Regulation of mitochondrial protein synthesis by cytoplasmic proteins.

abstract：:Regulatory T cells (Treg) comprise multiple subsets and are important in controlling immunity and inflammation. However, the induction and mode of action of the various distinct Treg subsets remain ill defined, particularly in humans. Here, we describe a human CD8+ lymphocyte activation gene-3 (LAG-3)+CD25+FoxP3+ Treg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Joosten SA,van Meijgaarden KE,Savage ND,de Boer T,Triebel F,van der Wal A,de Heer E,Klein MR,Geluk A,Ottenhoff TH

更新日期：2007-05-08 00:00:00

abstract：:Gene expression networks are complicated by the assortment of regulatory factors that bind DNA and modulate transcription combinatorially. Single-cell measurements can reveal biological mechanisms hidden by population averages, but their value has not been fully explored in the context of mRNA regulation. Here, we ada...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang L,Brugge JS,Janes KA

更新日期：2011-10-04 00:00:00

abstract：:We show that a drop of liquid a few hundred microns in diameter placed under a solid, elastic, thin film (∼10 μm thick) causes it to bulge by tens of microns. The deformed shape is governed by equilibrium of tensions exerted by the various interfaces and the solid film, a form of Neumann's triangle. Unlike Young's equ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nadermann N,Hui CY,Jagota A

更新日期：2013-06-25 00:00:00

abstract：:Antimicrobial peptides constitute an important component of the mammalian innate immune response. Several types of antimicrobial peptides, including the beta-defensins, are produced at epithelial surfaces in response to infectious threats. Here we show that a class of small molecules, including l-isoleucine and severa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fehlbaum P,Rao M,Zasloff M,Anderson GM

更新日期：2000-11-07 00:00:00

abstract：:The cerebral cortex of mammals differentiates into functionally distinct areas that exhibit unique cytoarchitecture, connectivity, and molecular characteristics. Molecular specification of cells fated for limbic cortical areas, based on the expression of the limbic system-associated membrane protein (LAMP), occurs dur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barbe MF,Levitt P

更新日期：1992-05-01 00:00:00

abstract：:Carcinosarcomas (CSs) of the uterus and ovary are highly aggressive neoplasms containing both carcinomatous and sarcomatous elements. We analyzed the mutational landscape of 68 uterine and ovarian CSs by whole-exome sequencing. We also performed multiregion whole-exome sequencing comprising two carcinoma and sarcoma s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao S,Bellone S,Lopez S,Thakral D,Schwab C,English DP,Black J,Cocco E,Choi J,Zammataro L,Predolini F,Bonazzoli E,Bi M,Buza N,Hui P,Wong S,Abu-Khalaf M,Ravaggi A,Bignotti E,Bandiera E,Romani C,Todeschini P,Tas

更新日期：2016-10-25 00:00:00

abstract：:Humans are consciously aware of some memories and can make verbal reports about these memories. Other memories cannot be brought to consciousness, even though they influence behavior. This conspicuous difference in access to memories is central in taxonomies of human memory systems but has been difficult to document i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hampton RR

更新日期：2001-04-24 00:00:00

abstract：:Five different selectively deuterated analogs of staphylococcal nuclease (Foggi) were prepared having all but a selected few hydrogen atoms in the enzyme molecule replaced by deuterium. The (1)H-NMR spectra of these analogs are extremely simplified, since they consist of lines arising only from the remaining protonate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Putter I,Barreto A,Markley JL,Jardetzky O

更新日期：1969-12-01 00:00:00

abstract：:The human tumor necrosis factor-alpha (TNF-alpha) gene encodes a pleiotropic cytokine that plays a critical role in basic immunologic processes. To investigate the TNF-alpha regulatory region in the primate lineage, we isolated TNF-alpha promoters from representative great apes, Old World monkeys, and New World monkey...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leung JY,McKenzie FE,Uglialoro AM,Flores-Villanueva PO,Sorkin BC,Yunis EJ,Hartl DL,Goldfeld AE

更新日期：2000-06-06 00:00:00

abstract：:Our understanding of the molecular control of many disease pathologies requires the identification of direct substrates targeted by specific protein kinases. Here we describe an integrated proteomic strategy, termed kinase assay linked with phosphoproteomics, which combines a sensitive kinase reaction with endogenous ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xue L,Wang WH,Iliuk A,Hu L,Galan JA,Yu S,Hans M,Geahlen RL,Tao WA

更新日期：2012-04-10 00:00:00

abstract：:hK7 or human stratum corneum chymotryptic enzyme belongs to the human tissue kallikrein (hKs) serine proteinase family and is strongly expressed in the upper layers of the epidermis. It participates in skin desquamation but is also implicated in diverse skin diseases and is a potential biomarker of ovarian cancer. We ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Debela M,Hess P,Magdolen V,Schechter NM,Steiner T,Huber R,Bode W,Goettig P

更新日期：2007-10-09 00:00:00

abstract：:Antibodies specific for allelic determinants of the cell membrane alloantigen Ly-17 were found to react with genetically determined polymorphic sites on the murine IgG Fc receptor (Fc gamma R). Monoclonal antibodies specific for Ly-17.2 and the Fc gamma R detected identical populations of cells in thymus, spleen, lymp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holmes KL,Palfree RG,Hammerling U,Morse HC 3rd

更新日期：1985-11-01 00:00:00

abstract：:mAbs against tumor-associated carbohydrate antigens have the potential to play a prominent role in cancer immunotherapy. However, it has not been possible to fully exploit the clinical utility of such antibodies primarily, because those of adequate affinity could be derived only from murine sources. To address this pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mao S,Gao C,Lo CH,Wirsching P,Wong CH,Janda KD

更新日期：1999-06-08 00:00:00

abstract：:RNA-protein interactions underlie a wide range of cellular processes. Improved methods are needed to systematically map RNA-protein interactions in living cells in an unbiased manner. We used two approaches to target the engineered peroxidase APEX2 to specific cellular RNAs for RNA-centered proximity biotinylation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han S,Zhao BS,Myers SA,Carr SA,He C,Ting AY

更新日期：2020-09-08 00:00:00

abstract：:The neurofibromatosis type 1 (NF1) gene responsible for von Recklinghausen neurofibromatosis is related to regulators of ras proteins, and a portion of NF1 that is homologous to the ras GTPase-activating protein (GAP) encodes a similar GTPase-stimulating activity. We have raised rabbit antisera to a bacterially synthe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeClue JE,Cohen BD,Lowy DR

更新日期：1991-11-15 00:00:00

abstract：:Urinary acidification by the turtle bladder is due to a H+-ATPase that is located in the luminal membrane. The rate of H+ transport is stimulated by an increase in the ambient CO2. Using the fluorescent dye acridine orange, we showed that the mitochondria-rich cell of this equilibrium contains vesicles whose internal ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gluck S,Cannon C,Al-Awqati Q

更新日期：1982-07-01 00:00:00

abstract：:The eyes of some aquatic animals form images through reflective optics. Shrimp, lobsters, crayfish, and prawns possess reflecting superposition compound eyes, composed of thousands of square-faceted eye units (ommatidia). Mirrors in the upper part of the eye (the distal mirror) reflect light collected from many ommati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Palmer BA,Hirsch A,Brumfeld V,Aflalo ED,Pinkas I,Sagi A,Rosenne S,Oron D,Leiserowitz L,Kronik L,Weiner S,Addadi L

更新日期：2018-03-06 00:00:00

abstract：:A plasmid containing the rat preproinsulin I gene was entrapped in large liposomes and intravenously administered to rats. Four hours after inoculation, the livers were processed for the isolation of hepatocytes. Kupffer cells, and endothelial cells, DNA was purified, and the exogenous DNA was detected in the differen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soriano P,Dijkstra J,Legrand A,Spanjer H,Londos-Gagliardi D,Roerdink F,Scherphof G,Nicolau C

更新日期：1983-12-01 00:00:00

abstract：:Thiol-dependent redox regulation allows the rapid adaptation of chloroplast function to unpredictable changes in light intensity. Traditionally, it has been considered that chloroplast redox regulation relies on photosynthetically reduced ferredoxin (Fd), thioredoxins (Trxs), and an Fd-dependent Trx reductase (FTR), t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pérez-Ruiz JM,Naranjo B,Ojeda V,Guinea M,Cejudo FJ

更新日期：2017-11-07 00:00:00

abstract：:Somatic cell hybridization of mouse erythroleukemia (MEL) cells and HEL cells, a human erythroleukemia line that produces fetal (gamma) but fails to express adult (beta) globin, was used to test whether the expression of the two human globin genes is regulated cis or trans. An experimental approach using anti-human gl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Papayannopoulou TH,Lindsley D,Kurachi S,Lewison K,Hemenway T,Melis M,Anagnou NP,Najfeld V

更新日期：1985-02-01 00:00:00

abstract：:Excitation-evoked Ca(2+) influx is the fastest and most ubiquitous chemical trigger for cellular processes, including neurotransmitter release, muscle contraction, and gene expression. The voltage dependence and timing of Ca(2+) entry are thought to be functions of voltage-gated calcium (CaV) channels composed of a ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pantazis A,Savalli N,Sigg D,Neely A,Olcese R

更新日期：2014-12-23 00:00:00

abstract：:Facioscapulohumeral muscular dystrophy (FSHD), characterized by progressive muscle weakness and deterioration, is genetically linked to aberrant expression of DUX4 in muscle. DUX4, in its full-length form, is cytotoxic in nongermline tissues. Here, we designed locked nucleic acid (LNA) gapmer antisense oligonucleotide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lim KRQ,Maruyama R,Echigoya Y,Nguyen Q,Zhang A,Khawaja H,Sen Chandra S,Jones T,Jones P,Chen YW,Yokota T

更新日期：2020-07-14 00:00:00

abstract：:Excellent electrophoretic separations of a variety of biological molecules can be accomplished by using uncharged, triblock copolymers as the "gel" media. These copolymers form uncrosslinked, lyotropic liquid crystalline phases of large micelles between which molecules must travel. Unlike crosslinked hydrogels in comm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rill RL,Locke BR,Liu Y,Van Winkle DH

更新日期：1998-02-17 00:00:00

abstract：:The anticancer peptide PNC-27, which contains an HDM-2-binding domain corresponding to residues 12-26 of p53 and a transmembrane-penetrating domain, has been found to kill cancer cells (but not normal cells) by inducing membranolysis. We find that our previously determined 3D structure of the p53 residues of PNC-27 is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sarafraz-Yazdi E,Bowne WB,Adler V,Sookraj KA,Wu V,Shteyler V,Patel H,Oxbury W,Brandt-Rauf P,Zenilman ME,Michl J,Pincus MR

更新日期：2010-02-02 00:00:00

abstract：:It has been known for more than a century that increases in neuronal activity in the brain are reliably accompanied by changes in local blood flow. More recently it has been appreciated that these blood flow increases are accompanied by increases in glycolysis that are much greater than the increases in oxidative phos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vlassenko AG,Rundle MM,Raichle ME,Mintun MA

更新日期：2006-02-07 00:00:00

abstract：:Hyperpolarization of substrates for magnetic resonance spectroscopy (MRS) and imaging (MRI) by dissolution dynamic nuclear polarization (D-DNP) usually involves saturating the ESR transitions of polarizing agents (PAs; e.g., persistent radicals embedded in frozen glassy matrices). This approach has shown enormous pote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gajan D,Bornet A,Vuichoud B,Milani J,Melzi R,van Kalkeren HA,Veyre L,Thieuleux C,Conley MP,Grüning WR,Schwarzwälder M,Lesage A,Copéret C,Bodenhausen G,Emsley L,Jannin S

更新日期：2014-10-14 00:00:00

abstract：:A method is described in which the interference of radiated second-harmonic electric fields generated by a pair of oriented molecules intercalated into double-stranded DNA is controlled and measured. The results show that the relative molecular orientation of the two molecules significantly changes the magnitude of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doughty B,Rao Y,Kazer SW,Kwok SJ,Turro NJ,Eisenthal KB

更新日期：2013-04-09 00:00:00

abstract：:The highly aggressive character of melanoma makes it an excellent model for probing the mechanisms underlying metastasis, which remains one of the most difficult challenges in treating cancer. We find that miR-182, member of a miRNA cluster in a chromosomal locus (7q31-34) frequently amplified in melanoma, is commonly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Segura MF,Hanniford D,Menendez S,Reavie L,Zou X,Alvarez-Diaz S,Zakrzewski J,Blochin E,Rose A,Bogunovic D,Polsky D,Wei J,Lee P,Belitskaya-Levy I,Bhardwaj N,Osman I,Hernando E

更新日期：2009-02-10 00:00:00

abstract：:Transcriptional activation of the HIV type 1 (HIV-1) long terminal repeat (LTR) promoter element by the viral Tat protein is an essential step in the HIV-1 life cycle. Tat function is mediated by the TAR RNA target element encoded within the LTR and is known to require the recruitment of a complex consisting of Tat an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bieniasz PD,Grdina TA,Bogerd HP,Cullen BR

更新日期：1999-07-06 00:00:00

abstract：:Movement over and colonization of surfaces are important survival strategies for bacteria, and many find it advantageous to perform these activities as a group, using quorum sensing to sample population size and synchronize behavior. It is puzzling however, that swarming-proficient and virulent strains of Vibrio parah...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Trimble MJ,McCarter LL

更新日期：2011-11-01 00:00:00