Abstract:
:Bovine proinsulin has been crystallized under a variety of conditions at both neutral and acid pH. Microtechniques were employed with sample weights of about 200 mug and volumes of 5-20 mul. The crystalline preparations all differ from each other morphologically.X-ray photographs of one form, tetragonal bipyramids grown at pH 3 with added ammonium sulphate solution, established the space group P4(1)2(1)2 (or its enantiomorph P4(3)2(1)2). The cell dimensions are a = 50.8 +/- 0.2 A, c = 148.0 +/- 0.4 A. The asymmetric unit in this form is a dimer of proinsulin which is also the dominant species in solution at this pH.
journal_name
Proc Natl Acad Sci U S Aauthors
Fullerton WW,Potter R,Low BWdoi
10.1073/pnas.66.4.1213subject
Has Abstractpub_date
1970-08-01 00:00:00pages
1213-9issue
4eissn
0027-8424issn
1091-6490journal_volume
66pub_type
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