Male fetal progenitor cells persist in maternal blood for as long as 27 years postpartum.

Abstract:

:Rare nucleated fetal cells circulate within maternal blood. Noninvasive prenatal diagnosis by isolation and genetic analysis of these cells is currently being undertaken. We sought to determine if genetic evidence existed for persistent circulation of fetal cells from prior pregnancies. Venous blood samples were obtained from 32 pregnant women and 8 nonpregnant women who had given birth to males 6 months to 27 years earlier. Mononuclear cells were sorted by flow cytometry using antibodies to CD antigens 3, 4, 5, 19, 23, 34, and 38. DNA within sorted cells, amplified by PCR for Y chromosome sequences, was considered predictive of a male fetus or evidence of persistent male fetal cells. In the 32 pregnancies, male DNA was detected in 13 of 19 women carrying a male fetus. In 4 of 13 pregnancies with female fetuses, male DNA was also detected. All of the 4 women had prior pregnancies; 2 of the 4 had prior males and the other 2 had terminations of pregnancy. In 6 of the 8 nonpregnant women, male DNA was detected in CD34+CD38+ cells, even in a woman who had her last son 27 years prior to blood sampling. Our data demonstrate the continued maternal circulation of fetal CD34+ or CD34+CD38+ cells from a prior pregnancy. The prolonged persistence of fetal progenitor cells may represent a human analogue of the microchimerism described in the mouse and may have significance in development of tolerance of the fetus. Pregnancy may thus establish a long-term, low-grade chimeric state in the human female.

authors

Bianchi DW,Zickwolf GK,Weil GJ,Sylvester S,DeMaria MA

doi

10.1073/pnas.93.2.705

subject

Has Abstract

pub_date

1996-01-23 00:00:00

pages

705-8

issue

2

eissn

0027-8424

issn

1091-6490

journal_volume

93

pub_type

杂志文章
  • Expression, regulation, and chromosomal localization of the Max gene.

    abstract::The Max gene encodes a protein that interacts specifically with the Myc protein to form a heterodimer with high affinity for the specific cognate DNA binding site of Myc. Here we examine the expression of Max RNA in comparison to Myc RNA during cell growth and differentiation. Two species of RNA, a major 2.0- and a mi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.7.3111

    authors: Wagner AJ,Le Beau MM,Diaz MO,Hay N

    更新日期:1992-04-01 00:00:00

  • Equatorial convergence of India and early Cenozoic climate trends.

    abstract::India's northward flight and collision with Asia was a major driver of global tectonics in the Cenozoic and, we argue, of atmospheric CO(2) concentration (pCO(2)) and thus global climate. Subduction of Tethyan oceanic crust with a carpet of carbonate-rich pelagic sediments deposited during transit beneath the high-pro...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 历史文章,杂志文章

    doi:10.1073/pnas.0805382105

    authors: Kent DV,Muttoni G

    更新日期:2008-10-21 00:00:00

  • Simian virus 40 minichromosomes as targets for retroviral integration in vivo.

    abstract::We present a method for studying multiple retroviral integration events into a small DNA target in vivo. Episomal simian virus 40 (SV40) genomes established by infection of CV-1 cells served as integration targets during subsequent infection with murine leukemia virus (MLV). Using a PCR-based assay for the abundance a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.19.9237

    authors: Pryciak PM,Müller HP,Varmus HE

    更新日期:1992-10-01 00:00:00

  • Transcription program of the chloroplast genome of Euglena gracilis during chloroplast development.

    abstract::RNA transcription in Euglena gracilis chloroplasts has been characterized by hybridization of RNA from cells at different stages of chloroplast development to (3)H-labeled chloroplast DNA restriction endonuclease fragments. Chloroplast DNA was digested into five fragments of 53,000, 35,000, 25,000, 10,000, and 6900 ba...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.5.2258

    authors: Chelm BK,Hallick RB,Gray PW

    更新日期:1979-05-01 00:00:00

  • Increased drought severity tracks warming in the United States' largest river basin.

    abstract::Across the Upper Missouri River Basin, the recent drought of 2000 to 2010, known as the "turn-of-the-century drought," was likely more severe than any in the instrumental record including the Dust Bowl drought. However, until now, adequate proxy records needed to better understand this event with regard to long-term v...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1916208117

    authors: Martin JT,Pederson GT,Woodhouse CA,Cook ER,McCabe GJ,Anchukaitis KJ,Wise EK,Erger PJ,Dolan L,McGuire M,Gangopadhyay S,Chase KJ,Littell JS,Gray ST,St George S,Friedman JM,Sauchyn DJ,St-Jacques JM,King J

    更新日期:2020-05-26 00:00:00

  • Trafficking of gap junction channels at a vertebrate electrical synapse in vivo.

    abstract::Trafficking and turnover of transmitter receptors required to maintain and modify the strength of chemical synapses have been characterized extensively. In contrast, little is known regarding trafficking of gap junction components at electrical synapses. By combining ultrastructural and in vivo physiological analysis ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1121557109

    authors: Flores CE,Nannapaneni S,Davidson KG,Yasumura T,Bennett MV,Rash JE,Pereda AE

    更新日期:2012-02-28 00:00:00

  • Selection of the lamprey VLRC antigen receptor repertoire.

    abstract::The alternative adaptive immune system of jawless vertebrates is based on different isotypes of variable lymphocyte receptors (VLRs) that are composed of leucine-rich repeats (LRRs) and expressed by distinct B- and T-like lymphocyte lineages. VLRB is expressed by B-like cells, whereas VLRA and VLRC are expressed by tw...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1415655111

    authors: Holland SJ,Gao M,Hirano M,Iyer LM,Luo M,Schorpp M,Cooper MD,Aravind L,Mariuzza RA,Boehm T

    更新日期:2014-10-14 00:00:00

  • Excitatory monocyte chemoattractant protein-1 signaling is up-regulated in sensory neurons after chronic compression of the dorsal root ganglion.

    abstract::Neuronal hyperexcitability in both injured and adjacent uninjured neurons is associated with states of chronic injury and pain and is likely subject to neuroinflammatory processes. Chronic inflammatory responses are largely orchestrated by chemokines. One chemokine, monocyte chemoattractant protein-1 (MCP-1), in the p...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0503496102

    authors: White FA,Sun J,Waters SM,Ma C,Ren D,Ripsch M,Steflik J,Cortright DN,Lamotte RH,Miller RJ

    更新日期:2005-09-27 00:00:00

  • Spatially clustered neuronal assemblies comprise the microstructure of synchrony in chronically epileptic networks.

    abstract::Epilepsy is characterized by recurrent synchronizations of neuronal activity, which are both a cardinal clinical symptom and a debilitating phenomenon. Although the temporal dynamics of epileptiform synchronizations are well described at the macroscopic level using electrophysiological approaches, less is known about ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1216958110

    authors: Feldt Muldoon S,Soltesz I,Cossart R

    更新日期:2013-02-26 00:00:00

  • Differential fitness costs of reproduction between the sexes.

    abstract::Natural selection does not necessarily favor maximal reproduction because reproduction imposes fitness costs, reducing parental survival, and offspring quality. Here, we show that parents in a preindustrial population in North America incurred fitness costs from reproduction, and women incurred greater costs than men....

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 历史文章,杂志文章

    doi:10.1073/pnas.0609301103

    authors: Penn DJ,Smith KR

    更新日期:2007-01-09 00:00:00

  • Ubiquitin-conjugating enzyme Ubc13 is a critical component of TNF receptor-associated factor (TRAF)-mediated inflammatory responses.

    abstract::Ubc13 is a ubiquitin-conjugating enzyme responsible for noncanonical ubiquitination of TNF receptor-associated factor (TRAF)-family adapter proteins involved in Toll-like receptor and TNF-family cytokine receptor signaling, which are regulators of innate immunity. Gene ablation was used to study the function of Ubc13 ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0700548104

    authors: Fukushima T,Matsuzawa S,Kress CL,Bruey JM,Krajewska M,Lefebvre S,Zapata JM,Ronai Z,Reed JC

    更新日期:2007-04-10 00:00:00

  • Partial deletion of beta-globin gene DNA in certain patients with beta 0-thalassemia.

    abstract::We have used restriction endonuclease mapping of cell DNA to investigate the structure of the beta-globin gene in beta-thalassemias. Among 17 individuals with beta +- and beta 0-thalassemia, we observed three patients of Indian origin with beta 0-thalassemia whose DNA revealed a consistent mapping abnormality. In one ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.5.2400

    authors: Orkin SH,Old JM,Weatherall DJ,Nathan DG

    更新日期:1979-05-01 00:00:00

  • Overexpression of Cu-Zn superoxide dismutase in Drosophila does not affect life-span.

    abstract::Aging and disease processes may be due to deleterious and irreversible changes produced by free radical reactions. The enzyme copper-zinc superoxide dismutase (Cu-Zn SOD; superoxide:superoxide oxidoreductase, EC 1.15.1.1) performs a protective function by scavenging superoxide radicals. The Cu-Zn SOD gene (Sod) cloned...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.11.4270

    authors: Seto NO,Hayashi S,Tener GM

    更新日期:1990-06-01 00:00:00

  • Facial color is an efficient mechanism to visually transmit emotion.

    abstract::Facial expressions of emotion in humans are believed to be produced by contracting one's facial muscles, generally called action units. However, the surface of the face is also innervated with a large network of blood vessels. Blood flow variations in these vessels yield visible color changes on the face. Here, we stu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1716084115

    authors: Benitez-Quiroz CF,Srinivasan R,Martinez AM

    更新日期:2018-04-03 00:00:00

  • Spatial mapping of polymicrobial communities reveals a precise biogeography associated with human dental caries.

    abstract::Tooth decay (dental caries) is a widespread human disease caused by microbial biofilms. Streptococcus mutans, a biofilm-former, has been consistently associated with severe childhood caries; however, how this bacterium is spatially organized with other microorganisms in the oral cavity to promote disease remains unkno...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1919099117

    authors: Kim D,Barraza JP,Arthur RA,Hara A,Lewis K,Liu Y,Scisci EL,Hajishengallis E,Whiteley M,Koo H

    更新日期:2020-06-02 00:00:00

  • Partial reaction of peptide initiation inhibited by phosphorylation of either initiation factor eIF-2 or 40S ribosomal proteins.

    abstract::Preparations of the hemin-controlled repressor (HCR) from rabbit reticulocytes contain 3':5'-cyclic-AMP-independent protein kinase activity for the smallest subunit of the peptide initiation factor eIF-2 and for proteins of reticulocyte 40S ribosomal subunits. Binding of the ternary complex formed between Met-tRNAf, G...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.74.4.1445

    authors: Kramer G,Henderson AB,Pinphanichakarn P,Wallis MH,Hardesty B

    更新日期:1977-04-01 00:00:00

  • Distribution of ions around DNA, probed by energy transfer.

    abstract::Measurements of the effect of DNA on rates of bimolecular energy transfer between ions provide a direct indication of how cations cluster in regions near DNA and how anions are repelled from the same regions. Energy transfer from luminescent lanthanide ions (in the "rapid-diffusion" limit) probes collision frequencies...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.10.3267

    authors: Wensel TG,Meares CF,Vlachy V,Matthew JB

    更新日期:1986-05-01 00:00:00

  • Epigenomic analysis of Alu repeats in human ependymomas.

    abstract::Global loss of DNA methylation has been known for decades as an epigenomic aberration associated with carcinogenesis and cancer progression. Loss of DNA methylation affects predominantly repetitive elements, which encompass >50% of the CpG dinucleotides present in the human genome. Because of the lack of an effective ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0913836107

    authors: Xie H,Wang M,Bonaldo Mde F,Rajaram V,Stellpflug W,Smith C,Arndt K,Goldman S,Tomita T,Soares MB

    更新日期:2010-04-13 00:00:00

  • Near-infrared fluorescent sensor for in vivo copper imaging in a murine Wilson disease model.

    abstract::Copper is an essential metal nutrient that is tightly regulated in the body because loss of its homeostasis is connected to severe diseases such as Menkes and Wilson diseases, Alzheimer's disease, prion disorders, and amyotrophic lateral sclerosis. The complex relationships between copper status and various stages of ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1113729109

    authors: Hirayama T,Van de Bittner GC,Gray LW,Lutsenko S,Chang CJ

    更新日期:2012-02-14 00:00:00

  • Structure of a single-chain antibody variable domain (Fv) fragment complexed with a carbohydrate antigen at 1.7-A resolution.

    abstract::We describe here the 1.7-A resolution structure of a single-chain antibody variable domain (scFv) molecule, based on the carbohydrate-binding antibody Se155-4, complexed with the trisaccharide ligand alpha-D-Gal(1-->2)[alpha-D-Abe(1-->3)]alpha-D-Manp1-->OMe, where Abe is abequose. The scFv expressed in Escherichia col...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.14.6423

    authors: Zdanov A,Li Y,Bundle DR,Deng SJ,MacKenzie CR,Narang SA,Young NM,Cygler M

    更新日期:1994-07-05 00:00:00

  • Recombinant human migration inhibitory factor has adjuvant activity.

    abstract::Recombinant human migration inhibitory factor (MIF), isolated through functional expression cloning in COS-1 cells, up-regulates expression of genes encoding HLA-DR and interleukin 1 beta (IL-1 beta) and elaboration of IL-1 beta by human monocyte-derived macrophages. Administration of soluble bovine serum albumin or h...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章,收录出版

    doi:10.1073/pnas.89.17.8049

    authors: Weiser WY,Pozzi LM,Titus RG,David JR

    更新日期:1992-09-01 00:00:00

  • High-order fractal states in graphene superlattices.

    abstract::Graphene superlattices were shown to exhibit high-temperature quantum oscillations due to periodic emergence of delocalized Bloch states in high magnetic fields such that unit fractions of the flux quantum pierce a superlattice unit cell. Under these conditions, semiclassical electron trajectories become straight agai...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1804572115

    authors: Krishna Kumar R,Mishchenko A,Chen X,Pezzini S,Auton GH,Ponomarenko LA,Zeitler U,Eaves L,Fal'ko VI,Geim AK

    更新日期:2018-05-15 00:00:00

  • Quality-control mechanisms targeting translationally stalled and C-terminally extended poly(GR) associated with ALS/FTD.

    abstract::Maintaining the fidelity of nascent peptide chain (NP) synthesis is essential for proteome integrity and cellular health. Ribosome-associated quality control (RQC) serves to resolve stalled translation, during which untemplated Ala/Thr residues are added C terminally to stalled peptide, as shown during C-terminal Ala ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2005506117

    authors: Li S,Wu Z,Tantray I,Li Y,Chen S,Dong J,Glynn S,Vogel H,Snyder M,Lu B

    更新日期:2020-10-06 00:00:00

  • Dissociation of human mid-dorsolateral from posterior dorsolateral frontal cortex in memory processing.

    abstract::Work with non-human primates had previously demonstrated that the mid-dorsolateral frontal cortex, which comprises cytoarchitectonic areas 46 and 9, plays a critical role in the performance of non-spatial self-ordered working memory tasks, whereas the immediately adjacent posterior dorsolateral frontal cortex (area 8)...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.3.873

    authors: Petrides M,Alivisatos B,Evans AC,Meyer E

    更新日期:1993-02-01 00:00:00

  • Proximity of the manganese cluster of photosystem II to the redox-active tyrosine YZ.

    abstract::Electron spin echo electron-nuclear double resonance (ESE-ENDOR) experiments performed on a broad radical electron paramagnetic resonance (EPR) signal observed in photosystem II particles depleted of Ca2+ indicate that this signal arises from the redox-active tyrosine YZ. The tyrosine EPR signal width is increased rel...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.21.9545

    authors: Gilchrist ML Jr,Ball JA,Randall DW,Britt RD

    更新日期:1995-10-10 00:00:00

  • Identification of a serotonin receptor coupled to adenylyl cyclase involved in learning-related heterosynaptic facilitation in Aplysia.

    abstract::Serotonin (5-HT) plays a critical role in modulating synaptic plasticity in the marine mollusc Aplysia and in the mammalian nervous system. In Aplysia sensory neurons, 5-HT can activate several signal cascades, including PKA and PKC, presumably via distinct types of G protein-coupled receptors. However, the molecular ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0907502106

    authors: Lee YS,Choi SL,Lee SH,Kim H,Park H,Lee N,Lee SH,Chae YS,Jang DJ,Kandel ER,Kaang BK

    更新日期:2009-08-25 00:00:00

  • Quantifying and mapping the human appropriation of net primary production in earth's terrestrial ecosystems.

    abstract::Human appropriation of net primary production (HANPP), the aggregate impact of land use on biomass available each year in ecosystems, is a prominent measure of the human domination of the biosphere. We present a comprehensive assessment of global HANPP based on vegetation modeling, agricultural and forestry statistics...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0704243104

    authors: Haberl H,Erb KH,Krausmann F,Gaube V,Bondeau A,Plutzar C,Gingrich S,Lucht W,Fischer-Kowalski M

    更新日期:2007-07-31 00:00:00

  • Size-based molecular diagnostics using plasma DNA for noninvasive prenatal testing.

    abstract::Noninvasive prenatal testing using fetal DNA in maternal plasma is an actively researched area. The current generation of tests using massively parallel sequencing is based on counting plasma DNA sequences originating from different genomic regions. In this study, we explored a different approach that is based on the ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1406103111

    authors: Yu SC,Chan KC,Zheng YW,Jiang P,Liao GJ,Sun H,Akolekar R,Leung TY,Go AT,van Vugt JM,Minekawa R,Oudejans CB,Nicolaides KH,Chiu RW,Lo YM

    更新日期:2014-06-10 00:00:00

  • Quantifying structure and performance diversity for sets of small molecules comprising small-molecule screening collections.

    abstract::Using a diverse collection of small molecules we recently found that compound sets from different sources (commercial; academic; natural) have different protein-binding behaviors, and these behaviors correlate with trends in stereochemical complexity for these compound sets. These results lend insight into structural ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1015024108

    authors: Clemons PA,Wilson JA,Dančík V,Muller S,Carrinski HA,Wagner BK,Koehler AN,Schreiber SL

    更新日期:2011-04-26 00:00:00

  • Metabolism of benzo[a]pyrene by human mammary epithelial cells: toxicity and DNA adduct formation.

    abstract::Pure cultures of human breast epithelial cells and of fibroblastic cells in early passage provided the opportunity to ask whether either cell type had the capability for metabolizing chemical carcinogens and, if so, was the fate of the metabolic products compatible with chemical carcinogens being a factor in the initi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.10.6251

    authors: Stampfer MR,Bartholomew JC,Smith HS,Bartley JC

    更新日期:1981-10-01 00:00:00