorption in patients with TRPM6 gene mutations stresses an important role of TRPM6 (melastatin-related TRP cation channel) in epithelial magnesium transport. While attempting to isolate full-length TRPM6, we found that the human TRPM6 gene encodes multiple mRNA isoforms. Full-length TRPM6 varian...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chubanov V,Waldegger S,Mederos y Schnitzler M,Vitzthum H,Sassen MC,Seyberth HW,Konrad M,Gudermann T

更新日期：2004-03-02 00:00:00

abstract：:Expression of glucokinase in hepatocytes and pancreatic 6-cells is of major physiologic importance to mammalian glucose homeostasis. Liver glucokinase catalyzes the first committed step in the disposal of glucose, and beta-cell glucokinase catalyzes a rate-limiting step required for glucose-regulated insulin release. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heimberg H,De Vos A,Moens K,Quartier E,Bouwens L,Pipeleers D,Van Schaftingen E,Madsen O,Schuit F

更新日期：1996-07-09 00:00:00

abstract：:Pathway proteomics strategies measure protein expression changes in specific cellular processes that carry out related functions. Using targeted tandem mass tags-based sample multiplexing, hundreds of proteins can be quantified across 10 or more samples simultaneously. To facilitate these highly complex experiments, w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu Q,Xiao H,Jedrychowski MP,Schweppe DK,Navarrete-Perea J,Knott J,Rogers J,Chouchani ET,Gygi SP

更新日期：2020-05-05 00:00:00

abstract：:This paper shows that the most predictable components of internal variability in coupled atmosphere-ocean models are remarkably similar to the most predictable components of climate models without interactive ocean dynamics (i.e., models whose ocean is represented by a 50-m-deep slab ocean mixed layer with no interact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Srivastava A,DelSole T

更新日期：2017-02-28 00:00:00

abstract：:Peptide ligand selection by MHC class I molecules, which occurs by iterative optimization, is the centerpiece of immunodominance in antiviral and antitumor immune responses. For its understanding, the molecular mechanisms of peptide binding and dissociation by class I molecules must be elucidated. To this end, we have...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saini SK,Schuster H,Ramnarayan VR,Rammensee HG,Stevanović S,Springer S

更新日期：2015-01-06 00:00:00

abstract：:The formation of complex but highly organized neural circuits requires interactions between neurons and glia. During the assembly of the Drosophila olfactory circuit, 50 olfactory receptor neuron (ORN) classes and 50 projection neuron (PN) classes form synaptic connections in 50 glomerular compartments in the antennal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu B,Li J,Chou YH,Luginbuhl D,Luo L

更新日期：2017-07-18 00:00:00

abstract：:Transfer of organelle DNA into the nuclear genome has been significant in eukaryotic evolution, because it appears to be the origin of many nuclear genes. Most studies on organelle DNA transfer have been restricted to evolutionary events but experimental systems recently became available to monitor the process in real...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang CY,Ayliffe MA,Timmis JN

更新日期：2004-06-29 00:00:00

abstract：:The intracellular activation of trypsinogen, which is both pH- and calcium-dependent, is an important early step in the development of acute pancreatitis. The cellular compartment in which trypsinogen activation occurs currently is unknown. We therefore investigated the site of intracellular trypsinogen activation by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sherwood MW,Prior IA,Voronina SG,Barrow SL,Woodsmith JD,Gerasimenko OV,Petersen OH,Tepikin AV

更新日期：2007-03-27 00:00:00

abstract：:The redox siblings nitroxyl (HNO) and nitric oxide (NO) have often been assumed to undergo casual redox reactions in biological systems. However, several recent studies have demonstrated distinct pharmacological effects for donors of these two species. Here, infusion of the HNO donor Angeli's salt into normal dogs res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miranda KM,Paolocci N,Katori T,Thomas DD,Ford E,Bartberger MD,Espey MG,Kass DA,Feelisch M,Fukuto JM,Wink DA

更新日期：2003-08-05 00:00:00

abstract：:Gene regulation by imposed localization was studied by using designed zinc finger proteins that bind 18-bp DNA sequences in the 5' untranslated regions of the protooncogenes erbB-2 and erbB-3. Transcription factors were generated by fusion of the DNA-binding proteins to repression or activation domains. When introduce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beerli RR,Dreier B,Barbas CF 3rd

更新日期：2000-02-15 00:00:00

abstract：:Pro-Leu-Gly-NH2 (MIF) and several structural analogues, all injected in 50-microgram doses daily in mice receiving morphine chronically, were found to prevent development of physical dependence as measured by changes in body temperature associated with naloxone-induced withdrawal. Dose-response studies, using again a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walter R,Ritzmann RF,Bhargava HN,Flexner LB

更新日期：1979-01-01 00:00:00

abstract：:Serological expression cloning of antigens eliciting a humoral immune response to a syngeneic mouse sarcoma identified pem (mouse placenta and embryonic expression gene) as a new member of the cancer/testis family. To identify the human homologue of pem, mouse pem sequences and pem-related expressed sequence tags from...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ono T,Kurashige T,Harada N,Noguchi Y,Saika T,Niikawa N,Aoe M,Nakamura S,Higashi T,Hiraki A,Wada H,Kumon H,Old LJ,Nakayama E

更新日期：2001-03-13 00:00:00

abstract：:The assembly of the three neuronal soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins synaptobrevin 2, syntaxin-1A, and SNAP-25 is the key step that leads to exocytotic fusion of synaptic vesicles. In the fully assembled SNARE complex, these three proteins form a coiled-coil ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang B,Kiessling V,Tamm LK

更新日期：2013-11-26 00:00:00

abstract：:Understanding the heritability of neural systems linked to psychopathology is not sufficient to implicate them as intergenerational neural mediators. By closely examining how individual differences in neural phenotypes and psychopathology cosegregate as they fall through the family tree, we can identify the brain syst...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fox AS,Oler JA,Shackman AJ,Shelton SE,Raveendran M,McKay DR,Converse AK,Alexander A,Davidson RJ,Blangero J,Rogers J,Kalin NH

更新日期：2015-07-21 00:00:00

abstract：:Loss of a whole chromosome 5 or a deletion of its long arm (5q) is a recurring abnormality in malignant myeloid neoplasms. To determine the location of genes on 5q that may be involved in leukemogenesis, we examined the deleted chromosome 5 homologs in a series of 135 patients with malignant myeloid diseases. By compa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le Beau MM,Espinosa R 3rd,Neuman WL,Stock W,Roulston D,Larson RA,Keinanen M,Westbrook CA

更新日期：1993-06-15 00:00:00

abstract：:In 1950, John Nash contributed a remarkable one-page PNAS article that defined and characterized a notion of equilibrium for n- person games. This notion, now called the "Nash equilibrium," has been widely applied and adapted in economics and other behavioral sciences. Indeed, game theory, with the Nash equilibrium as...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holt CA,Roth AE

更新日期：2004-03-23 00:00:00

abstract：:How much free energy is irreversibly lost during a thermodynamic process? For deterministic protocols, lower bounds on energy dissipation arise from the thermodynamic friction associated with pushing a system out of equilibrium in finite time. Recent work has also bounded the cost of precisely moving a single degree o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bryant SJ,Machta BB

更新日期：2020-02-18 00:00:00

abstract：:Duplication of the genome during the S phase of the cell cycle does not occur simultaneously; rather, different sequences are replicated at different times. The replication timing of specific sequences can change during development; however, the determinants of this dynamic process are poorly understood. To gain insig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： White EJ,Emanuelsson O,Scalzo D,Royce T,Kosak S,Oakeley EJ,Weissman S,Gerstein M,Groudine M,Snyder M,Schübeler D

更新日期：2004-12-21 00:00:00

abstract：:Glial cells are traditionally regarded as elements for structural support and ionic homeostasis, but have recently attracted attention as putative integral elements of the machinery involved in synaptic transmission and plasticity. Here, we demonstrate that calcium-binding protein S100B, which is synthesized in consid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishiyama H,Knopfel T,Endo S,Itohara S

更新日期：2002-03-19 00:00:00

abstract：:H2O2, in concentrations achieved in the proximity of stimulated leukocytes, induces injury and lysis of target cells. This may be an important aspect of inflammatory injury of tissues. Cell lysis in two target cells, the murine macrophage-like tumor cell line P388D1 and human peripheral lymphocytes, was found to be as...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schraufstatter IU,Hyslop PA,Hinshaw DB,Spragg RG,Sklar LA,Cochrane CG

更新日期：1986-07-01 00:00:00

abstract：:Bves is an integral membrane protein with no determined function and no homology to proteins outside of the Popdc family. It is widely expressed throughout development in myriad organisms. Here, we demonstrate an interaction between Bves and guanine nucleotide exchange factor T (GEFT), a GEF for Rho-family GTPases. Th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith TK,Hager HA,Francis R,Kilkenny DM,Lo CW,Bader DM

更新日期：2008-06-17 00:00:00

abstract：:Capturing carbon dioxide from the atmosphere ("air capture") in an industrial process has been proposed as an option for stabilizing global CO(2) concentrations. Published analyses suggest these air capture systems may cost a few hundred dollars per tonne of CO(2), making it cost competitive with mainstream CO(2) miti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： House KZ,Baclig AC,Ranjan M,van Nierop EA,Wilcox J,Herzog HJ

更新日期：2011-12-20 00:00:00

abstract：:For study of the basis of an X-linked form of gout in man, several clonal lines deficient in hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) were selected from the human lymphoblast line WI-L2 by spontaneous and mutagen-induced resistance to 10 muM 8-azaguanine. Three groups could be defined: (1) clones wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lever JE,Nuki G,Seegmiller JE

更新日期：1974-07-01 00:00:00

abstract：:The difficulty of achieving robust functional expression of insect nicotinic acetylcholine receptors (nAChRs) has hampered our understanding of these important molecular targets of globally deployed neonicotinoid insecticides at a time when concerns have grown regarding the toxicity of this chemotype to insect pollina...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ihara M,Furutani S,Shigetou S,Shimada S,Niki K,Komori Y,Kamiya M,Koizumi W,Magara L,Hikida M,Noguchi A,Okuhara D,Yoshinari Y,Kondo S,Tanimoto H,Niwa R,Sattelle DB,Matsuda K

更新日期：2020-07-14 00:00:00

abstract：:A number of rare copy number variants (CNVs), including both deletions and duplications, have been associated with developmental disorders, including schizophrenia, autism, intellectual disability, and epilepsy. Pathogenicity may derive from dosage sensitivity of one or more genes contained within the CNV locus. To un...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McLysaght A,Makino T,Grayton HM,Tropeano M,Mitchell KJ,Vassos E,Collier DA

更新日期：2014-01-07 00:00:00

abstract：:S3I-201 (NSC 74859) is a chemical probe inhibitor of Stat3 activity, which was identified from the National Cancer Institute chemical libraries by using structure-based virtual screening with a computer model of the Stat3 SH2 domain bound to its Stat3 phosphotyrosine peptide derived from the x-ray crystal structure of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Siddiquee K,Zhang S,Guida WC,Blaskovich MA,Greedy B,Lawrence HR,Yip ML,Jove R,McLaughlin MM,Lawrence NJ,Sebti SM,Turkson J

更新日期：2007-05-01 00:00:00