Chymotryptic specificity determinants in the 1.0 A structure of the zinc-inhibited human tissue kallikrein 7.

abstract：:In Saccharomyces cerevisiae cells harboring a GAL1 promoter-linked beta-galactosidase gene, the simultaneous expression of Escherichia coli DNA topoisomerase I and inactivation of yeast DNA topoisomerases I and II reduces the cellular level of beta-galactosidase to an undetectable level. Analysis of intracellular mRNA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gartenberg MR,Wang JC

更新日期：1992-12-01 00:00:00

abstract：:We have used the newly engineered transposable element Dsg to tag a gene that gives rise to a defective kernel (dek) phenotype. Dsg requires the autonomous element Ac for transposition. Upon excision, it leaves a short DNA footprint that can create in-frame and frameshift insertions in coding sequences. Therefore, we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garcia N,Li Y,Dooner HK,Messing J

更新日期：2017-05-16 00:00:00

abstract：:microRNAs comprise a few percent of animal genes and have been recognized as important regulators of a diverse range of biological processes. Understanding the biological functions of miRNAs requires effective means to identify their targets. Combined efforts from computational prediction, miRNA over-expression or dep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong X,Hammell M,Ambros V,Cohen SM

更新日期：2009-09-01 00:00:00

abstract：:Receptor tyrosine kinases (RTKs) are a class of cell surface receptors that, upon ligand binding, stimulate a variety of critical cellular functions. The orphan receptor anaplastic lymphoma kinase (ALK) is one of very few RTKs that remain without a firmly established protein ligand. Here we present a novel cytokine, F...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reshetnyak AV,Murray PB,Shi X,Mo ES,Mohanty J,Tome F,Bai H,Gunel M,Lax I,Schlessinger J

更新日期：2015-12-29 00:00:00

abstract：:The relative abundance of individual proteins shared among clones of lymphocytes provides a meaningful basis for cellular classification. Twelve clones of T cells (obtained by limiting dilution) were analyzed by two-dimensional gel electrophoresis for polypeptide content and then evaluated by the computational techniq...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lefkovits I,Kuhn L,Valiron O,Merle A,Kettman J

更新日期：1988-05-01 00:00:00

abstract：:Emerging evidence suggests that transforming growth factor-beta (TGF-beta) is an important mediator of diabetic nephropathy. We showed previously that short-term treatment with a neutralizing monoclonal anti-TGF-beta antibody (alphaT) in streptozotocin-diabetic mice prevents early changes of renal hypertrophy and incr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ziyadeh FN,Hoffman BB,Han DC,Iglesias-De La Cruz MC,Hong SW,Isono M,Chen S,McGowan TA,Sharma K

更新日期：2000-07-05 00:00:00

abstract：:All immunoglobulins and T-cell receptors throughout phylogeny share regions of highly conserved amino acid sequence. To identify possible primitive immunoglobulins and immunoglobulin-like molecules, we utilized 3' RACE (rapid amplification of cDNA ends) and a highly conserved constant region consensus amino acid seque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berstein RM,Schluter SF,Shen S,Marchalonis JJ

更新日期：1996-04-16 00:00:00

abstract：:The temperature coefficient of equilibrium isotope fractionation in the heavy elements is shown to be larger at high temperatures than that expected from the well-studied vibrational isotope effects. The difference in the isotopic behavior of the heavy elements as compared with the light elements is due to the large n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bigeleisen J

更新日期：1996-09-03 00:00:00

abstract：:ATP is copackaged and coreleased with adrenergic, serotonergic, and cholinergic neurotransmitters, suggesting a possible interaction between the signaling pathways for ATP and these coreleased neurotransmitters. Muscarinic m2 and m4, alpha 2-adrenergic, and D2-dopaminergic neurotransmitter receptors, which have in com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Felder CC,Williams HL,Axelrod J

更新日期：1991-08-01 00:00:00

abstract：:A critical step in the induction of apoptosis is the activation of the apoptotic initiator caspase 9. We show that at its normal physiological concentration, caspase 9 is primarily an inactive monomer (zymogen), and that activity is associated with a dimeric species. At the high concentrations used for crystal formati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renatus M,Stennicke HR,Scott FL,Liddington RC,Salvesen GS

更新日期：2001-12-04 00:00:00

abstract：:Negative elongation factor (NELF) and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) are involved in pausing RNA Polymerase II (Pol II) in the promoter-proximal region of the hsp70 gene in Drosophila, before heat shock induction. Such blocks in elongation are widespread in the Dros...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Missra A,Gilmour DS

更新日期：2010-06-22 00:00:00

abstract：:A membrane-associated folate binding protein (FBP) and a soluble FBP, which is released into the culture medium, have been purified from human KB cells using affinity chromatography. By NaDodSO4/PAGE, both proteins have an apparent Mr of approximately 42,000. However, in the presence of Triton X-100, the soluble FBP e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luhrs CA,Pitiranggon P,da Costa M,Rothenberg SP,Slomiany BL,Brink L,Tous GI,Stein S

更新日期：1987-09-01 00:00:00

abstract：:Local tissue stem cells have been described in airways of the lung but their contribution to normal epithelial maintenance is currently unknown. We therefore developed aggregation chimera mice and a whole-lung imaging method to determine the relative contributions of progenitor (Clara) and bronchiolar stem cells to ep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giangreco A,Arwert EN,Rosewell IR,Snyder J,Watt FM,Stripp BR

更新日期：2009-06-09 00:00:00

abstract：:A Raleigh, NC, population of Drosophila melanogaster was sampled for the presence of enzyme null alleles at 25 loci. No nulls were found at any of five X-linked loci. Nulls were recovered at 13 of 20 autosomal loci; the weighted mean frequency for all 20 autosomal loci was estimated to be 0.0025. A consideration of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Voelker RA,Langley CH,Brown AJ,Ohnishi S,Dickson B,Montgomery E,Smith SC

更新日期：1980-02-01 00:00:00

abstract：:Reduced serum testosterone (T), or hypogonadism, affects millions of men and is associated with many pathologies, including infertility, cardiovascular diseases, metabolic syndrome, and decreased libido and sexual function. Administering T-replacement therapy (TRT) reverses many of the symptoms associated with low T l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li L,Li Y,Sottas C,Culty M,Fan J,Hu Y,Cheung G,Chemes HE,Papadopoulos V

更新日期：2019-11-12 00:00:00

abstract：:Maintenance of genomic stability is essential for normal organismal development and is vital to prevent diseases such as cancer. As genetic information is packaged into chromatin, it has become increasingly clear that the chromatin environment plays an important role in DNA damage response. However, how DNA repair is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Liu L,Yang S,Song N,Zhou X,Gao J,Yu N,Shan L,Wang Q,Liang J,Xuan C,Wang Y,Shang Y,Shi L

更新日期：2014-05-13 00:00:00

abstract：:The refractoriness of acute promyelocytic leukemia (APL) with t(11;17)(q23;q21) to all-trans retinoic acid (ATRA)-based therapy concerns clinicians and intrigues basic researchers. By using a murine leukemic model carrying both promyelocytic leukemia zinc finger/retinoic acid receptor-α (PLZF/RARα) and RARα/PLZF fusio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiao B,Ren ZH,Liu P,Chen LJ,Shi JY,Dong Y,Ablain J,Shi L,Gao L,Hu JP,Ren RB,de Thé H,Chen Z,Chen SJ

更新日期：2013-02-26 00:00:00

abstract：:Plasma membrane insulin receptors, affinity labeled by covalent crosslinking to receptor-bound 125I-labeled insulin, are shown to appear as a heterogeneous population of three major disulfide-linked complexes (Mr 350,000, 320,000, and 290,000) upon electrophoresis in highly porous dodecyl sulfate/polyacrylamide gels i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Massague J,Pilch PF,Czech MP

更新日期：1980-12-01 00:00:00

abstract：:The isolated beta subunit of hemoglobin Kansas has an oxygen affinity that is as low relative to the oxygen affinity of the beta(A) subunit as the affinity of hemoglobin Kansas is low relative to hemoglobin A. Thus the low affinity properties of hemoglobin Kansas are almost completely reflected in the properties of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Riggs A,Gibson QH

更新日期：1973-06-01 00:00:00

abstract：:Enolase genes from land plants and apicomplexa (intracellular parasites, including the malarial parasite, Plasmodium) share two short insertions. This observation has led to the suggestion that the apicomplexan enolase is the product of a lateral transfer event involving the algal endosymbiont from which the apicomple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keeling PJ,Palmer JD

更新日期：2001-09-11 00:00:00

abstract：:Visual receptive fields (RFs) were mapped inside and outside the cortical representation of the optic disk in the striate cortex (area V1) of anesthetized and paralyzed Cebus monkeys. Unexpectedly, most cells were found to be binocularly driven, and the RFs mapped with contralateral-eye stimulation progressed in a top...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fiorani Júnior M,Rosa MG,Gattass R,Rocha-Miranda CE

更新日期：1992-09-15 00:00:00

abstract：:The Cdc7p protein kinase is essential for the G1/S transition and initiation of DNA replication during the cell division cycle in Saccharomyces cerevisiae. Cdc7p appears to be an evolutionarily conserved protein, since a homolog Hsk1 has been isolated from Schizosaccharomyces pombe. Here, we report the isolation of a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiang W,Hunter T

更新日期：1997-12-23 00:00:00

abstract：:In many human cancers, tumor-specific chromosomal rearrangements are known to create chimeric products with the ability to transform cells. The EWS/WT1 protein is such a fusion product, resulting from a t(11;22) chromosomal translocation in desmoplastic small round cell tumors, where 265 aa from the EWS amino terminus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim J,Lee JM,Branton PE,Pelletier J

更新日期：1999-12-07 00:00:00

abstract：:Prions are infectious proteins that encipher biological information within their conformations; variations in these conformations dictate different prion strains. Toward elucidating the molecular language of prion protein (PrP) conformations, we produced an array of recombinant PrP amyloids with varying conformational...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colby DW,Giles K,Legname G,Wille H,Baskakov IV,DeArmond SJ,Prusiner SB

更新日期：2009-12-01 00:00:00

abstract：:The present work shows that arachidonic acid and some other long chain polyunsaturated fatty acids such as docosahexaenoic acid, which is abundant in fish oil, produce a direct open channel block of the major voltage-dependent K+ channel (Kv1.5) cloned in cardiac cells. The inhibitory action of these selected fatty ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Honoré E,Barhanin J,Attali B,Lesage F,Lazdunski M

更新日期：1994-03-01 00:00:00

abstract：:Rare nucleated fetal cells circulate within maternal blood. Noninvasive prenatal diagnosis by isolation and genetic analysis of these cells is currently being undertaken. We sought to determine if genetic evidence existed for persistent circulation of fetal cells from prior pregnancies. Venous blood samples were obtai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bianchi DW,Zickwolf GK,Weil GJ,Sylvester S,DeMaria MA

更新日期：1996-01-23 00:00:00

abstract：:The Drosophila kep1 gene encodes an RNA binding protein related to the murine QUAKING apoptotic inducer. We have previously shown that kep1 can induce apoptosis when transfected into different cell lines. To better define the role of Kep1 in apoptosis, we generated kep1 null flies. These flies were viable, but females...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Di Fruscio M,Styhler S,Wikholm E,Boulanger MC,Lasko P,Richard S

更新日期：2003-02-18 00:00:00

abstract：:Skeletal muscle cells have served as a paradigm for understanding mechanisms leading to cellular differentiation. The proliferation and differentiation of muscle precursor cells require the concerted activity of myogenic regulatory factors including MyoD. In addition, chromatin modifiers mediate dynamic modifications ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ling BM,Bharathy N,Chung TK,Kok WK,Li S,Tan YH,Rao VK,Gopinadhan S,Sartorelli V,Walsh MJ,Taneja R

更新日期：2012-01-17 00:00:00

abstract：:A modification of the polymerase chain reaction has been used to establish the fact that a collection of Staphylococcus aureus toxins are "superantigens," each of which interacts with the T-cell alpha beta receptor of human T cells by means of a specific set of V beta elements. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Choi YW,Kotzin B,Herron L,Callahan J,Marrack P,Kappler J

更新日期：1989-11-01 00:00:00

abstract：:At least two kidney epithelial cell lines, the Madin-Darby canine kidney (MDCK) and the murine inner medullary collecting duct line mIMCD-3, can be induced to form branching tubular structures when cultured with hepatocyte growth factor (HGF) plus serum in collagen I gels. In our studies, whereas MDCK cells remained u...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barros EJ,Santos OF,Matsumoto K,Nakamura T,Nigam SK

更新日期：1995-05-09 00:00:00